scholarly journals Insecticidal activity of 2-tridecanone against the cowpea weevil Callosobruchus maculatus (Coleoptera: Bruchidae)

2007 ◽  
Vol 79 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Yussef F.B. Braga ◽  
Thalles B. Grangeiro ◽  
Eder A. Freire ◽  
Helano L. Lopes ◽  
José N. S. Bezerra ◽  
...  

The effect of 2-tridecanone vapor on the cowpea weevil (Callosobruchus maculatus) development was determined. Seeds of cowpea were infested with adults and exposed to different doses of 2-tridecanone isolated from Pilocarpus microphyllus Stapf ex Holm, a plant species native from northeastern Brazil. The pure monoterpene was evaluated both undiluted as well as in the dilutions 1:10, 1:100 and 1:1,000 (v/v). The following parameters of the cowpea weevil life cycle were analyzed in response to decreasing doses of 2-tridecanone: number of eggs laid, percentage of egg hatching on seeds, percentage of adult emergence, adult weight at emergence, mean developmental time and number of adults emerged. Vapor of 2-tridecanone caused a significant (P < 0.05) reduction in the number of eggs laid, in the percentage of eggs hatched and in the number of emerged adults in infested seeds. The fumigant insecticidal effect of 2-tridecanone was mainly due to its ovicidal activity.

1970 ◽  
Vol 15 ◽  
pp. 83-88 ◽  
Author(s):  
M Saiful Islam ◽  
Fauzia Akhter ◽  
Rezina Laz ◽  
Selina Parween

Seeds of black grams, lentils, Bengal grams and green peas were soaked separately in aqueous solutions of Triflumuron at doses of 0.0 (control), 0.5, 1.0, 1.5 and 2.0 ppm. Three day -old adults of Callosobruchus maculatus (F.) (Coleoptera: Bruchidae) were allowed to oviposit on air-dried, treated or untreated pulses of each type and dose in ‘no-choice' and ‘free-choice' tests. Maximum oviposition occurred in Bengal grams (61.00? 0.25) and green peas (33.67? 0.54) in ‘no-choice' and ‘free-choice' tests, respectively. The beetles avoided egg laying on lentils. Maximum egg-hatching occurred on black grams (>90%) in both tests. Minimum developmental time was required in the Bengal grams (30? 0.5 days in ‘no-choice' test and 31.33? 1.23 days in ‘free-choice' test). No adults emerged from green peas, while 54.79% and 86.15% emergence were recorded from untreated black grams in ‘no-choice' and ‘free-choice' tests, respectively. Triflumuron reduced egg-laying significantly in green peas, where hatchability also reduced significantly to 35.99% at 2 ppm in ‘no-choice' test. Percentage of hatching decreased in all pulses with the increasing doses of triflumuron. No adults emerged from the treated green peas in any test, and at 2 ppm the adult emergence declined to < 50% in all pulses. Implications of these results are further discussed.  Key words: Callosobruchus maculatus, Triflumuron, seed protectant, fecundity, hatchability, developmental period, adult emergence   doi: 10.3329/jbs.v15i0.2207 J. bio-sci. 15: 83-88, 2007


2019 ◽  
Vol 32 (4) ◽  
pp. 1117-1124 ◽  
Author(s):  
MARIA JOSÉ ARAÚJO WANDERLEY ◽  
NIVÂNIA PEREIRA DA COSTA ◽  
THATIANA MARIA BORGES SILVA ◽  
GEORGE RODRIGO BELTRÃO DA CRUZ ◽  
THIAGO DE SOUSA MELO

