scholarly journals Integrative taxonomy: combining molecular and morphological characteristics to identify Lymnaea (Galba) cubensis, intermediate host of Fasciola hepatica

Author(s):  
Ana Paula Pereira Neves Ferreira ◽  
Andréia Luiza Oliveira Costa ◽  
Raphael Meira Becattini ◽  
Mônica Alves Neves Diniz Ferreira ◽  
Hugo Pinto Rezende da Paixão ◽  
...  

Abstract Despite the epidemiological importance of the Lymnaeidae family regarding transmission of Fasciola hepatica, knowledge about the diversity and distribution of these molluscs and the role of each species in the expansion of fasciolosis remains sparse. Classical morphological (n=10) identification was performed in lymneids from Lagoa Santa, a municipality in the state of Minas Gerais, Brazil, along with molecular and phylogenetic analysis (n=05) based on the partial nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I gene (COI mtDNA) and ribosomal internal transcribed spacer II (ITS-2 rDNA). The shell morphology made it possible to distinguish the lymneids of Lagoa Santa from Pseudosuccinea columella. Differences found in the penile complex and prostate shape allowed this species to be distinguished from Galba truncatula. However, the homogeneity of reproductive tract characteristics among Lymnaea (Galba) cubensis, L. viator and L. neotropica confirmed that these characteristics show low taxonomic reliability for identifying cryptic species. Genetic divergence analysis for the COI mtDNA gene and ITS-2 region of rDNA revealed greater similarity to Lymnaea (Galba) cubensis. Thus, correct species differentiation is important for monitoring the epidemiological risk of fasciolosis in the state of Minas Gerais, where cases of the disease have increased over recent years.

2016 ◽  
Vol 25 (3) ◽  
pp. 364-367 ◽  
Author(s):  
Ruth Massote Dracz ◽  
Vinicius Marques Antunes Ribeiro ◽  
Cintia Aparecida de Jesus Pereira ◽  
Walter dos Santos Lima

Abstract Fasciola hepatica is a parasite that affects the hepatic ducts of several species of domestic and wild vertebrates, causing huge economic losses to livestock rearing worldwide. Reports on occurrences of F. hepatica in capybaras are an important epidemiological aspect of this disease, since these rodents can be a source of contamination for other animals and humans. In the present study, conducted in a rural area of the municipality of Confins, Minas Gerais, fresh feces from capybaras were collected from the ground near a lagoon at the edge of the Ribeirão da Mata river. These were examined using the technique of four metal sieves. F. hepatica eggs were recovered. This trematode species was confirmed by observing morphological characteristics and measuring the eggs recovered from the capybara feces, and through experimental infection of Lymnaea columella (Say, 1817) by miracidia from these eggs and subsequent infection of C57/BL06 mice with metacercariae originating from these infected mollusks. The data suggest the occurrence of natural cycle of F. hepatica in this region and provide a warning that expansion of the geographical distribution of this parasite by means of this rodent is possible. It is therefore important to adopting measures for epidemiological control of this helminthiasis.


2003 ◽  
Vol 77 (1) ◽  
pp. 7-10 ◽  
Author(s):  
L.H.L. Coelho ◽  
W.S. Lima

AbstractStudies on the population dynamics and natural infection of Lymnaea columella by Fasciola hepatica were carried out from September 1999 to December 2000 in a low-lying area near Itajubá in the Brazilian State of Minas Gerais. A total of 626 snails were collected monthly at nine different sites, counted, and dissected to search for larvae of F. hepatica. The highest populations of L. columella were reached in October of 1999 and August of 2000, and the highest natural infection rates of snails by F. hepatica were reached in September 1999 (5.2%) and July 2000 (3.9%). The removal by farmers of aquatic plants from the drainage furrows caused a drastic reduction in this snail population.


Author(s):  
Laís de Sousa Abreu Soares ◽  
Filipe Mendes Dalboni ◽  
Evandro Camargos Teixeira
Keyword(s):  

2016 ◽  
Vol 210 (1-2) ◽  
pp. 50-54 ◽  
Author(s):  
Diego H. Macedo ◽  
Armando Menezes-Neto ◽  
Jeronimo M. Rugani ◽  
Ana C. Rocha ◽  
Soraia O. Silva ◽  
...  

1992 ◽  
Vol 37 (sup2) ◽  
pp. 13-13
Author(s):  
Alexandre Soares Leal ◽  
Allan Sostenis Hanke
Keyword(s):  

2011 ◽  
Vol 7 (1) ◽  
pp. 68 ◽  
Author(s):  
Alessandro S Guimarães ◽  
Filipe B Carmo ◽  
Marcos B Heinemann ◽  
Ricardo WD Portela ◽  
Roberto Meyer ◽  
...  

1999 ◽  
Vol 9 (1) ◽  
pp. 147-154 ◽  
Author(s):  
Vytautas Kesminas ◽  
Tomas Virbickas ◽  
Saulius Stakėnas

Oryx ◽  
2014 ◽  
Vol 49 (1) ◽  
pp. 60-63 ◽  
Author(s):  
Guilherme Braga Ferreira ◽  
Marcelo Juliano Rabelo Oliveira ◽  
Rogério Cunha de Paula ◽  
Flávio Henrique Guimarães Rodrigues ◽  
Érica Daniele Cunha Carmo

AbstractThe bush dog Speothos venaticus, a rare Near Threatened South American canid that lives in packs, was thought to be extinct in Minas Gerais state, south-eastern Brazil, until recently. Here, we report four recent records of the species in Minas Gerais, the first in the state since the description of the species in 1842. All records are from the Cerrado ecosystem in the north and north-west of the state; two are from animals found dead, one from footprints and another from a camera trap. Three of the records were inside or close (< 10 km) to strict protected areas, in a region recognized as the Protected Areas Mosaic Sertão Veredas–Peruaçu, where we expect any new records of the bush dog to be found. We discuss the low probability of detecting the bush dog and the main regional threats to the species, and emphasize the need to protect large and interconnected natural areas and keep them free of domestic dogs to avoid the extinction of the bush dog in Minas Gerais.


2021 ◽  
Vol 22 ◽  
Author(s):  
Henrique Inhauser Riceti Magalhães ◽  
Ana Caroline Romão da Silva ◽  
Fabiano Braz Romão ◽  
Nadia Grandi Bombonato ◽  
Guilherme Nascimento Cunha

Abstract Among the diseases which can afflict the nasal cavities of small ruminants, oestrosis stands out. In Brazil, more specifically in its South-East region, the reports are limited only to the State of São Paulo and to the municipality of Araxá, Minas Gerais. Therefore, it has been sought to assess the parasitic prevalence of Oestrus ovis in sheep farmed in the municipality of Ituiutaba, Minas Gerais-Brazil, while correlating the larval size and stage, and its anatomical localization. Eighty-eight hemiheads of healthy Santa Inês/Dorper crossbreds Ovis aries have been used at random. The larvae in view were then collected and fixated to be quantified and analyzed in regard of size and stage of development. It is concluded that the oestrosis is an existing problem in the municipality of Ituiutaba, this being the first complete study on the prevalence of this parasite in the State of Minas Gerais. By anatomical distribution, only the differences of total larval averages between the frontal sinus and the ventral nasal meatus, the common nasal meatus and the nasopharynx have been significant. In size, the significant difference has been there only upon comparison between the size and the larval stage, information that is crucial for a better understanding of the cyclic progression, of the clinical symptomatology, and animal prophylaxis.


Sign in / Sign up

Export Citation Format

Share Document