scholarly journals Inheritance of resistance to papaya ringspot virus-watermelon strain (PRSV-W) in 'Whitaker' summer squash line

2015 ◽  
Vol 15 (4) ◽  
pp. 203-209 ◽  
Author(s):  
Cícero B Menezes ◽  
Wilson R Maluf ◽  
Marcos V Faria ◽  
Sebastião M Azevedo ◽  
Juliano T V Resende ◽  
...  
Keyword(s):  
Genetic Control ◽  
Cucurbita Pepo ◽  
Major Gene ◽  
Ringspot Virus ◽  
Inheritance Of Resistance ◽  
Papaya Ringspot Virus ◽  
Broad Sense Heritability ◽  
Major Gene Effect ◽  
Summer Squash ◽  
Parental Lines

Abstract The objective of this work was to study the genetic control of the PRSV-W (Papaya Ringspot Virus - Watermelon Strain) resistance in Cucurbita pepo 'Whitaker' line. Plants of parental lines Whitaker (resistant) and Caserta (susceptible), and of the generations F1, F2, BC11 and BC12 were evaluated for their reactions to PRSV-W. Caserta plants showed severe mosaic symptoms, while Whitaker grew vigorously and remained almost totally symptom-free. Most of the F1, F2 and backcross plants also presented severe mosaic symptoms. Data were used to test a hypothesis of monogenic inheritance under different presumed degrees of dominance, and genetic models were tested using maximum likelihood tests of genetic control. Broad-sense heritability was of 0.57 for the first evaluation. Resistance to PRSV-W in C. pepo 'Whitaker' is due to a major gene effect summed to polygenic effects.

scholarly journals Complete Genome Sequence of a Papaya ringspot virus Isolate from South Korea That Infects Cucurbita pepo

2017 ◽  
Vol 5 (48) ◽  
Author(s):  
Dasom Baek ◽  
Davaajargal Igori ◽  
Seungmo Lim ◽  
Un Sun Hwang ◽  
Eung Kyoo Choi ◽  
...  
Keyword(s):  
South Korea ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Cucurbita Pepo ◽  
Virus Isolate ◽  
Ringspot Virus ◽  
Full Genome Sequence ◽  
Papaya Ringspot Virus ◽  
Full Genome

ABSTRACT The complete genome sequence of a Papaya ringspot virus (PRSV) isolate from South Korea (SK) infecting squash (Cucurbita pepo) was obtained using paired-end RNA sequencing. A BLASTn search of the PRSV SK isolate full-genome sequence showed nucleotide sequence identity ranging from 81% to 83% with previously reported PRSV isolates (GenBank accession numbers KX655874 and EF017707).

scholarly journals Inheritance of Resistance to the Watermelon Strain of Papaya Ringspot Virus in Watermelon

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 811C-811
Author(s):  
Nihat Guner* ◽  
Zvezdana Pesic-VanEsbroeck ◽  
Todd Wehner
Keyword(s):  
New Hampshire ◽  
Citrullus Lanatus ◽  
Recessive Gene ◽  
Germplasm Collection ◽  
Ringspot Virus ◽  
Inheritance Of Resistance ◽  
Papaya Ringspot Virus ◽  
Single Recessive Gene ◽  
Susceptible Parent ◽  
Sources Of Resistance

Sources of resistance to the watermelon strain of papaya ringspot virus (PRSV-W) have been identified within the watermelon (Citrullus lanatus) germplasm collection. Inheritance of resistance to papaya ringspot virus-watermelon strain was studied in three C. lanatus var. citroides accessions: PI 244017, PI 244019, and PI 485583. The susceptible parent lines `Allsweet', `Calhoun Gray', and `New Hampshire Midget' were crossed with resistant accessions to develop F1, F2, and BC1 generations for six families. A single recessive gene was found to control resistance to PRSV-W. The gene symbol `prv' is proposed for PRSV-W resistance in watermelon. Additional work is needed to determine whether the genes in PI 244017, PI 244019, and PI 485583 are allelic for resistance to PRSV-W.

scholarly journals Yield and Virus Resistance of Summer Squash Cultivars and Breeding Lines in North Carolina

1998 ◽  
Vol 8 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Jonathan R. Schultheis ◽  
S. Alan Walters
Keyword(s):  
North Carolina ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Breeding Lines ◽  
Summer Squash

