scholarly journals Erigeron bonariensis: hospedeira alternativa do Lettuce mosaic virus no Brasil

2003 ◽  
Vol 28 (3) ◽  
pp. 307-311 ◽  
Author(s):  
Alexandre L. R. Chaves ◽  
Marina R. Braun ◽  
Marcelo Eiras ◽  
Addolorata Colariccio ◽  
Silvia R. Galleti
Keyword(s):  
Mosaic Virus ◽  
Lactuca Sativa ◽  
Nicotiana Benthamiana ◽  
Tomato Spotted Wilt Virus ◽  
Potato Virus Y ◽  
Turnip Mosaic Virus ◽  
Lettuce Mosaic Virus ◽  
Chenopodium Amaranticolor ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

O gênero Erigeron (Asteraceae), de plantas da vegetação espontânea, encontra-se amplamente disseminado nas regiões Sul e Sudeste do Brasil, sendo freqüentemente encontrado em lavouras perenes e anuais. Plantas de E. bonariensis com sintoma de mosaico, típico do induzido por vírus, foram coletadas no município de São Paulo e submetidas a análises ao microscópio eletrônico de transmissão, testes biológicos, sorológicos e moleculares. Em cortes ultrafinos do tecido foliar original, observaram-se inclusões tubulares e cata-ventos dispersos no citoplasma. Através de inoculação mecânica, somente Chenopodium amaranticolor, C. quinoa, Nicotiana benthamiana e N. clevelandii foram infetadas. Os resultados obtidos em ELISA foram negativos quando se utilizaram antissoros contra o Turnip mosaic vírus (TuMV) e diferentes estirpes do Potato virus Y (PVY), constatando-se relacionamento sorológico com o Lettuce mosaic virus (LMV). Com a utilização de oligonucleotídeos específicos para LMV amplificaram-se fragmentos esperados de aproximadamente 280 pb, que seqüênciados confirmaram a identidade do vírus. A ocorrência do LMV em E. bonariensis, gênero da mesma família botânica da alface (Lactuca sativa), é de grande importância, pois talvez possa atuar como reservatório para infecção de campos de produção de alface. Este é o primeiro relato, no Brasil, de vírus infetando Erigeron sp., o qual só havia sido reportado como hospedeira natural do Bidens mottle virus (BiMoV) e do Tomato spotted wilt virus (TSWV) nos Estados Unidos.

scholarly journals Incidence of viruses infecting pepper in Thailand

2019 ◽  
Vol 10 (1) ◽  
pp. 184-193
Author(s):  
Amisa Laprom ◽  
Somrudee Nilthong ◽  
Ekachai Chukeatirote
Keyword(s):  
Mosaic Virus ◽  
Mixed Infection ◽  
Tomato Spotted Wilt Virus ◽  
Potato Virus Y ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mixed Infections ◽  
The North ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Chilli Veinal Mottle Virus

AbstractThis study was conducted to determine the incidence, diversity and distribution of viruses infecting pepper (Capsicum spp.) in the central, northern and northeastern parts of Thailand. During a survey in 2016 - 2019, a total of 2,149 leaf samples from symptomatic and asymptomatic peppers were collected randomly from farmer’s fields, and preliminary tested by an enzyme-linked immunosorbent assay (ELISA) using 7 antibodies specific for cucumber mosaic virus (CMV), chilli veinal mottle virus (ChiVMV), tomato necrotic ringspot virus (TNRV), tobacco mosaic virus (TMV), potato virus Y (PVY), tomato spotted wilt virus (TSWV), and begomoviruses. Our data revealed that the incidence of the viruses infecting pepper in Thailand was high, accounting for nearly 70% (1,482 infected samples). The highest viral incidence was found in the central part (96%), followed by the north (74.4%) and the northeastern (52.8%), respectively. Begomoviruses, CMV, ChiVMV, and TNRV were detected in the samples at varying rates, whereas PVY, TMV, and TSWV were not detected. Of these, the most frequently found virus was Begomoviruses accounting for nearly 33%, with the highest rate (ca. 82%) in the central Provinces of Thailand. In addition, of the 1,482 infected samples, mixed infections among the four viruses were also found in 616 samples (ca. 42%), and CMV + ChiVMV (approximately 11%) was the most common mixed infection. This is the first report describing an occurrence of viruses in pepper of Thailand, and the results obtained have revealed that viruses infecting pepper are widespread, which may pose a threat to pepper production in Thailand.

scholarly journals Alfalfa Mosaic Virus RNAs Serve as Cap Donors for Tomato Spotted Wilt Virus Transcription during Coinfection ofNicotiana benthamiana

1999 ◽  
Vol 73 (6) ◽  
pp. 5172-5175 ◽  
Author(s):  
Daniël Duijsings ◽  
Richard Kormelink ◽  
Rob Goldbach
Keyword(s):  
Mosaic Virus ◽  
Nicotiana Benthamiana ◽  
Tomato Spotted Wilt Virus ◽  
Alfalfa Mosaic Virus ◽  
Sequence Specificity ◽  
Reverse Transcription Pcr ◽  
Virus Transcription ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

