scholarly journals Identification of geneticaly modified soybean seeds resistant to glyphosate

2004 ◽  
Vol 61 (3) ◽  
pp. 336-341 ◽  
Author(s):  
Maria Ângela André Tillmann ◽  
Shirlie West
Keyword(s):  
Genetically Modified ◽  
Soybean Seeds ◽  
Germination Test ◽  
Hypocotyl Length ◽  
Genetically Modified Soybean ◽  
Paper Towel ◽  
Soybean Genotypes ◽  
Physiological Quality ◽  
Resistance Trait ◽  
Moist Paper Towel

Advances in genetic engineering permit the modification of plants to be tolerant to certain herbicides that are usually not selective. For practical and commercial purposes, it is important to be able to detect the presence or absence of these traits in genotypes. The objective of this research was to develop a procedure for identifying genetically modified soybean (Glycine max L. Merr.) with resistance to the herbicide glyphosate. Two studies were conducted based on germination test. In the first study, soybean seeds were pre-imbibed in paper towel with the herbicide solutions, then transferred to moist paper towel for the germination test. In the second study, seeds were placed directly in herbicide solutions in plastic cups and tested for germination using the paper towel method. Eight soybean genotypes were compared: four Roundup Ready, that contained the gene resistant to the herbicide (G99-G725, Prichard RR, G99-G6682, and H7242 RR) and four non-transgenic parental cultivars (Boggs, Haskell, Benning, and Prichard). In the first study, the seeds were imbibed for 16 hours at 25°C in herbicide concentrations between 0.0 and 1.5% of the glyphosate active ingredient. In the second, seeds were subjected to concentrations between 0.0 and 0.48%, for one hour, at 30°C. The evaluation parameters were: germination, hypocotyl length, root length and total length of the seedlings. Both methods are efficient in identifying glyphosate-resistant soybean genotypes. It is possible to identify the genetically modified soybean genotypes after three days, by imbibing the seed in 0.12% herbicide solution, and after six days if the substrate is pre-imbibed in a 0.6% herbicide solution. The resistance trait was identified in all cultivars, independent of the initial physiological quality of the seed.

scholarly journals Alternative methods for detecting soybean seeds genetically modified for resistance to herbicide glyphosate

2013 ◽  
Vol 35 (3) ◽  
pp. 381-386
Author(s):  
Lilian Faria de Melo ◽  
Marcelo Fagioli ◽  
Marco Eustáquio de Sá
Keyword(s):  
Genetically Modified ◽  
Alternative Methods ◽  
Soybean Seeds ◽  
Transgenic Soybean ◽  
Genetically Modified Soybean ◽  
Soybean Cultivars ◽  
Seed Analysis ◽  
Glyphosate Herbicide ◽  
Herbicide Glyphosate

The objective of this study was to verify application of two methodologies: substrate moistened with herbicide solution (SM) and immersion of seeds in herbicide solution (IH) for detecting soybean seeds genetically modified. For this, non-transgenic and transgenic soybean seeds, harvested in the 2008/2009 crop seasons were used. The treatments with substrate moistened were: SM1) 0.03% herbicide solution, at 25 ºC, with evaluation in the sixth day (hs -0.03% -25 ºC, 6th d); SM2) HS -0.03% -35 ºC, 5th d; SM3) HS -0.03% - 40 ºC, 5th d; and SM4) hs -0.06% -5 ºC, 5th d. In the methodology of immersion of seeds the following treatments were performed: IH1) seed immersion in a 0.6% herbicide solution, at 25 ºC, for 1 h, (si -0.06% -25 ºC, 1 h; IH2) si -0.06% - 35 ºC, 30 min.; IH3) si -0.06% -40 ºC, 30 min.; IH4) si -0.12% -35 ºC, 30 min.; and IH5) si -0.12% -40 ºC, 30 min. Bioassays allow detecting soybean seeds tolerant to glyphosate herbicide within five days. The seeds of non-genetically modified and genetically modified soybean cultivars may be easily distinguished through the treatments SM2 and SM4 of the moistened substrate methodology; and treatments IH3, IH4, and IH5 of seed immersion methodology. Both methodologies are easily feasible, practical, and applicable in seed analysis laboratories, once do not require special equipments.

