Bovine gene polymorphisms related to fat deposition and meat tenderness

The micromolar calcium activated neutral protease (CAPN1) and calpastatin (CAST) have been widely regarded as genes related to muscle growth and meat tenderness. The objective of this study was to verify the association of SNPs of CAPN1 and CAST genes with carcass and tenderness traits and search the possible change patterns of SNPs in CAPN1 and CAST genes in six generations of broiler breeding process for growth rate, efficiency, and reproduction, during the third generation and the ninth generation, respectively. We found that, for CAPN1, genetic effects between SNPs (G3535A, C7198A) and meat tenderness were similar in different generations, while SNP3 (G7324A) was a novel polymorphism and had significant association with carcass and tenderness traits (P<0.05) in this study. Furthermore, there was significant association between SNP4 (G9950A) and carcass indexes instead of tenderness traits (P<0.05) which was consistent in the two generations. Moreover, although SNP6 (G37868A) of CAST had no relevance to carcass traits or tenderness traits in the third generation, it showed significant association with LW and CW in the ninth generation (P<0.05).

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Association of CAPN1 and CAST gene polymorphisms with meat tenderness in Bos taurus beef cattle from Argentina

Genetics and Molecular Biology ◽

10.1590/s1415-47572007000600006 ◽

2007 ◽

Vol 30 (4) ◽

pp. 1064-1069 ◽

Cited By ~ 30

Author(s):

Pablo Corva ◽

Liliana Soria ◽

Alejandro Schor ◽

Edgardo Villarreal ◽

Macarena Pérez Cenci ◽

...

Keyword(s):

Beef Cattle ◽

Gene Polymorphisms ◽

Bos Taurus ◽

Meat Tenderness ◽

Cast Gene

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The Differential Expressions of PPARγ and CAST mRNA in Muscle Tissues of Jinhua and Landrace Pigs

Indian Journal of Animal Research ◽

10.18805/ijar.b-1272 ◽

2020 ◽

Author(s):

Weixin Zhao ◽

Liping Guo ◽

Zhiguo Miao ◽

Jinzhou Zhang ◽

Shan Wang

Keyword(s):

Meat Quality ◽

Biceps Femoris ◽

Scientific Basis ◽

Fat Deposition ◽

Pork Quality ◽

Peroxisome Proliferator ◽

Meat Tenderness ◽

Peroxisome Proliferator Activated Receptor ◽

Biceps Femoris Muscle ◽

Muscle Tissues

Background: Fat deposition affected meat quality and the suitable amount of intramuscular fat (IMF) can significantly improve the taste of meat, palatability, juicy, tenderness and flavor, thereby enhancing the meat quality in animals. Although, calpastatin (CAST) and peroxisome proliferator-activated receptor-ã (PPARã) could regulate meat quality by altering meat tenderness or IMF content in animals, reports concerning the relationship between CAST, PPARã expression and pork quality is unclear.Methods: A total of twenty 28-day-old purebred weaned Jinhua and Landrace piglets (10 piglets per breed) were divided into two groups according to breed for the feeding trials lasting for 120 days. At the 148 days of age, 3 pigs of each breed were selected to slaughter. Total RNA was extracted from longissimus dorsi and biceps femoris muscle to investigate the differential expression of PPARã and CAST in muscle tissues of different breed pigs by RT-PCR methods, as well as their relationship with IMF and carcass lean content.Result: These results suggested that PPARã is an important effector for regulating fat deposition in pigs, which was correlated with IMF and carcass lean content of pigs. This data would provide a scientific basis for the regulation of pork quality.

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