ABSTRACT Tomato spotted wilt virus (TSWV) was shown to use alfalfa mosaic virus (AMV) RNAs as cap donors in vivo during a mixed infection inNicotiana benthamiana. By use of nested reverse transcription-PCR, TSWV N and NSs mRNAs provided with capped leader sequences derived from all four AMV RNAs could be cloned and sequenced. The sequence specificity of the putative TSWV endonuclease involved is discussed.

scholarly journals Bakterijske i virusne bolesti rajčice i paprike u Hrvatskoj

2019 ◽  
Vol 42 (3) ◽  
pp. 36-42
Author(s):  
Dario Ivić
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Pseudomonas Syringae ◽  
Tomato Spotted Wilt Virus ◽  
Clavibacter Michiganensis ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

U proteklih nekoliko godina u Hrvatskoj su zabilježene relativno visoke štete od pojedinih bakterijskih i virusnih bolesti u proizvodnji rajčice i paprike. Uzročnici tih bolesti bile su bakterije Pseudomonas syringae pv. tomato i Clavibacter michiganensis ssp. michiganensis te virusi Tomato spotted wilt virus (TSWV) i Cucumber mosaic virus (CMV). U članku su ukratko opisani spomenuti patogeni, njihovo širenje te mjere zaštite.

BREEDING TRANSGENIC TOMATOES FOR RESISTANCE TO TOMATO SPOTTED WILT VIRUS AND CUCUMBER MOSAIC VIRUS

1996 ◽  
pp. 442-448 ◽  
Author(s):  
C. Gonsalves ◽  
B. Xue ◽  
S.Z. Pang ◽  
R. Provvidenti ◽  
J.L. Slightom ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Spotted Wilt ◽  
Transgenic Tomatoes ◽  
Wilt Virus

Small RNA profiles of wild-type and silencing suppressor-deficient tomato spotted wilt virus infected Nicotiana benthamiana

2015 ◽  
Vol 208 ◽  
pp. 30-38 ◽  
Author(s):  
Paolo Margaria ◽  
Laura Miozzi ◽  
Cristina Rosa ◽  
Michael J. Axtell ◽  
Hanu R. Pappu ◽  
...  
Keyword(s):  
Small Rna ◽  
Nicotiana Benthamiana ◽  
Tomato Spotted Wilt Virus ◽  
Silencing Suppressor ◽  
Wild Type ◽  
Tomato Spotted Wilt ◽  
Wilt Virus

scholarly journals Effects of Temperature and Host on the Generation of Tomato Spotted Wilt Virus Defective Interfering RNAs

1997 ◽  
Vol 87 (11) ◽  
pp. 1168-1173 ◽  
Author(s):  
Alice K. Inoue-Nagata ◽  
Richard Kormelink ◽  
Tatsuya Nagata ◽  
Elliot W. Kitajima ◽  
Rob Goldbach ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Nicotiana Benthamiana ◽  
Tomato Spotted Wilt Virus ◽  
Large Population ◽  
Datura Stramonium ◽  
Rna Molecules ◽  
Tomato Spotted Wilt ◽  
Effects Of Temperature ◽  
High Virus ◽  
Wilt Virus

The generation of defective interfering (DI) RNA molecules of tomato spotted wilt tospovirus (TSWV) was studied by serially passaging in-ocula from plant to plant under different controlled conditions. DI RNAs were generated at higher rates in plants at 16°C than in plants incubated at higher temperatures. Another factor promoting the TSWV DI RNA generation was the use of high virus concentrations in the inocula. The solanaceous species Capsicum annuum, Datura stramonium, Lycopersicon esculentum, Nicotiana benthamiana, and N. rustica supported the generation of DI RNAs, whereas the virus recovered from the inoculated composite species, Emilia sonchifolia, remained free of any DI RNA under all conditions tested. This study resulted in a strategy to maintain DI RNA-free TSWV isolates, as well as in an efficient way to produce a large population of different DI RNA species. A single DI RNA species usually became dominant in an isolate after a few rounds of serial inoculations. The possible mechanisms involved in TSWV DI RNA generation under different inoculation circumstances are discussed.

scholarly journals New Breeding Lines Resistant to Tomato Mosaic Virus and Tomato Spotted Wilt Virus within the ‘De la Pera’ Tomato Type: UMH 1353 and UMH 1354

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 456-458 ◽  
Author(s):  
Santiago García-Martínez ◽  
Adrián Grau ◽  
Aranzazu Alonso ◽  
Fernando Rubio ◽  
Pedro Carbonell ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Tomato Spotted Wilt Virus ◽  
Tomato Mosaic Virus ◽  
Breeding Lines ◽  
Tomato Spotted Wilt ◽  
Wilt Virus
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