scholarly journals Jaundice in second intention wound healing in rats

2004 ◽  
Vol 19 (3) ◽  
pp. 176-185
Author(s):  
Maria de Lourdes Pessole Biondo-Simões ◽  
Celso Fernando Ribeiro Araújo ◽  
João Henrique Felício de Lima ◽  
Marcelo Ferreira ◽  
Marcos de Abreu Bonardi
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Biochemical Changes ◽  
Male Rats ◽  
Second Step ◽  
Collagen Concentration ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Histopathologic Analysis ◽  
The Ideal

PURPOSE: The objective of the present study was to monitor the process of second intention skin wound healing in rats with jaundice. METHODS: The study was divided into two steps. In the first, obstructive jaundice was induced in 68 male rats to investigate the biochemical changes and to determine the ideal time for the study of healing. In the second step, 54 rats were divided into two groups: control (normal) and experimental (jaundiced). Seventy-two hours after jaundice induction, a standardized wound was produced in the dorsum by removing the skin and the subcutaneous net, whose contraction was assessed on the 3rd, 7th, 10th and 14th day and monitored with a computer program. After sacrifice, the tissue was submitted to histopathologic analysis. RESULTS: The most marked biochemical changes were detected between the 3rd and 5th day. The reduction of he wound area was slower in the jaundice group at all 4 time points studied (p3=0.0366, p7=0.0054, p10=0.0000. p14=0.0000). Collagen concentration was lower in the wound of jaundiced animals (p3=0.0000. p7=0.0000. p14=0.0000). CONCLUSION: Jaundice delays collagen deposition and maturation and wound contraction in wounds left to heal by second intention wound healing in rats.

scholarly journals Expression of VEGF and TGF-β Genes in Skin Wound Healing Process Induced Using Phenytoin in Male Rats

2019 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Roghaye Savari ◽  
Mohammad Shafiei ◽  
Hamid Galehdari ◽  
Mahnaz Kesmati
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Skin Wound ◽  
Male Rats ◽  
Wound Healing Process ◽  
Skin Wound Healing

scholarly journals Phytochemical Composition, Antioxidant Activity, and the Effect of the Aqueous Extract of Coffee (Coffea arabica L.) Bean Residual Press Cake on the Skin Wound Healing

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Regina Celis Lopes Affonso ◽  
Ana Paula Lorenzen Voytena ◽  
Simone Fanan ◽  
Heloísa Pitz ◽  
Daniela Sousa Coelho ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Press Cake ◽  
Negative Control ◽  
Skin Wound ◽  
Coffee Bean ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Inflammatory Phase ◽  
Coffee Beans

The world coffee consumption has been growing for its appreciated taste and its beneficial effects on health. The residual biomass of coffee, originated in the food industry after oil extraction from coffee beans, called coffee beans residual press cake, has attracted interest as a source of compounds with antioxidant activity. This study investigated the chemical composition of aqueous extracts of coffee beans residual press cake (AE), their antioxidant activity, and the effect of topical application on the skin wound healing, in animal model, of hydrogels containing the AE, chlorogenic acid (CGA), allantoin (positive control), and carbopol (negative control). The treatments’ performance was compared by measuring the reduction of the wound area, with superior result (p<0.05) for the green coffee AE (78.20%) with respect to roasted coffee AE (53.71%), allantoin (70.83%), and carbopol (23.56%). CGA hydrogels reduced significantly the wound area size on the inflammatory phase, which may be associated with the well known antioxidant and anti-inflammatory actions of that compound. The topic use of the coffee AE studied improved the skin wound healing and points to an interesting biotechnological application of the coffee bean residual press cake.

Wound healing effect benzosulfonate 1-ethyl-3-methyl-4,5-bis(n-methylcarbamoyl) imidazolium

2020 ◽  
Vol 18 (3) ◽  
pp. 229-235
Author(s):  
Lydmila K. Khnychenko ◽  
Elena N. Selina ◽  
Olga M. Rodionova ◽  
Levon B. Piotrovskiy ◽  
Petr D. Shabanov
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Experimental Models ◽  
Skin Wound ◽  
Complete Healing ◽  
Male Rats ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Wound Healing Effect

Materials and methods. In experiments on 180 male rats weighing 180200 g, the wound-healing effect of derivatives of imidazole (IEM-1181) was evaluated as a 10% ointment on models of aseptic full-layer linear and planar skin wounds. Results. It was found that the compound IEM-1181 in the form of 10% ointment has a pronounced on skin wound healing effect, manifested in the qualitative features of the regenerative healing process. With local application of the ointment containing the tested compound, the strength of the tissue formed on the site of a full-layer linear skin wound was increased when its edges were stretched, and the healing time of full-layer planar wounds was reduced. The morphological picture of the tissue formed at the site of the wound defect corresponded to complete healing by primary tension with complete epithelization of the surface and squamous cell differentiation of the epithelial regenerate without signs of inflammation and scar formation. Conclusion. The results of the study on experimental models of full-layer linear and planar skin wounds indicate that the wound healing effect of the tested ointment is due to the anti-inflammatory activity of the IEM-1181 compound

scholarly journals The Combined Effect of Photobiomodulation and Curcumin on Acute Skin Wound Healing in Rats

