scholarly journals Biochar’s Leacheates Affect the Abscisic Acid Pathway in Rice Seedlings Under Low Temperature

2021 ◽  
Vol 12 ◽  
Author(s):  
Jun Yuan ◽  
Jun Meng ◽  
Xiao Liang ◽  
E Yang ◽  
Xu Yang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Molecular Docking ◽  
Low Temperature ◽  
Signaling Pathway ◽  
Organic Molecules ◽  
Cold Resistance ◽  
Water Soluble ◽  
Aba Signaling ◽  
Rice Seedlings ◽  
Endogenous Aba

Organic molecules of biochar’s leacheates are known to increase the cold resistance of rice seedlings. Yet, it remains unclear whether the organic molecules of biochar leacheates can interact with the abscisic acid (ABA) signaling pathway associated with low temperature. This study used experiments and bioinformatics (molecular docking) to determine which of the organic molecules of biochar’s leacheates could influence the ABA signaling pathway. Specifically, we investigated whether these molecules affected ABA, a plant hormone linked to cold resistance. The contents of endogenous ABA and its precursor carotenoids were determined under low-temperature stress (10°C) and treatment with different concentrations of biochar leacheates. With increased leacheate concentrations, the endogenous ABA and carotenoid contents also increased, as did the expression of ABA- and cold-related genes. When rice seedlings were instead treated with exogenous ABA, it also affected the above-measured indexes; hence, we surmised that certain water-soluble organic molecules of biochar could exert a similar effect as ABA. We first used gas chromatography/mass spectrometry (GC/MS) to identify the organic molecules in the biochar extract, and then we used molecular docking software Autodock to show how they interact. We found that the molecule (1R, 2R, 4S)-2-(6-chloropyridin-3-yl)-7-azabicyclo(2.2.1)heptane was simplified, as Cyah could dock with the ABA receptor protein OsPYL2 in rice, which shows Cyah in biochar is probably an analog of ABA, with a similar function. Based on these results, we conclude that organic molecules of biochar’s leacheates could enter into rice plants and interact with ABA-related proteins to affect the ABA signaling pathway, thereby improving the cold stress resistance of plants.

scholarly journals Control of ABA Signaling and Crosstalk With Other Hormones by Selective Degradation of Pathway Components

2021 ◽  
Author(s):  
Agnieszka Sirko ◽  
Anna Wawrzyńska ◽  
Jerzy Brzywczy ◽  
Marzena Sieńko
Keyword(s):  
Abscisic Acid ◽  
Signaling Pathway ◽  
Plant Hormone ◽  
Aba Signaling ◽  
Degradation Mechanisms ◽  
E3 Ligases ◽  
Selective Autophagy ◽  
Selective Degradation ◽  
Different Parts

A rapid and appropriate genetic and metabolic acclimation, which is crucial for plants’ survival in a changing environment, is maintained due to the coordinated action of plant hormones and cellular degradation mechanisms influencing proteostasis. The plant hormone abscisic acid (ABA) rapidly accumulates in plants in response to environmental stress and plays a pivotal role in the reaction to various stimuli. Increasing evidence demonstrates a significant role of autophagy in controlling ABA signaling. This field has been extensively investigated and new discoveries are constantly being provided. We present updated information on the components of the ABA signaling pathway, particularly on transcription factors modified by different E3 ligases. Then, we focus on the role of selective autophagy in ABA pathway control and review novel evidence on the involvement of autophagy in different parts of the ABA signaling pathway that are important for crosstalk with other hormones, particularly cytokinins and brassinosteroids.

scholarly journals VlbZIP30 of grapevine functions in drought tolerance via the abscisic acid core signaling pathway

2017 ◽  
Author(s):  
Mingxing Tu ◽  
Xianhang Wang ◽  
Yanxun Zhu ◽  
Dejun Wang ◽  
Xuechuan Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Drought Tolerance ◽  
Signaling Pathway ◽  
Growth And Development ◽  
Transgenic Arabidopsis ◽  
Dehydration Stress ◽  
Aba Signaling ◽  
Group A ◽  
Cotyledon Greening

