scholarly journals Plague surveillance in Brazil: 1983 - 1992

1995 ◽  
Vol 37 (6) ◽  
pp. 511-516 ◽  
Author(s):  
Alzira Maria Paiva de Almeida ◽  
Nilma Cintra Leal ◽  
Francisco Gomes de Carvalho ◽  
José Dantas Sobrinho ◽  
Célio Rodrigues de Almeida
Keyword(s):  
Rural Areas ◽  
Serological Testing ◽  
Passive Hemagglutination ◽  
Specific Antibodies ◽  
Natural Foci ◽  
Human Sera ◽  
Nationwide Surveillance ◽  
Inhibition Control ◽  
Domestic Carnivores ◽  
Sera Samples

Plague caused by Yersinia pestis, has persisted in Brazil in several natural foci spread throughout rural areas in the States of Ceara, Paraiba, Pernambuco, Piaui, Rio Grande do Norte, Alagoas, Bahia, Minas Gerais and Rio de Janeiro. Nationwide surveillance of plague in Brazil based on serological testing started in 1983. We now present an update report of the examinations carried out in our laboratory from 1983 to 1992. The passive hemagglutination test for antibodies against fraction 1A antigen of Y. pestis and the passive hemagglutination inhibition control were employed for testing a total of 220,769 sera. Samples analyzed included 2,856 sera from clinically diagnosed plague cases or suspects, 49,848 sera from rodents of 24 species and 2 species of small wild carnivores (marsupials), 122,890 sera from dogs, and 45,175 sera from cats. Specific antibodies were found in 92 (3.22%) human sera; 143 (0.29%) sera from rodents of 8 species and from the two species of marsupials, 1,105 (0.90%) sera from dogs and 290 (0.64%) sera from cats. The presence of significant levels of specific anti-F1A antibodies among rodents and wild or domestic carnivores (dogs and cats) indicates that all the Brazilian plague foci remain active in spite of the absence of human cases in some of them.

scholarly journals A serological survey of canine leptospirosis in the city of Belgrade, Serbia

2022 ◽  
pp. 1-1
Author(s):  
Dragica Vojinovic ◽  
Jadranka Zutic ◽  
Ana Vasic ◽  
Slobodan Stanojevic ◽  
Ljiljana Spalevic ◽  
...  
Keyword(s):  
Urban Areas ◽  
Animal Species ◽  
Agglutination Test ◽  
Serological Testing ◽  
Serological Survey ◽  
Specific Antibodies ◽  
Leptospira Spp ◽  
Antibody Titres ◽  
The City ◽  
Sera Samples

Canine leptospirosis is a zoonosis caused by bacteria belonging to the genus Leptospira. Dogs are one of the animal species involved in the cycle of preservation and transmission of leptospirosis in urban areas. Serological testing for the presence of specific antibodies against Leptospira spp. in dogs was continuously performed between 2010 and 2020 in the city of Belgrade. At the request of the owners themselves, other veterinary laboratories or laboratory clinics, 179 blood sera from 179 dogs were examined in the Laboratory for Immunology, Scientific Institute of Veterinary Medicine of Serbia. Blood sera samples from dogs were examined using the standard microscopic agglutination test (MAT) for the presence of specific antibodies against seven different serovars of Leptospira: Pomona, Icterohaemorrhagiae, Grippotyphosa, Sejroe, Canicola, Bataviae, and Australis. The number of seropositive dogs was 17/179 (9.5%). Among all examined sera, the highest titre of seropositive samples was to serovar Icterohaemorrhagiae (10/17, 58.8%), followed by Pomona (4/17, 23.5%), and serovar Canicola (3/17, 17.6%). Specific antibodies for serovars Grippotyphosa, Sejroe, Bataviae and Australis were not detected in any of the dog sera. Cross-reaction (the presence of two or three titres with different values where one of them was higher than others) between different serovars was diagnosed in a low number of sera (n=4), with the following serovars: Icterohaemorrhagiae and Pomona (n=3) and Pomona and Canicola (n=1). The confirmed specific antibody titres for Leptospira spp. were between 1:100 to 1:3000 (5 sera had titres of 1:100, 7 had titres of 1:300, 4 had titres of 1:1000, and 1 serum had a titre 1:3000). Monitoring canine leptospirosis is a useful tool in preventing leptospirosis in Belgrade.

scholarly journals REGIONAL FEATURES OF THE FORMATION OF THE NOSOLOGICAL PROFILE OF INFECTIOUS ANIMAL DISEASES IN THE REPUBLIC OF BURYATIA

2021 ◽  
pp. 73-81
Author(s):  
O. B. Badmaeva
Keyword(s):  
Clinical Signs ◽  
Buffer Zone ◽  
Farm Animals ◽  
Wild Animals ◽  
Natural Focus ◽  
Regional Features ◽  
Focal Infection ◽  
Natural Foci ◽  
The Republic ◽  
Domestic Carnivores

On the territory of the Republic of Buryatia, the epizootological profile is formed by 8 nosological forms of infectious pathology of farm animals. The dominant epizootic significance is rabies, leptospirosis, brucellosis. Rabies was registered in 8 (38.1 %) rural administrative districts of the republic and in the urban district of Ulan-Ude. In the total number of cases of rabies, the disease of farm animals occupies 48.3%, domestic carnivores-3.9, and in 47.6% of cases, epizootic foci began among wild animals. The main reservoir of the rabies virus is the wild fox: 46.2 % in the total number of cases and 97.1 % - in autochthonous epizootic foci. Leptospirosis in the conditions of Buryatia is an indigenous natural focal infection with a pronounced indicator of epizootic manifestation in the form of infection of animals without clinical signs. It is registered in 11 (52.4 %) rural administrative districts of the republic in 1.5 % of cattle and 0.6% of horses. The unfavorable situation with bovine brucellosis persisted from 2009 to 2018, 26 unfavorable points were registered. In the Jida district, 5 km from the state border with Mongolia, brucellosis was first registered in a dog. In this area, the corridor of migration of wild animals across the border passes, which confirms the assumption of the existence of natural foci of brucellosis in the transboundary territories of Russia and Mongolia and the introduction of the pathogen from the natural focus to the territory of the buffer zone farms.

