scholarly journals Carotene and provitamin A content of vegetables sold in Viçosa, MG, Brazil, during spring and winter

2009 ◽  
Vol 45 (3) ◽  
pp. 527-537 ◽  
Author(s):  
Pollyanna Costa Cardoso ◽  
Ceres Mattos Della Lucia ◽  
Paulo César Stringheta ◽  
José Benício Paes Chaves ◽  
Helena Maria Pinheiro-Sant'Ana
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Leafy Vegetables ◽  
Provitamin A ◽  
Carotene Content ◽  
A Value ◽  
Β Carotene

This study investigated the α- and β-carotene content and provitamin A value of four leafy vegetables sold at local and street markets in Viçosa, MG, Brazil, in the spring and winter of 2002. Carotenoids were analyzed by high-performance liquid chromatography. α-Carotene was detected in all samples sold during spring, but was only present in a few samples of smooth and curly lettuce and kale in winter. β-Carotene was found in marked quantities in all leafy vegetables analyzed. Duncan's test (α = 5%) showed significantly higher α-carotene content in curly lettuce and vitamin A value in large-leaved watercress in the spring. Mean β-carotene content and vitamin A value were 7544, 8751, 2584, 2792, 8193, and 5338 μg/100 g and 666, 760, 227, 238, 698, and 460 μg RAE/100 g in large-leaved and hydroponic watercress, smooth and curly lettuce, kale and spinach, respectively. All leafy vegetables analyzed represent important sources of provitamin A and supply an important part of the daily requirements of children and adults.

Quantification of Provitamin A Compounds in ChInese Vegetables by High-Performance Liquid Chromatography

1993 ◽  
Vol 56 (1) ◽  
pp. 51-54 ◽  
Author(s):  
B. H. CHEN ◽  
J. R. CHUANG ◽  
J. H. LIN ◽  
C. P. CHIU
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Provitamin A ◽  
Photodiode Array Detection ◽  
Carotene Content ◽  
Green Pepper ◽  
Photodiode Array ◽  
Mustard Green ◽  
Β Carotene

The provitamin A contents of 16 vegetables grown in Taiwan were analyzed by high-performance liquid chromatography with photodiode-array detection. The amounts of the major provitamin A compounds, (β-cryptoxanthin, α-carotene, and β-carotene ranged from 0–6.8, 0–27.7, and 0.6–104.9 (μg/g, respectively. The highest β-carotene content was found in basil, followed by onion fragrant, kale, carrot, spinach, water convolvulus, mustard, green onion, garland chrysanthemum, sweet potato, green pepper, yellow com, mustard stem, lettuce, cabbage, and celeriac. Carrot was the only vegetable found to contain α-carotene while com was the vegetable to contain (β-cryptoxanthin.

scholarly journals Carrots and Dietary Vitamin A Adequacy

1992 ◽  
Vol 14 (2) ◽  
pp. 1-5 ◽  
Author(s):  
Lilian Portocarrero ◽  
Julieta Quan De Serrano ◽  
Louise Canfield ◽  
Thomas Tarara ◽  
Noel W. Solomons
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Latin American ◽  
High Performance ◽  
Dietary Vitamin ◽  
Carotene Content ◽  
Data Set ◽  
Diet Analyses ◽  
Β Carotene

Carrots are a leading source e of vitamin A in the Guatemalan diet. Analyses of carrots using high performance liquid chromatography (HPLC’) indicated that carrots have more than twice the amount of β carotene reported in the Latin American food compositio n tables. The consequence for the recalculation of daily vitamin A intake in a data set of pregnant pert-urban women is a 12% reduction in the number of women consuming <750 RE and a 19% reduction in the number with an intake of <600 RE. To the extent that earlier anayses underestimated the β carotene content of’ carrots, the estimates of vitamin A intake in Latin America have been variably and falsely low, depending on the contribution of carrots to the individuals reported diet.

Composition of butters on sale in Britain

1984 ◽  
Vol 51 (4) ◽  
pp. 637-641 ◽  
Author(s):  
David H. Buss ◽  
Patricia A. Jackson ◽  
David Scuffam
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
New Zealand ◽  
The Netherlands ◽  
Vitamin A ◽  
High Performance ◽  
Country Of Origin ◽  
Β Carotene

SummaryHigh-performance liquid chromatography was used to determine the amounts of all-trans retinol, 13-cis retinol and β-carotene in butters on sale in Britain. Samples originating in England and Ireland, New Zealand, Denmark and The Netherlands, and in France were analysed and each type of butter was measured in each season of the year. Fat, water, N and Na contents were also determined. The influence of season and country of origin on vitamin A content and on the level of salting are discussed.

