The process of discovery in and new species from northern Peru, Cerro Colán, Department Amazonas, Province Bagua

A review of discoveries of plants and animals by a 1978 expedition from Louisiana State University to Peru is presented. Genesis of this study was owing to the senior author’s observation of unusually high species richness in Araceae among a collection of plants made in northern Peru. A subsequent review showed that the region was rich in many ways. Determinations to date include 983 species belonging to 513 genera and 145 families of which 81 species are endemic. Included are 59 plant taxa new to science described elsewhere. Eleven aroids (Araceae) from northern Peru are described and illustrated as new. Some records represent new taxa described from voucher specimens independently collected by botanists at prior or later dates and different localities. A collection was assigned the nov. sp. category of noteworthiness if Tropicos database (http://www.tropicos.org) showed either the Alwyn H. Gentry, et al. and or the Philip J. Barbour accession to be the first such collection known. Specimen searches in Tropicos by senior collector as Philip Barbour and separately by Gentry and constrained by appropriate dates revealed 1687 independent determined voucher specimens of which 1545 are noteworthy (92%) by the designated categories. Noteworthy categories are described and presented in appendices 2 and 3. Extralimital plant distribution records are not described here. Four new bird species/subspecies and seven new frog species were discovered on this expedition. It is important to note that after only 42 years much of the region where many of these discoveries were made is now largely devoid of natural vegetation. Appendix 4 provides detailed descriptions of habitat on Cerro Colán as recent as 2017. This study is a reminder that areas newly opened for exploration should be thoroughly and quickly studied to capture the greatest scientific benefit. It shows how much could be attained in a small span of time by a small but dedicated group of biologists.

Hoverflies (Syrphidae: Diptera) in areas contaminated with heavy metals (Cd, Zn, Pb)

The aim of the research was to study the diversity of hoverflies (Syrphidae: Diptera) living in areas under the influence of heavy metal emissions (Cd, Zn and Pb). Although the area of the Olkusz Ore-bearing Region (OOR), where the research was partially conducted, belongs to the contaminated regions, where a relatively high species richness of Syrphids has been noted. During the research period, a total of 1,180 syrphids, belonging to 165 species were caught, which constitutes 39.76% of all the Polish Syrphidae fauna. These species represented all the trophic groups with the dominance of zoophagous species (48.48%); however, the most numerous (quantitatively abundant) were saprophagous. Among the collected syrphids, common and numerous species, such as: E. arbustorum (LINNAEUS, 1758), E. tenax (LINNAEUS, 1758), Episyrphus balteatus (DE GEER, 1776) and S. pipiens (LINNAEUS, 1758), as well as rare species such as Callicera aenea (FABRICIUS, 1777) (NT), Chalcosyrphus piger (FABRICIUS, 1794) (DD), Epistrophe ochrostoma (ZETTERSTEDT, 1849) (VU), Orthonevra geniculata (MEIGEN, 1830) (DD), Rhingia rostrata (LINNAEUS, 1758) (DD), Sphegina sibirica STACKELBERG, 1953 (LC) and Spilomyia diophthalma (LINNAEUS, 1758) (NT), were noted. Eleven of the collected species have been included in the Polish Red List of Endangered Species. Melangyna ericarum (COLLIN, 1946) is recorded as a new one among the Polish fauna. Numerous observations did not indicate that the area covered by the study was characterised by a significantly lower species diversity compared to other sites. In fact, the samples taken were usually characterised by a relatively high biodiversity. The obtained results may suggest that anthropogenic activities, including those leading to environmental pollution with heavy metals and to a strong transformation of natural habitats, do not completely destroy biodiversity, and in some cases leave space for nature to create habitats where even rare species of organisms such as Syrphidae can develop.

FAUNA AND BIOTOPIC DISTRIBUTION OF WEEVILS (COLEOPTERA: CURCULIONOIDEA) OF THE TALOVSKAYA STEPPE SITE OF THE ORENBURG STATE NATURE RESERVE

Based on research results 2018-2020 the species composition was established and the analysis of the fauna of weevils (Curculionoidea) of the Talovskaya steppe site of the Orenburg natural reserve was carried out. The research area is located in the very south-west of the Orenburg region near the border with Kazakhstan, in the subzone of the southern steppes of the East of the Russian Plain. 199 species of weevils from 4 families were identified (27 % of the fauna of the Orenburg region). Of these, 9 species are first reported for the fauna of the Orenburg Province. The characteristic features of the weevil fauna of the Talovskaya steppe are: a high proportion of Central Palaearctic species (35 %) (with an insignificant participation of Western Palaearctic forms); a sharp predominance of species of the subboreal complex (steppe and desert-steppe) (58 %) (with a very small number of forest species) and a noticeable proportion of species of the halophilic complex. The data presented in the article allow us to consider the fauna of the Talovskaya steppe as a typical southern steppe fauna. It is characterized by a fairly high species richness and the presence of a number of rare species. This is confirmed by the importance of the protected area for the preservation of the peculiar natural complexes of the southwestern outskirts of Obshchy Syrt.

