scholarly journals Leucogram and serum acute phase protein concentrations in queens submitted to conventional or videolaparoscopic ovariectomy

2010 ◽  
Vol 62 (1) ◽  
pp. 86-91 ◽  
Author(s):  
A.E. Alves ◽  
A.P.C. Ribeiro ◽  
P.A. Di Filippo ◽  
M.F. Apparicio ◽  
J.J. Fagliari ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Acute Phase ◽  
Surgical Procedures ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
White Blood Cells ◽  
Conventional Technique ◽  
Blood Cell Count ◽  
Serum Samples ◽  
Phase Protein

Thirty health queens were submitted to ovariectomy by conventional technique or by videolaparoscopy. In order to study the intensity of inflammatory response by means of acute phase protein analysis and white blood cell count, serum samples were taken before and until 144 hours after the surgical procedures. The protein concentrations that were significantly increased 24 hours after surgical procedures were: ceruloplasmin, hemopexin, haptoglobin, and α1-acid glycoprotein, 69.8%, 103.5%, 117.3%, and 199.0%, respectively, for conventional ovariectomy; and 22.3%, 46.1%, 79.8%, and 74.6%, respectively, for laparoscopic ovariectomy. Therefore, inflammatory response was more intense in queens submitted to conventional ovariectomy. Results indicate that the increase or decrease in acute phase proteins, as well as in white blood cells count, may be useful in the evaluation of inflammatory response induced by these surgical procedures.

FETUIN, A NEGATIVE ACUTE PHASE PROTEIN, ATTENUATES TNF SYNTHESIS AND THE INNATE INFLAMMATORY RESPONSE TO CARRAGEENAN

Shock ◽  
2001 ◽  
Vol 15 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Michael Ombrellino ◽  
Haichao Wang ◽  
Huan Yang ◽  
Minghuang Zhang ◽  
Jaideep Vishnubhakat ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Acute Phase ◽  
Acute Phase Protein ◽  
Fetuin A ◽  
Phase Protein

scholarly journals The acute-phase response in (NZB X NZW)F1 and MRL/l MICE.

1982 ◽  
Vol 156 (4) ◽  
pp. 1268-1273 ◽  
Author(s):  
C Rordorf ◽  
H P Schnebli ◽  
M L Baltz ◽  
G A Tennent ◽  
M B Pepys
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Phase Response ◽  
Serum Amyloid ◽  
Human Rheumatoid Arthritis ◽  
Amyloid A ◽  
Serum Amyloid A Protein ◽  
Phase Protein

The acute-phase plasma protein response to disease activity in murine models of autoimmune lupus-like disease was investigated by measurement of the concentration of serum amyloid P component (SAP) in NZB X W and MRL/l mice. The levels of SAP, which is a major acute-phase protein in mice, did not rise at all in response to progression of disease in NZB X W mice between the ages of 1 and 9 mo. This resembles the behavior of acute-phase proteins such as C-reactive protein and serum amyloid A protein in human systemic lupus erythematosus, and just as in human lupus, where the occurrence of intercurrent microbial infection can stimulate an acute-phase response, so injection of bacterial lipopolysaccharide or casein into the NZB X W mice stimulated "normal" acute-phase SAP production. In marked contrast, MRL/l mice developed greatly increased levels of SAP, which correlated closely with progression of their pathology as they aged. The disease profile of the MRL/l strain includes rheumatoid factors and spontaneous polyarthritis and their SAP response resembles the behavior of acute phase proteins in human rheumatoid arthritis. Different patterns of acute-phase response in different autoimmune disorders may thus be a reflection of the genetic predisposition to particular diseases and/or contribute to their pathogenesis. The existence of animal counterparts for the various clinical patterns of human acute-phase protein production will assist in experimental investigation of the underlying mechanisms and of the biological role of the acute-phase response.

scholarly journals Acute phase protein quantitation in serum samples from healthy Atlantic bottlenose dolphins (Tursiops truncatus)

