Amplification of the flgE gene provides evidence for the existence of a Brazilian borreliosis

INTRODUCTION: The symptoms of Brazilian borreliosis resemble the clinical manifestations of Lyme disease (LD). However, there are differences between the two in terms of epidemiological and laboratory findings. Primers usually employed to diagnose LD have failed to detect Borrelia strains in Brazil. OBJECTIVE: We aimed to identify the Brazilian Borrelia using a conserved gene that synthesizes the flagellar hook (flgE) of Borrelia burgdorferi sensu lato. METHOD: Three patients presenting with erythema migrans and positive epidemiological histories were recruited for the study. Blood samples were collected, and the DNA was extracted by commercial kits. RESULTS: The gene flgE was amplified from DNA of all selected patients. Upon sequencing, these positive samples revealed 99% homology to B. burgdorferi flgE. CONCLUSION: These results support the existence of borreliosis in Brazil. However, it is unclear whether this borreliosis is caused by a genetically modified B. burgdorferi sensu stricto or by a new species of Borrelia spp.

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Laemophloeus souzalimai, a new species of lined flat bark beetle (Coleoptera, Cucujoidea, Laemophloeidae) from Southeast Brazil

Zootaxa ◽

10.11646/zootaxa.5072.3.4 ◽

2021 ◽

Vol 5072 (3) ◽

pp. 278-284

Author(s):

MATHEUS BENTO

Keyword(s):

New Species ◽

Bark Beetle ◽

New World ◽

New Records ◽

Sensu Stricto ◽

World Species ◽

Paulo State ◽

Southeast Brazil ◽

Male Head ◽

A New Species

Distinctive, remarkable specimens from Bauru, São Paulo state, Brazil are recognized as a new species of the genus Laemophloeus Dejean (sensu stricto), which is described and illustrated in this paper. Laemophloeus souzalimai sp. nov. is most similar to L. germaini Grouvelle and can be readily distinguished from all New World species of the genus by the dorsal color testaceous, without elytral maculae, antennal club of 6 antennomeres, genal processes anteriorly projected, male head with somewhat pedunculated eyes, and frons with a broad, V-shaped impressed line. Additionally, the preexisting key to Laemophloeus species with antennal club of 6 or more antennomeres is modified to include the new species, and new records for five Brazilian species of the genus are provided.

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Inocybe cervenianensis (Agaricales, Inocybaceae), a new species in the I. flavoalbida clade from Italy

Phytotaxa ◽

10.11646/phytotaxa.484.2.7 ◽

2021 ◽

Vol 484 (2) ◽

pp. 227-236

Author(s):

FRANCESCO DOVANA ◽

ENRICO BIZIO ◽

MATTEO GARBELOTTO ◽

GIULIANO FERISIN

Keyword(s):

New Species ◽

Natural Habitat ◽

Molecular Data ◽

Sensu Stricto ◽

New Taxon ◽

Microscopic Features ◽

A New Species

The authors describe the macro- and microscopic features of Inocybe cervenianensis, a new taxon belonging to the genus Inocybe sensu stricto. The description is illustrated with photographs of the basidiomes in their natural habitat and photographs of the main microscopic features. Molecular data (nrITS and RPB2) support the recognition of this new species.

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A new species of Pseudancistrus from the Rio Caroni Venezuela (Siluriformes: Loricariidae)

Zootaxa ◽

10.11646/zootaxa.1731.1.3 ◽

2008 ◽

Vol 1731 (1) ◽

pp. 33 ◽

Cited By ~ 6

Author(s):

JONATHAN W. ARMBRUSTER ◽

DONALD C. TAPHORN

Keyword(s):

