scholarly journals DETECTION OF GENETICALLY MODIFIED SOYBEAN SEEDS, (GLYCINE MAX, L. MERRIL), RESISTANT TO GLYPHOSATE, IN CONVENTIONAL SEED SAMPLES THROUGH BIOASSAY

2021 ◽  
Vol 10 (4) ◽  
pp. 1055-1055
Author(s):  
Luz Analia Almada Griffiths
Keyword(s):  
Glycine Max ◽  
Genetically Modified ◽  
Soybean Seeds ◽  
Genetically Modified Soybean ◽  
Glycine Max L

scholarly journals “Detección de semillas de soja genéticamente modificadas, (Glycine max, L. Merril), resistente al glifosato, en muestras de semillas convencionales a través del bioensayo” / “Detection of genetically modified soybean seeds, (Glycine max, L. Merril), resistant to glyphosate, in conventional seed samples through bioassay”

2021 ◽  
Vol 4 (3) ◽  
pp. 2887-2888
Author(s):  
Luz Almada ◽  
Liza Hepp
Keyword(s):  
Glycine Max ◽  
Genetically Modified ◽  
Soybean Seeds ◽  
Genetically Modified Soybean ◽  
Glycine Max L

A nivel mundial los organismos genéticamente modificados (GM) resistente a glifosato corresponde a 41 millones de hectáreas. La presencia de semillas de soja GM en lotes de semillas convencionales se ha convertido en un problema creciente para los comercios internacionales de la soja. Reconociendo la importancia de nuevos mercados  y los productos modificados genéticamente, la tecnología tendrá que asegurar la pureza de las semillas, la genética de los productos derivados de la biotecnología a través de pruebas confiables prácticos. En este contexto, los objetivos del experimento fueron comprobar la eficacia de las pruebas de germinación con herbicidas, en diferentes sustratos (Bioensayos) para la detección de las mezclas de cultivos modificados genéticamente en muestras de las semillas convencionales. Se seleccionaran, 2 lotes de semilla convencionales, BRS 282, V1 Vmax, y un lote de semillas genéticamente modificadas V2 MUNASQA. De las semillas genéticamente modificadas se establecieron diferentes niveles, para la mezcla con las semillas convencionales, N1 0, N2 2%, N3 4%, N4 6%. Se establecieron un total de cuatro tratamientos, y los datos obtenidos fueron analizados por análisis de regresión con coeficiente de determinación. Teniendo en cuenta el estado fisiológico de las plántulas según la clasificación en normales, anormales y muertas.Se puede concluir entonces que las semillas genéticamente modificadas con resistencia al glifosato mostraron diferencias en su desenvolvimiento al someterse a los test de germinación con determinada dosis de glifosato. Indicando que de entre los cuatro test utilizados el mejor fue el método de preimbibición.

scholarly journals The Nutritional Value and Safety of Genetically Unmodified Soybeans and Soybean Feed Products in the Nutrition of Farm Animals

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1105
Author(s):  
Małgorzata Świątkiewicz ◽  
Kamil Witaszek ◽  
Ewa Sosin ◽  
Krzysztof Pilarski ◽  
Beata Szymczyk ◽  
...  
Keyword(s):  
Glycine Max ◽  
Nutritional Value ◽  
Urease Activity ◽  
Basic Protein ◽  
Press Cake ◽  
Farm Animals ◽  
Soybean Seeds ◽  
Lower Content ◽  
Glycine Max L ◽  
Protein Feed

Post-extraction soybean (Glycine max (L.) Merr.) meal is widely used as a basic protein feed for farm animals, especially poultry and pigs. Products made from unmodified soybean seeds are an alternative to imported GMO soybean meal. The aim of the study was to develop feed products from popular European varieties of genetically unmodified soybeans, which can be produced on small and medium-sized farms, and to assess their nutritional value and safety to livestock. The research was conducted on the seeds of three soybean varieties and two types of feed products resulting from thermobaric treatment (extrudate) and oil pressing (soybean press cake). The mould and yeast contamination of domestic seeds was negligible. The thermobaric and pressing treatments lowered the content of fungi by 97%. The products were considered free from mycotoxins. In comparison with full-fat soybean seeds, the protein content in the products was up to 19% higher, and 92% of the total lysine remained available. The products had lower content of antinutritional ingredients (trypsin inhibitors) and the urease activity was reduced by 52–59% and 99%, respectively. The experiment showed that the European genetically unmodified soybean feed products were characterised by good quality, mycotoxicological purity and high nutritional value for farm animals.

scholarly journals Prioritization and Evaluation of Flooding Tolerance Genes in Soybean [Glycine max (L.) Merr.]

