Potential sources of bioactive compounds and antioxidants from 7 Brazilian native fruits

2020 ◽  
Vol 16 ◽  
Author(s):  
Fernanda Camboim Rockett ◽  
Helena de Oliveira Schmidt ◽  
Carlos Henrique Pagno ◽  
Jucelaine Possa ◽  
Renato Queiroz Assis ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
High Performance ◽  
Vitamin B3 ◽  
Trolox Equivalent ◽  
Equivalent Complex ◽  
Β Carotene

Background: Brazil is considered a country with the highest biological biodiversity in the world and exploring this underutilized food source could diversify food production and reduce nutritional problems. Objective: This study aimed to determine the antioxidant capacity and the bioactive compounds of seven native fruits from Brazil. Method: The samples were obtained from different cities of Brazil after confirmation of the botanical species. Carotenoids and anthocyanins were exhaustively extracted and injected into high-performance liquid chromatography. The antioxidant capacity was determined by the capture of the free radical ABTS. Vitamin A activity was calculated based on retinol activity equivalent. Complex B and C vitamins were determined by high-performance liquid chromatography. Result: The main carotenoid for all species was β-carotene, and Jaracatiá showed the highest amount of β-cryptoxanthin, zeaxanthin, α- and β-carotene. Açaí Juçara and the Butiás stood out among fruits analyzed with the highest antioxidant capacity (trolox equivalent). Concerning the anthocyanins, in the Açaí Juçara were identified the compounds Cyanidin 3-glucoside and Cyanidin 3-rutinoside. The values of all the compounds vary according to the harvest location. Jaracatiá is distinguished by the high vitamin A, C, B2, and B6 content, as well the Butiás (Praia and Serra), for B1, B2, B5 and B7. Vitamin B3 was only detected in Fisális fruits and Açaí showed higher content of B5. Conclusion: Fruits included in this study had considerable amounts of bioactive compounds, vitamins and antioxidant capacity showing that they can contribute to the improvement of the population diet.

Composition of butters on sale in Britain

1984 ◽  
Vol 51 (4) ◽  
pp. 637-641 ◽  
Author(s):  
David H. Buss ◽  
Patricia A. Jackson ◽  
David Scuffam
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
New Zealand ◽  
The Netherlands ◽  
Vitamin A ◽  
High Performance ◽  
Country Of Origin ◽  
Β Carotene

SummaryHigh-performance liquid chromatography was used to determine the amounts of all-trans retinol, 13-cis retinol and β-carotene in butters on sale in Britain. Samples originating in England and Ireland, New Zealand, Denmark and The Netherlands, and in France were analysed and each type of butter was measured in each season of the year. Fat, water, N and Na contents were also determined. The influence of season and country of origin on vitamin A content and on the level of salting are discussed.

scholarly journals Carrots and Dietary Vitamin A Adequacy

1992 ◽  
Vol 14 (2) ◽  
pp. 1-5 ◽  
Author(s):  
Lilian Portocarrero ◽  
Julieta Quan De Serrano ◽  
Louise Canfield ◽  
Thomas Tarara ◽  
Noel W. Solomons
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Latin American ◽  
High Performance ◽  
Dietary Vitamin ◽  
Carotene Content ◽  
Data Set ◽  
Diet Analyses ◽  
Β Carotene

Carrots are a leading source e of vitamin A in the Guatemalan diet. Analyses of carrots using high performance liquid chromatography (HPLC’) indicated that carrots have more than twice the amount of β carotene reported in the Latin American food compositio n tables. The consequence for the recalculation of daily vitamin A intake in a data set of pregnant pert-urban women is a 12% reduction in the number of women consuming <750 RE and a 19% reduction in the number with an intake of <600 RE. To the extent that earlier anayses underestimated the β carotene content of’ carrots, the estimates of vitamin A intake in Latin America have been variably and falsely low, depending on the contribution of carrots to the individuals reported diet.

scholarly journals Carotene and provitamin A content of vegetables sold in Viçosa, MG, Brazil, during spring and winter

2009 ◽  
Vol 45 (3) ◽  
pp. 527-537 ◽  
Author(s):  
Pollyanna Costa Cardoso ◽  
Ceres Mattos Della Lucia ◽  
Paulo César Stringheta ◽  
José Benício Paes Chaves ◽  
Helena Maria Pinheiro-Sant'Ana
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Leafy Vegetables ◽  
Provitamin A ◽  
Carotene Content ◽  
A Value ◽  
Β Carotene

This study investigated the α- and β-carotene content and provitamin A value of four leafy vegetables sold at local and street markets in Viçosa, MG, Brazil, in the spring and winter of 2002. Carotenoids were analyzed by high-performance liquid chromatography. α-Carotene was detected in all samples sold during spring, but was only present in a few samples of smooth and curly lettuce and kale in winter. β-Carotene was found in marked quantities in all leafy vegetables analyzed. Duncan's test (α = 5%) showed significantly higher α-carotene content in curly lettuce and vitamin A value in large-leaved watercress in the spring. Mean β-carotene content and vitamin A value were 7544, 8751, 2584, 2792, 8193, and 5338 μg/100 g and 666, 760, 227, 238, 698, and 460 μg RAE/100 g in large-leaved and hydroponic watercress, smooth and curly lettuce, kale and spinach, respectively. All leafy vegetables analyzed represent important sources of provitamin A and supply an important part of the daily requirements of children and adults.