AbstractDrought stress limits the growth and development of grapevines, thereby reducing productivity, but the mechanisms by which grapevines respond to drought stress remain largely uncharacterized. Here, we characterized a group A bZIP gene from ‘Kyoho’ grapevine, VlbZIP30, which was shown to be induced by abscisic acid (ABA) and dehydration stress. Overexpression of VlbZIP30 in transgenic Arabidopsis enhanced dehydration tolerance during seed germination, and in the seedling and adult stages. Transcriptome analysis revealed that a major proportion of ABA- and/or drought-responsive genes are transcriptionally regulated by VlbZIP30 during ABA or mannitol treatment at the cotyledon greening stage. We identified an A. thaliana G-box motif (CACGTG) and a potential grapevine G-box motif (MCACGTGK) in the promoters of the 39 selected A. thaliana genes up-regulated in the transgenic plants and in the 35 grapevine homologs, respectively. Subsequently, using two grapevine-related databases, we found that 74% and 84% (a total of 27 genes) of the detected grapevine genes were significantly up-regulated by ABA and drought stress, respectively, suggesting that these 27 genes involve in ABA or dehydration stress and may be regulated by VlbZIP30 in grapevine. We propose that VlbZIP30 functions as a positive regulator of drought-responsive signaling in the ABA core signaling pathway.HighlightVlbZIP30 positively regulate plant drought tolerance through regulated the expression of 27 grapevine candidate genes via G-box cis-element (MCACGTGK) in ABA signaling pathway.

scholarly journals A Direct Link between Abscisic Acid Sensing and the Chromatin-Remodeling ATPase BRAHMA via Core ABA Signaling Pathway Components

2016 ◽  
Vol 9 (1) ◽  
pp. 136-147 ◽  
Author(s):  
Marta Peirats-Llobet ◽  
Soon-Ki Han ◽  
Miguel Gonzalez-Guzman ◽  
Cheol Woong Jeong ◽  
Lesia Rodriguez ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Signaling Pathway ◽  
Chromatin Remodeling ◽  
Direct Link ◽  
Aba Signaling

scholarly journals Control of ABA Signaling and Crosstalk with Other Hormones by the Selective Degradation of Pathway Components

2021 ◽  
Vol 22 (9) ◽  
pp. 4638
Author(s):  
Agnieszka Sirko ◽  
Anna Wawrzyńska ◽  
Jerzy Brzywczy ◽  
Marzena Sieńko
Keyword(s):  
Abscisic Acid ◽  
Signaling Pathway ◽  
Plant Hormone ◽  
Aba Signaling ◽  
Degradation Mechanisms ◽  
E3 Ligases ◽  
Selective Autophagy ◽  
Selective Degradation ◽  
Different Parts

A rapid and appropriate genetic and metabolic acclimation, which is crucial for plants’ survival in a changing environment, is maintained due to the coordinated action of plant hormones and cellular degradation mechanisms influencing proteostasis. The plant hormone abscisic acid (ABA) rapidly accumulates in plants in response to environmental stress and plays a pivotal role in the reaction to various stimuli. Increasing evidence demonstrates a significant role of autophagy in controlling ABA signaling. This field has been extensively investigated and new discoveries are constantly being provided. We present updated information on the components of the ABA signaling pathway, particularly on transcription factors modified by different E3 ligases. Then, we focus on the role of selective autophagy in ABA pathway control and review novel evidence on the involvement of autophagy in different parts of the ABA signaling pathway that are important for crosstalk with other hormones, particularly cytokinins and brassinosteroids.

scholarly journals Abscisic Acid Interacts Antagonistically with Salicylic Acid Signaling Pathway in Rice–Magnaporthe grisea Interaction

2010 ◽  
Vol 23 (6) ◽  
pp. 791-798 ◽  
Author(s):  
Chang-Jie Jiang ◽  
Masaki Shimono ◽  
Shoji Sugano ◽  
Mikiko Kojima ◽  
Katsumi Yazawa ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Abscisic Acid ◽  
Signaling Pathway ◽  
Magnaporthe Grisea ◽  
Culture Media ◽  
Blast Resistance ◽  
Aba Signaling ◽  
Host Infection ◽  
Plant Pathogen Interactions ◽  
Sa Signaling