2012 ◽  
Vol 25 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Carolyn Cray ◽  
Kristopher L. Arheart ◽  
Michael Hunt ◽  
Tonya Clauss ◽  
Lynda L. Leppert ◽  
...  
Keyword(s):  
Acute Phase ◽  
Tursiops Truncatus ◽  
Acute Phase Protein ◽  
Bottlenose Dolphins ◽  
Serum Samples ◽  
Protein Quantitation ◽  
Phase Protein

scholarly journals Interleukin-6 Downregulates Factor XII Production by Human Hepatoma Cell Line (HepG2)

Blood ◽  
1997 ◽  
Vol 90 (4) ◽  
pp. 1501-1507
Author(s):  
Franca Citarella ◽  
Angelina Felici ◽  
Mieke Brouwer ◽  
John Wagstaff ◽  
Antonio Fantoni ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Factor Xii ◽  
Human Hepatoma Cell ◽  
Phase Protein ◽  
Hepatoma Cell Line Hepg2

Involvement of the contact system of coagulation in the pathogenesis of various inflammatory diseases is suggested by reduced plasma levels of factor XII (Hageman factor) and prekallikrein generally considered to result from activation of the contact system. However, in many of these diseases patients develop an acute-phase response and, therefore, an alternative explanation for the decreased levels of factor XII could be the downregulation of factor XII gene expression in the liver as described for negative acute-phase proteins. We report here that interleukin-6 (IL-6), the principal cytokine mediating the synthesis of most acute-phase proteins in the liver, downregulates the production of factor XII by the human hepatoma cell line HepG2 by up to 75%. The decrease in protein secretion correlated with an equivalent decrease of factor XII mRNA likely indicating a pretranslational control of factor XII gene expression by IL-6. Downregulation of factor XII production by IL-6 in vitro parallelled that of transthyretin, a known negative acute-phase protein. Moreover, we show that, in patients developing an acute-phase response after immunotherapy with IL-2, plasma levels of factor XII correlate (r = .76, P < .0001) with those of transthyretin. Taken together, these results suggest that factor XII behaves as a negative acute-phase protein.

scholarly journals Interleukin-6 Downregulates Factor XII Production by Human Hepatoma Cell Line (HepG2)

Blood ◽  
1997 ◽  
Vol 90 (4) ◽  
pp. 1501-1507 ◽  
Author(s):  
Franca Citarella ◽  
Angelina Felici ◽  
Mieke Brouwer ◽  
John Wagstaff ◽  
Antonio Fantoni ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Factor Xii ◽  
Human Hepatoma Cell ◽  
Phase Protein ◽  
Hepatoma Cell Line Hepg2

Abstract Involvement of the contact system of coagulation in the pathogenesis of various inflammatory diseases is suggested by reduced plasma levels of factor XII (Hageman factor) and prekallikrein generally considered to result from activation of the contact system. However, in many of these diseases patients develop an acute-phase response and, therefore, an alternative explanation for the decreased levels of factor XII could be the downregulation of factor XII gene expression in the liver as described for negative acute-phase proteins. We report here that interleukin-6 (IL-6), the principal cytokine mediating the synthesis of most acute-phase proteins in the liver, downregulates the production of factor XII by the human hepatoma cell line HepG2 by up to 75%. The decrease in protein secretion correlated with an equivalent decrease of factor XII mRNA likely indicating a pretranslational control of factor XII gene expression by IL-6. Downregulation of factor XII production by IL-6 in vitro parallelled that of transthyretin, a known negative acute-phase protein. Moreover, we show that, in patients developing an acute-phase response after immunotherapy with IL-2, plasma levels of factor XII correlate (r = .76, P < .0001) with those of transthyretin. Taken together, these results suggest that factor XII behaves as a negative acute-phase protein.

