scholarly journals Assessment of light intensity and salinity regimes on the element levels of brown macroalgae, Treptacantha barbata: Application of response surface methodology (RSM)

2020 ◽  
Author(s):  
İlknur AK ◽  
Ekrem Cem ÇANKIRILIGİL ◽  
Gülen TÜRKER ◽  
Onur SEVER
Keyword(s):  
Response Surface Methodology ◽  
Light Intensity ◽  
Response Surface ◽  
Brown Macroalgae

Optimization of physical parameters for phycobiliprotein extracted from Oscillatoria agardhii and Synechococcus nidulans / Oscillatoria agardhii ve Synechococcus nidulans türlerinden fikobiliprotein ekstraksiyonu için fiziksel parametrelerin optimizasyonu

2015 ◽  
Vol 40 (6) ◽  
Author(s):  
İrem Deniz ◽  
Esra İmamoğlu ◽  
Meltem Conk Dalay
Keyword(s):  
Response Surface Methodology ◽  
Experimental Design ◽  
Light Intensity ◽  
Response Surface ◽  
Process Parameters ◽  
High Yield ◽  
Physical Parameters ◽  
Agitation Rate ◽  
Orbital Shaking ◽  
Oscillatoria Agardhii

AbstractObjective: Physical process parameters play a major role in the cultivation of cyanobacteria to provide high yield. The aim of this study was to optimize physical parameters such as light intensity and agitation rate which might affect the phycobiliprotein formations for cyanobacterial strains of Oscillatoria agardhii and Synechococcus nidulans using response surface methodology.Methods: The cyanobacterial strains were cultured in 250 mL flasks containing 100 mL of EM medium in orbital shaking incubator under the temperature of 22±2°C at different light intensities and agitation rates for 10 days. The experimental design was carried out using 2Results: The optimization solution of O. agardhii (approximately at 156 rpm under the light intensity of 65 μmol photons mConclusion: High agitation rate stimulated the faster growth than increased the light intensity for the growths of cyanobacterial strains.

scholarly journals Optimization of Chlorella Culture Conditions with Response Surface Methodology to Increase Biomass

2021 ◽  
Vol 20 (5) ◽  
Author(s):  
R. Kanimozhi ◽  
D. Arvind Prasath ◽  
R. Dhandapani ◽  
Santhosh Sigamani
Keyword(s):  
Response Surface Methodology ◽  
Light Intensity ◽  
Response Surface ◽  
Biomass Yield ◽  
Culture Conditions ◽  
Optimal Conditions ◽  
Optimum Conditions ◽  
Accession Number ◽  
Microalgal Biomass ◽  
Chlorella Sp

Microalgae is gaining popularity as a major ingredient in nutrition supplements. To mass cultivate, it is imperative to improve the biomass yield hence optimization of cultures conditions becomes paramount. In this work, an attempt has been made to optimize the microalgal production using response surface methodology (RSM) and validate further the optimized parameters. The optimum conditions for the cultivation of Chlorella sp. KPU016 under optimized nutrient conditions were pH 8.2, the light intensity of 3100 lx, glycerol 1.44 g.L-1 (under pre-set conditions of 12 h lighting, the temperature at 27±1°C. With these RSM-driven optimum conditions, the yield of microalgal biomass achieved was 282.50 mg.L-1. For larger-scale microalgal harvesting, the validated optimal conditions can be inferred as the best for enhanced microalgal production. The isolate was partially sequenced and submitted to the NCBI database and the GenBank accession number is MZ348364.

scholarly journals Ultrasound-Assisted Extraction using Response Surface Methodology for Extracting Flavonoids from Padina australis

2021 ◽  
Vol 50 (5) ◽  
pp. 1321-1328
Author(s):  
Bina Lohita Sari ◽  
Tri Saptari Haryani ◽  
Triastinurmiatiningsih Triastinurmiatiningsih ◽  
Dinda Rizki Amalia
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Total Flavonoid ◽  
Process Time ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ultrasound Assisted Extraction ◽  
Brown Macroalgae ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Seaweed or sea macroalgae are rich in potential compounds which can be used for the treatment of disease. Padina australis is one of the important brown macroalgae classes (Phaeophyes). One of the bioactive compounds of P. australis is a phenolic compound and its derivatives (flavonoid). In this research, P. australis was collected from Bayah Beach, Banten, Indonesia. For the extraction flavonoids from P. australis, ultrasound-assisted extraction (UAE) was employed. In this study, a three-level Box-Behnken design (BBD) and the response surface methodology (RSM) were employed to obtain the optimal combination of extraction conditions. The effects of several independent variables including temperature (30, 50, 70 ℃), extraction time (20, 40, 60 min) and ethanol concentration (30, 50, 70%) were investigated. The result showed that RSM was an accurate and reliable method in predicting the total flavonoid content with R2 value of 0.9935. The optimal UAE conditions for the highest yield of total flavonoid content were 49.70 ℃ in temperature, process time under 44.03 min, and 47.80% ethanol with 0.2162% total flavonoid content. Under the above conditions, the experimental value of total flavonoid content was 0.2144+ 0.0035%. The predicted and experimental values for total flavonoid from brown algae P. australis were not significant differences, it indicating that the developed models are accurate. Therefore, UAE using RSM is effective for the extraction of flavonoid from P. australis.

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