scholarly journals Qualitative and quantitative analysis of rabbit's fat mesenchymal stem cells

2010 ◽  
Vol 25 (1) ◽  
pp. 24-27 ◽  
Author(s):  
Marcelo Paulo Vaccari Mazzetti ◽  
Isis Sousa Oliveira ◽  
Regiane Miranda-Ferreira ◽  
Grasiele Fauaz ◽  
Chaibe Nunes Ribeiro ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Mesenchymal Stem Cells ◽  
New Zealand ◽  
Quantitative Analysis ◽  
Culture Medium ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Satisfactory Method ◽  
New Zealand Rabbits

PURPOSE: To present an experimental model of qualitative and quantitative analysis of mesenchymal stem cells from fat of rabbits obtained by lipectomy. The fat could be a great source for obtaining mesenchymal stem cells and to create conditions for repairing injured tissues by bioengineering. METHODS: New Zealand rabbits (n= 10) adipose panicle (2-3 cm) were removed by lipectomy, fragmented and washed with PBS and enzymatically dissociated with trypsin/EDTA. Lately, these cells were incubated in culture medium DMEM and after 20 days, was performed quantitative analysis of the accession of first and second mesenchymal cells in cell culture bottles. RESULTS: The fat total cells (CTF) were 1.62 x10(6) cells/mL and presented 98% of viability. These cells were taken for cultivation and after 20 days were counted 2.88 x10(6) cells/mL MSC. The same was done and after 20 days we quantified 4.28 x10(6) cells/mL MSC. CONCLUSION: The lipectomy of adipose panicule is a very satisfactory method to extract stem cells from fat, quantitatively and qualitatively.

scholarly journals Volatiles from Steam-distilled Leaves of Some Plant Species from Madagascar and New Zealand and Evaluation of Their Biological Activity

2010 ◽  
Vol 5 (11) ◽  
pp. 1934578X1000501 ◽  
Author(s):  
Rosaria Costa ◽  
Francesco Pizzimenti ◽  
Francesca Marotta ◽  
P. Dugo ◽  
Luca Santi ◽  
...  
Keyword(s):  
Biological Activity ◽  
New Zealand ◽  
Quantitative Analysis ◽  
Plant Species ◽  
Cinnamomum Camphora ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Aerial Parts ◽  
Leptospermum Scoparium

Steam-distilled aerial parts of Ravensara Aromatica and Cinnamomum camphora from Madagascar and Leptospermum scoparium from New Zealand have been subjected to qualitative and quantitative analysis by means of GC techniques. This allowed the elucidation of conflicting data present in the available literature for these species. Also, the biological activity in vitro was evaluated by measuring MICs and GIZs.

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