scholarly journals Chemotactic behavior of Campylobacter fetus subspecies towards cervical mucus, bovine placenta and selected substances and ion

2021 ◽  
Vol 18 (2) ◽  
Author(s):  
Dionei Joaquim Haas ◽  
Jonata de Melo Barbieri ◽  
Elaine Maria Seles Dorneles ◽  
Andrey Pereira Lage
Keyword(s):  
Cervical Mucus ◽  
Bovine Placenta ◽  
Campylobacter Fetus ◽  
Chemotactic Behavior

scholarly journals Identification of Campylobacter fetus subsp. venerealis virulence genes in cervical mucus from cows

2019 ◽  
Vol 50 (4) ◽  
pp. 1133-1137 ◽  
Author(s):  
Érica Chaves Lúcio ◽  
Mércia Rodrigues Barros ◽  
Rinaldo Aparecido Mota ◽  
Rita de Cássia Carvalho Maia ◽  
José Wilton Pinheiro
Keyword(s):  
Virulence Genes ◽  
Cervical Mucus ◽  
Campylobacter Fetus ◽  
Fetus Subsp

scholarly journals The comparison of pro- and antioxidative parameters in plasma and placental tissues during early phase of placental development in cows

2021 ◽  
Author(s):  
Jacek Wawrzykowski ◽  
Monika Jamioł ◽  
Wioleta Mojsym ◽  
Marta Kankofer
Keyword(s):  
Blood Plasma ◽  
Antioxidative Enzymes ◽  
Placental Development ◽  
Blood Samples ◽  
Bovine Placenta ◽  
Total Antioxidant ◽  
Tissue Homogenates ◽  
Further Development ◽  
General Profile ◽  
In Blood Plasma

AbstractPhysiological balance between pro- and antioxidative processes is crucial for placentation and further development of fetus and placenta. Parameters of pro- and antioxidative profile may serve as markers of proper course of pregnancy. The aim of study was to assess whether the balance between pro- and antioxidative parameters during placentation phase in bovine placenta is maintained. Placental and blood samples were collected from healthy, HF, pregnant (2nd-3rd month) cows (n = 8) in slaughterhouse and in farm, respectively. Formylokinurenine and bityrosine content were measured spectrofluorimetrically in blood plasma and tissue homogenates while metabolites of lipid peroxidation, total antioxidant capacity, SH groups and activity of antioxidative enzymes (glutathione peroxidase and superoxide dismutase) were determined in examined tissues by spectrophotometry. Western blotting was used to confirm the presence of enzymatic proteins in placenta. Results: Local profile in tissues was more pronounced than general profile in blood plasma. Activities of antioxidative enzymes were significantly (p < 0.05) higher in 2nd compared to 3rd month of pregnancy in maternal part of placenta while prooxidant parameters showed opposite relationship. Obtained results showed significant differences when compared to data from non-pregnant animals or time of parturition. Further studies are necessary for elucidation of placentation phase in cows.

Characterisation of sialic acid species in sheep cervical mucus

2020 ◽  
Vol 220 ◽  
pp. 106361
Author(s):  
Laura Abril-Parreño ◽  
Jack Morgan ◽  
Anette Krogenæs ◽  
Xavier Druart ◽  
Mary E. Gallagher ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cervical Mucus

scholarly journals The Detection of Bovine Estrus by Lactoferrin Monoclonal Antibody

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1582
Author(s):  
Jihwan Lee ◽  
Suhyun Lee ◽  
Younbae Park ◽  
Seokhyun Lee ◽  
Seungmin Ha ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Binding Affinity ◽  
Reproductive Performance ◽  
Economic Loss ◽  
Cervical Mucus ◽  
Female Rats ◽  
Economic Damage ◽  
Bovine Lactoferrin ◽  
Estrous Synchronization ◽  
High Binding Affinity

To improve reproductive performance in cattle, the accurate detection of estrus and optimization of insemination relative to ovulation are necessary. However, poor heat detection by farm staff leads to a decreased conception rate, thus inflicting economic damage to the beef and dairy industries. This study aimed to develop monoclonal antibodies (mAb) that can specifically bind to the bovine lactoferrin (bLF) protein, which we have previously demonstrated to be overexpressed in bovine cervical mucus during estrus. Female rats were intraperitoneally immunized with bLF protein as the antigen. Anti-bLF mAbs were then purified by affinity chromatography, and their binding affinity for the bLF antigen was examined using ELISA. We found a high binding affinity between mAbs and bLF. Finally, we developed a rapid bovine heat detection kit using the anti-bLF mAbs that we generated and tested on cervical mucus from 12 cows (estrous synchronization, n = 2; natural cycling, n = 10). We found that the kits accurately detected estrus. Overall, our fabricated heat detection kit based on rat anti-bLF mAbs could pave the way for the development of potent tools for heat detection devices for dairy cattle, thereby preventing economic loss.