Yellow and zucchini squash (Cucurbita pepo L.) cultigens (breeding lines and cultivars) were evaluated over a 2-year (1995 and 1996) period in North Carolina. Yellow squash cultigens that performed well (based on total marketable yields) were `Destiny III', `Freedom III', `Multipik', XPHT 1815, and `Liberator III' in Fall 1995 and HMX 4716, `Superpik', PSX 391, `Monet', `Dixie', XPH 1780, and `Picasso' in Spring 1996. Some of the yellow squash cultigens evaluated had superior viral resistance: XPHT 1815, XPHT 1817, `Freedom III', `Destiny III', `Freedom II', TW 941121, `Prelude II', and `Liberator III' in Fall 1995 and XPHT 1815, `Liberator III', `Prelude II', and `Destiny III' in Fall 1996; all these cultigens were transgenic. The yellow squash cultigens that performed well (based on total marketable yields) in the Fall 1995 test had transgenic virus resistance (`Destiny III', `Freedom III', XPHT 1815, and `Liberator III') or had the Py gene present in its genetic background (`Multipik'). Based on total marketable yields, the best zucchini cultigens were XPHT 1800, `Tigress', XPHT 1814, `Dividend' (ZS 19), `Elite', and `Noblesse' in Fall 1995; and `Leonardo', `Tigress', `Hurricane', `Elite', and `Noblesse' in Spring 1996. The zucchini cultigens with virus resistance were TW 940966, XPHT 1814, and XPHT 1800 in Fall 1995 and XPHT 1800, XPHT 1776, XPHT 1777, XPHT 1814, and XPHT 1784 in Fall 1996. Even though TW 940966 had a high level of resistance in the Fall 1995 test, it was not as high yielding as some of the more susceptible lines. Viruses detected in the field were papaya ringspot virus (PRSV) and watermelon mosaic virus (WMV) for Fall 1995; while PRSV, zucchini yellow mosaic virus (ZYMV), and WMV were detected for Fall 1996. Summer squash cultigens transgenic for WMV and ZYMV have potential to improve yield, especially during the fall when viruses are more prevalent. Most transgenic cultigens do not possess resistance to PRSV, except XPHT 1815 and XPHT 1817. Papaya ringspot virus was present in the squash tests during the fall of both years. Thus, PRSV resistance must be transferred to the transgenic cultigens before summer squash can be grown during the fall season without the risk of yield loss due to viruses.

scholarly journals Danos na produção da abobrinha de moita causados pelo Papaya ringspot virus type W e Zucchini yellow mosaic virus

2007 ◽  
Vol 33 (2) ◽  
pp. 192-194 ◽  
Author(s):  
Mônica Juliani Zavaglia Pereira ◽  
Angelo Aparecido Barbosa Sussel ◽  
Ricardo Ferreira da Silva ◽  
Odair José Kuhn ◽  
Fernanda Domingues ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

Este trabalho teve por objetivo avaliar, em condições de casa de vegetação e de campo, os danos causados pelo PRSV-W e ZYMV em abobrinha-de-moita (Cucurbita pepo cv. Caserta). As plantas em casa de vegetação foram inoculadas com os vírus individualmente e em mistura aos 12 e 22 dias após emergência (DAE) e aos 5, 15 e 25 DAE no campo. Em casa de vegetação, as infecções com PRSV-W + ZYMV, PRSV-W e ZYMV, na primeira época de inoculação, ocasionaram reduções de área foliar de 39,6%, 36,8% e 12,1%, respectivamente. As massas fresca e seca também foram significativamente afetadas na primeira época de inoculação. No campo, as plantas com infecções individuais ou mistas dos potyvírus produziram frutos não comerciais em quantidades que variaram de 14 a 861 g/planta, dependendo da idade que foram inoculadas. As plantas tratadas com tampão fosfato aos 5, 15 e 25 DAE produziram em média 573 g, 937 g e 1172 g de frutos comerciais e 282 g, 221 g e 192 g de frutos não comerciais, respectivamente. A redução na massa fresca das plantas foi diretamente relacionada com a época de inoculação, com médias de 60,7% para aquelas inoculadas aos 5 DAE e de 22,7% para aquelas inoculadas aos 15 DAE. Na terceira época de inoculação não houve diferença significativa de massa fresca entre os tratamentos.

scholarly journals Inheritance of Resistance to Colletotrichum Species in Strawberry

