scholarly journals Studies on the Emergence of Rice Seedlings Seeded in Autumn and Winter : II. Some physiological properties of soaking seeds under low temperature

1969 ◽  
Vol 38 (1) ◽  
pp. 111-116
Author(s):  
Masami HIMEDA
Keyword(s):  
Low Temperature ◽  
Rice Seedlings ◽  
Physiological Properties ◽  
Autumn And Winter

scholarly journals Studies on the Low-temperature Damage in Rice Seedlings. Effect of low temperature on the growth and bleeding rates of the root.

2001 ◽  
Vol 70 (4) ◽  
pp. 561-567
Author(s):  
Zhijian YANG ◽  
Osamu SASAKI ◽  
Tomohide SHIMOTASHIRO ◽  
Akinori NAKAGAMA
Keyword(s):  
Low Temperature ◽  
Rice Seedlings ◽  
Temperature Damage

scholarly journals Transcriptomic, proteomic, and physiological comparative analyses of flooding mitigation of the damage induced by low-temperature stress in direct seeded early indica rice at the seedling stage

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Wenxia Wang ◽  
Jie Du ◽  
Liming Chen ◽  
Yongjun Zeng ◽  
Xueming Tan ◽  
...  
Keyword(s):  
Low Temperature ◽  
Temperature Stress ◽  
Molecular Mechanisms ◽  
Tricarboxylic Acid Cycle ◽  
Indica Rice ◽  
Low Temperature Stress ◽  
Seedling Stage ◽  
Differentially Expressed ◽  
Rice Seedlings ◽  
Direct Seeded

Abstract Background Low temperature (LT) often occurs at the seedling stage in the early rice-growing season, especially for direct seeded early-season indica rice, and using flooding irrigation can mitigate LT damage in rice seedlings. The molecular mechanism by which flooding mitigates the damage induced by LT stress has not been fully elucidated. Thus, LT stress at 8 °C, LT accompanied by flooding (LTF) and CK (control) treatments were established for 3 days to determine the transcriptomic, proteomic and physiological response in direct seeded rice seedlings at the seedling stage. Results LT damaged chloroplasts, and thylakoid lamellae, and increased osmiophilic bodies and starch grains compared to CK, but LTF alleviated the damage to chloroplast structure caused by LT. The physiological characteristics of treated plants showed that compared with LT, LTF significantly increased the contents of rubisco, chlorophyll, PEPCK, ATP and GA3 but significantly decreased soluble protein, MDA and ABA contents. 4D-label-free quantitative proteomic profiling showed that photosynthesis-responsive proteins, such as phytochrome, as well as chlorophyll and the tricarboxylic acid cycle were significantly downregulated in LT/CK and LTF/CK comparison groups. However, compared with LT, phytochrome, chlorophyllide oxygenase activity and the glucan branching enzyme in LTF were significantly upregulated in rice leaves. Transcriptomic and proteomic studies identified 72,818 transcripts and 5639 proteins, and 4983 genes that were identified at both the transcriptome and proteome levels. Differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were significantly enriched in glycine, serine and threonine metabolism, biosynthesis of secondary metabolites, glycolysis/gluconeogenesis and metabolic pathways. Conclusion Through transcriptomic, proteomic and physiological analyses, we determined that a variety of metabolic pathway changes were induced by LT and LTF. GO and KEGG enrichment analyses demonstrated that DEGs and DEPs were associated with photosynthesis pathways, antioxidant enzymes and energy metabolism pathway-related proteins. Our study provided new insights for efforts to reduce the damage to direct seeded rice caused by low-temperature stress and provided a breeding target for low temperature flooding-resistant cultivars. Further analysis of translational regulation and metabolites may help to elucidate the molecular mechanisms by which flooding mitigates low-temperature stress in direct seeded early indica rice at the seedling stage.

scholarly journals Biochar’s Leacheates Affect the Abscisic Acid Pathway in Rice Seedlings Under Low Temperature

2021 ◽  
Vol 12 ◽  
Author(s):  
Jun Yuan ◽  
Jun Meng ◽  
Xiao Liang ◽  
E Yang ◽  
Xu Yang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Molecular Docking ◽  
Low Temperature ◽  
Signaling Pathway ◽  
Organic Molecules ◽  
Cold Resistance ◽  
Water Soluble ◽  
Aba Signaling ◽  
Rice Seedlings ◽  
Endogenous Aba