scholarly journals Use of fertility awareness methods as a component of safer conception for women in HIV-serodifferent relationships in Kenya

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Yasaman Zia ◽  
Jennifer Velloza ◽  
Lynda Oluoch ◽  
Richard Momanyi ◽  
Sarah Mbugua ◽  
...  
Keyword(s):  
Hiv Transmission ◽  
Proportional Hazards ◽  
Cox Proportional Hazards ◽  
Cervical Mucus ◽  
Safer Conception ◽  
Open Label ◽  
Fertility Awareness ◽  
Menstrual Cycles ◽  
Condomless Sex ◽  
Message Service

Abstract Background For couples affected by HIV, and serodifferent couples in particular, pregnancy desire is often juxtaposed against the risk of HIV transmission between the couple and the potential neonate leading to thinking about measures to minimize risk of HIV transmission. We assess the use of fertility awareness methods [FAM] and evaluate the drivers of alignment between indicators of fertility and sexual behavior among HIV-serodifferent couples desiring pregnancy. Methods HIV-serodifferent couples from Thika, Kenya were enrolled into an open-label pilot evaluation of safer conception strategies. Women responded to daily 7-item short message service [SMS] surveys on FAM and sexual activity. Menstrual cycles were categorized as having condomless sex aligned, not aligned, or partially aligned to the predicted peak fertility. We used binomial logit models with generalized estimating equations to assess alignment between condomless sex during peak fertility days and FAM results. We used Cox proportional hazards to compare pregnancy incidence among months with sex and peak fertility aligned and mis-aligned. Results A total of 6929 SMS surveys across 252 menstrual cycles of 65 women were included. Reporting “sticky” cervical mucus (adjusted odds ratio [aOR]: 2.25, 95% confidence interval [95% CI]: 1.30, 3.90) and positive ovulation prediction kit [OPK] result (aOR: 2.07, 95% CI: 1.11, 3.86) were associated with increased likelihood of alignment of condomless sex during peak fertility. Pregnancy incidence was statistically similar among periods with sex aligned and not aligned with peak fertility. Conclusions Among women engaged in a comprehensive safer conception program, a moderate percentage of women aligned condomless sex and predicted peak fertility days at least once. While FAM, particularly cervical mucus and OPK, are an inexpensive option for couples to consider using as a component of their safer conception strategies, antiretroviral-based strategies remain important to minimize risk.

scholarly journals Movements of HIV-Virions in Human Cervical Mucus

2009 ◽  
Vol 10 (9) ◽  
pp. 2482-2488 ◽  
Author(s):  
Hacène Boukari ◽  
Beda Brichacek ◽  
Pamela Stratton ◽  
Sheila F. Mahoney ◽  
Jeffrey D. Lifson ◽  
...  
Keyword(s):  
Cervical Mucus

Production of cervical mucus in ewes treated with exogenous progestagen and oestrogen

1972 ◽  
Vol 23 (3) ◽  
pp. 473 ◽  
Author(s):  
AJ Allison
Keyword(s):  
Intravenous Administration ◽  
Linear Response ◽  
Cervical Mucus ◽  
Exogenous Oestrogen ◽  
Oestradiol Benzoate ◽  
Cessation Of Treatment

The production of cervical mucus was measured at intervals of 6 hr about the onset of oestrus in ewes treated with exogenous progestagen administered by the intramuscular or intravaginal route. There was a quadratic pattern of production with peak values early in the oestrous period. Although there were no differences between groups in the total amount of mucus produced, levels recorded in untreated ewes were lower in the pre-oestrous period. Within the intravaginal progestagen group, those ewes which exhibited oestrus within 42 hr of sponge withdrawal produced greater quantities of cervical mucus than did animals which came into heat after this time. This relationship was not evident within the intramuscular progesterone group. It was concluded that the variable cervical mucus response observed after the cessation of treatment may contribute to the lowered fertility commonly observed following the synchronization of oestrus. The cervical mucus response to injected steroids was recorded in spayed ewes. This was a specific linear response to exogenous oestrogen. Progesterone priming resulted in higher levels of cervical secretions from 24 to 42 hr after the administration of oestradiol benzoate (ODB). Intravenous administration of ODB caused an earlier but short-lived response.

Changes in the composition of cervical mucus of the cow during the estrous cycle as parameters for predicting potential fertility

1993 ◽  
Vol 39 (3) ◽  
pp. 569-580 ◽  
Author(s):  
D Zaaijer ◽  
G.H.M. Counotte ◽  
J Sol ◽  
W.J. Smidt ◽  
P.J. Broadbent
Keyword(s):  
Estrous Cycle ◽  
Cervical Mucus
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