Levantamento da fauna de abelhas (Hymenoptera, Apoidea) em um fragmento de Mata Atlântica, no município de Ubá, Minas Gerais

Realizou-se um levantamento da fauna de abelhas ocorrentes em um fragmento de Mata Atlântica no município de Ubá – MG. O estudo foi realizado em um período de 12 meses. Para a amostragem das espécies foram utilizadas armadilhas odoríferas em garrafas pet, armadilhas de nidificação e coletas em voo. Foram amostrados 40 indivíduos pertencentes a 13 espécies. Dentre essas, três consideradas sociais, uma primitivamente social e nove espécies de hábito solitário. Apesar de próximo à área urbana, a riqueza de espécie foi considerada alta. Novos estudos no município e em áreas adjacentes devem ser conduzidos com a finalidade de ampliar o conhecimento sobre as populações de abelhas da região e assim reunir dados que possam contribuir para a execução de estratégias de manejo visando à conservação desses polinizadores. We surveyed the bee fauna in an Atlantic forest fragment at Ubá municipality, Minas Gerais state, southeastern Brazil. We study was conducted over a 12-month period. For species sampling, we used scent traps made with PET bottles, trap-nests, and targeted sweep netting. We sampled 40 individuals from 13 species. Three species are social, one is primitively social and nine are solitary. Although the sampling site is located near the urban perimeter, it showed high species richness. Further studies in the municipality and in adjacent areas must be conducted in order to increase knowledge of the bee population in the region, thus enabling the compilation of a dataset that might contribute with management practices aimed at the conservation of these important pollinators.

DNA Metabarcoding Methods for the Study of Marine Benthic Meiofauna: A Review

Meiofaunal animals, roughly between 0.045 and 1 mm in size, are ubiquitous and ecologically important inhabitants of benthic marine ecosystems. Their high species richness and rapid response to environmental change make them promising targets for ecological and biomonitoring studies. However, diversity patterns of benthic marine meiofauna remain poorly known due to challenges in species identification using classical morphological methods. DNA metabarcoding is a powerful tool to overcome this limitation. Here, we review DNA metabarcoding approaches used in studies on marine meiobenthos with the aim of facilitating researchers to make informed decisions for the implementation of DNA metabarcoding in meiofaunal biodiversity monitoring. We found that the applied methods vary greatly between researchers and studies, and concluded that further explicit comparisons of protocols are needed to apply DNA metabarcoding as a standard tool for assessing benthic meiofaunal community composition. Key aspects that require additional consideration include: (1) comparability of sample pre-treatment methods; (2) integration of different primers and molecular markers for both the mitochondrial cytochrome c oxidase subunit I (COI) and the nuclear 18S rRNA genes to maximize taxon recovery; (3) precise and standardized description of sampling methods to allow for comparison and replication; and (4) evaluation and testing of bioinformatic pipelines to enhance comparability between studies. By enhancing comparability between the various approaches currently used for the different aspects of the analyses, DNA metabarcoding will improve the long-term integrative potential for surveying and biomonitoring marine benthic meiofauna.

AVIFAUNA DO SOLO SAGRADO DO GUARAPIRANGA (SÃO PAULO, BRASIL)

AVIFAUNA OF THE SACRED SOIL OF GUARAPIRANGA (SÃO PAULO, BRAZIL): The avifauna of São Paulo, the largest Brazilian city, is one of the most studied in Brazil. However, some areas are lacking basic studies on the avifauna, including those under intense pressure from urban growth and deforestation. Here we present a list of bird species occurring in the Solo Sagrado do Guarapiranga (23°45’00”S, 46°44’00”W), an urban park in southern São Paulo. We carried out monthly bird surveys from July 2008 to June 2009, using transect method. After 250 hours of observations, we recorded 180 species, including 31 species endemics to Atlantic Forest and two species that are globally threatened. The insectivorous, omnivorous and frugivorous species were the most represented feeding guilds, with 70, 39 and 21 species, respectively. Occasional and rare species composed the community majority. The high species richness and the presence of threatened species indicate the importance of the area to the bird’s conservation in the largest Brazilian city.