ABSTRACT Callosobruchus maculatus, commonly known as cowpea weevil, is the main pest affecting stored cowpea Vigna unguiculata. The damage resulting from the penetration and feeding of cowpea weevil larvae within the grains or seeds of V. unguiculata leads to weight loss, reduction of germination power and nutritional value and commercial depreciation of this plant. The objective of this work was to evaluate the insecticidal activity and repellency of vegetable oils extracted from fennel, citronella, neem and 'moringa' against C. maculatus adults. To evaluate the insecticidal activity, we placed 20 adult insects in plastic containers lined with filter paper impregnated with different doses (0.0 x 106, 5.0 x 106, 1.0 x 105, 1.5 x 105, 2.0 x 105, 3.05, 4.0 x 105 and 5.0 x 105 L) of each vegetable oil. The number of insects was analyzed 24 hours later. To evaluate the repellency activity, we used an arena formed by five circular plastic containers with the central container interconnected symmetrically to the others by plastic tubes arranged diagonally. Samples of 30 g of cowpea were impregnated with different doses (5.0 x 106, 1.0 x 105, 1.5 x 105 and 2.0 x 105 L) of each vegetable oil. We released 50 adults of C. maculatus in the central container and counted the number of insects per container 24 hours later. Citronella and fennel oils at a dose of 3.0 x 105 L demonstrated a higher insecticidal effect on C. maculatus. However, citronella oil presented the best repellent action.


Author(s):  
S. Ragul ◽  
N. Manivannan ◽  
K. Iyanar ◽  
N. Ganapathy ◽  
G. Karthikeyan

Background: Blackgram [Vigna mungo (L.) Hepper] is a rich source of protein. It is one of the major crops essentially involved in daily human diets. However, storage pest bruchine [Callosobruchus maculatus (F.)] is a major production constraint for legumes. A research was formulated to assess the bruchine resistance in 20 blackgram genotypes along with the biochemical analysis to find out the active biochemical components responsible for the resistance activity.Methods: The experiment was carried out during August- October, 2019 at Entomology Laboratory, National Pulses Research Center, Vamban, India. The experimental material comprised of 20 blackgram genotypes which were screened for bruchine resistance. Further, confirmatory trial was conducted with selected resistant entries and highly susceptible entries during October- December, 2019. Both experiments were carried out in completely randomized design and replicated three times. GC-MS analysis on the resistant and susceptible entries were performed to ascertain the active biochemical components conferring resistance.Result: Among the genotypes, TU 68 had comparatively late developmental time (days), less number of adult emergence, higher mean developmental period (days), less susceptibility index, less seed damage (%) and less seed weight loss (%). Genotype TU 68 was found to be resistant in the confirmatory trial also. Less number of adult emergence and higher mean developmental period indicated the delayed developmental period which is a mechanism of bruchine resistance. GC-MS analysis on resistant (TU 68) and susceptible (MDU 1) genotypes indicated the presence of active biochemical compounds with insectifuge activity in TU 68. Hence, TU 68 could be utilized in the hybridization programmeas donor for bruchine resistance.


2020 ◽  
Vol 2 (1) ◽  
pp. p128
Author(s):  
Mohammed Suleiman ◽  
Fatima M. Haruna

Experiments were conducted to assess the protectant ability of cow dung ash (CDA) to cowpea seeds against Callosobruchus maculatus F. under laboratory conditions of 50-65% R.H and 28-32oC. The CDA at different rates of 0.625, 1.25, 2.50, 5.00, 10.00% (w/w) incorporated with cypermethrin powder at 0.625% were applied to 20 g of cowpea seeds in petri dishes. None of the ashes or cypermethrin was added to the control. Ten beetles were introduced into each of the petri dishes and covered with muslin cloth. The experiment was arranged in a completely randomized design (CRD) and replicated three times. Effects of the treatments on adult mortalities as well as median lethal dose (LD50) of CDA against C. maculatus were determined. Adult emergence and weight losses in seeds treated with CDA at different doses were assessed. Findings of the study revealed that the adult mortality of C. maculatus among different CDA treatments differed significantly (p < 0.05) and ranged from 13.33 to 100.00% 72 hours after treatment (HAT). Results of the study also showed that LD50 of CDA against C. maculatus at 24 hours after treatment (HAT) was 5.51 % (w/w). No adults emerged from the treated seeds and weight losses were observed in those treated with the lowest dose and the control only. Therefore, CDA could be utilized to protect cowpea seeds from C. maculatus infestations during storage.