Plant hormones play pivotal signaling roles in plant–pathogen interactions. Here, we report characterization of an antagonistic interaction of abscisic acid (ABA) with salicylic acid (SA) signaling pathways in the rice–Magnaporthe grisea interaction. Exogenous application of ABA drastically compromised the rice resistance to both compatible and incompatible M. grisea strains, indicating that ABA negatively regulates both basal and resistance gene–mediated blast resistance. ABA markedly suppressed the transcriptional upregulation of WRKY45 and OsNPR1, the two key components of the SA signaling pathway in rice, induced by SA or benzothiadiazole or by blast infection. Overexpression of OsNPR1 or WRKY45 largely negated the enhancement of blast susceptibility by ABA, suggesting that ABA acts upstream of WRKY45 and OsNPR1 in the rice SA pathway. ABA-responsive genes were induced during blast infection in a pattern reciprocal to those of WRKY45 and OsPR1b in the compatible rice–blast interaction but only marginally in the incompatible one. These results suggest that the balance of SA and ABA signaling is an important determinant for the outcome of the rice–M. grisea interaction. ABA was detected in hyphae and conidia of M. grisea as well as in culture media, implying that blast-fungus-derived ABA could play a role in triggering ABA signaling at host infection sites.

scholarly journals Novel regulation of transpiration by sugar signals within guard cells

2012 ◽  
Author(s):  
David Granot ◽  
Sarah M. Assmann
Keyword(s):  
Abscisic Acid ◽  
Signaling Pathway ◽  
Water Loss ◽  
Stomatal Closure ◽  
Guard Cells ◽  
Feedback Mechanism ◽  
Limiting Factor ◽  
Aba Signaling ◽  
Sugar Sensing ◽  
New Discovery

Water is the major limiting factor in agriculture and stomata, composed of two guard cells and the pore they circumscribe, are the chief gates controlling plants’ water loss. The prevailing century old paradigm was that sugars act as an osmoticum in guard cells, contributing to the opening of the stomata. In contrast, we discovered that sugars close stomata and the closure is mediated by the sugar-sensing enzyme hexokinase (HXK) that triggers the abscisic acid (ABA)-signaling pathway within the guard cells. This new discovery suggests a sugar-sensing mechanism within guard cells that controls stomatal closure, and supports the existence of a stomatal feedback mechanism that coordinates photosynthesis with transpiration.

scholarly journals ABC transporter AtABCG25 is involved in abscisic acid transport and responses

2010 ◽  
Vol 107 (5) ◽  
pp. 2361-2366 ◽  
Author(s):  
Takashi Kuromori ◽  
Takaaki Miyaji ◽  
Hikaru Yabuuchi ◽  
Hidetada Shimizu ◽  
Eriko Sugimoto ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Abscisic Acid ◽  
Signaling Pathway ◽  
Abc Transporter ◽  
Stress Responses ◽  
Fluorescent Protein ◽  
Plant Cells ◽  
Membrane Vesicles ◽  
Aba Signaling ◽  
Vascular Tissues

Abscisic acid (ABA) is one of the most important phytohormones involved in abiotic stress responses, seed maturation, germination, and senescence. ABA is predominantly produced in vascular tissues and exerts hormonal responses in various cells, including guard cells. Although ABA responses require extrusion of ABA from ABA-producing cells in an intercellular ABA signaling pathway, the transport mechanisms of ABA through the plasma membrane remain unknown. Here we isolated an ATP-binding cassette (ABC) transporter gene, AtABCG25, from Arabidopsis by genetically screening for ABA sensitivity. AtABCG25 was expressed mainly in vascular tissues. The fluorescent protein-fused AtABCG25 was localized at the plasma membrane in plant cells. In membrane vesicles derived from AtABCG25-expressing insect cells, AtABCG25 exhibited ATP-dependent ABA transport. The AtABCG25-overexpressing plants showed higher leaf temperatures, implying an influence on stomatal regulation. These results strongly suggest that AtABCG25 is an exporter of ABA and is involved in the intercellular ABA signaling pathway. The presence of the ABA transport mechanism sheds light on the active control of multicellular ABA responses to environmental stresses among plant cells.

scholarly journals Maize ZmbZIP33 Is Involved in Drought Resistance and Recovery Ability Through an Abscisic Acid-Dependent Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Liru Cao ◽  
Xiaomin Lu ◽  
Guorui Wang ◽  
Qianjin Zhang ◽  
Xin Zhang ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Drought Stress ◽  
Chlorophyll Content ◽  
Signaling Pathway ◽  
Drought Resistance ◽  
Aba Signaling ◽  
Maize Leaves ◽  
Aba Content ◽  
Aba Biosynthesis ◽  
Dependent Signaling Pathway

