scholarly journals Comparison of maximal lactate steady state with V2, V4, individual anaerobic threshold and lactate minimum speed in horses

Author(s):  
O.A.B. Soares ◽  
G.C. Ferraz ◽  
C.B. Martins ◽  
D.P.M. Dias ◽  
J.C. Lacerda-Neto ◽  
...  

The anaerobic threshold is a physiologic event studied in various species. There are various methods for its assessment, recognized in the human and equine exercise physiology literature, several of these involving the relationship between blood lactate concentration (LAC) and exercise load, measured in a standardized exercise test. The aim of this study was to compare four of these methods: V2, V4, individual anaerobic threshold (IAT) and lactate minimum speed (LMS) with the method recognized as the gold standard for the assessment of anaerobic threshold, maximal lactate steady-state (MLSS). The five tests were carried out in thirteen trained Arabian horses, in which velocities and associated LAC could be measured. The mean velocities and the LAC associated with the anaerobic threshold for the five methods were respectively: V2 = 9.67±0.54; V4 = 10.98±0.47; V IAT = 9.81±0.72; V LMS = 7.50±0.57 and V MLSS = 6.14±0.45m.s-1 and LAC IAT = 2.17±0.93; LAC LMS = 1.17±0.62 and LAC MLSS = 0.84±0.21mmol.L-1. None of the velocities were statistically equivalent to V MLSS (P<0.05). V2, V4 and V LMS showed a good correlation with V MLSS , respectively: r = 0.74; r = 0.78 and r = 0.83, and V IAT did not significantly correlate with V MLSS. Concordance between the protocols was relatively poor, i.e., 3.28±1.00, 4.84±0.30 and 1.43±0.32m.s-1 in terms of bias and 95% agreement limits for V2, V4 and LMS methods when compared to MLSS. Only LAC LMS did not differ statistically from LAC MLSS. Various authors have reported the possibility of the assessment of anaerobic threshold using rapid protocols such as V4 and LMS for humans and horses. This study corroborates the use of these tests, but reveals that adjustments in the protocols are necessary to obtain a better concordance between the tests and the MLSS.

1996 ◽  
Vol 8 (4) ◽  
pp. 328-336 ◽  
Author(s):  
Ralph Beneke ◽  
Volker Schwarz ◽  
Renate Leithäuser ◽  
Matthias Hütler ◽  
Serge P. von Duvillard

Maximal lactate steady state (MLSS) corresponds to the prolonged constant workload whereby the kinetics of blood lactate concentration clearly increases from steady state. Different results of MLSS in children may reflect specific test protocols or definitions. Three methods corresponding to lactate time courses during 20 min (MLSS I), 16 min (MLSS II), and 8 min (MLSS III) of constant submaximal workload were intraindividually compared in 10 boys. At MLSS I, lactate, V̇O2peak, heart rate, and workload were higher (p < .05) than at MLSS II and at MLSS III. The differences between MLSS I, MLSS II, and MLSS III reflect insufficient contribution to lactate kinetics by testing procedures, strongly depending on the lactate time courses during the initial 10 min of constant workload. Previously published divergent results of MLSS in children seem to reflect a methodological effect more than a metabolic change.


2009 ◽  
Vol 21 (4) ◽  
pp. 493-505 ◽  
Author(s):  
Ralph Beneke ◽  
Hermann Heck ◽  
Helge Hebestreit ◽  
Renate M Leithäuser

The value of blood lactate concentration (BLC) measured during incremental load tests in predicting maximal lactate-steady-state (MLSS) workload has rarely been investigated in children. In 17 children and 18 adults MLSS was 4.1 ± 0.9mmol 1.1. Workload at BLC of 3.0mmol 1.1 determined during an incremental load test explained about 80% of the variance (p < .001) and best predicted MLSS workload independent of age. This was despite the increase in power per time related to maximum incremental load test power being higher (p < .001) in children than in adults. The BLC response to given exercise intensities is faster in children without affecting MLSS.


2018 ◽  
Vol 7 (1) ◽  
pp. 9-16
Author(s):  
Jose Ramon Lillo-Bevia ◽  
Ricardo Moran-Navarro ◽  
Alejandro Martinez-Cava ◽  
Victor Cerezuela ◽  
Jesus G. Pallares

The main aim of this study is to assess the validity of a new cycling protocol to estimate the Maximal Lactate Steady-State workload (MLSS) through a one-day incremental protocol (1day_MLSS). Eleven well-trained male cyclists performed 3 to 4 trials of 30-min constant load test (48-72h in between) to determine their respective MLSS workload. Then, on separate days, each cyclist carried out two identical graded exercise tests, comprised of four 10-minute long stages, with the initial load at 63% of their respective maximal aerobic power, 0.2 W·Kg-1 increments, and blood lactate concentration (BLC) determinations each 5 min. The results of the 1day_MLSS tests were analysed through three different constructs: i) BLC difference between 5th and 10th min of each stage (DIF_5to10), ii) BLC difference between the 10th min of two consecutive stages (DIF_10to10), and iii) difference in the mean BLC between the 5th and 10th min of two consecutive stages (DIF_mean). For all constructs, the physiological steady state was determined as the highest workload that could be maintained with a BLC rise lower than 1mmol·L-1.  No significant differences were detected between the MLSS workload (247 ± 22W) and any of the 1day_MLSS data analysis (250 ± 24W, 245 ± 23W and 243 ± 21W, respectively; p>0.05). When compared to the MLSS workload, strong ICCs and low bias values were found for these three constructs, especially for the DIF_10to10 workload (r=0.960; Bias=2.2 W). High within-subject reliability data were found for the DIF10_10 construct (ICC=0.846; CV=0.4%; Bias=2.2 ± 6.4W). The 1day_MLSS test and DIF_10to10 data analysis is a valid assessment to predict the MLSS workload in cycling, that considerably reduces the dedicated time, effort and human resources that requires the original test. The validity and reliability values reported in this project are higher than those achieved by other previous MLSS estimation tests.


