In vitro evaluation of the whitening effect of mouth rinses containing hydrogen peroxide

Clinical Relevance Microleakage of a total-etch adhesive system was not influenced by the application of hydrogen peroxide; conversely, enamel dye penetration significantly increased for a self-etching primer.

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Effect of Mouth Rinse Treatments on Bleached Enamel Properties, Surface Morphology, and Tooth Color

Operative Dentistry ◽

10.2341/17-250-l ◽

2019 ◽

Vol 44 (2) ◽

pp. 178-187

Author(s):

WF Vieira-Junior ◽

LN Ferraz ◽

MCC Giorgi ◽

GMB Ambrosano ◽

FHB Aguiar ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Essential Oil ◽

Repeated Measures ◽

Linear Models ◽

Mouth Rinse ◽

Cross Sectional ◽

Tooth Color ◽

Color Changes ◽

Mouth Rinses

SUMMARY Objective: To evaluate, in vitro, the effect of mouth rinse exposure on bleached enamel. Methods: Enamel/dentin bovine blocks (4×4×2 mm) were bleached with 35% hydrogen peroxide (HP) and were submitted to immersion twice daily for 14 days with different rinses (n=10), including those involving: distilled water (C [control]), 225-ppm NaF (FM, Colgate Plax Classic), essential oil (EM, Listerine Tartar Control), 1.5% hydrogen peroxide (HPM, Colgate Plax Whitening), and 2% hydrogen peroxide, pyrophosphates, and 225-ppm NaF (HPM+P, Colgate® Luminous White). The specimens were stored in a remineralizing solution during all experiments. Analyses of color (ΔE, L*, a*, b*) and roughness (Ra) were performed at the baseline, after HP, and after exposure to the rinse. The cross-sectional microhardness (CSMH) and images by scanning electron microscopy (SEM) were assessed at the end. The data were subjected to analysis of variance (ANOVA) (ΔE), repeated measures ANOVA (Ra), and split-plot ANOVA (CSMH), followed by the Tukey test. The L*, a*, and b* values were analyzed by generalized linear models (α=0.05). Results: Color changes were not statistically different in the groups. Ra increased in all groups after bleaching; however, it was reestablished in C, FM, and HPM+F and increased in EM after 14 days of the rinse. EM and HPM reduced the CSMH values differing from C and promoted alterations on the enamel surface visualized by SEM. Conclusion: The mouth rinses did not affect the whitening efficacy or promote benefits on bleached enamel properties. Moreover, the 1.5% hydrogen peroxide– or essential oil–based mouth rinses affected the bleached enamel properties, promoting an alteration in morphologic surface and mineral loss in depth.

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In vitro tooth whitening effectiveness of whitening mouth rinses

Brazilian Journal of Oral Sciences ◽

10.20396/bjos.v19i0.8656779 ◽

2020 ◽

Vol 19 ◽

pp. e206779

Author(s):

Ana Paula Morona Rodrigues ◽

Mariele Cristina Klein ◽

Mauro Antonio Dall Agnol ◽

Sinval Adalberto Rodrigues-Junior

Keyword(s):

Hydrogen Peroxide ◽

Color Change ◽

Artificial Saliva ◽

Acrylic Resin ◽

Mouth Rinse ◽

Tooth Whitening ◽

Mouth Rinses ◽

Immersion Period ◽

Over Time

Regardless of the extensive availability of mouth rinses that claim to whiten teeth, evidence of achievement of such effect is still missing. Aim: Therefore, this study assessed in vitro the whitening effectiveness of whitening mouth rinses. Methods: Sixty intact bovine incisors were embedded in acrylic resin and had their buccal surface flattened and polished. Then, the specimens were randomly allocated to three conventional (Colgate Plax, Cepacol and Listerine Cool Mint) and three whitening mouth rinse groups (Colgate Luminous White, Cepacol Whitening and Listerine Whitening Extreme) (n=10). Following, the specimens were immersed twice a day in the mouth rinses for one minute for 28 days. In between each immersion period, the specimens remained in artificial saliva at 37oC. Color was measured at baseline, 7, 14, 21, and 28 days using a portable spectrophotometer (Easyshade, Vita Zahnfabrik, Germany) with a 6 mm of diameter probe. Color change was analyzed considering the parameters of ∆L*, ∆a*, ∆b* and, ultimately, ∆E*. The whitening efficacy of the mouth rinses was analyzed using the Whiteness Index for Dentistry (WID). Data of ∆s was analyzed using two-way ANOVA and Tukey’s test (α=0.05). Results: The type of mouth rinse affected significantly all the ∆ parameters (p<0.05). A non-whitening (conventional) mouth rinse produced the highest ΔE*, followed by the three whitening mouth rinses. The application time also affected ΔE* (p<0.05), with emphasis on the third week of treatment. Only the hydrogen peroxide-containing mouth rinse (Listerine Whitening Extreme) presented a whitening effect, with an increasing trend over time. Conclusion: Although the overall color change was not different when comparing conventional and whitening mouth rinses, the hydrogen peroxide-containing whitening mouth rinse produces an increasing whitening trend over time. Not every mouth rinse that claims to whiten teeth produces the desired effect.

