scholarly journals Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

2013 ◽  
Vol 33 (7) ◽  
pp. 924-930 ◽  
Author(s):  
Patrícia C. Sousa ◽  
Erika A.A. Santos ◽  
Ana L.P. Souza ◽  
Gabriela L. Lima ◽  
Felipe F.P.C. Barros ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Sperm Morphology ◽  
Blue Staining ◽  
Image Analyzer ◽  
Pecari Tajacu ◽  
Staining Methods ◽  
Collared Peccary ◽  
Bengal Rose ◽  
Phenol Blue ◽  
Morphometric Evaluation

The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.

A stranger in the family? On the social behavior of a leucistic collared peccary (Pecari tajacu) with pigmented conspecifics

2019 ◽  
Vol 60 (2) ◽  
pp. 303-305
Author(s):  
Valdir Leite da Silva ◽  
José Cândido ◽  
José Nelson Campanha ◽  
Doraci R. de Oliveira ◽  
Carla Gheler-Costa ◽  
...  
Keyword(s):  
Social Behavior ◽  
The Social ◽  
The Family ◽  
Pecari Tajacu ◽  
Collared Peccary

scholarly journals Correlation between the DNA fragmentation index (DFI) and sperm morphology of infertile patients

2021 ◽  
Author(s):  
Alberto Ferrigno ◽  
Giovanni Ruvolo ◽  
Giuseppina Capra ◽  
Nicola Serra ◽  
Liana Bosco
Keyword(s):  
Dna Fragmentation ◽  
Sperm Morphology ◽  
Seminal Fluid ◽  
Image Analyzer ◽  
Fragmented Dna ◽  
Fragmentation Index ◽  
Group A ◽  
Group B ◽  
Infertile Patients ◽  
Dna Fragmentation Index

Abstract Purpose To evaluate the correlation between the DNA Fragmentation Index (DFI) and sperm morphology in patients undergoing ICSI, as a predictive parameter in reproductive outcomes. Methods A retrospective study was conducted on 125 infertile patients enrolled in a fertility clinic. Seminal characteristics were measured following the WHO guidelines (2010) for the examination of the seminal fluid. After collecting motile sperm population by pellet swim up, DFI was calculated and simultaneously associated with sperm morphology using in situ TUNEL assay and an image analyzer software in at least 250 spermatozoa for each patient. Results All subjects were divided into two groups according to a cutoff established, by choice, of the sperm DFI (15%): group A (< 15%) consisting of 65 patients and group B (≥ 15%) of 60 patients. Data were analyzed using non-parametric statistical methods. The results demonstrate that there is no statistical difference between the two groups in seminal characteristics. The collective data show a high significant correlation, suggesting that spermatozoa with abnormal morphology are the best candidates to contain DNA damage (p < 0.001). Also, when group A is compared with group B, an increased percentage of morphologically normal spermatozoa with fragmented DNA was observed in patients, with DFI values ≥ 15% (p < 0.001). Conclusion These results are aimed at providing an exact value of DFI in morphologically normal spermatozoa, which will be helpful to the embryologist in evaluating the risk of transferring, during the ICSI procedure, a spermatozoon whit normal morphology but fragmented DNA.

Progesterone and estradiol profiles in different reproductive stages of captive collared peccary ( Pecari tajacu ) females assessed by fecal metabolites

2017 ◽  
Vol 180 ◽  
pp. 121-126 ◽  
Author(s):  
Concepción Ahuja-Aguirre ◽  
Lorena López-deBuen ◽  
Susana Rojas-Maya ◽  
Bertha C. Hernández-Cruz
Keyword(s):  
Pecari Tajacu ◽  
Collared Peccary

scholarly journals Action of proteases of the nematophagous fungi Pochonia chlamydosporia on Ascaris suum eggs of collared peccary (Pecari tajacu)

2015 ◽  
Vol 9 (31) ◽  
pp. 1883-1886 ◽  
Author(s):  
Elias de Freitas Soares Filippe ◽  
Humberto de Queiroz Jose ◽  
Victor de Araujo Jackson ◽  
Gorete Ramos Rodrigues Maria ◽  
de Oliveira Tavela Alexandre ◽  
...  
Keyword(s):  
Ascaris Suum ◽  
Nematophagous Fungi ◽  
Pochonia Chlamydosporia ◽  
Pecari Tajacu ◽  
Collared Peccary

scholarly journals Analysis of the presence and location of mast cells in periapical cysts and periapical granulomas

2016 ◽  
Vol 64 (4) ◽  
pp. 376-381 ◽  
Author(s):  
Emerson Filipe de Carvalho NOGUEIRA ◽  
Elder Gyress Feitosa FARIAS ◽  
Luciano Barreto SILVA ◽  
Alexandrino Pereira dos SANTOS NETO ◽  
Emanuel Sávio de Souza ANDRADE ◽  
...  
Keyword(s):  
Mast Cells ◽  
Granulation Tissue ◽  
Blue Staining ◽  
Double Blind Study ◽  
Double Blind ◽  
Cross Sectional ◽  
Periapical Granuloma ◽  
Staining Methods ◽  
Periapical Cyst ◽  
Quantitative Descriptive

ABSTRACT Objective: The aim of the present study was to locate mast cells in chronic periapical lesions (granulomas and cysts) by using histochemical techniques and toluidine blue staining. Methods: A quantitative, descriptive, cross-sectional and retrospective research was performed. The sample was obtained from histopathological reports in the archives of the laboratory of surgical pathology of the University of Pernambuco between November 2014 and May 2015. Results: Sixteen cases of granuloma and 21 cases of periapical cysts were selected. The stained slides were analyzed by two examiners at different times, in a double-blind study. Mast cells were found in 13 (61.9%) of the periapical cyst cases, located in the capsule of the lesion. In the periapical granuloma cases, mast cells were found in eight cases (50%), located in the granulation tissue. Conclusion: Mast cells were detected in both cysts and periapical granuloma, located in the capsule and granulation tissue, respectively. Mast cells were more prevalent in periapical cysts than in periapical granuloma.