Carbohydrate-Specific Antibodies in Normal Human Sera

Vox Sanguinis ◽  
1984 ◽  
Vol 47 (2) ◽  
pp. 146-156
Author(s):  
Parviz Lalezari ◽  
An Fu Jiang
Keyword(s):  
Specific Antibodies ◽  
Human Sera ◽  
Normal Human

scholarly journals THE IMMUNE RESPONSE TO A HYBRID PROTEIN MOLECULE

1967 ◽  
Vol 126 (4) ◽  
pp. 581-606 ◽  
Author(s):  
K. Rajewsky ◽  
Elke Rottländer ◽  
G. Peltre ◽  
Brigitte Müller
Keyword(s):  
Immune Response ◽  
Dose Range ◽  
Protein Molecule ◽  
The Other ◽  
Hybrid Protein ◽  
Passive Hemagglutination ◽  
Specific Antibodies ◽  
Hybrid Enzyme ◽  
Subsequent Response ◽  
Subunit B

Upon immunization with LDH-III (subunit composition AABB) rabbits produce anti-A and anti-B antibodies in comparable amounts. These antibodies fit equally well to the hybrid enzyme and to LDH-V (AAAA) or LDH-I (BBBB) respectively, as tested by passive hemagglutination inhibition. No antibodies reacting with both LDH-I and LDH-V were detected. A minority of hybrid-specific antibodies was, however, present in the sera. Animals primed with LDH-III respond regularly to a boosting injection of LDH-V with the production of large amounts of anti-A (but not anti-B) antibodies. A similar injection of LDH-I stimulates (if it has any effect at all) the production of anti-B antibodies only. Stimulation with one of the pure types does not impair a subsequent response to the other. The majority of the animals primed with LDH-III responded not at all or weakly to a boosting injection of LDH-I, though antibodies to LDH-I were present in the sera at the time of stimulation. This effect can hardly be explained on the basis of serological sepcificity. Hyporesponsiveness to LDH-III can be induced by injection of LDH-V into the newborn. Both anti-A and anti-B titers are equally depressed. Within the dose range tested, LDH-I does not exert any tolerogenic action with respect to LDH-III. The carrier property of subunit A is evident in the induction of both immunity and tolerance to LDH-III. The early phase of the immune response to the hybrid enzyme may be carrier-specific, and receptors for the haptenic subunit B may not exist at that stage.

Determination of Trace Elements in Serum Using Inductively Coupled Plasma Atomic Spectroscopy with Hydride Generation and Chelating Resin Preconcentration

1984 ◽  
Vol 38 (5) ◽  
pp. 635-644 ◽  
Author(s):  
Zhuang Mianzhi ◽  
Ramon M. Barnes
Keyword(s):  
Trace Elements ◽  
Inductively Coupled Plasma ◽  
Hydride Generation ◽  
Atomic Spectroscopy ◽  
Chelating Resin ◽  
Plasma Emission ◽  
Inductively Coupled ◽  
Human Sera ◽  
Sera Samples

A procedure is described for the inductively coupled plasma emission spectroscopic determination of Cd, Co, Cu, Fe, Mo, V, and Zn in < 1 mL of serum after digestion in nitric and perchloric acids and preconcentration with a poly(dithiocarbamate) resin Recoveries are 91 to 106% The determination of As and Se in serum by hydride generation and ICP-AES is performed after the interference of Cu on Se hydride generation was reduced by complexation of the Cu with poly(acrylamidoxime) resin Verification with NBS-SRMs as well as with human sera samples is reported

scholarly journals Prefusion F–specific antibodies determine the magnitude of RSV neutralizing activity in human sera

2015 ◽  
Vol 7 (309) ◽  
pp. 309ra162-309ra162 ◽  
Author(s):  
Joan O. Ngwuta ◽  
Man Chen ◽  
Kayvon Modjarrad ◽  
M. Gordon Joyce ◽  
Masaru Kanekiyo ◽  
...  
Keyword(s):  
Specific Antibodies ◽  
Neutralizing Activity ◽  
Human Sera

scholarly journals Radioimmunoassay for measuring antibodies specific for group B streptococcal types Ia, Ib, Ic, II, and III

1976 ◽  
Vol 3 (5) ◽  
pp. 480-485
Author(s):  
H W Wilkinson ◽  
W L Jones
Keyword(s):  
Carbohydrate Antigens ◽  
Specific Antibodies ◽  
Type Antigen ◽  
Human Sera ◽  
Group B ◽  
Primary Type ◽  
Antigen Antibody ◽  
Antibody Interactions

The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing human sera for group B type-specific antibodies, since it requires very small quantities of antigens and measures primary type antigen-antibody interactions.

Sign in / Sign up

Export Citation Format

Share Document