scholarly journals Phytochemical Composition, Antioxidant Activity, and the Effect of the Aqueous Extract of Coffee (Coffea arabica L.) Bean Residual Press Cake on the Skin Wound Healing

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Regina Celis Lopes Affonso ◽  
Ana Paula Lorenzen Voytena ◽  
Simone Fanan ◽  
Heloísa Pitz ◽  
Daniela Sousa Coelho ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Wound Healing ◽  
Press Cake ◽  
Negative Control ◽  
Skin Wound ◽  
Coffee Bean ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Inflammatory Phase ◽  
Coffee Beans

The world coffee consumption has been growing for its appreciated taste and its beneficial effects on health. The residual biomass of coffee, originated in the food industry after oil extraction from coffee beans, called coffee beans residual press cake, has attracted interest as a source of compounds with antioxidant activity. This study investigated the chemical composition of aqueous extracts of coffee beans residual press cake (AE), their antioxidant activity, and the effect of topical application on the skin wound healing, in animal model, of hydrogels containing the AE, chlorogenic acid (CGA), allantoin (positive control), and carbopol (negative control). The treatments’ performance was compared by measuring the reduction of the wound area, with superior result (p<0.05) for the green coffee AE (78.20%) with respect to roasted coffee AE (53.71%), allantoin (70.83%), and carbopol (23.56%). CGA hydrogels reduced significantly the wound area size on the inflammatory phase, which may be associated with the well known antioxidant and anti-inflammatory actions of that compound. The topic use of the coffee AE studied improved the skin wound healing and points to an interesting biotechnological application of the coffee bean residual press cake.

scholarly journals Jaundice in second intention wound healing in rats

2004 ◽  
Vol 19 (3) ◽  
pp. 176-185
Author(s):  
Maria de Lourdes Pessole Biondo-Simões ◽  
Celso Fernando Ribeiro Araújo ◽  
João Henrique Felício de Lima ◽  
Marcelo Ferreira ◽  
Marcos de Abreu Bonardi
Keyword(s):  
Wound Healing ◽  
Skin Wound ◽  
Biochemical Changes ◽  
Male Rats ◽  
Second Step ◽  
Collagen Concentration ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Histopathologic Analysis ◽  
The Ideal

PURPOSE: The objective of the present study was to monitor the process of second intention skin wound healing in rats with jaundice. METHODS: The study was divided into two steps. In the first, obstructive jaundice was induced in 68 male rats to investigate the biochemical changes and to determine the ideal time for the study of healing. In the second step, 54 rats were divided into two groups: control (normal) and experimental (jaundiced). Seventy-two hours after jaundice induction, a standardized wound was produced in the dorsum by removing the skin and the subcutaneous net, whose contraction was assessed on the 3rd, 7th, 10th and 14th day and monitored with a computer program. After sacrifice, the tissue was submitted to histopathologic analysis. RESULTS: The most marked biochemical changes were detected between the 3rd and 5th day. The reduction of he wound area was slower in the jaundice group at all 4 time points studied (p3=0.0366, p7=0.0054, p10=0.0000. p14=0.0000). Collagen concentration was lower in the wound of jaundiced animals (p3=0.0000. p7=0.0000. p14=0.0000). CONCLUSION: Jaundice delays collagen deposition and maturation and wound contraction in wounds left to heal by second intention wound healing in rats.

Roles of lactoferrin on skin wound healing1This article is part of Special Issue entitled Lactoferrin and has undergone the Journal's usual peer review process.