scholarly journals Spray volumes in the industrial treatment on the physiological quality of soybean seeds with different levels of vigor

2017 ◽  
Vol 39 (2) ◽  
pp. 174-181 ◽  
Author(s):  
Cristian Rafael Brzezinski ◽  
Julia Abati ◽  
Fernando Augusto Henning ◽  
Ademir Assis Henning ◽  
José de Barros França Neto ◽  
...  
Keyword(s):  
Experimental Design ◽  
Bradyrhizobium Japonicum ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Seed Vigor ◽  
Soybean Seeds ◽  
Germination Test ◽  
Physiological Quality ◽  
Different Levels

Abstract: The aim of this work was to evaluate the effect of different spray volumes, via industrial treatment, on the physiological quality of soybean seeds with different levels of vigor. The experimental design was completely randomized in a 2x5 factor scheme, with four replications. Factors consisted in two levels of seed vigor (high and low) and five spray volumes (0, 600, 1200, 1800 and 2400 mL. 100 kg-1). Products used to obtain the volumes were: fungicide (carbendazin + thiram); insecticide (imidacloprid + thiodicarb); nematicide (abamectin); micronutrients (cobalt, molybdenum and zinc); polymer (peridiam); biostimulants (kinetin + gibberellic acid) and inoculum (Bradyrhizobium japonicum). The used cultivars were BRS 360 RR and BRS 1010 IPRO. The physiological seed quality was determined by the following evaluations: germination, first count of the germination test, seedling emergence in sand, emergency speed index, total length of shoot and root of the seedlings. In the different spray volumes, high vigor soybean seeds have higher physiological quality than low vigor seeds. The increased spray volumes in seed treatments reduce the physiological quality of low vigor soybean seeds.

scholarly journals Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil

2014 ◽  
Vol 36 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Olívio F. Galão ◽  
Mercedes C. Carrão-Panizzi ◽  
José Marcos G. Mandarino ◽  
Rodrigo Santos Leite ◽  
Thiago Claus ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Trypsin Inhibitor ◽  
Genetically Modified ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor ◽  
Genetically Modified Soybean ◽  
South Brazil

scholarly journals Phytotoxicity in soybean seeds treated with phytosanitary products at different application times

2020 ◽  
Vol 42 ◽  
Author(s):  
Everson Reis Carvalho ◽  
Debora Kelli Rocha ◽  
Dayliane Bernardes de Andrade ◽  
Raquel Maria de Oliveira Pires ◽  
Amanda Carvalho Penido ◽  
...  
Keyword(s):  
Seed Treatment ◽  
Seed Quality ◽  
Soybean Seed ◽  
Field Performance ◽  
Soybean Seeds ◽  
Water Restriction ◽  
Hypocotyl Length ◽  
Initial Development ◽  
Physiological Quality ◽  
Phytosanitary Products

Abstract: Due to increased use of treated seeds, studies are necessary in relation to the effects of the treatments and their possible consequences on seed quality. The aim of this study was to evaluate phytotoxicity according to when phytosanitary products are applied on soybean seeds and their effect on seed physiological quality and field performance. A completely randomized experimental design was used in a 9 × 2 factorial arrangement involving 9 seed treatments (fungicides and insecticides) and two application times (60 days before sowing and at the time of sowing in the field). Moisture content was determined and tests were performed to ascertain physiological quality (germination and germination under water restriction). Root and hypocotyl length, seedling length, and vigor and uniformity indices were evaluated with the assistance of Vigor-S®. In the field, the following determinations were made: plant height at 30 and 60 days after emergence, first pod height, number of pods per plant, number of seeds per pod, and yield. Early seed treatment in the soybean crop can affect initial development of the plants, but it does not affect yield. The phytotoxicity caused by the soybean seed treatment was observed in the root length characteristic.