Total Carotenoids Content and Assessment of Carotene Profile by High- Performance Liquid Chromatography in Selected Vegetables of Bangladesh with Special Reference to Some Unconventional Varieties

2020 ◽  
Vol 7 (1) ◽  
pp. 24-31
Author(s):  
Samia Sams ◽  
Mohammad Khairul Alam ◽  
Monira Ahsan ◽  
Sheikh Nazrul Islam
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Health Benefit ◽  
Food Composition ◽  
Leafy Vegetables ◽  
Immune Functions ◽  
Total Carotenoids

Background: Carotenoids are natural plant pigments and precursors of vitamin A, which provide a health benefit, protect against chronic degenerative diseases, and contribute to immune functions. Utilization and identification of foods with a high content of carotenoids received greater attention nowadays. Objective: The present study was aimed at evaluating total carotenoids content (TCC) and carotene composition of unconventional leafy vegetables growing in Bangladesh. Materials and Methods: The samples were collected from different locations of Bangladesh and mixed together to ensure sample representativeness. Acetone–petroleum ether extraction followed by spectrophotometric measurement was utilized for quantification of TCC. High-performance liquid chromatography (HPLC- DAD) was used to separate and quantify carotene components. Results and Discussion: The TCC values ranged from 129.38 μg/100 g edible portion (EP) in Helencha to 12803.31 μg/100 g EP in Roktodrone. The study findings also revealed that the selected plant samples exhibited high content of β-carotene ranging from 120.99 μg/100 g EP in Parul to 11301.16 μg/100 g EP in Roktodrone. It was also observed that most of the vegetables analyzed did not have α-carotene, lycopene, β-cryptoxanthin or it was in such a small quantity that could not be detected with HPLC. Conclusion: It can be suggested that regular intake of these vegetables may provide pro-vitamin A. In order to address the vitamin A deficiency and to maintain biodiversity, the analysis of carotenoids composition of these unconventional plant foods is to be initiated and make it available to the mass population. It would also fill up the data gap in the existing food composition table of Bangladesh.

Potential sources of bioactive compounds and antioxidants from 7 Brazilian native fruits

2020 ◽  
Vol 16 ◽  
Author(s):  
Fernanda Camboim Rockett ◽  
Helena de Oliveira Schmidt ◽  
Carlos Henrique Pagno ◽  
Jucelaine Possa ◽  
Renato Queiroz Assis ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
High Performance ◽  
Vitamin B3 ◽  
Trolox Equivalent ◽  
Equivalent Complex ◽  
Β Carotene

Background: Brazil is considered a country with the highest biological biodiversity in the world and exploring this underutilized food source could diversify food production and reduce nutritional problems. Objective: This study aimed to determine the antioxidant capacity and the bioactive compounds of seven native fruits from Brazil. Method: The samples were obtained from different cities of Brazil after confirmation of the botanical species. Carotenoids and anthocyanins were exhaustively extracted and injected into high-performance liquid chromatography. The antioxidant capacity was determined by the capture of the free radical ABTS. Vitamin A activity was calculated based on retinol activity equivalent. Complex B and C vitamins were determined by high-performance liquid chromatography. Result: The main carotenoid for all species was β-carotene, and Jaracatiá showed the highest amount of β-cryptoxanthin, zeaxanthin, α- and β-carotene. Açaí Juçara and the Butiás stood out among fruits analyzed with the highest antioxidant capacity (trolox equivalent). Concerning the anthocyanins, in the Açaí Juçara were identified the compounds Cyanidin 3-glucoside and Cyanidin 3-rutinoside. The values of all the compounds vary according to the harvest location. Jaracatiá is distinguished by the high vitamin A, C, B2, and B6 content, as well the Butiás (Praia and Serra), for B1, B2, B5 and B7. Vitamin B3 was only detected in Fisális fruits and Açaí showed higher content of B5. Conclusion: Fruits included in this study had considerable amounts of bioactive compounds, vitamins and antioxidant capacity showing that they can contribute to the improvement of the population diet.

Quantification of Vitamin A in Fortified Rapeseed, Groundnut and Soya Oils Using a Simple Portable Device: Comparison to High Performance Liquid Chromatography

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Developed Countries ◽  
Portable Device ◽  
Least Developed Countries ◽  
Control Food ◽  
Assay Precision

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.

Vitamin A Value of Plant Food Provitamin A - Evaluated by the Stable Isotope Technologies

2014 ◽  
Vol 84 (Supplement 1) ◽  
pp. 25-29 ◽  
Author(s):  
Guangwen Tang
Keyword(s):  
Stable Isotope ◽  
Vitamin A ◽  
Provitamin A ◽  
Animal Origin ◽  
Plant Food ◽  
Plant Foods ◽  
A Value ◽  
Provitamin A Carotenoids ◽  
Β Carotene

Humans need vitamin A and obtain essential vitamin A by conversion of plant foods rich in provitamin A and/or absorption of preformed vitamin A from foods of animal origin. The determination of the vitamin A value of plant foods rich in provitamin A is important but has challenges. The aim of this paper is to review the progress over last 80 years following the discovery on the conversion of β-carotene to vitamin A and the various techniques including stable isotope technologies that have been developed to determine vitamin A values of plant provitamin A (mainly β-carotene). These include applications from using radioactive β-carotene and vitamin A, depletion-repletion with vitamin A and β-carotene, and measuring postprandial chylomicron fractions after feeding a β-carotene rich diet, to using stable isotopes as tracers to follow the absorption and conversion of plant food provitamin A carotenoids (mainly β-carotene) in humans. These approaches have greatly promoted our understanding of the absorption and conversion of β-carotene to vitamin A. Stable isotope labeled plant foods are useful for determining the overall bioavailability of provitamin A carotenoids from specific foods. Locally obtained plant foods can provide vitamin A and prevent deficiency of vitamin A, a remaining worldwide concern.

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