2012 ◽  
Vol 90 (3) ◽  
pp. 497-503 ◽  
Author(s):  
Yoshiharu Takayama ◽  
Reiji Aoki
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Chronic Wounds ◽  
Peer Review Process ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Inflammatory Phase ◽  
Complex Biological Process ◽  
Extracellular Matrix Components

Skin wound healing is a complex biological process that requires the regulation of different cell types, including immune cells, keratinocytes, fibroblasts, and endothelial cells. It consists of 5 stages: hemostasis, inflammation, granulation tissue formation, re-epithelialization, and wound remodeling. While inflammation is essential for successful wound healing, prolonged or excess inflammation can result in nonhealing chronic wounds. Lactoferrin, an iron-binding glycoprotein secreted from glandular epithelial cells into body fluids, promotes skin wound healing by enhancing the initial inflammatory phase. Lactoferrin also exhibits anti-inflammatory activity that neutralizes overabundant immune response. Accumulating evidence suggests that lactoferrin directly promotes both the formation of granulation tissue and re-epithelialization. Lactoferrin stimulates the proliferation and migration of fibroblasts and keratinocytes and enhances the synthesis of extracellular matrix components, such as collagen and hyaluronan. In an in vitro model of wound contraction, lactoferrin promoted fibroblast-mediated collagen gel contraction. These observations indicate that lactoferrin supports multiple biological processes involved in wound healing.

scholarly journals The Combined Effect of Photobiomodulation and Curcumin on Acute Skin Wound Healing in Rats

2021 ◽  
Vol 12 ◽  
pp. e9-e9
Author(s):  
Abdollah Amini ◽  
Hasan Soleimani ◽  
Fatemehalsadat Rezaei ◽  
Seyed Kamran Ghoreishi ◽  
Sufan Chien ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Skin Wound ◽  
Control Group ◽  
Skin Wound Healing ◽  
Wound Area ◽  
Group 4 ◽  
Patient Morbidity ◽  
Group 3 ◽  
Combined Impact

Introduction: Abnormal wound repair is a cause for considerable expense, as well as patient morbidity and mortality. Here, we investigated the combined impact of photobiomodulation (PBM) and curcumin on a rat experimental model of an acute skin wound. Methods: A round full-thickness wound was created on the back of each rat. We divided the rats into the following four groups. Group one was the control group. Group two received pulse wave (PW) PBM at a dose of 890 nm, 80 Hz, and 0.2 J/cm2 . Group 3 received 40 mg/kg curcumin by gastric gavage and group 4 were treated with PWPBM + curcumin. We measured the wound area on days 4, 7, and 15, and performed microbiological and tensiometric examinations. Results: There was markedly improved wound contraction in the curcumin (7.5 ± 0.57; P=0.000), PBM (8.5 ± 1.2; P=0.000), and PBM + curcumin (14.5 ± 4.3; P=0.002) groups relative to the control group (25 ± 6). PBM (100 ± 7.3; P=0.005), and PBM + curcumin (98 ± 6; P=0.005) groups meaningfully improved tensile strength relative to the control group (61 ± 8.2). On day 15, the PBM (10 ± 5; P=0.000), curcumin (14 ± 4.5, P=0.000), and PBM + curcumin (27.3 ± 8.3; P=0.000) groups meaningfully decreased microbial flora relative to the control group (95 ± 6). Conclusion: We concluded that the PBM and PBM + curcumin groups meaningfully accelerated wound healing of the acute skin wound in the rats. The results of the PBM group were statistically more effective than the curcumin alone and PBM + curcumin-treated groups.

scholarly journals Effect of gallium arsenide low-level laser therapy on the inflammatory phase of skin wound healing in rats

2017 ◽  
Vol 11 (4) ◽  
pp. 226-230
Author(s):  
Armando de Mattos Carvalho ◽  
Michele Henrique Antônio Bizo ◽  
Hugo Shisei Toma ◽  
Karla Moraes Rocha Guedes ◽  
Lívia Saab Muraro ◽  
...  
Keyword(s):  
Wound Healing ◽  
Gallium Arsenide ◽  
Laser Therapy ◽  
Skin Wound ◽  
Low Level Laser Therapy ◽  
Skin Wound Healing ◽  
Low Level ◽  
Low Level Laser ◽  
Inflammatory Phase ◽  
Level Laser

scholarly journals Evaluation of Small Molecular Polypeptides from the Mantle of Pinctada Martensii on Promoting Skin Wound Healing in Mice