Sports ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 25 ◽  
Author(s):  
Ioannis Nikitakis ◽  
Giorgos Paradisis ◽  
Gregory Bogdanis ◽  
Argyris Toubekis

Background: The purpose of this study was to compare physiological responses during continuous and intermittent swimming at intensity corresponding to critical speed (CS: slope of the distance vs. time relationship using 200 and 400-m tests) with maximal lactate steady state (MLSS) in children and adolescents. Methods: CS and the speed corresponding to MLSS (sMLSS) were calculated in ten male children (11.5 ± 0.4 years) and ten adolescents (15.8 ± 0.7 years). Blood lactate concentration (BL), oxygen uptake ( V · O2), and heart rate (HR) at sMLSS were compared to intermittent (10 × 200-m) and continuous swimming corresponding to CS. Results: CS was similar to sMLSS in children (1.092 ± 0.071 vs. 1.083 ± 0.065 m·s−1; p = 0.12) and adolescents (1.315 ± 0.068 vs. 1.297 ± 0.056 m·s−1; p = 0.12). However, not all swimmers were able to complete 30 min at CS and BL was higher at the end of continuous swimming at CS compared to sMLSS (children: CS: 4.0 ± 1.8, sMLSS: 3.4 ± 1.5; adolescents: CS: 4.5 ± 2.3, sMLSS: 3.1 ± 0.8 mmol·L−1; p < 0.05). V · O2 and HR in continuous swimming at CS were not different compared to sMLSS (p > 0.05). BL, V · O2 and HR in 10 × 200-m were similar to sMLSS and no different between groups. Conclusion: Intermittent swimming at CS presents physiological responses similar to sMLSS. Metabolic responses of continuous swimming at CS may not correspond to MLSS in some children and adolescent swimmers.


2002 ◽  
Vol 27 (3) ◽  
pp. 232-249 ◽  
Author(s):  
Brian R. Macintosh ◽  
Shane Esau ◽  
Krista Svedahl

This study evaluated the reliability and validity of the lactate minimum test (LMT), an incremental test given after lactic acidosis wax induced by sprint exercise. This test is purported to accurately estimate the intensity of exercise at which the transport of lactate into and out of the blood is in equilibrium (maximal lactate steady state or MLSS) and should be a good predictor of endurance performance. Fourteen athletes (mean age 27.2 ± 3.7 yrs) completed the following on Kreitler rollers: (a) two 20-km time-trials (35.1 ± 3.3 and 35.7 ± 3.5 km • hr−1, p < .05); (b) two LMTs yielding lactate minimum speeds (LMS) of 33.6 ± 3.4 and 33.4 ± 3.1 km • hr−1 (p > 0.6); and (c) four constant intensity rides, at speeds bracketing the LMS. At 33.5 ± 3.1 km • hr−1 plasma lactate concentration decreased 0.4 ± 1.6 mM from 10 to 30 min. Plasma lactate increased 1.6 ± 0.7 mM white riding 0.9 ± 0.9 km • hr−1 faster. The LMT is a reliable (r2 = 0.904) and valid method to predict MLSS and a good predictor of endurance performance (LMT vs. 20-km time-trial, r2 = 0.86). Key words: anaerobic threshold, endurance, blood lactate


2007 ◽  
Vol 32 (6) ◽  
pp. 1058-1064 ◽  
Author(s):  
Arie Rotstein ◽  
Raffy Dotan ◽  
Levana Zigel ◽  
Tally Greenberg ◽  
Yael Benyamini ◽  
...  

The purpose of this study was to investigate the effect of pre-test carbohydrate (CHO) ingestion on anaerobic-threshold assessment using the lactate-minimum test (LMT). Fifteen competitive male distance runners capable of running 10 km in 33.5–43 min were used as subjects. LMT was performed following CHO (2 × 300 mL, 7% solution) or comparable placebo (Pl) ingestion, in a double-blind, randomized order. The LMT consisted of two high-intensity 1 min treadmill runs (17–21 km·h–1), followed by an 8 min recovery period. Subsequently, subjects performed 5 min running stages, incremented by 0.6 km·h–1 and separated by 1 min blood-sampling intervals. Tests were terminated after 3 consecutive increases in blood-lactate concentration ([La]) had been observed. Finger-tip capillary blood was sampled for [La] and blood-glucose determination 30 min before the test’s onset, during the recovery phase following the 2 high-intensity runs, and following each of the subsequent 5 min stages. Heart rate (HR) and rating of perceived exertion (RPE) were recorded after each stage. The lactate-minimum speed (LMS) was determined from the individual [La]–velocity plots and was considered reflective of the anaerobic threshold. Pre-test CHO ingestion had no effect on LMS (13.19 ± 1.12 km·h–1 vs. 13.17 ± 1.08 km·h–1 in CHO and Pl, respectively), nor on [La] and glucose concentration at that speed, or on HR and RPE responses. Pre-test CHO ingestion therefore does not affect LMS or the LMT-estimated anaerobic threshold.


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