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In Vitro Evaluation of the Risk of Inducing Bacterial Resistance to Disinfection Treatment with Photolysis of Hydrogen Peroxide

PLoS ONE ◽

10.1371/journal.pone.0081316 ◽

2013 ◽

Vol 8 (11) ◽

pp. e81316 ◽

Cited By ~ 19

Author(s):

Hiroyo Ikai ◽

Yu Odashima ◽

Taro Kanno ◽

Keisuke Nakamura ◽

Midori Shirato ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Bacterial Resistance ◽

In Vitro Evaluation

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Effect of exposure frequencies of pear juice on tooth whitening

Padjadjaran Journal of Dentistry ◽

10.24198/pjd.vol25no1.26795 ◽

2013 ◽

Vol 25 (1) ◽

Author(s):

Gobalakrishna Munusamy ◽

Endang Sukartini ◽

Ayu Trisna Hayati

Keyword(s):

Hydrogen Peroxide ◽

Research Result ◽

Control Group ◽

Tooth Whitening ◽

Whitening Effect ◽

Significant Difference ◽

Pear Juice ◽

The Difference ◽

Tooth Colour

Introduction: Tooth whitening (Bleaching) is a method to restore discoloured teeth to the original tooth colour with a chemical process. The materials used in the bleaching process are hydrogen peroxide, sodium perborates and carbamide peroxide. Pear fruit consists of hydrogen peroxide which is potentially an oxidizing compound that produces free radicals that react with unsaturated bonding teeth staining substances. This study aims to analyzed tooth whitening effect of exposure of pear juice with different frequencies. Methods: The type of research was true in vitro experiment where samples of 32 maxillary premolar teeth had cut the root to CEJ. The specimens divided into four groups. The first group was soaked in pear juice two times, the second group three times, a third group four times, and the fourth was the control group using only mineral water. Tooth colour was measure using a Spectrophometer, conducted before and after individual soaking. Results: The research result is tested with ANOVA and Newman- Keuls Range Test showed a significant difference of tooth whitening degree between the frequency exposure pear juice after 2 times soaking, 3 times soaking and 4 times soaking. Conclusion: There are difference degree of whitening from the difference frequency exposure of pear juice and Pear juice exposure of 4 times gives higher whitening effect compared to frequency of 3 times, 2 times and control group which is soaked in water.

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In vitro evaluation of a novel 6% hydrogen peroxide tooth whitening product

Journal of Dentistry ◽

10.1016/j.jdent.2003.10.007 ◽

2004 ◽

Vol 32 ◽

pp. 19-25 ◽

Cited By ~ 24

Author(s):

Andrew Joiner ◽

Gopal Thakker

Keyword(s):

Hydrogen Peroxide ◽

Tooth Whitening ◽

In Vitro Evaluation

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In vitro evaluation of experimental light activated gels for tooth bleaching

Photochemical & Photobiological Sciences ◽

10.1039/c8pp00223a ◽

2019 ◽

Vol 18 (5) ◽

pp. 1009-1019 ◽

Cited By ~ 4

Author(s):

Christoph Kurzmann ◽

Jeroen Verheyen ◽

Michael Coto ◽

Ramachandran Vasant Kumar ◽

Giorgio Divitini ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Metabolic Activity ◽

Tooth Bleaching ◽

In Vitro Evaluation

Gels containing TiO2/Ag or TiO2 particles are less cytotoxic than hydrogen peroxide-containing gels. TiO2/Ag gel exhibited a stronger reduction of metabolic activity and viability than TiO2 gel.

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Determination of the Whitening Effect of Toothpastes on Human Teeth

Odovtos - International Journal of Dental Sciences ◽

10.15517/ijds.2021.46376 ◽

2021 ◽

pp. 322-330

Author(s):

Numan Aydın ◽

Serpil Karaoğlanoğlu ◽

Elif Aybala Oktay ◽

Bilge Ersöz

Keyword(s):

Hydrogen Peroxide ◽

Activated Charcoal ◽

Single Point ◽

In Vitro Study ◽

Human Teeth ◽

Whitening Effect ◽

Significant Difference ◽

Electric Toothbrush ◽

Jaw Model

Objective: The desire of individuals to have whiter teeth increases the interest in tooth whitening products. Our aim was to in vitro study the whitening effect of hydrogen peroxide, blue covarine and active charcoal containing whitening toothpastes on human teeth. Materials and Methods: A total of 40 extracted human incisor teeth were used in the study. To measure the whitening effect of toothpastes, the teeth were divided into four subgroups and placed in the phantom tooth jaw model. Then, daily brushing was done with an electric toothbrush. The colors of the teeth were measured initially using the spectrophotometer (single point and bleached shade mode) and at the end of 7th, 14th and 28th days. Whitening effectiveness of toothpastes were studied according to CIEDE2000 formula (∆E00) and shade guide units (SGU). One-way analysis of variance (ANOVA) and Tukey test were used in the statistical analysis of the data. (p<0.05). Results: Activated charcoal containing toothpaste showed the greatest whitening effect at the end of 7th, 14th and 28th days (p<0.05). There was no statistically significant difference between the whitening effects of hydrogen peroxide and conventional toothpaste (p>0.05). Blue covarine containing toothpaste had statistically the lowest whitening effect (p<0.05). Conclusions: All toothpastes showed a whitening effect on the teeth after 7 days of use. Activated charcoal containing toothpaste showed more whitening effect after 28 days of use than hydrogen peroxide, blue covarine and traditional toothpaste.

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