Diversity, species richness distribution and centers of endemism of Lycianthes (Capsiceae, Solanaceae) in Mexico

Spatial patterns of plant diversity matter. Identifying areas of high species richness and endemism are crucial for understanding the evolution of plant diversity and community assemblages as well establishing sustainablility and conservation priorities. Lycianthes is the third most species-rich genus in the Solanaceae with 187 taxa (152 species, 10 subspecies and 25 varieties) native to the Americas and Asia-Pacific region. However, the distributions of the taxa are uneven and usually concentrated along mountain ranges in the Neotropic. In Mexico, the main mountain chains are known as the Mexican Transition Zone (MTZ), so we expected the highest species richness and endemism to be found there. Our aims were twofold; first, to determine the Lycianthes species richness in Mexico; and second, to analyze the spatial patterns. We used 2305 georeferenced records obtained from specimens examined in herbaria, electronic specimen databases and specimens cited in the literature. Then, we analyzed species richness and endemism using a grid-cell of 50 × 50 km, political divisions, biogeographic provinces, vegetation types, and elevation. In addition, endemism was quantified using weighted endemism (WE) and corrected weighted endemism (CWE). In Mexico, there were 48 taxa of Lycianthes, which included 44 species and four varieties. Of these, 20 species and two varieties were endemic. We found 14 cells of high species richness and eight centers of endemism, all located across the MTZ. The pine-oak forest resulted in the richest plant community followed by the cloud forest along an elevational gradient of 1000–2000 m. Likewise, endemism was found within the pine-oak forest of the MTZ, between 1000–2000 m of elevation. The geological history and climatic conditions of the MTZ could explain the diversity of Lycianthes there.

High Species Richness and Extremely Low Abundance of Cumacean Communities Along the Shelf and Slope of the Gulf of Guinea (West Africa)

The Gulf of Guinea belongs to the most scarcely sampled marine basins in the oceans of the world. We have analyzed diversity and distribution patterns of cumacean communities on the shelf and slope, along the coast of Ghana. The material was collected in October and November of 2012 using a van Veen grab (0.1 m2) on nine transects. Six stations were located at each transect (25, 50, 100, 250, 500, and 1,000 m). Sixty-three species of Cumacea were recorded with Leucon and Eocuma as the most speciose genera, with 12 and eight species, respectively. Comparisons of species richness with literature data pointed that the Ghanaian coast hosts very diverse communities. About 95% of species were new to science, and the number of cumacean species known from the West Africa increased by over 100%. Nevertheless, most of the species had extremely low abundance, 13 singletons and 15 doubletons were found. Mean density of cumaceans was estimated at only 1.5 ind./0.1 m2. Species accumulation curve did not reach the asymptotic level, suggesting undersampling, despite the fact that sampling effort was high (250 samples). The highest species richness was recorded in the inner shelf (25–50 m) and on the slope (1,000 m). Cluster analysis separated shallow water communities from deeper regions on the shelf and upper slope. The most unique species composition was found at 1,000 m. Principal component analysis showed the importance of oxygen, sediments, and human-related disturbance for distribution of cumacean communities. In the shallows, oxygen content and presence of gravel were the most important factors structuring communities. In the deeper bottom areas (250–1,000 m), cumacean fauna was affected by local pollution, mainly by higher concentration of barium, other heavy metals, and THC.

Assessing the diversity of whiteflies infesting cassava in Brazil

Background The necessity of a competent vector for transmission is a primary ecological factor driving the host range expansion of plant arthropod-borne viruses, with vectors playing an essential role in disease emergence. Cassava begomoviruses severely constrain cassava production in Africa. Curiously, begomoviruses have never been reported in cassava in South America, the center of origin for this crop. It has been hypothesized that the absence of a competent vector in cassava is the reason why begomoviruses have not emerged in South America. Methods We performed a country-wide whitefly diversity study in cassava in Brazil. Adults and/or nymphs of whiteflies were collected from sixty-six cassava fields in the main agroecological zones of the country. A total of 1,385 individuals were genotyped based on mitochondrial cytochrome oxidase I sequences. Results A high species richness was observed, with five previously described species and two putative new ones. The prevalent species were Tetraleurodes acaciae and Bemisia tuberculata, representing over 75% of the analyzed individuals. Although we detected, for the first time, the presence of Bemisia tabaci Middle East-Asia Minor 1 (BtMEAM1) colonizing cassava in Brazil, it was not prevalent. The species composition varied across regions, with fields in the Northeast region showing a higher diversity. These results expand our knowledge of whitefly diversity in cassava and support the hypothesis that begomovirus epidemics have not occurred in cassava in Brazil due to the absence of competent vector populations. However, they indicate an ongoing adaptation process of BtMEAM1 to cassava, increasing the likelihood of begomovirus emergence in this crop.