2021 ◽  
Vol 12 ◽  
pp. e9-e9
Author(s):  
Abdollah Amini ◽  
Hasan Soleimani ◽  
Fatemehalsadat Rezaei ◽  
Seyed Kamran Ghoreishi ◽  
Sufan Chien ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Skin Wound ◽  
Control Group ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Group 4 ◽  
Patient Morbidity ◽  
Group 3 ◽  
Combined Impact

Introduction: Abnormal wound repair is a cause for considerable expense, as well as patient morbidity and mortality. Here, we investigated the combined impact of photobiomodulation (PBM) and curcumin on a rat experimental model of an acute skin wound. Methods: A round full-thickness wound was created on the back of each rat. We divided the rats into the following four groups. Group one was the control group. Group two received pulse wave (PW) PBM at a dose of 890 nm, 80 Hz, and 0.2 J/cm2 . Group 3 received 40 mg/kg curcumin by gastric gavage and group 4 were treated with PWPBM + curcumin. We measured the wound area on days 4, 7, and 15, and performed microbiological and tensiometric examinations. Results: There was markedly improved wound contraction in the curcumin (7.5 ± 0.57; P=0.000), PBM (8.5 ± 1.2; P=0.000), and PBM + curcumin (14.5 ± 4.3; P=0.002) groups relative to the control group (25 ± 6). PBM (100 ± 7.3; P=0.005), and PBM + curcumin (98 ± 6; P=0.005) groups meaningfully improved tensile strength relative to the control group (61 ± 8.2). On day 15, the PBM (10 ± 5; P=0.000), curcumin (14 ± 4.5, P=0.000), and PBM + curcumin (27.3 ± 8.3; P=0.000) groups meaningfully decreased microbial flora relative to the control group (95 ± 6). Conclusion: We concluded that the PBM and PBM + curcumin groups meaningfully accelerated wound healing of the acute skin wound in the rats. The results of the PBM group were statistically more effective than the curcumin alone and PBM + curcumin-treated groups.

scholarly journals Dinamics of skin wound healing with the use of injectable regeneration stimulators in rats

2020 ◽  
pp. 54-63
Author(s):  
Е.В. Силина ◽  
Н.Е. Мантурова ◽  
Е.Б. Артюшкова ◽  
П.Ф. Литвицкий ◽  
В.И. Васин ◽  
...  
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Subsequent Treatment ◽  
Male Rats ◽  
Control Group ◽  
Skin Wound Healing ◽  
Vascular Effect ◽  
Regeneration Time ◽  
Collagen Group ◽  
Treatment Of Wounds