Quantification of Vitamin A in Fortified Rapeseed, Groundnut and Soya Oils Using a Simple Portable Device: Comparison to High Performance Liquid Chromatography

2013 ◽  
Vol 83 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Cécile Renaud ◽  
Jacques Berger ◽  
Arnaud Laillou ◽  
Sylvie Avallone
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance ◽  
Vitamin A Deficiency ◽  
Developed Countries ◽  
Portable Device ◽  
Least Developed Countries ◽  
Control Food ◽  
Assay Precision

Vitamin A deficiency is still one of the major public health problems in least developed countries. Fortification of vegetable oils is a strategy implemented worldwide to prevent this deficiency. For a fortification program to be effective, regular monitoring is necessary to control food quality in the producing units. The reference methods for vitamin A quantification are expensive and time-consuming. A rapid method should be useful for regular assessment of vitamin A in the oil industry. A portable device was compared to high-performance liquid chromatography (HPLC) for three plant oils (rapeseed, groundnut, and soya). The device presented a good linearity from 3 to 30 mg retinol equivalents per kg (mg RE.kg- 1). Its limits of detection and quantification were 3 mg RE.kg- 1 for groundnut and rapeseed oils and 4 mg RE.kg- 1 for soya oil. The intra-assay precision ranged from 1.48 % to 3.98 %, considered satisfactory. Accuracy estimated by the root mean squares error ranged from 3.99 to 5.49 and revealed a lower precision than HPLC (0.4 to 2.25). Although it offers less precision than HPLC, the device estimates quickly the vitamin A content of the tested oils from 3 or 4 to 15 mg RE.kg- 1.

scholarly journals Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1371 ◽  
Author(s):  
Vanesa Nuñez-Gómez ◽  
Nieves Baenas ◽  
Inma Navarro-González ◽  
Javier García-Alonso ◽  
Diego A. Moreno ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Methyl Jasmonate ◽  
Bioactive Compounds ◽  
High Performance ◽  
Total Phenolic ◽  
Diode Array ◽  
Total Carotenoids ◽  
Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kela

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
LILY RESTUSARI ◽  
SRI WIDIA NINGSIH ◽  
YULIANA ARSIL
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
High Performance

Tingginya tingkat konsumsi minyak goreng oleh Masyarakat Indonesia menjadikan minyak goreng digunakan sebagai food vehiclepada program fortifikasi vitamin A.Program tersebut dilakukan untuk mengatasi Kekurangan vitamin A (KVA). KVAsangat mempengaruhi kelangsungan hidup anak dan standar upaya penyelamatan proses kehamilan dan persalinan. Namun pemanasan yang terjadi selama proses penggorenganpada minyak dapatmerusak jumlah vitamin A yang difortifikasi. Tujuan penelitian ini untuk membandingkan nilai retensi vitamin A di dalam kentang yang digoreng dengan menggunakan minyak goreng curah dan minyak goreng kemasan fortifikasi dengan pengaruh suhu dan waktu penggorengan. Penelitian ini menggunakan metode eksperimental disain rancangan acak lengkap (RAL) dengan dua faktor, suhu (140dan 170oC) dan waktu penggorengan (10 dan 15 menit) tanpa pengulangan.Sampel penelitian yang digunakan adalah kentang beku, minyak goreng curah dan minyak goreng kemasan fortifikasi.Nilai retensi vitamin A kentang dan minyak goreng diukur dengan menggunakan metode High Performance Liquid Chromatography (HPLC).Analisis perbedaan dan interaksi variabel diolah dengan ANOVA dua arah. Berdasarkan luas area puncak vitamin A pada kromatogram sebelum dan setelah penggorengan, ditemukan kecenderungan penurunan persentase retensi vitamin A dari minyak goreng kemasan fortifikasi dibandingkan minyak goreng curah.Nilai retensi vitamin A pada kentang yang digoreng dengan minyak goreng curah adalah 217-251%,sedangkan retensivitamin A kentang yang digoreng dengan minyak goreng kemasan fortifikasi adalah 48-131%. Analisis ANOVA dua arah tanpa pengulangan untuk persen retensi vitamin A menunjukkan bahwa (p<0,05) variabel jenis minyak goreng berpengaruh secara nyata terhadap retensi vitamin A. Retensi vitamin A pada kentang goreng yang digoreng dengan minyak goreng curah jauh lebih tinggi dibanding minyak goreng kemasan fortifikasi

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