Corticotrophin-releasing factor as a mediator of the acute-phase response in rats, mice and rabbits

1993 ◽  
Vol 136 (2) ◽  
pp. 207-216 ◽  
Author(s):  
P. M. Hagan ◽  
S. Poole ◽  
A. F. Bristow
Keyword(s):  
Protein Synthesis ◽  
Acute Phase ◽  
Acute Phase Response ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Phase Response ◽  
Interleukin 1Β ◽  
Corticotrophin Releasing Factor ◽  
Phase Protein

ABSTRACT The acute-phase response involves a number of separate physiological components, including induction of acute-phase protein synthesis by the liver. This response can be induced in vivo by administration of the endogenous leucocytic mediator interleukin-1β. A number of in-vivo effects of interleukin-1β have been reported to be mediated by corticotrophin-releasing factor (CRF), including activation of the hypothalamo-pituitary-adrenal axis and induction of fever, and in this report we have examined a possible involvement of CRF in mediating interleukin-1β-induced acute-phase protein synthesis. Interleukin-1β stimulated the elevation of species-specific plasma acute-phase proteins in rats, mice and rabbits. Co-injection of interleukin-1β with the specific CRF receptor antagonist α-helical-CRF9–41 NH2 abolished or attenuated acute-phase protein synthesis induced by interleukin-1β in all three species for up to 12 h after injection. The inhibitory effect of α-helical-CRF9–41NH2 was reduced or absent 24 h after injection. Neutralizing anti-CRF antisera had no effect on acute-phase protein synthesis in the mouse and, paradoxically, potentiated acute-phase protein synthesis induced by interleukin-1β in the rat. These results indicate a possible mediatory role for CRF in regulation of acute-phase protein synthesis, and suggest that CRF may mediate induction of acute-phase protein synthesis by a different mechanism from that involved in regulation of corticotrophin secretion. Journal of Endocrinology (1993) 136, 207–216

Assessment of pentraxin 3 in a systemic inflammatory response occurring with neonatal bacterial infection

2021 ◽  
pp. 1-6
Author(s):  
A. Sabry ◽  
M. Ibrahim ◽  
A. Khashana
Keyword(s):  
Inflammatory Response ◽  
Acute Phase ◽  
Neonatal Sepsis ◽  
Acute Phase Protein ◽  
Systemic Inflammatory Response ◽  
Control Group ◽  
Pentraxin 3 ◽  
Phase Protein ◽  
Serum Crp ◽  
And Control

INTRODUCTION: In the developing countries, neonatal sepsis is the most common complication in neonatal period. It is as a systemic inflammatory response because of infection. Laboratory indicators, do not have satisfactory sensitivity. Thus, early identification of sepsis is still needed. Because PTX3 may be a faster acute-phase protein that is not liver-dependent, it is probable that it is superior to traditional biomarkers for mirroring rapid inflammatory courses. METHODS: A prospective case control study design was used to determine the sensitivity of pentraxin 3 in the diagnosis of neonatal sepsis to allow early diagnostic tool. This study was carried out on neonatal ICU unit in Suez Canal University Hospital and the studied population were divided into two groups, including patients diagnosed with neonatal sepsis, based on clinical, laboratory and positive blood culture results, and control group RESULTS: The study found that there was statistically significant differences between both groups in serum CRP values in diseased and control group (Mean = 49.3±37.4 mg/L, 26.8±17.2 mg/L, p <  0.05), and pentraxin values in diseased and control group (Mean = 5.2±3.7 mg/L, 2.3±0.78 mg/L, p <  0.0001). In addition, there were statistically significant correlations between pentraxin and serum CRP concentrations (p <  0.05) in diseased group. ROC curve showed that serum CRP demonstrated good diagnostic accuracy in predicting neonatal sepsis AUC = 0.875 with sensitivity of 100% and specificity of 92.3%. CONCLUSION: Serum PTX3 may be a promising acute-phase protein for interpretation of affected newborns with symptoms and signs of sepsis.