1970 ◽  
Vol 5 ◽  
pp. 17-22 ◽  
Author(s):  
M Saiful Islam ◽  
Sharmin Mustari

Using 254nm UV radiations of exposure periods from 2 to 16 min against adults. Time mortality response of the cowpea weevil, Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae), adults and alterations in vital reproductive attributes as fecundity, immature duration, adult emergence and adult longevity of the beetles from parental through F1 generation have been estimated. Time mortality response of the adults of the cowpea weevil Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae), and alternations in vital reproductive attributes is fecundity, immature duration, adult emergence and longevity from parental through F1 generation using 254nm UV radiations of response periods from 2-16min have been estimated. Results show that an exposure time to kill 50% of the adults is around 20 min (LT50 = 19.99min), and irradiation significantly reduced egg-laying (P<0.001), lengthened immature durations (i.e. larval and pupal developmental periods; P<0.05), decreased adult emergences as well as longevity in both sexes (P<0.001) in the parental generation. The effects of the UV-rays on F1 progenies, however, were less pronounced than that expressed in the parental generation, but the immature duration was significantly increased (P<0.01) but the longevity in both sexes was significantly reduced (P<0.001). Relevance of this study in relation to prospective phytosanitary treatments of the stored pulses with UV-rays has been discussed. Key words: UV irradiation; Callosobruchus maculatus; time-mortality response; reproductive attributes; stored pulses DOI: 10.3329/jles.v5i0.7343 J. Life Earth Sci., Vol. 5: 17-22, 2010


2001 ◽  
Vol 81 (1) ◽  
pp. 173-177 ◽  
Author(s):  
Sékou Moussa Kéïta ◽  
Charles Vincent ◽  
Jean-Pierre Schmidt ◽  
John Thor Arnason

Analysis of Thuja occidentalis L. essential oil used for insect fumigation by phase gas chromatography revealed the presence of 22 compounds including α-thujone (49.64%), fenchone (14.06%), and β-thujone (8.98%). Fumigation of adult bruchids with the oil alone resulted in lethal doses (LD50) of 1.1, 0.7, 0.5 and 0.2 μL/insect after 3, 6, 9 and 12 h, respectively. When insects were treated with aromatized powder, significant differences were also found between treatments and control. Application of 100 mg of powder aromatized at 3 μL essential oil g–1 on bruchid pairs lead to 95% mortality of females and 100% of males with 0% of mortality in the control after 6 h exposure. Five days after their deposit, egg hatching was 1.2% (treated with kaolin powder aromatized with T. occidentalis essential oil), 41% (with kaolin alone) and 44% of eggs (control without kaolin). In the same experiment, adult emergence of 80% (in treatments with kaolin alone), 100% in control (without kaolin) and 0% (with kaolin aromatized with T. occidentalis essential oil) were recorded 30 d after treatment. Germination of cowpea seeds was not significantly affected by the treatments. Five days after sowing, germination was 88, 97 and 97%, respectively, when cowpea grains were treated and exposed, treated and unexposed, untreated and unexposed, respectively, while those untreated and exposed had 15% germination. Key words: Thuja occidentalis, Callosobruchus maculatus, essential oil, kaolin, fumigation, ethnobotany.


2015 ◽  
Vol 148 (1) ◽  
pp. 102-111 ◽  
Author(s):  
Poulami Adhikary ◽  
Ujjwal Malik ◽  
Anandamay Barik

AbstractThe effect of four varieties of Lathyrus sativus Linnaeus (Fabaceae) seeds (Bio L 212 Ratan, Nirmal B-1, WBK-14-7, and WBK-13-1) on the bionomics of Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae: Bruchinae) was studied under laboratory conditions. Total larval developmental time was longer on WBK-13-1 than Bio L 212 Ratan. Adult emergence was highest on Bio L 212 Ratan followed by Nirmal B-1, WBK-14-7, and WBK-13-1. The development and fecundity were related with nutrient and antinutritional factors of khesari seeds. Total carbohydrates and proteins were highest in Bio L 212 Ratan and Nirmal B-1 while lipids and nitrogen were highest in Bio L 212 Ratan and least in WBK-13-1. Amino acids were higher in Nirmal B-1 and WBK-13-1. Phenols were greatest in Nirmal B-1 and least in Bio L 212 Ratan and WBK-14-7. β-ODAP was higher in Bio L 212 Ratan and Nirmal B-1. The lower levels of carbohydrates, proteins, lipids, nitrogen, water content, and higher trypsin inhibitor activity of WBK-14-7 and WBK-13-1 may explain the higher developmental time and lower fecundity of C. maculatus on these varieties. These results suggest that infestations of C. maculatus may be easier to manage on WBK-14-7 and WBK-13-1 than on the other varieties of khesari seeds.