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4231 ◽  
Author(s):  
Faming Yang ◽  
Xiaoming Qin ◽  
Ting Zhang ◽  
Haisheng Lin ◽  
Chaohua Zhang
Keyword(s):  
Amino Acids ◽  
Wound Healing ◽  
Transforming Growth Factor ◽  
Skin Wound ◽  
Inflammatory Factor ◽  
Bioactive Substances ◽  
Skin Wound Healing ◽  
Proliferative Phase ◽  
Pinctada Martensii ◽  
Inflammatory Phase

Skin wound healing, especially chronic wound healing, is a common challenging clinical problem. It is urgent to broaden the sources of bioactive substances that can safely and efficiently promote skin wound healing. This study aimed to observe the effects of small molecular peptides (SMPs) of the mantle of Pinctada martensii on wound healing. After physicochemical analysis of amino acids and mass spectrometry of SMPs, the effect of SMPs on promoting healing was studied through a whole cortex wound model on the back of mice for 18 consecutive days. The results showed that SMPs consisted of polypeptides with a molecular weight of 302.17–2936.43 Da. The content of polypeptides containing 2–15 amino acids accounted for 73.87%, and the hydrophobic amino acids accounted for 56.51%. Results of in vitro experimentation showed that SMPs possess a procoagulant effect, but no antibacterial activity. Results of in vivo experiments indicated that SMPs inhibit inflammatory response by secretion of anti-inflammatory factor IL-10 during the inflammatory phase; during the proliferative phase, SMPs promote the proliferation of fibroblasts and keratinocytes. The secretion of transforming growth factor-β1 and cyclin D1 accelerates the epithelialization and contraction of wounds. In the proliferative phase, SMPs effectively promote collagen deposition and partially inhibit superficial scar hyperplasia. These results show that SMPs promotes dermal wound healing in mice and have a tremendous potential for development and utilization in skin wound healing.

scholarly journals Histological Study of the First Seven Days of Skin Wound Healing in Rats

2006 ◽  
Vol 75 (2) ◽  
pp. 197-202 ◽  
Author(s):  
B. Vidinský ◽  
P. Gál ◽  
T. Toporcer ◽  
F. Longauer ◽  
Ľ. Lenhardt ◽  
...  
Keyword(s):  
Wound Healing ◽  
Histological Study ◽  
Healing Process ◽  
Skin Wound ◽  
Male Rats ◽  
Histological Evaluation ◽  
Wound Healing Process ◽  
Physical Factors ◽  
Skin Wound Healing ◽  
Inflammatory Phase

The aim of this study was to elaborate a histological model of incisional skin wound healing in Sprague-Dawley rats. Under aseptic conditions two paravertebral full thickness skin incisions were performed on the back of 42 anesthetized male rats. Histological sections from tissue specimens were stained by hematoxylin and eosin, van Gieson, PAS + PSD, Mallory's phosphotungstic hematoxylin and azur and eosin and evaluated during the first seven days after surgery. Histological evaluation revealed that the regeneration of injured epidermis was completed five days after surgery. The inflammatory phase was recorded during the first three days of healing with the culmination of this phase between day one and day two. The beginning of the proliferative phase was dated to the first day and the peak during day five and day six. The initiation of the maturation and remodeling phase of the healing process was observed six days after wounding. At the layer of striated muscle, the centronucleated cells were described for the first time six days after surgery. The wound healing process of rat skin was histologically described during the first seven days. Results of this work can serve as an experimental model for further research using external pharmacological and physical factors (laser light, magnetic field) by which the wound healing can be favourably influenced.

miR-29b promotes skin wound healing and reduces excessive scar formation by inhibition of the TGF-β1/Smad/CTGF signaling pathway

2017 ◽  
Vol 95 (4) ◽  
pp. 437-442 ◽  
Author(s):  
Jingdong Guo ◽  
Quan Lin ◽  
Ying Shao ◽  
Li Rong ◽  
Duo Zhang
Keyword(s):  
Wound Healing ◽  
Signaling Pathway ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Skin Wound ◽  
Scar Formation ◽  
Skin Wound Healing ◽  
Morphologic Alteration ◽  
Great Pain ◽  
Tgf Β1