New Species ◽

Conservation Status ◽

Color Pattern ◽

Sensu Stricto ◽

Dorsal Plate ◽

Stream Capture ◽

Especie Nueva ◽

Del Rio ◽

Relationship Of ◽

A New Species

Pseudancistrus reus is a new species from the Río Caroní (Río Orinoco drainage) of Venezuela known from two individuals. It differs from all other Pseudancistrus by having a color pattern consisting of alternating dark and light bars. In addition, it differs from all except P. genisetiger and P. papariae by having an incomplete mid-dorsal plate row and from P. genisetiger and P. papariae by having 18 contiguous mid-dorsal plates vs. 14 plates, a plateless break and then two more plates at the end of the caudal peduncle. The type locality of P. reus was submerged by the construction of the Caruachi dam, and is also the only known locality of the gymnotiform Sternarchorhynchus gnomus, making it imperative that the conservation status of these and other potential Caroní endemics be assessed. Pseudancistrus reus is the first species of Pseudancistrus sensu stricto from the Orinoco. Although the relationship of the species to other Pseudancistrus is unknown, P. reus may have gained access to the Orinoco either via stream capture between the Caroní and the Rio Uraricoera (Rio Branco – Rio Negro drainage) or via stream capture between the Caroní and either the Cuyuní or Mazaruni Rivers (Essequibo River drainage).key word: Siluriformes, Loricariidae, taxonomySe describe como especie nueva Pseudancistrus reus del río Caroní (cuenca del río Orinoco) en Venezuela, basada en dos ejemplares. Difiere de todas los demás Pseudancistrus en tener un patrón de pigmentación que consiste de barras claras alternando con oscuras. Además, difiere de todas los demás, menos P. genisetiger y P. papariae en tener la fila medio-dorsal de placas incompleta, y de P. genisetiger y P. papariae difiere en tener 18 placas medio-dorsales contiguas vs. 14 placas seguidas por una zona sin placas y luego dos placas más en el pedúnculo caudal). La localidad típica de P. reus fue sumergida por las aguas del embalse Caruachi. Ese sitio es también la localidad típica de Sternarchorhynchus gnomus, haciéndolo imprescindible evaluar el estado de conservación de estas dos especies más las otras endémicas del bajo Caroní. Pseudancistrus reus es la primera especies de Pseudancistrus sensu stricto de la cuenca del río Orinoco. Aunque desconocemos las relaciones con otras Pseudancistrus, P. reus puede haber ganado acceso al Caroní vía la captura de caños en las cabeceras del río Uraricoera-Branco-Negro o del río Cuyuní o Mazaruni, afluentes del río Essequibo.

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A new species of Tenuipalpus sensu stricto (Acari: Tenuipalpidae) from Brazil, with ontogeny and a key to the known species

Zootaxa ◽

10.11646/zootaxa.4088.3.3 ◽

2016 ◽

Vol 4088 (3) ◽

pp. 355 ◽

Cited By ~ 2

Author(s):

ELIZEU B. CASTRO ◽

REINALDO J.F. FERES ◽

RONALD OCHOA ◽

GARY R. BAUCHAN

Keyword(s):

New Species ◽

Sensu Stricto ◽

A New Species

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Cortinarius khinganensis (Agaricales), a new species of section Illumini from Northeast China

Phytotaxa ◽

10.11646/phytotaxa.500.1.1 ◽

2021 ◽

Vol 500 (1) ◽

pp. 1-10

Author(s):

MENG-LE XIE ◽

TIE-ZHENG WEI ◽

BÁLINT DIMA ◽

YONG-PING FU ◽

RUI-QING JI ◽

...

Keyword(s):

New Species ◽

Phylogenetic Relationships ◽

Northeast China ◽

Phylogenetic Analyses ◽

Morphological Characteristics ◽

Sensu Stricto ◽

Similar Species ◽

Molecular Phylogenetic ◽

A New Species

This study presents one telamonioid species new to science based on morphological characteristics and molecular phylogenetic analyses. Cortinarius khinganensis was collected from the Greater Khingan Mountains, Northeast China and it is characterized by hygrophanous, vivid brownish red and striate pileus, white universal veil, and subglobose spores. According to phylogenetic analyses results, C. khinganensis belongs to the section Illumini, which is a lineage distantly related from subgenus Telamonia sensu stricto. Detailed descriptions of the new species and the comparisons with morphologically similar species are provided. The phylogenetic relationships within the section Illumini are also discussed.