scholarly journals Determination of selected acute phase proteins during the treatment of limb diseases in dairy cows

2008 ◽  
Vol 53 (No. 4) ◽  
pp. 173-183 ◽  
Author(s):  
P. Jawor ◽  
S. Steiner ◽  
T. Stefaniak ◽  
W. Baumgartner ◽  
A. Rzasa
Keyword(s):  
Treatment Group ◽  
Dairy Cows ◽  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Serum Amyloid ◽  
Blood Samples ◽  
Amyloid A ◽  
Phase Protein

The purpose of this study was to assess the diagnostic value of fibrinogen, haptoglobin, and serum amyloid A determination in the monitoring of the treatment of limb diseases in dairy cows. Fourteen lame cows were examined, while 10 clinically healthy cows constituted the control group. Blood samples from the ill animals were collected on three occasions: (1) upon arrival at the clinic, (2) between the third and sixth day after arriving, and (3) upon return to the owner. Blood samples from the control cows were collected once. Plasma levels of fibrinogen, haptoglobin, serum amyloid A, and total serum protein and its fractions (albumin, &alpha;-, &beta;-, &gamma;-globulins) were measured. Significantly higher fibrinogen, haptoglobin, and serum amyloid A levels were observed in the affected cows upon arrival at the clinic than in the control cows. Based on the changes in fibrinogen, haptoglobin, and serum amyloid A concentrations, the cows were divided into those with a systematic decrease in acute-phase protein levels during treatment (Group I, <I>n</I> = 6) and those which showed an increase in one or more acute-phase proteins despite treatment (Group II, <I>n</I> = 8). A stepwise decrease in the examined acute-phase proteins was observed in the first group and indicated an uncomplicated course of treatment; however, treatment of the second group did not appear to be wholly successful. A majority of the cows under treatment (<I>n</I> = 13) exhibited abnormal levels of the examined acute-phase proteins upon return to the owner. This indicates that these patients did not recover completely. The monitoring of plasma acute-phase protein concentrations can be a valuable complement to the clinical assessment of the treatment course and in the early detection of disease complications.

scholarly journals Evaluation of acute phase protein indexes in dogs with leishmaniasis at diagnosis, during and after short-term treatment

2012 ◽  
Vol 50 (No. 1) ◽  
pp. 39-46 ◽  
Author(s):  
S. Martinez-Subiela ◽  
Ceron JJ
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Acute Phase Protein ◽  
Term Treatment ◽  
Response To Therapy ◽  
Response To Treatment ◽  
Canine Leishmaniasis ◽  
Short Term Treatment ◽  
Phase Protein ◽  
Before And After

An acute phase index based on a combination of acute phase proteins which permitted monitoring the response to therapy of canine leishmaniasis was developed and evaluated in this study. Six dogs naturally infected by Leishmania infantum were treated with meglumine antimoniate (Glucantime<sup></sup>, Merial, Lyon, France) 100 mg/kg/day sc, given concurrently for 20 days with allopurinol (Zyloric&reg;, Glaxo Wellcome, Madrid, Spain) 30 mg/kg/day po and then, allopurinol alone for one month at the same dosage. Blood samples for acute phase proteins were obtained on different days before and after the beginning of treatment and two groups of indexes were calculated: (1) Indexes that combined one positive and one negative acute phase protein and (2) Indexes that combined two positive and one negative acute phase proteins. All calculated indexes were significantly higher in animals with leishmaniasis compared with clinically healthy dogs (n = 8) and a decrease was observed in all dogs tested during the treatment. Indexes that combined C-reactive protein (CRP) and ceruloplasmin (CP) with other proteins showed greater percentages of decrease that were statistically significant. Among these, the index CRP*CP/Alb was selected as the optimum since it showed a larger and faster decrease compared with the others as well as with individual proteins alone. These results would support the use of selected acute phase indexes, especially the CRP*CP/Alb index, to suspect about a leishmaniotic dogs and to monitor their response to treatment.

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