scholarly journals Anatomia de lesões foliares causadas pelo vírus da Mancha Clorótica do Clerodendrum, transmitido pelo ácaro Brevipalpus phoenicis em diferentes espécies

2010 ◽  
Vol 36 (4) ◽  
pp. 291-297 ◽  
Author(s):  
Renata Takassugui Gomes ◽  
Elliot Watanabe Kitajima ◽  
Francisco André Osamu Tanaka ◽  
João Paulo Rodrigues Marques ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Ringspot Virus ◽  
Annona Muricata ◽  
Spot Virus ◽  
Orchid Fleck Virus ◽  
Chlorotic Spot ◽  
Brevipalpus Phoenicis ◽  
Citrus Leprosis

O gênero botânico Clerodendrum pertence à família Lamiaceae e compreende várias espécies ornamentais, Manchas cloróticas e necróticas em folhas de coração-sangrento foram observadas pela primeira vez em um jardim de Piracicaba, SP, associadas à infestação com Brevipalpus phoenicis (Acari: Tenuipalpidae). Exames de secções de tecidos das lesões foliares ao microscópio eletrônico revelaram ocorrência de efeitos citopáticos do tipo nuclear e concluiu-se que os sintomas eram causados por um vírus transmitido por Brevipalpus (VTB), o qual foi designado de mancha clorótica de Clerodendrum (Clerodendrum Chlorotic Spot Virus- ClCSV). O ClCSV é transmitido mecanicamente de coração-sangrento para coração-sangrento. Em ensaios preliminares foi transmitido por B. phoenicis e mecanicamente para várias outras plantas, além da ocorrência de sua disseminação natural por este ácaro para outras espécies. Visando complementar a caracterização do ClCSV foram feitos estudos sobre alterações anatômicas em folhas de plantas infectadas pelo ClCSV. Foram examinadas secções histológicas de folhas sadias e infectadas pelo ClCSV de C. x speciosum e de outras hospedeiras como Hibiscus schizopetalus, Salvia leucantha, Malvaviscus arboreus e Annona muricata. Constatou-se que o ClCSV causa alterações celulares semelhantes nas diferentes hospedeiras e os sintomas causados por este vírus são similares aos causados por outros vírus transmitidos por Brevipalpus como o vírus da leprose dos citros citoplasmático (Citrus Lepros Virus Cytoplasmic- CiLV-C) e nuclear (Citrus Leprosis Virus Nuclear- CiLV-N), mancha anular do cafeeiro (Coffee Ringspot Virus- CoRSV), mancha anular de Solanum violaefolium (Solanum violaefolium Ringspot Virus- SvRSV) e "Orchid Fleck Vírus" (OFV), representadas por hipertrofia e hiperplasia frequentemente acompanhadas de necrose nos tecidos do parênquima paliçádico e lacunoso.

scholarly journals Common Bean: Experimental Indicator Plant for Citrus leprosis virus C and Some Other Cytoplasmic-Type Brevipalpus-Transmitted Viruses

Plant Disease ◽  
2013 ◽  
Vol 97 (10) ◽  
pp. 1346-1351 ◽  
Author(s):  
L. C. Garita ◽  
A. D. Tassi ◽  
R. F. Calegario ◽  
E. W. Kitajima ◽  
S. A. M. Carbonell ◽  
...  
Keyword(s):  
Common Bean ◽  
Standard Test ◽  
Ringspot Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Green Spot ◽  
Citrus Leprosis ◽  
Cytoplasmic Type ◽  
Virus C

Citrus leprosis (CL) caused by Citrus leprosis virus C (CiLV-C) is present in Latin America from Mexico to Argentina, where citrus plants are grown. CiLV-C is transmitted by the tenuipalpid mite, Brevipalpus phoenicis, causing localized lesions on citrus leaves, fruit, and stems. One limitation to study of the virus–vector–host relationship in this pathosystem is the lack of a suitable assay plant. On Citrus spp. used as susceptible hosts, symptoms may take weeks or months to appear after experimental inoculation by viruliferous mites. Common bean (Phaseolus vulgaris) was found to respond with localized necrotic lesions after inoculation with viruliferous B. phoenicis in 5 days. Thus far, 113 tested common bean varieties and lines and some recent accessions of varied genetic background behaved in a similar way. Black bean ‘IAC Una’ was adopted as a standard test variety. When inoculated leaves were left at 28 to 30°C, the period for the lesion appearance was reduced to only 2 days. Confirmation that the lesions on common bean leaves are caused by CiLV-C were made by transmission electron microscopy, immunofluorescence, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction specific for CiLV-C. Common bean plants mite-inoculated with some other cytoplasmic-type Brevipalpus-transmitted viruses (BrTVs) (Passion fruit green spot virus, Solanum violaefolium ringspot virus, Ligustrum ringspot virus, and Hibiscus green spot virus) also responded with necrotic local lesions and may serve as test plants for these viruses. Two nuclear types of BrTV (Coffee ringspot virus and Clerodendrum chlorotic spot virus) were unable to produce symptoms on common bean.