Collared peccary (Pecari tajacu ) are susceptible to porcine reproductive and respiratory syndrome virus (PRRSV)

2018 ◽  
Vol 65 (6) ◽  
pp. 1712-1719 ◽  
Author(s):  
Ramón Molina-Barrios ◽  
José Luevano-Adame ◽  
Yuly Alexandra Henao-Díaz ◽  
Luis Giménez-Lirola ◽  
Pablo Piñeyro ◽  
...  
Keyword(s):  
Respiratory Syndrome Virus ◽  
Pecari Tajacu ◽  
Collared Peccary

scholarly journals Anti-influenza A virus antibodies in Tayassuidae from commercial rearing farms in Brazil

2019 ◽  
Vol 49 (4) ◽  
Author(s):  
Thaís Gasparini Baraldi ◽  
Henrique Meiroz de Souza Almeida ◽  
Amanda Bonalume Cordeiro de Morais ◽  
Gabriel Yuri Storino ◽  
Hélio José Montassier ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Sus Scrofa ◽  
Influenza A ◽  
Indirect Elisa ◽  
Blood Samples ◽  
Tayassu Pecari ◽  
Pecari Tajacu ◽  
Serological Surveillance ◽  
Collared Peccary ◽  
Domestic Swine

ABSTRACT: Family Tayassuidae in the suborder Suina include two species of peccaries in Brazil: the white-lipped peccary (Tayassu pecari) and the collared peccary (Pecari tajacu). These animals share common pathogens with domestic swine (Sus scrofa); however, their role as potential carrier remains unclear. This study focused on detecting the prevalence of influenza A antibodies in Tayassu pecari and Pecari tajacu from commercial rearing farms from two states in Brazil. A set of 50 blood samples from Pecari tajacu and 55 from Tayassu pecari were analyzed using a commercial indirect ELISA in order to investigate anti influenza A antibodies. Pecari tajacu samples presented 22% (11/50) of seropositivity for the virus. Serological surveillance is an important tool to identify the presence and the spread of the influenza virus in feral pigs.

Ethogram and time-activity budget of the collared peccary (Pecari tajacu, Tayassuidae): implications for husbandry and welfare

2020 ◽  
Vol 54 (25-26) ◽  
pp. 1617-1635
Author(s):  
Mariane Mendes da Silva ◽  
Carlos Magno de Faria ◽  
Fernanda de Souza Sá ◽  
Dhiordan Deon Lovestain Costa ◽  
Beatriz Cristiana da Silva ◽  
...  
Keyword(s):  
Activity Budget ◽  
Time Activity ◽  
Pecari Tajacu ◽  
Collared Peccary

Staining methods for sections of epon-embedded tissues for light microscopy

1970 ◽  
Vol 48 (1) ◽  
pp. 189-191 ◽  
Author(s):  
C. Roland Leeson ◽  
Thomas S. Leeson
Keyword(s):  
Light Microscopy ◽  
Synthetic Resin ◽  
Electron Microscopic ◽  
Glass Slides ◽  
Staining Methods

Sections 0.5–2 μ thick are mounted on clean glass slides and allowed to dry. A number of staining procedures are described. After the sections are stained, permanent preparations are made by mounting them in a synthetic resin. The methods result in sections which are suitable for routine light microscopy and for comparison with adjacent electron microscopic sections.

scholarly journals Occupancy estimation of jaguar Panthera onca to assess the value of east-central Mexico as a jaguar corridor

Oryx ◽  
2013 ◽  
Vol 48 (1) ◽  
pp. 133-140 ◽  
Author(s):  
Lisanne S. Petracca ◽  
O. Eric Ramírez-Bravo ◽  
Lorna Hernández-Santín
Keyword(s):  
Large Scale ◽  
Prey Species ◽  
Landscape Connectivity ◽  
Site Occupancy ◽  
Sampling Unit ◽  
Eastern Coast ◽  
Panthera Onca ◽  
Occupancy Modelling ◽  
Pecari Tajacu ◽  
Collared Peccary

AbstractThe use of wildlife corridors to maintain landscape connectivity has become increasingly relevant to the conservation of wide-ranging species, including the jaguar Panthera onca. Jaguars are particularly threatened in Mexico, where corridor linkages are tenuous as a result of habitat fragmentation. Our study assessed a section of potential corridor south of the Sierra Madre Oriental in eastern Mexico. We conducted 245 interviews with local inhabitants in 140 36-km2 sampling units over a 5-month period and compiled detection histories for jaguars and five prey species: collared peccary Pecari tajacu, red brocket deer Mazama americana, white-tailed deer Odocoileus virginianus, spotted paca Agouti paca, and nine-banded armadillo Dasypus novemcinctus. These detection histories were then analysed using site occupancy modelling. Each sampling unit was assigned a probability of habitat use based on (1) the two smaller prey species (paca and armadillo) and (2) at least two of the larger prey species (collared peccary and two deer species) using habitat in that unit. This probability estimate was considered a proxy for the prey base of each sampling unit and therefore the unit's suitability as a jaguar corridor. Although the prey base in some areas appears adequate to support a jaguar population, large-scale development projects and the paucity of jaguar sign are major obstacles to this region's potential as a jaguar corridor. Our results suggest that the eastern coast of Mexico may not be a priority area for range-wide jaguar conservation.

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