Цель исследования - выявление особенностей динамики заживления кожной раны у экспериментальных крыс зрелого возраста; сравнение эффективности зарегистрированных и применяющихся в клинической практике инъекционных стимуляторов регенерации тканей: депротеинизированного гемодеривата крови телят и коллагена. Методика. Экспериментальное слепое исследование проведено на 9-месячных крысах самцах Wistar массой 443,6±6,5 г. Квадратную рану стандартного размера (каждая сторона 11 мм, глубина раны до фасции, площадь в среднем 123,5±2,1 мм2) наносили на поверхность спины животных. В течение 14 сут изучали эффективность и возможные механизмы действия на заживление кожных ран двух инъекционных лекарственных средств: депротеинизированного гемодеривата крови телят (ДГК, актовегин) и коллагена (Коллост) в сравнении с контролем (физиологический раствор натрия хлорида). В динамике оценивали изменение площади ран, их объема, состояния микрогемоциркуляции. Результаты. Площадь ран группы Контроль сократились к концу 7-х сут в 1,1 раза, 14-х - в 5,0 раза, группы ДГК - в 1,4 и 8,3 раза, группы Коллаген - в 1,5 и 11,9 раза соответственно. В группах Контроль и ДГК заживление ран проходило в горизонтальном направлении, в группе Коллаген - вертикальном. Увеличение площади ран в фазу острого воспаления (в первые 3-5 сут) было только в группах Контроль и ДГК, при которых оно было перпендикулярно линиям напряжения кожи. Это свидетельствует о значимости направлений хирургических разрезов и наложения швов не только при создании, но и при последующем лечении ран. Уровень микроциркуляции в коже, непосредственно примыкающей к краю раны, на 3-и сут увеличивался только в группе ДГК, на 7-е сут - в группе Коллаген, на 14-е сут - во всех ранах всех групп, но наибольшие изменения микрогемоперфузии были в группе Коллаген. Заключение. Установлена эффективность обоих стимуляторов регенерации. Вместе с тем скорость и характер заживления ран при их применении существенно различались, что связано с разными механизмами действия препаратов. Это необходимо учитывать в клинической практике при подборе препаратов для ускорения сроков регенерации раны, уменьшения размеров послеоперационного рубца и повышения его эстетичности. Aim. To characterize the dynamics of skin wound healing in mature experimental rats and to compare the effectiveness of approved clinical injectable stimulants of regeneration (deproteinized hemoderivative of calf blood and collagen). Methods. This experimental blind study was conducted on 9-month-old Wistar male rats weighing 443.6±6.5 g. Square wounds of a standardized size (each side, 11 mm; wound depth, down to fascia; mean area, 123.5 ± 2.1 mm2) were inflicted on the rat back. Efficacy and mechanism of action of two injectable drugs were studied for 14 days. Effects of deproteinized hemoderivative of calf blood (DHB, Actovegin) and collagen (Collost) were compared with control wounds (saline treatment). The wound size and volume and the state of microcirculation were evaluated in dynamics. Results. The area of the wounds decreased by 9.1% at 7 days and 80% at 14 days in the control group; by 28.6% and 88%, respectively, in the DHB group; and by 33.3% and 91.6%, respectively, in the collagen group. In the control and DHA groups, the wound healing was predominantly horizontal while in the collagen group is was vertical. An increase in the wound area in the phase of acute inflammation during the first 3-5 days was observed only in the control and DHB groups; this increase was localized perpendicular to skin tension lines. This indicates the importance of preoperative planning not only for the lines and directions of surgical incisions and suturing but also for the subsequent treatment of wounds. Microcirculation in the skin immediately adjacent to the wound edge increased on day 3 only in the DHB group; in the collagen group, it increased on day 7; and on day 14, it was increased in all wounds of all groups with the greatest vascular effect being observed in the collagen group. Conclusion. Both regeneration stimulants were effective; however, the wound healing patterns and rates were different, which might be due to different mechanisms of action of the drugs. This fact should be taken into account in clinical practice in order to reduce the regeneration time as well as to reduce the size and aesthetics of postoperative scar.

scholarly journals Histological Study of the First Seven Days of Skin Wound Healing in Rats

2006 ◽  
Vol 75 (2) ◽  
pp. 197-202 ◽  
Author(s):  
B. Vidinský ◽  
P. Gál ◽  
T. Toporcer ◽  
F. Longauer ◽  
Ľ. Lenhardt ◽  
...  
Keyword(s):  
Wound Healing ◽  
Histological Study ◽  
Healing Process ◽  
Skin Wound ◽  
Male Rats ◽  
Histological Evaluation ◽  
Wound Healing Process ◽  
Physical Factors ◽  
Skin Wound Healing ◽  
Inflammatory Phase

The aim of this study was to elaborate a histological model of incisional skin wound healing in Sprague-Dawley rats. Under aseptic conditions two paravertebral full thickness skin incisions were performed on the back of 42 anesthetized male rats. Histological sections from tissue specimens were stained by hematoxylin and eosin, van Gieson, PAS + PSD, Mallory's phosphotungstic hematoxylin and azur and eosin and evaluated during the first seven days after surgery. Histological evaluation revealed that the regeneration of injured epidermis was completed five days after surgery. The inflammatory phase was recorded during the first three days of healing with the culmination of this phase between day one and day two. The beginning of the proliferative phase was dated to the first day and the peak during day five and day six. The initiation of the maturation and remodeling phase of the healing process was observed six days after wounding. At the layer of striated muscle, the centronucleated cells were described for the first time six days after surgery. The wound healing process of rat skin was histologically described during the first seven days. Results of this work can serve as an experimental model for further research using external pharmacological and physical factors (laser light, magnetic field) by which the wound healing can be favourably influenced.

scholarly journals Acceleration of skin wound healing by low-dose indirect ionizing radiation in male rats

2017 ◽  
Vol 33 (8) ◽  
pp. 385-393 ◽  
Author(s):  
Nasrollah Jabbari ◽  
Gholam Hossein Farjah ◽  
Behnam Ghadimi ◽  
Hajar Zanjani ◽  
Behnam Heshmatian
Keyword(s):  
Wound Healing ◽  
Ionizing Radiation ◽  
Low Dose ◽  
Skin Wound ◽  
Male Rats ◽  
Skin Wound Healing

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

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