scholarly journals Caracterização do Tomato chlorotic spot virus isolado de jiló no Vale do Paraíba, Estado de São Paulo

2002 ◽  
Vol 27 (3) ◽  
pp. 285-291 ◽  
Author(s):  
MARCELO EIRAS ◽  
ALEXANDRE L. R. CHAVES ◽  
ADDOLORATA COLARICCIO ◽  
RICARDO HARAKAVA ◽  
JANSEN DE ARAUJO ◽  
...  
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Sao Paulo ◽  
Ringspot Virus ◽  
São Paulo ◽  
Rt Pcr ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Das Elisa

Os tospovírus são responsáveis por perdas significativas em diversas culturas, principalmente solanáceas. No município de São José dos Campos (SP), plantas de jiló (Solanum gilo) apresentando sintomas de mosaico, bolhosidades, nanismo e queda acentuada da produção foram coletadas para análise. Visando a caracterização do agente causador dos sintomas, testes biológicos, elétrono microscópicos, sorológicos e moleculares foram realizados. Através de inoculação mecânica em plantas indicadoras das famílias Amaranthaceae, Chenopodiaceae e Solanaceae obtiveram-se resultados típicos aos esperados para tospovírus. Ao microscópio eletrônico de transmissão, observaram-se, em contrastação negativa, partículas pleomórficas com diâmetro entre 80 e 110 nm e em cortes ultra-finos partículas presentes em vesículas do retículo endoplasmático. Através de DAS-ELISA, identificou-se o Tomato chlorotic spot virus (TCSV). A partir de RNA total extraído de folhas infetadas, amplificaram-se, via RT-PCR, fragmentos correspondentes ao gene da proteína do capsídeo (cp) os quais foram seqüenciados e comparados com outros depositados no "GenBank". A homologia de nucleotídeos e aminoácidos deduzidos foi respectivamente de 99 e 95% quando comparada com seqüências de isolados de TCSV. A comparação com as outras espécies do gênero Tospovirus apresentou valores de homologia entre 72 e 84%. Estes resultados confirmam a identidade deste vírus como pertencente à espécie TCSV, que é predominante no Estado de São Paulo e importante patógeno de outras plantas cultivadas. Além disso, variedades de jiló quando inoculadas foram susceptíveis tanto ao TCSV como às espécies Tomato spotted wilt virus (TSWV) e Groundnut ringspot virus (GRSV).

scholarly journals Identification and Characterization of Citrus Chlorotic Spot Virus, a New Dichorhavirus Associated with Citrus Leprosis-Like Symptoms

Plant Disease ◽  
2018 ◽  
Vol 102 (8) ◽  
pp. 1588-1598 ◽  
Author(s):  
C. Chabi-Jesus ◽  
P. L. Ramos-González ◽  
A. D. Tassi ◽  
O. Guerra-Peraza ◽  
E. W. Kitajima ◽  
...  
Keyword(s):  
Virus Strain ◽  
Viral Vectors ◽  
Morphological Characterization ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Bipartite Genome ◽  
Citrus Leprosis ◽  
Average Size ◽  
Parenchymal Cells

Local chlorotic spots resembling early lesions characteristic of citrus leprosis (CL) were observed in leaves of two sweet orange (Citrus sinensis L.) trees in Teresina, State of Piauí, Brazil, in early 2017. However, despite the similarities, these spots were generally larger than those of a typical CL and showed rare or no necrosis symptoms. In symptomatic tissues, transmission electron microscopy revealed the presence of viroplasms in the nuclei of the infected parenchymal cells and rod-shaped particles with an average size of approximately 40 × 100 nm, resembling those typically observed during infection by dichorhaviruses. A bipartite genome of the putative novel virus, tentatively named citrus chlorotic spot virus (CiCSV) (RNA1 = 6,518 nucleotides [nt] and RNA2 = 5,987 nt), revealed the highest nucleotide sequence identity values with the dichorhaviruses coffee ringspot virus strain Lavras (73.8%), citrus leprosis virus N strain Ibi1 (58.6%), and orchid fleck virus strain So (56.9%). In addition to citrus, CiCSV was also found in local chlorotic lesions on leaves of the ornamental plant beach hibiscus (Talipariti tiliaceum (L.) Fryxell). Morphological characterization of mites recovered from the infected plants revealed at least two different types of Brevipalpus. One of them corresponds to Brevipalpus yothersi. The other is slightly different from B. yothersi mites but comprises traits that possibly place it as another species. A mix of the two mite types collected on beach hibiscus successfully transmitted CiCSV to arabidopsis plants but additional work is required to verify whether both types of flat mite may act as viral vectors. The current study reveals a newly described dichorhavirus associated with a citrus disease in the northeastern region of Brazil.