Analyzing the transcriptome of maize leaves under drought stress and rewatering conditions revealed that transcription factors were involved in this process, among which ZmbZIP33 of the ABSCISIC ACID-INSENSITIVE 5-like protein 5 family was induced to significantly up-regulated. The functional mechanism of ZmbZIP33 in Abscisic acd (ABA) signaling pathway and its response to drought stress and rewatering has not been studied yet. The present study found that ZmbZIP33 contains a DNA-binding and dimerization domain, has transcriptional activation activity, and is highly homologous to SbABI1,SitbZIP68 and OsABA1. The expression of ZmbZIP33 is strongly up-regulated by drought, high salt, high temperature, and ABA treatments. Overexpression of ZmbZIP33 remarkably increased chlorophyll content and root length after drought stress and rewatering, and, moreover, cause an accumulation of ABA content, thereby improving drought resistance and recovery ability in Arabidopsis. However, silencing the expression of ZmbZIP33 (BMV-ZmbZIP33) remarkably decreased chlorophyll content, ABA content, superoxide dismutase and peroxidase activities, and increased stomatal opening and water loss rate compared with BMV (control). It showed that silencing ZmbZIP33 lead to reduced drought resistance and recovery ability of maize. ABA sensitivity analysis found that 0.5 and 1 μmol/L treatments severely inhibited the root development of overexpression ZmbZIP33 transgenic Arabidopsis. However, the root growth of BMV was greatly inhibited for 1 and 5μmol/L ABA treatments, but not for BMV-ZmbZIP33. Subcellular localization, yeast two-hybrid and BIFC further confirmed that the core components of ABA signaling pathways ZmPYL10 and ZmPP2C7 interacted in nucleus, ZmPP2C7 and ZmSRK2E as well as ZmSRK2E and ZmbZIP33 interacted in the plasma membrane. We also found that expression levels of ZmPYL10 and ZmSRK2E in the BMV-ZmbZIP33 mutant were lower than those of BMV, while ZmPP2C7 was the opposite under drought stress and rewatering. However, expression of ZmPYL10 and ZmSRK2E in normal maize leaves were significantly up-regulated by 3–4 folds after drought and ABA treatments for 24 h, while ZmPP2C7 was down-regulated. The NCED and ZEP encoding key enzymes in ABA biosynthesis are up-regulated in overexpression ZmbZIP33 transgenic line under drought stress and rewatering conditions, but down-regulated in BMV-ZmbZIP33 mutants. Together, these findings demonstrate that ZmbZIP33 played roles in ABA biosynthesis and regulation of drought response and rewatering in Arabidopsis and maize thought an ABA-dependent signaling pathway.

scholarly journals Synthesis of Abscisic Acid in Neopyropia yezoensis and Its Regulation of Antioxidase Genes Expressions Under Hypersaline Stress

2022 ◽  
Vol 12 ◽  
Author(s):  
Jiali Yang ◽  
Wenhui Gu ◽  
Zezhong Feng ◽  
Bin Yu ◽  
Jianfeng Niu ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Antioxidant Enzyme ◽  
Signaling Pathway ◽  
Enzyme Activities ◽  
Target Genes ◽  
Higher Plants ◽  
Antioxidant Enzyme Activities ◽  
Aba Signaling ◽  
Exogenous Aba ◽  
Hypersaline Stress

Abscisic acid (ABA) is regarded as crucial for plant adaptation to water-limited conditions and it functions evolutionarily conserved. Thus, insights into the synthesis of ABA and its regulation on downstream stress-responsive genes in Neopyropia yezoensis, a typical Archaeplastida distributed in intertidal zone, will improve the knowledge about how ABA signaling evolved in plants. Here, the variations in ABA contents, antioxidant enzyme activities and expression of the target genes were determined under the presence of exogenous ABA and two specific inhibitors of the ABA precursor synthesis. ABA content was down-regulated under the treatments of each or the combination of the two inhibitors. Antioxidant enzyme activities like SOD, CAT and APX were decreased slightly with inhibitors, but up-regulated when the addition of exogenous ABA. The quantitative assays using real-time PCR (qRT-PCR) results were consistent with the enzyme activities. All the results suggested that ABA can also alleviate oxidative stress in N. yezoensis as it in terrestrial plant. Combined with the transcriptome assay, it was hypothesized that ABA is synthesized in N. yezoensis via a pathway that is similar to the carotenoid pathway in higher plants, and both the MVA and that the MEP pathways for isoprenyl pyrophosphate (IPP) synthesis likely exist simultaneously. The ABA signaling pathway in N. yezoensis was also analyzed from an evolutionary standpoint and it was illustrated that the emergence of the ABA signaling pathway in this alga is an ancestral one. In addition, the presence of the ABRE motif in the promoter region of antioxidase genes suggested that the antioxidase system is regulated by the ABA signaling pathway.

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