Organic molecules of biochar’s leacheates are known to increase the cold resistance of rice seedlings. Yet, it remains unclear whether the organic molecules of biochar leacheates can interact with the abscisic acid (ABA) signaling pathway associated with low temperature. This study used experiments and bioinformatics (molecular docking) to determine which of the organic molecules of biochar’s leacheates could influence the ABA signaling pathway. Specifically, we investigated whether these molecules affected ABA, a plant hormone linked to cold resistance. The contents of endogenous ABA and its precursor carotenoids were determined under low-temperature stress (10°C) and treatment with different concentrations of biochar leacheates. With increased leacheate concentrations, the endogenous ABA and carotenoid contents also increased, as did the expression of ABA- and cold-related genes. When rice seedlings were instead treated with exogenous ABA, it also affected the above-measured indexes; hence, we surmised that certain water-soluble organic molecules of biochar could exert a similar effect as ABA. We first used gas chromatography/mass spectrometry (GC/MS) to identify the organic molecules in the biochar extract, and then we used molecular docking software Autodock to show how they interact. We found that the molecule (1R, 2R, 4S)-2-(6-chloropyridin-3-yl)-7-azabicyclo(2.2.1)heptane was simplified, as Cyah could dock with the ABA receptor protein OsPYL2 in rice, which shows Cyah in biochar is probably an analog of ABA, with a similar function. Based on these results, we conclude that organic molecules of biochar’s leacheates could enter into rice plants and interact with ABA-related proteins to affect the ABA signaling pathway, thereby improving the cold stress resistance of plants.

scholarly journals Low-Temperature Accumulation of Alcohol Dehydrogenase-1 mRNA and Protein Activity in Maize and Rice Seedlings

1991 ◽  
Vol 95 (3) ◽  
pp. 699-706 ◽  
Author(s):  
Peter J. Christie ◽  
Matthias Hahn ◽  
Virginia Walbot
Keyword(s):  
Low Temperature ◽  
Alcohol Dehydrogenase ◽  
Rice Seedlings ◽  
Protein Activity ◽  
Alcohol Dehydrogenase 1

scholarly journals Transcriptomic, proteomic, and physiological comparative analyses of flooding mitigating low temperature stress on direct-seeded early indica rice at seedling stage

2020 ◽  
Author(s):  
WENXIA WANG ◽  
Jie Du ◽  
Liming Chen ◽  
Yongjun Zeng ◽  
Xueming Tan ◽  
...  
Keyword(s):  
Low Temperature ◽  
Metabolic Regulation ◽  
Indica Rice ◽  
Physiological Activity ◽  
Growth Characteristic ◽  
Seedling Stage ◽  
Differentially Expressed ◽  
Rice Seedlings ◽  
Direct Seeded ◽  
Early Rice

Abstract Background Low temperature (LT) often occurs at seedling stage in early rice-growing season, especially for direct-seeded early-season indica rice seedlings, and adopting flooding irrigation could mitigate LT damage. However, it is not clear on the response mechanisms of a mitigating effect on LT stress at early rice seedling stage. Results In this study, LT stress with 10/6°C (day/night), LT accompanied by flooding (LTF) and CK (control) treatments were established for 3d to aim to determine the response mechanisms on physiological, transcriptomic, and proteomic of direct-seeded rice seedlings at seedling stage. The results showed that the chloroplasts was severely degraded, thylakoid lamellae were seriously damaged and osmiophilic body increased gradually in LT contrast to CK, but LTF could alleviate the damage of low temperature on chloroplast structure. Compared with LT, LTF significantly increased the contents of Rubisco, chlorophyll, PEPCK, ATP and GA3 of rice seedlings whereas significantly decreased soluble protein, MDA and ABA content, suggesting the higher photosynthetic traits, antioxidant ability and better growth characteristic, although it could also affect the physiological activity contrast to CK. The identified differentially expressed genes and differentially expressed proteins indicated that photosynthesis metabolism pathway, reactive oxygen species and metabolic regulation had significant differences between LT and LTF stress, which were the main reasons that reduced the LT damage of rice seedlings for LTF. Conclusions Our results could provide comprehensive interpretation of physiological characteristics, genes and proteins expression changes in low temperature and low temperature flooding.

scholarly journals Rooting Ability in Rice Seedlings under Low Temperature : I. Potentiality of re-rooting

1957 ◽  
Vol 26 (2) ◽  
pp. 154-155
Author(s):  
Reizo AIMI ◽  
Haruhiko NAKAYAMA
Keyword(s):  
Low Temperature ◽  
Rice Seedlings ◽  
Rooting Ability
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