1991 ◽  
Vol 116 (4) ◽  
pp. 724-727 ◽  
Author(s):  
Creighton L. Gupton ◽  
Barbara J. Smith
Keyword(s):  
Recurrent Selection ◽  
Major Gene ◽  
Broad Sense ◽  
Inheritance Of Resistance ◽  
Broad Sense Heritability ◽  
Narrow Sense ◽  
Narrow Sense Heritability ◽  
Genetic Variances ◽  
Heritability Estimates ◽  
Selection Of

Experiments were conducted to estimate the relative importance of additive and dominance genetic variances and non-allelic interactions in the inheritance of resistance to Colletotrichum spp. in strawberry (Fragaria × ananassa Duch.). Progeny of 40 parents crossed in a Comstock and Robinson Design II Mating scheme were inoculated with three isolates of C. fragariae and one isolate of C. acutatum. Disease development on each plant was rated visually. Variance components were estimated and converted to genetic variances. Estimates of were six to 10 times higher than those for Within-family variance not accounted for by equaled 35% and 38% of the total genetic variance in females and males, respectively, indicating probable epistatic effects. The frequency distribution of disease severity ratings was bimodal in both experiments, suggesting major gene action. Narrow-sense heritability estimates were 0.37 and 0.26, and broad-sense heritability estimates were 0.87 and 0.85 for females and males, respectively. Narrow-sense heritability estimates are probably sufficient to produce gains from recurrent selection. Gains from selection of clonal value should be possible because of the high broad sense heritability estimates. It appears feasible to establish a broad genetic-based population resistant to Colletotrichum spp. from which selections could be evaluated per se and/or recombined to produce improved populations.

Inheritance of resistance to papaya ringspot virus type W in hybrids between Cucurbita ecuadorensis and C. maxima

1989 ◽  
Vol 29 (2) ◽  
pp. 253 ◽  
Author(s):  
ME Herrington ◽  
DE Byth ◽  
DS Teakle ◽  
PJ Brown
Keyword(s):  
Virus Type ◽  
Ringspot Virus ◽  
Inheritance Of Resistance ◽  
Papaya Ringspot Virus ◽  
Cucurbita Maxima ◽  
Gene Effects ◽  
Analysis Of Means ◽  
Backcross Populations ◽  
Additive Gene ◽  
Polygenic Analysis

Papaya ringspot virus type W (PRV-W) causes foliar mosaic, deformed fruit and reduced yield in crops of Cucurbita maxima cv. Queensland Blue in Queensland. Cucurbita ecuadorensis is highly resistant to PRV-W. Using crosses between these 2 cucurbits we studied inheritance of resistance to PRV-W in mechanically inoculated parental, F1, F2, backcross and inbred backcross populations under glasshouse and/or field conditions. Disease severity was assessed on each plant as the proportion of the young leaf area which was chlorotic. Inheritance of resistance, following inoculation of cotyledons with either of 2 isolates that differed in the severity of symptoms produced, was most consistently described as polygenic. Analysis of means of generations indicated additive gene effects were predominant. However simpler inheritance could not be entirely discarded as a 13:3 digenic model based on epistasis and modifiers described some distributions. No strong associations existed between high resistance to PRV-W and undesirable characteristics so that transfer of high levels of resistance from C. ecuadorensis to commercially acceptable lines of C. maxima should be possible.

scholarly journals Efeito de diferentes variáveis na infetividade de estirpes fracas do Papaya ringspot virus em plantas de melancia

2005 ◽  
Vol 30 (1) ◽  
pp. 39-45
Author(s):  
José Segundo Giampan ◽  
Jorge Alberto M. Rezende
Keyword(s):  
Virus Type ◽  
Cucurbita Pepo ◽  
Citrullus Lanatus ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

Este trabalho teve como objetivo inicial selecionar uma estirpe fraca do Papaya ringspot virus - type W (PRSV-W) de bolhas de folhas de melancia (Citrullus lanatus) com mosaico. A infetividade dessa estirpe, PRSV-W-3, em plantas de melancia foi comparada com a das estirpes fracas PRSV-W-1 e PRSV-W-2, previamente selecionadas de bolhas de folhas de abobrinha de moita (Cucurbita pepo) cv. Caserta com mosaico. Além do efeito da origem da estirpe fraca na infetividade de plantas de melancia, avaliaram-se, ainda, os efeitos da concentração do inóculo, da espécie da planta fonte do inóculo e da idade da planta de melancia inoculada. Como controle, foi utilizada uma estirpe severa do vírus obtida de abobrinha de moita (PRSV-W-C). A avaliação do efeito da concentração e da espécie da planta fonte do inóculo foi feita com extratos de quatro, oito, 12 e 16 discos de folhas de abobrinha de moita e de melancia, infetadas separadamente com as estirpes fracas e severa, e diluídos em 2 ml de tampão fosfato. O efeito da idade da planta-teste de melancia foi estudado comparando-se plantas inoculadas em três estádios de desenvolvimento, com inóculos das estirpes do PRSV-W extraídos de 12 discos foliares/2 ml de tampão. Em todos os testes de infetividade, independente da concentração do inóculo, da planta fonte do inóculo e do estádio de desenvolvimento da planta-teste, a estirpe fraca PRSV-W-3 apresentou taxas de infetividade semelhantes as das estirpes PRSV-W-1 e PRSV-W-2, chegando a 100% em alguns casos. A infetividade da estirpe severa PRSV-W-C foi de 100%. A infetividade das três estirpes fracas está mais diretamente associada à intensidade de fricção das folhas no momento da inoculação do que às outras variáveis estudadas.