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Diversity ofRhabdochona mexicana(Nematoda: Rhabdochonidae), a parasite ofAstyanaxspp. (Characidae) in Mexico and Guatemala, using mitochondrial and nuclear genes, with the description of a new species

Journal of Helminthology ◽

10.1017/s0022149x19000014 ◽

2019 ◽

Vol 94 ◽

Cited By ~ 2

Author(s):

A. Santacruz ◽

C.P. Ornelas-García ◽

G. Pérez-Ponce de León

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Sensu Stricto ◽

Parasitic Nematodes ◽

Molecular Tools ◽

Genetic Lineages ◽

Candidate Species ◽

High Species Richness ◽

Biogeographical Regions ◽

A New Species

AbstractAmong fish parasitic nematodesRhabdochonais one of the most speciose genera, withc.100 species. Twelve congeneric species occur in Mexican freshwater fishes, in a region located between the Nearctic and Neotropical biogeographical regions. Host association and biogeographical history have determined the high species richness ofRhabdochonain Mexico. One of these species,Rhabdochona mexicana, is highly specific to the characid genusAstyanax.Characids are a group of freshwater fish with Neotropical affinity. In this paper, we explore the genetic diversity ofR. mexicanathrough samples obtained from populations ofAstyanaxspp. across river basins of Mexico and Guatemala. Sequences of one mitochondrial and two ribosomal genes were obtained from 38 individuals and analysed using Maximum Likelihood and Bayesian Inference analysis. Phylogenetic analyses usingcox1, and a concatenated alignment of 18S + 28S +cox1 recovered two genetic lineages. One of them corresponded withR. mexicana sensu stricto; this lineage included three reciprocally monophyletic subgroups; the other lineage was highly divergent and represented a putative candidate species. A detailed morphological study was conducted to corroborate the molecular findings. We describe a new species herein and discuss the implications of using molecular tools to increase our knowledge about the diversity of a speciose genus such asRhabdochona.

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A new species of blind Trechinae from the Pyrenees of Huesca, and its position within Aphaenops (sensu stricto) (Coleoptera: Carabidae: Trechini)

Zootaxa ◽

10.11646/zootaxa.2566.1.5 ◽

2010 ◽

Vol 2566 (1) ◽

pp. 49 ◽

Cited By ~ 6

Author(s):

ARNAUD FAILLE ◽

CHARLES BOURDEAU ◽

JAVIER FRESNEDA

Keyword(s):

New Species ◽

Molecular Data ◽

Sensu Stricto ◽

Sister Taxon ◽

Mitochondrial Coi ◽

First Time ◽

A New Species

A new trechine species Aphaenops parvulus sp. n. (Carabidae, Trechini) is described from Esjamundo cave in the Pyrenees of Huesca, Spain. The new species belongs to the subgenus Aphaenops (sensu stricto), but differs from its closest congeners by the small size—it is the smallest species of the group—and characters of the aedeagus. Molecular data based on fragments of a mitochondrial (COI) and a nuclear (LSU) genes recognised Aphaenops parvulus sp. n. as a sister taxon to A. eskualduna Coiffait. Aphaenops eskualduna is reported from Spain with precision for the first time.

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Redescriptions of Pugettia quadridens (De Haan, 1837) and P. intermedia Sakai, 1938 (Crustacea: Brachyura: Epialtidae) with description of a new species

Zootaxa ◽

10.11646/zootaxa.4672.1.1 ◽

2019 ◽

Vol 4672 (1) ◽

pp. 1-68

Author(s):

NAOYA OHTSUCHI ◽

TOMOHIKO KAWAMURA

Keyword(s):

New Species ◽

Sexual Dimorphism ◽

Morphological Characters ◽

Sensu Stricto ◽

Considerable Variability ◽

Local Variant ◽

Distribution Ranges ◽

Northeast Asian ◽

Orbital Region ◽

A New Species

Pugettia quadridens (De Haan, 1837) and its closely similar congener, P. intermedia Sakai, 1938, are redescribed and illustrated and their possible infraspecific variations related to sexual, ontogenetic, and environmental differences are discussed. As a result, a new species, Pugettia ferox, which had long been believed to be a local variant of P. quadridens sensu lato in the northeast Japan, is described. Despite moderate sexual dimorphism and considerable variability related to their ontogeny, the three species can be distinguished from each other by the combination of various morphological characters including the structure of the orbital region and male first gonopods. The patterns of ontogenetic morphological and morphometric changes in the chelipeds, gonopods, and pleons are also differentiated among the three species. The updated distribution ranges of three species greatly overlap in the northeast Asian waters, although P. quadridens sensu stricto is distributed more southward than P. ferox n. sp.. Distinguishing characters between P. quadridens s.s. and P. pellucens Rathbun, 1932, and between P. intermedia and P. vulgaris Ohtsuchi, Kawamura & Takeda, 2014, are revised.