Anti-hypertensive activity of genetically modified soybean seeds accumulating novokinin

Peptides ◽  
2008 ◽  
Vol 29 (3) ◽  
pp. 331-337 ◽  
Author(s):  
Yuko Yamada ◽  
Keito Nishizawa ◽  
Megumi Yokoo ◽  
Hui Zhao ◽  
Kunihiko Onishi ◽  
...  
Keyword(s):  
Genetically Modified ◽  
Soybean Seeds ◽  
Genetically Modified Soybean ◽  
Hypertensive Activity

scholarly journals Efeito do tratamento químico com inseticida/fungicida e polímero na qualidade fisiológica da semente de soja - Effect of chemical treatment with insecticide / fungicide and polymer on the physiological quality of soybean seed

2017 ◽  
Vol 4 (1) ◽  
pp. 12 ◽  
Author(s):  
Evânia Alves ◽  
Elder Aguiar ◽  
Elder Aguiar ◽  
Célio Pereira ◽  
Célio Pereira ◽  
...  
Keyword(s):  
Glycine Max ◽  
Chemical Treatment ◽  
Seed Treatment ◽  
Soybean Seed ◽  
Soybean Seeds ◽  
Germination Test ◽  
M 10 ◽  
De Se ◽  
Physiological Quality

RESUMOA soja é uma das culturas mais cultivadas do planeta, sendo o Brasil, um de seus maiores produtores. É uma fonte barata e rica de óleo vegetal. O tratamento de sementes é amplamente utilizado para evitar perdas por patógenos e insetos na fase inicial do estabelecimento das plantas. Sendo uma das formas de se evitar a perda da qualidade. O objetivo deste trabalho foi avaliar o efeito do tratamento químico com inseticida/fungicida e polímero na qualidade final das sementes de soja. Foram utilizadas sementes do cultivar M-7110 Ipro, produzida na safra 2016/17 na região de Rio Verde, GO, com grau de umidade de 12%. Os tratamentos de sementes utilizados foram: Testemunha, Caixa vigor e praga (200 mL de Cruiser+ 100 mL de fipronil + 100 mL de Maxim XL); 100 mL + 100 mL de Standak + Maxim XL (Fipronil 250 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L) e 300 mL + 100 mL de Cropstar + Maxim XL (Imidacloprido 150 g/L e Tiodicarbe 450 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L). Foram utilizados o teste de germinação, emergência e a taxa de crescimento de plântulas. O tratamento Cropstar + Maxim XL se destacou como o melhor para o tratamento das sementes de soja quando comparado aos outros tratamentos utilizados neste trabalho. O uso de Standak + Maxim ML foi inferior aos demais para o tratamento de sementes de soja.Palavras-Chave: tratamento de sementes; Glycine max; vigor.ABSTRACTSoy is one of the most cultivated crops on the planet, being Brazil, one of its largest producers. It is a cheap, rich vegetable oil fountain. Seed treatment is widely used to avoid losses by pathogens and insects in the early stages of plant establishment. Being one of the ways to avoid the loss of quality. The purpose of this work was to evaluate the effect of chemical treatment with insecticide/fungicide and polymer in the final quality of soybean seeds. Seeds of cultivar M-7110 Ipro, produced in the harvest 2016/17 in the region of Rio Verde, GO, with a moisture content of 12% were used. The seeds treatments used were: Control, Caixa vigor e praga (200 mL de Cruiser+ 100 mL de fipronil + 100 mL de Maxim XL); 100 mL + 100 mL de Standak + Maxim XL (Fipronil 250 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L) e 300 mL + 100 mL de Cropstar + Maxim XL (Imidacloprido 150 g/L e Tiodicarbe 450 g/L + Metalaxil-M 10 g/L e Fludioxonil 25 g/L). The germination test, emergency and the growth rate of seedlings were tested. The treatment Cropstar + Maxim XL stood out as the best for treating soybean seeds when compared to other treatments used in this work. The use of Standak + Maxim ml was inferior to the other for the treatment of soybean seeds.Keywords: seed treatment; Glycine max; vigour

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