2021 ◽  
Vol 11 ◽  
Author(s):  
Mu-Chien Lai ◽  
Zheng-Yuan Lai ◽  
Li-Hsin Jhan ◽  
Ya-Syuan Lai ◽  
Chung-Feng Kao
Keyword(s):  
Glycine Max ◽  
Genome Wide Association Study ◽  
Meta Analysis ◽  
Soybean Seeds ◽  
Flooding Tolerance ◽  
Big Data Mining ◽  
Genome Wide ◽  
Glycine Max L ◽  
Mapping Gene ◽  
Selection Of

Soybean [Glycine max (L.) Merr.] is one of the most important legume crops abundant in edible protein and oil in the world. In recent years there has been increasingly more drastic weather caused by climate change, with flooding, drought, and unevenly distributed rainfall gradually increasing in terms of the frequency and intensity worldwide. Severe flooding has caused extensive losses to soybean production and there is an urgent need to breed strong soybean seeds with high flooding tolerance. The present study demonstrates bioinformatics big data mining and integration, meta-analysis, gene mapping, gene prioritization, and systems biology for identifying prioritized genes of flooding tolerance in soybean. A total of 83 flooding tolerance genes (FTgenes), according to the appropriate cut-off point, were prioritized from 36,705 test genes collected from multidimensional genomic features linking to soybean flooding tolerance. Several validation results using independent samples from SoyNet, genome-wide association study, SoyBase, GO database, and transcriptome databases all exhibited excellent agreement, suggesting these 83 FTgenes were significantly superior to others. These results provide valuable information and contribution to research on the varieties selection of soybean.

scholarly journals Identification of geneticaly modified soybean seeds resistant to glyphosate

2004 ◽  
Vol 61 (3) ◽  
pp. 336-341 ◽  
Author(s):  
Maria Ângela André Tillmann ◽  
Shirlie West
Keyword(s):  
Genetically Modified ◽  
Soybean Seeds ◽  
Germination Test ◽  
Hypocotyl Length ◽  
Genetically Modified Soybean ◽  
Paper Towel ◽  
Soybean Genotypes ◽  
Physiological Quality ◽  
Resistance Trait ◽  
Moist Paper Towel

Advances in genetic engineering permit the modification of plants to be tolerant to certain herbicides that are usually not selective. For practical and commercial purposes, it is important to be able to detect the presence or absence of these traits in genotypes. The objective of this research was to develop a procedure for identifying genetically modified soybean (Glycine max L. Merr.) with resistance to the herbicide glyphosate. Two studies were conducted based on germination test. In the first study, soybean seeds were pre-imbibed in paper towel with the herbicide solutions, then transferred to moist paper towel for the germination test. In the second study, seeds were placed directly in herbicide solutions in plastic cups and tested for germination using the paper towel method. Eight soybean genotypes were compared: four Roundup Ready, that contained the gene resistant to the herbicide (G99-G725, Prichard RR, G99-G6682, and H7242 RR) and four non-transgenic parental cultivars (Boggs, Haskell, Benning, and Prichard). In the first study, the seeds were imbibed for 16 hours at 25°C in herbicide concentrations between 0.0 and 1.5% of the glyphosate active ingredient. In the second, seeds were subjected to concentrations between 0.0 and 0.48%, for one hour, at 30°C. The evaluation parameters were: germination, hypocotyl length, root length and total length of the seedlings. Both methods are efficient in identifying glyphosate-resistant soybean genotypes. It is possible to identify the genetically modified soybean genotypes after three days, by imbibing the seed in 0.12% herbicide solution, and after six days if the substrate is pre-imbibed in a 0.6% herbicide solution. The resistance trait was identified in all cultivars, independent of the initial physiological quality of the seed.

Regional distribution in the fatty acids of triacylglycerols and phospholipids within soybean seeds (Glycine max L.)

2006 ◽  
Vol 108 (2) ◽  
pp. 149-158 ◽  
Author(s):  
Hiromi Yoshida ◽  
Sako Kanei ◽  
Yuka Tomiyama ◽  
Yoshiyuki Mizushina
Keyword(s):  
Fatty Acids ◽  
Glycine Max ◽  
Regional Distribution ◽  
Soybean Seeds ◽  
Glycine Max L

Metabolite profiling of isoflavones and anthocyanins in black soybean [Glycine max (L.) Merr.] seeds by HPLC-MS and geographical differentiation analysis in Southwest China