Analysis of the coding-complete genomic sequence of groundnut ringspot virus suggests a common ancestor with tomato chlorotic spot virus

2016 ◽  
Vol 161 (8) ◽  
pp. 2311-2316 ◽  
Author(s):  
Soledad de Breuil ◽  
Joaquín Cañizares ◽  
José Miguel Blanca ◽  
Nicolás Bejerman ◽  
Verónica Trucco ◽  
...  
Keyword(s):  
Common Ancestor ◽  
Genomic Sequence ◽  
Ringspot Virus ◽  
Complete Genomic Sequence ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Complete Genomic

scholarly journals Hipersensibilidade e necrose sistêmica em Nicotiana benthamiana transformada com o gene de resistência Sw-5 de tomateiro

2006 ◽  
Vol 31 (3) ◽  
pp. 247-253 ◽  
Author(s):  
Douglas Lau ◽  
Julio Cezar F. de Oliveira ◽  
Elene Y. Lau ◽  
Sérgio H. Brommonschenkel
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Nicotiana Benthamiana ◽  
Ringspot Virus ◽  
Necrosis Virus ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Chrysanthemum Stem Necrosis Virus ◽  
Stem Necrosis

O gene Sw-5 do tomateiro confere resistência a várias espécies de tospovírus e codifica uma proteína contendo domínios de ligação a nucleotídeos e repetições ricas em leucina. Tomateiros com Sw-5 exibem reações necróticas nas folhas inoculadas com tospovírus. Estas reações e a estrutura da proteína Sw-5 indicam que a resistência ocorre por meio do reconhecimento do patógeno e desencadeamento da resposta de hipersensibilidade. A capacidade de Sw-5 de conferir resistência a tospovírus em tabaco selvagem (Nicotiana benthamiana Domin.) foi avaliada em plantas transgênicas. Uma construção com a seqüência aberta de leitura de Sw-5 e sua região 3’ não-traduzida sob controle do promotor 35S do CaMV foi utilizada para transformação de N. benthamiana via Agrobacterium tumefaciens. Plantas de progênies R1 foram inoculadas com um isolado de tospovírus e avaliadas quanto à ocorrência de reação de hipersensibilidade e resistência à infecção sistêmica. Em uma progênie com segregação 3:1 (resistente:suscetível), foi selecionada uma planta homozigota e sua progênie avaliada quanto ao espectro da resistência a tospovírus. Plantas com o transgene exibiram resposta de hipersensibilidade 48 h após a inoculação, sendo resistentes à infecção sistêmica. O fenótipo da resistência foi dependente do isolado viral e um isolado de Tomato chlorotic spot virus (TCSV) causou necrose sistêmica em todas as plantas inoculadas, enquanto que isolados de Groundnut ringspot virus (GRSV) e um isolado relacionado a Chrysanthemum stem necrosis virus (CSNV) ficaram restritos ao sítio de infecção. Comparações do espectro da resistência obtido neste trabalho com aquele observado em outros membros da família Solanaceae indicam que as vias de transdução de sinais e as respostas de defesa ativadas por Sw-5 são conservadas dentro desta família e polimorfismos genéticos nas vias de transdução de sinais ou em componentes das respostas de defesa podem resultar em diferentes níveis de resistência.

scholarly journals Emergence of Groundnut ringspot virus and Tomato chlorotic spot virus in Vegetables in Florida and the Southeastern United States

2015 ◽  
Vol 105 (3) ◽  
pp. 388-398 ◽  
Author(s):  
Craig G. Webster ◽  
Galen Frantz ◽  
Stuart R. Reitz ◽  
Joseph E. Funderburk ◽  
H. Charles Mellinger ◽  
...  
Keyword(s):  
United States ◽  
New York ◽  
South Carolina ◽  
Southeastern United States ◽  
The United States ◽  
South Florida ◽  
Ringspot Virus ◽  
Spot Virus ◽  
Genomic Rnas ◽  
Chlorotic Spot