scholarly journals First Report of Papaya ringspot virus on Papaya in Iran

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
R. Pourrahim ◽  
Sh. Farzadfar ◽  
A. R. Golnaraghi ◽  
N. Shahraeen
Keyword(s):  
Cucurbita Pepo ◽  
Potassium Phosphate ◽  
Ringspot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Leaf Extracts ◽  
Chenopodium Amaranticolor ◽  
First Report ◽  
Fruit Samples

Papaya, a popular fruit crop native to the American tropics, was introduced to the southern tropical provinces of Iran in the 1990s and its cultivation is widely increasing in these areas. During April 2000, severe leaf distortion and mottling were observed on papaya trees (Carica papaya) in Hormozgan Province in southern Iran. Affected trees were stunted and yielded less fruit. Samples of papaya leaf extracts (1:10 wt/vol) in 0.01 M potassium phosphate buffer (pH 7.0) were mechanically inoculated on indicator host plants, causing local lesions on Chenopodium amaranticolor and C. quinoa and chlorotic spots followed by systemic mosaic symptoms on Cucurbita pepo. Papaya ringspot virus (PRSV) was detected in the leaf samples of papaya plants and the inoculated Cucurbita pepo plants using double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with PRSV-specific antisera (polyclonal antibody AS-0086 and PV-0244, DSMZ, Braunschweig, Germany). PRSV causes one of the most destructive diseases of papaya worldwide (1). PRSV has been previously reported from Citrullus vulgaris and Cucumis melo from Iran as Watermelon mosaic virus 1 (2), but to our knowledge, this is the first report of occurrence of PRSV on papaya in Iran. References: (1) D. E. Purcifull et al. Papaya ringspot virus. No. 292. CMI/AAB, Surrey, England, 1984. (2) F. Ebrahim-Nesbat. Phytopathol. Z. 79:352, 1974.

scholarly journals Yield of varieties of Cucurbita pepo preimmunized with mild strains of Papaya ringspot virus - type W and Zucchini yellow mosaic virus

2009 ◽  
Vol 66 (3) ◽  
pp. 419-424 ◽  
Author(s):  
Estela Bonilha ◽  
Ricardo Gioria ◽  
Rômulo Fujito Kobori ◽  
Paulo Tarcísio Della Vecchia ◽  
Sônia Maria de Stefano Piedade ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Fruit Weight ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Leaf Symptoms ◽  
Severe Leaf

Papaya ringspot virus - type W (PRSV-W) and Zucchini yellow mosaic virus (ZYMV) are the most prevalent viruses in cucurbit crops in Brazil and responsible for frequent yield losses. Diseases caused by these viruses are difficult to control. The objective of this work was to evaluate the effects of mild strains PRSV-W-1 and ZYMV-M on the yield of Cucurbita pepo L. cvs. Samira, Novita Plus, AF 2847, and Yasmin, under plastic greenhouse and field conditions. Plants infected with ZYMV-M and grown in a plastic greenhouse did not exhibit typical leaf symptoms or significant alterations in quantitative and qualitative fruit yield. However, when infected with PRSV-W-1, or PRSV-W-1 + ZYMV-M, the plants exhibited severe leaf mosaic symptoms and reduced fruit quality, although there were no changes in the number and mean fruit weight harvested from these plants. When these plants were infected with PRSV-W-1 and studied simultaneously in the field and plastic greenhouse, intensification of symptoms in the fruits and leaves was more pronounced under the greenhouse conditions. Quantitative yield did not change. Environmental factors seem to influence symptoms induced by PRSV-W-1.

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