2017 ◽  
Vol 9 (5) ◽  
pp. 792-802 ◽  
Author(s):  
Hai-jun Wu ◽  
Jun-cai Deng ◽  
Cai-qiong Yang ◽  
Jing Zhang ◽  
Qing Zhang ◽  
...  
Keyword(s):  
Glycine Max ◽  
Statistical Analysis ◽  
Metabolite Profiling ◽  
Southwest China ◽  
Geographical Origin ◽  
Soybean Seeds ◽  
Geographical Differentiation ◽  
Multivariate Statistical ◽  
Black Soybean ◽  
Glycine Max L

Twelve isoflavones and eight anthocyanins were quantified in black soybean seeds by HPLC-MS analyses; the coupled OPLS-DA multivariate statistical analysis helped us to determine their geographical origin.

scholarly journals Alternative methods for detecting soybean seeds genetically modified for resistance to herbicide glyphosate

2013 ◽  
Vol 35 (3) ◽  
pp. 381-386
Author(s):  
Lilian Faria de Melo ◽  
Marcelo Fagioli ◽  
Marco Eustáquio de Sá
Keyword(s):  
Genetically Modified ◽  
Alternative Methods ◽  
Soybean Seeds ◽  
Transgenic Soybean ◽  
Genetically Modified Soybean ◽  
Soybean Cultivars ◽  
Seed Analysis ◽  
Glyphosate Herbicide ◽  
Herbicide Glyphosate

The objective of this study was to verify application of two methodologies: substrate moistened with herbicide solution (SM) and immersion of seeds in herbicide solution (IH) for detecting soybean seeds genetically modified. For this, non-transgenic and transgenic soybean seeds, harvested in the 2008/2009 crop seasons were used. The treatments with substrate moistened were: SM1) 0.03% herbicide solution, at 25 ºC, with evaluation in the sixth day (hs -0.03% -25 ºC, 6th d); SM2) HS -0.03% -35 ºC, 5th d; SM3) HS -0.03% - 40 ºC, 5th d; and SM4) hs -0.06% -5 ºC, 5th d. In the methodology of immersion of seeds the following treatments were performed: IH1) seed immersion in a 0.6% herbicide solution, at 25 ºC, for 1 h, (si -0.06% -25 ºC, 1 h; IH2) si -0.06% - 35 ºC, 30 min.; IH3) si -0.06% -40 ºC, 30 min.; IH4) si -0.12% -35 ºC, 30 min.; and IH5) si -0.12% -40 ºC, 30 min. Bioassays allow detecting soybean seeds tolerant to glyphosate herbicide within five days. The seeds of non-genetically modified and genetically modified soybean cultivars may be easily distinguished through the treatments SM2 and SM4 of the moistened substrate methodology; and treatments IH3, IH4, and IH5 of seed immersion methodology. Both methodologies are easily feasible, practical, and applicable in seed analysis laboratories, once do not require special equipments.

Inheritance of aconitase isozymes in soybean

Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 713-717 ◽  
Author(s):  
Joe-Yuan H. Doong ◽  
Yun-Tzu Kiang
Keyword(s):  
Glycine Max ◽  
Key Words ◽  
Gene Locus ◽  
Soybean Seeds ◽  
Genetic Studies ◽  
Intermediate Band ◽  
Linkage Analyses ◽  
Glycine Max L ◽  
Monomeric Structure

A six-band electrophoretic zymogram for aconitase was observed in mature soybean seeds. Among 750 Glycine max (L.) Merr. strains and cultivars examined, 10 zymogram types were identified. Genetic studies showed that the second band (Rf's of 0.35 or 0.42) and the fourth band (Rf's of 0.61, 0.63, or 0.69) in the zymograms were the products of two unlinked loci, Aco2 and Aco4, with two and three codominant alleles, respectively. Heterozygotes displayed both parental band sets without any intermediate band, indicating a monomeric structure for aconitase. The most common alleles at the two loci were Aco2-b (0.793) and Aco4-c (0.818). Linkage analyses revealed that both Aco2 and Aco4 loci are inherited independently of four other biochemical loci (Adh1, Am3, Ap, Dial). Key words: aconitase, isozymes, electrophoresis, gene locus, soybean, Glycine max.

scholarly journals Kunitz trypsin Inhibitor and phytic acid levels in conventional and genetically modified soybean seeds from Londrina and Ponta Grossa, South Brazil

2014 ◽  
Vol 36 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Olívio F. Galão ◽  
Mercedes C. Carrão-Panizzi ◽  
José Marcos G. Mandarino ◽  
Rodrigo Santos Leite ◽  
Thiago Claus ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Trypsin Inhibitor ◽  
Genetically Modified ◽  
Soybean Seeds ◽  
Kunitz Trypsin Inhibitor ◽  
Genetically Modified Soybean ◽  
South Brazil
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