The hypertrophic scar is a medical difficulty of humans, which has caused great pain to patients. Here, we investigated the inhibitory effect of miR-29b on scar formation. The scalded model was established in mice and miR-29b mimics or a negative control was subcutaneously injected into the injury skin. Then various molecular biological experiments were performed to assess the effect of miR-29b on scar formation. According to our present study, first, the results demonstrated that miR-29b was down-regulated in thermal injury tissue and miR-29b treatment could promote wound healing, inhibit scar formation, and alleviate histopathological morphologic alteration in scald tissues. Additionally, miR-29b treatment suppressed collagen deposition and fibrotic gene expression in scar tissues. Finally, we found that miR-29b treatment inhibited the TGF-β1/Smad/CTGF signaling pathway. Taken together, our data suggest that miR-29b treatment has an inhibitory effect against scar formation via inhibition of the TGF-β1/Smad/CTGF signaling pathway and may provide a potential molecular basis for future treatments for hypertrophic scars.

scholarly journals The Use of Menthol in Skin Wound Healing—Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization

Pharmaceutics ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1902
Author(s):  
Ariane Leite Rozza ◽  
Fernando Pereira Beserra ◽  
Ana Júlia Vieira ◽  
Eduardo Oliveira de Souza ◽  
Carlos Alberto Hussni ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
Cellular Proliferation ◽  
Antioxidant Defense System ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Ki 67 ◽  
Inflammatory Phase ◽  
Tnf Α

Wound healing involves inflammatory, proliferative, and remodeling phases, in which various cells and chemical intermediates are involved. This study aimed to investigate the skin wound healing potential of menthol, as well as the mechanisms involved in its effect, after 3, 7, or 14 days of treatment, according to the phases of wound healing. Skin wound was performed in the back of Wistar rats, which were topically treated with vehicle cream; collagenase-based cream (1.2 U/g); or menthol-based cream at 0.25%, 0.5%, or 1.0% over 3, 7, or 14 days. Menthol cream at 0.5% accelerated the healing right from the inflammatory phase (3 days) by decreasing mRNA expression of inflammatory cytokines TNF-α and Il-6. At the proliferative phase (7 days), menthol 0.5% increased the activity of antioxidant enzymes SOD, GR, and GPx, as well as the level of GSH, in addition to decreasing the levels of inflammatory cytokines TNF-α, IL-6, and IL-1β and augmenting mRNA expression for Ki-67, a marker of cellular proliferation. At the remodeling phase (14 days), levels of inflammatory cytokines were decreased, and the level of Il-10 and its mRNA expression were increased in the menthol 0.5% group. Menthol presented skin wound healing activity by modulating the antioxidant system of the cells and the inflammatory response, in addition to stimulating epithelialization.

scholarly journals The role of miRNAs in the inflammatory phase of skin wound healing

2021 ◽  
Vol 5 (4) ◽  
pp. 264-278
Author(s):  
Federica Serra ◽  
◽  
Lisa Aielli ◽  
Erica Costantini ◽  
◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Chronic Wounds ◽  
Medical Problem ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Damage Site ◽  
Inflammatory Phase ◽  
Peripheral Cells

<abstract> <p>Wound healing (WH) is a fundamental physiological process to keep the integrity of the skin, therefore impaired and chronic WH is a common and severe medical problem and represent one of the biggest challenges of public health. The resolution of the WH inflammatory phase is characterized by a complex series of events that involves many cellular types, especially neutrophils, macrophages and inflammatory mediators, which are crucial for a correct wound closure. MicroRNAs (miRNAs) play essential roles in wound repair. In fact, miR-142 is linked to inflammation modulating neutrophils' chemotaxis and polarization, while the polarization of M1 toward the M2 phenotype is driven by miR-223 and miR-132 is linked to chemokines and cytokines that activate endothelial cells and attract leukocytes and peripheral cells to the damage site. Thus, understanding the dysregulation of miRNAs in WH will be decisive for the development of new and more effective therapies for the management of chronic wounds.</p> </abstract>

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