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A review of some ticks (Acarina: Ixodidae) of sea birds Part II. The taxonomic problems associated with the Ixodes auritulus-percavatus group of species

Parasitology ◽

10.1017/s0031182000025294 ◽

1960 ◽

Vol 50 (1-2) ◽

pp. 199-226 ◽

Cited By ~ 10

Author(s):

D. R. Arthur

Keyword(s):

New Species ◽

Sensu Stricto ◽

Marion Island ◽

Tristan Da Cunha ◽

Sea Birds ◽

A New Species

1. Ixodes auritulus group of ticks and Ixodes percavatus group of ticks are both characterized by having the palpal article I drawn out to a horn anteriorly, but in the percavatus group there is a strong meso-dorsal ‘spur’ on this article, which is lacking in the auritulus group.2. The mutilated types of I. auritulus and I. percavatus sp. revocata are re-described and electotypes established for both.3. Variations from I. auritulus sensu stricto are described from Peru and Guatemala, and a revised description given of the male, female and nymph from Snares Island.4. I. percavatus and I. zumpti sp.nov. have been diagnosed from material from Nightingale Island, Tristan da Cunha. Ticks from Marion Island are identified as a new species, I. pterodromae. Female and nymphs from the Paris Museum originally identified by Neumann as I. auritulus now fall into the percavatus group and are named I. cornuae sp.nov.

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Molecular Typing of Borrelia burgdorferiSensu Lato: Taxonomic, Epidemiological, and Clinical Implications

Clinical Microbiology Reviews ◽

10.1128/cmr.12.4.633 ◽

1999 ◽

Vol 12 (4) ◽

pp. 633-653 ◽

Cited By ~ 265

Author(s):

Guiqing Wang ◽

Alje P. van Dam ◽

Ira Schwartz ◽

Jacob Dankert

Keyword(s):

Restriction Analysis ◽

Clinical Manifestations ◽

Taxonomic Status ◽

Sensu Stricto ◽

Borrelia Burgdorferi Sensu Lato ◽

Clinical Implications ◽

Rrna Gene ◽

Enzyme Electrophoresis ◽

Borrelia Lusitaniae ◽

Dna Reassociation

SUMMARY Borrelia burgdorferi sensu lato, the spirochete that causes human Lyme borreliosis (LB), is a genetically and phenotypically divergent species. In the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the LB-related spirochetes and their potential association with distinct clinical syndromes. These methods include serotyping, multilocus enzyme electrophoresis, DNA-DNA reassociation analysis, rRNA gene restriction analysis (ribotyping), pulsed-field gel electrophoresis, plasmid fingerprinting, randomly amplified polymorphic DNA fingerprinting analysis, species-specific PCR and PCR-based restriction fragment length polymorphism (RFLP) analysis, and sequence analysis of 16S rRNA and other conserved genes. On the basis of DNA-DNA reassociation analysis, 10 different Borrelia species have been described within the B. burgdorferi sensu lato complex: B. burgdorferi sensu stricto, Borrelia garinii, Borrelia afzelii, Borrelia japonica, Borrelia andersonii, Borrelia valaisiana, Borrelia lusitaniae, Borrelia tanukii, Borrelia turdi, and Borrelia bissettii sp. nov. To date, only B. burgdorferi sensu stricto, B. garinii, and B. afzelii are well known to be responsible for causing human disease. Different Borrelia species have been associated with distinct clinical manifestations of LB. In addition, Borrelia species are differentially distributed worldwide and may be maintained through different transmission cycles in nature. In this paper, the molecular methods used for typing of B. burgdorferi sensu lato are reviewed. The current taxonomic status of B. burgdorferi sensu lato and its epidemiological and clinical implications, especiallly correlation between the variable clinical presentations and the infecting Borrelia species, are discussed in detail.

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