Groundnut ringspot virus (GRSV) and Tomato chlorotic spot virus (TCSV) are two emerging tospoviruses in Florida. In a survey of the southeastern United States, GRSV and TCSV were frequently detected in solanaceous crops and weeds with tospovirus-like symptoms in south Florida, and occurred sympatrically with Tomato spotted wilt virus (TSWV) in tomato and pepper in south Florida. TSWV was the only tospovirus detected in other survey locations, with the exceptions of GRSV from tomato (Solanum lycopersicum) in South Carolina and New York, both of which are first reports. Impatiens (Impatiens walleriana) and lettuce (Lactuca sativa) were the only non-solanaceous GRSV and/or TCSV hosts identified in experimental host range studies. Little genetic diversity was observed in GRSV and TCSV sequences, likely due to the recent introductions of both viruses. All GRSV isolates characterized were reassortants with the TCSV M RNA. In laboratory transmission studies, Frankliniella schultzei was a more efficient vector of GRSV than F. occidentalis. TCSV was acquired more efficiently than GRSV by F. occidentalis but upon acquisition, transmission frequencies were similar. Further spread of GRSV and TCSV in the United States is possible and detection of mixed infections highlights the opportunity for additional reassortment of tospovirus genomic RNAs.

scholarly journals Tomato Chlorotic Spot Virus (TCSV) Putatively Incorporated a Genomic Segment of Groundnut Ringspot Virus (GRSV) Upon a Reassortment Event

Viruses ◽  
2019 ◽  
Vol 11 (2) ◽  
pp. 187 ◽  
Author(s):  
João Silva ◽  
Athos de Oliveira ◽  
Mariana de Almeida ◽  
Richard Kormelink ◽  
Tatsuya Nagata ◽  
...  
Keyword(s):  
Evolutionary History ◽  
Nucleotide Diversity ◽  
Ringspot Virus ◽  
Parental Genotype ◽  
Biological Traits ◽  
Reassortment Event ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
History Of ◽  
Event Based

Tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV) share several genetic and biological traits. Both of them belong to the genus Tospovirus (family Peribunyaviridae), which is composed by viruses with tripartite RNA genome that infect plants and are transmitted by thrips (order Thysanoptera). Previous studies have suggested several reassortment events between these two viruses, and some speculated that they may share one of their genomic segments. To better understand the intimate evolutionary history of these two viruses, we sequenced the genomes of the first TCSV and GRSV isolates ever reported. Our analyses show that TCSV and GRSV isolates indeed share one of their genomic segments, suggesting that one of those viruses may have emerged upon a reassortment event. Based on a series of phylogenetic and nucleotide diversity analyses, we conclude that the parental genotype of the M segment of TCSV was either eliminated due to a reassortment with GRSV or it still remains to be identified.

Sequence Analysis of the Glycoproteins of Tomato Chlorotic Spot Virus and Groundnut Ringspot virus and Comparison with other Tospoviruses

Virus Genes ◽  
2004 ◽  
Vol 29 (3) ◽  
pp. 321-328 ◽  
Author(s):  
Fernanda lovato ◽  
Tatsuya nagata ◽  
Renato Resende ◽  
Ant�nio �vila ◽  
Alice Inoue-Nagata
Keyword(s):  
Sequence Analysis ◽  
Ringspot Virus ◽  
Spot Virus ◽  
Chlorotic Spot

scholarly journals HISTOPATHOLOGICAL PATTERNS OF LESIONS CAUSED BY TWO DICHORHAVIRUS

2020 ◽  
Vol 95 (3) ◽  
pp. 211-223
Author(s):  
João Paulo Rodrigues Marques ◽  
Aline Daniele Tassi ◽  
Gustavo Mortean Filippi ◽  
Renato Salaroli ◽  
Elliot Watanabe Kitajima
Keyword(s):  
Central Region ◽  
Infected Plant ◽  
Plant Viruses ◽  
Ringspot Virus ◽  
Histopathological Changes ◽  
Spot Virus ◽  
Chlorotic Spot ◽  
Tissue Organization ◽  
Leaf Tissues ◽  
Tissue Alterations

 Different from most plant viruses, Brevipalpus mite-transmitted viruses (BTV) cause localized infection, not being able to systemically invade the infected plant. To broaden the specter of the histopathological changes in leaf tissues induced by BTV infection, we examined the tissue organization of leaf lesions caused by the infection of two additional representatives of dichorhaviruses, Clerodendrum chlorotic spot virus (ClCSV) and Coffee ringspot virus (CoRSV). In general, tissue alterations within the lesions followed the pattern noticed in CiLV-C and CiLV-N infection.  In all analyzed lesions, necrotic cells form the central region that is surrounded by a chlorotic halo where hypertrophied cells commonly occur. Presence of reactive oxygen components were also a characteristic of the chlorotic halo. Though more cases of localized lesions on leaves infected by more BTV, remains to be analyzed, it seems that these lesions respond similarly upon infection by BTV, either Cile- or Dichorhavirus. 

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