2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Olufunmilayo E. Ajayi ◽  
Arthur G. Appel ◽  
Henry Y. Fadamiro

The fumigant toxicity of eight essential oil components, 1-8-cineole, carvacrol, eugenol, (−)-menthone, (−)-linalool, S-(−)-limonene, (−)-β-pinene, and (+)-α-pinene, was tested against the cowpea weevil, Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae), at 0.25–60 µL/L air doses. 1-8-Cineole, carvacrol, and eugenol caused complete adult mortality at 10 µL/L air 24 h after treatment. 1-8-Cineole and carvacrol were the most toxic with LD50 values of 0.24 and 0.6 µL/L air at 24 h, respectively. (−)-β-Pinene and (+)-α-pinene were the least toxic with LD50 values of 31 and 31.4 µL/L air at 24 h, respectively. Toxicity was negatively correlated with vapor pressure. 1-8-Cineole and carvacrol caused 100% oviposition deterrence at all doses tested. Eugenol and (−)-menthone completely inhibited adult emergence. S-(−)-Limonene, (−)-β-pinene, and (+)-α-pinene were not effective at preventing oviposition or adult emergence, suggesting that a lethal dose of the three oil components would be necessary to control C. maculatus infestations.


2020 ◽  
Vol 145 (1) ◽  
Author(s):  
Fevzi Uçkan ◽  
Rabia Özbek ◽  
Ekrem Ergin

The effects of indol-3-acetic acid (IAA) were investigated on biological parameters of Galleria mellonella (Linnaeus, 1758) (Lepidoptera: Pyralidae) and its endoparasitoid Pimpla turionellae (Linnaeus 1758) (Hymenoptera: Ichneumonidae) reared on hosts treated with 50 to 10,000 ppm of IAA in their diet. Percent fecundity of first generation of G. mellonella females increased by 12% at 50 ppm whereas the most effective decrease, by 33%, was observed at 5,000 ppm. Percent fertility decreased by 20% at 1,000 ppm. All treatments displayed ±5% variations when compared to controls except for a 20% increase in corrected percent sterility at 1,000 ppm. The results did not reveal any considerable effect of IAA on egg hatching, larval and pupal developmental, or adult emergence times. The most striking effect observed was a decline in second generation pupal developmental time by 47% at 1,000 and 10,000 ppm. No dose-wise alterations were observed in adult longevity, weight, size and female sex ratio of G. mellonella. Treatment with IAA caused a slight prolongation in adult emergence and decrease in longevity of P. turionellae reared on hosts; longevity of wasps declined by 27% at higher doses. Neither wasp size nor weight displayed significant changes upon IAA treatment.


Author(s):  
Olusola Michael Obembe ◽  
Joshua Kayode

A laboratory study was conducted to investigate the efficacy of powder and extract of C. alata on Callosobruchus maculatus Fab. damage in stored cowpea (Vigna unguiculata L.) seeds. The powder and the extract were evaluated on C. maculatus for mortality, oviposition and adult emergence effects. The long-term storage of the treated seeds was also investigated. All treatments recorded higher significant (p < 0.05) mortality than the experimental control. Highest mortality of 100 % was recorded in seeds treated with C. alata extract at the dosage level of 4.0 % v/w while highest mortality of 80.30 % was also recorded in seed treated with 4.0 g of C. alata powder. The powder and the extract significantly reduced oviposition and adult emergence in the treated seed. Adult emergence was totally suppressed in seeds treated with 3.0 % v/w and 4.0 % v/w. The powder and the extract also reduced infestation and subsequence damage of the treated seeds. No damage was recorded in seeds treated with 3.0 % v/w and 4.0 % v/w of the extract. The result obtained revealed that extract and powder of C. alata were effective in controlling C. maculatus and could be recommended as an alternative to synthetic insecticides.


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