scholarly journals Differentiation of Neural Stem Cells into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2

Stem Cells ◽  
2008 ◽  
Vol 26 (11) ◽  
pp. 2875-2883 ◽  
Author(s):  
Falak Sher ◽  
Reinhard Rößler ◽  
Nieske Brouwer ◽  
Veerakumar Balasubramaniyan ◽  
Erik Boddeke ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Group Protein

scholarly journals Polycomb Group Protein Bmi1 Promotes Hematopoietic Cell Development from Embryonic Stem Cells

2012 ◽  
Vol 21 (1) ◽  
pp. 121-132 ◽  
Author(s):  
Xiaolei Ding ◽  
Qiong Lin ◽  
Roberto Ensenat-Waser ◽  
Stefan Rose-John ◽  
Martin Zenke
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cell Development ◽  
Polycomb Group ◽  
Hematopoietic Cell ◽  
Polycomb Group Protein ◽  
Group Protein

Single Cell Level Genome-Wide Gene Expression Analysis of Bone Marrow-Derived Oct-4+ very Small Embryonic-Like Stem Cells (VSELs) Revealed That a Polycomb Group Protein Ezh2 Regulates VSELs Pluripotency by Maintaining Bivalent Domains At Promoters of Important Homeodomain-Containing Developmental Transcription Factors

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 2345-2345
Author(s):  
Magda Kucia ◽  
Rui Liu ◽  
Kasia Mierzejewska ◽  
Wan Wu ◽  
Janina Ratajczak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Transcription Factors ◽  
Single Cell ◽  
Gene Expression Analysis ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Genome Wide ◽  
Group Protein ◽  
Genome Wide Gene Expression

Abstract Abstract 2345 Recently, we identified a population of very small embryonic-like (VSEL) stem cells (SCs) in adult bone marrow (BM) (Leukemia 2006:20;857). These Oct4+CXCR4+SSEA-1+Sca-1+CD45−Lin− VSELs are capable of differentiation in vitro into cells from all three germ lineages and in in vivo animal models they can be specified into mesenchymal stem cells (MSCs) (Stem Cells Dev 2010:19;1557), cardiomyocytes (Stem Cell 2008:26;1646), and long-term engrafting hematopoietic stem cells (HSCs) (Exp Hematol 2011:39;225). Be employing gene-expression and epigenetic profiling studies we reported that VSELs in BM have germ-line stem cell like epigenetic features including i) open/active chromatin structure in Oct4 promoter, ii) parent-of-origin specific reprogramming of genomic imprinting (Leukemia 2009, 23, 2042–2051), and iii) that they share several markers with epiblast-derived primordial germ cells (PGCs), in particular with migratory PGCs (Leukemia 2010, 24, 1450–1461). However, it was not clear how VSELs maintain pluripotent state. To address this issue we recently employed single cell-based genome-wide gene expression analysis and found that, Oct4+ VSELs i) express a similar, yet nonidentical, transcriptome as embryonic stem-cells (ESCs), ii) up-regulate cell-cycle checkpoint genes, and iii) down-regulate genes involved in protein turnover and mitogenic pathways. Interestingly, our single cell library studies also revelaed that Ezh2, a polycomb group protein, is highly expressed in VSELs. This protein is well known to be involved in maintaining a bivalent domains (BDs) at promoters of important homeodomain-containing developmental transcription factors. Of note a presence of BDs is characteristic for pluripotent stem cells (e.g., ESCs) and as result of Ezh2 overexpression, VSELs, like ESCs, exhibit BDs - bivalently modified nucleosomes (trimethylated H3K27 and H3K4) at promoters of important homeodomain-containing developmental transcription factors (Sox21 Nkx2.2 Dlx1 Zfpm2 Irx2 Lbx1h Hlxb9 Pax5 HoxA3). Of note, spontaneous (as seen during differentiation) or RNA interference-enforced down-regulation of Ezh2 removes BDs what, results in lose of their plurioptentiality and de-repression of several BD-regulated genes that control their tissue commitment. In conclusion, Our results show for first time that in addition to the expression of pluripotency core transcription factor Oct-4, VSELs, like other pluripotent stem-cells, maintain their pluripotent state through an Ezh2-dependent BD-mediated epigenetic mechanism. Based on this our genome-wide gene expression study not only advances our understanding of biological processes that govern VSELs pluripotency, differentiation, and quiescence but will also help to develop better protocols for ex vivo expansion of these promising cells for potential application in regenerative medicine. Disclosures: Ratajczak: Neostem Inc: Consultancy, Research Funding.

scholarly journals The Polycomb Group Protein L3MBTL1 Represses a SMAD5-Mediated Hematopoietic Transcriptional Program in Human Pluripotent Stem Cells

2015 ◽  
Vol 4 (4) ◽  
pp. 658-669 ◽  
Author(s):  
Fabiana Perna ◽  
Ly P. Vu ◽  
Maria Themeli ◽  
Sonja Kriks ◽  
Ruben Hoya-Arias ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Transcriptional Program ◽  
Group Protein

scholarly journals Retinoic Acid Receptor Isotype Specificity in F9 Teratocarcinoma Stem Cells Results from the Differential Recruitment of Coregulators to Retinoic Acid Response Elements

2007 ◽  
Vol 282 (46) ◽  
pp. 33421-33434 ◽  
Author(s):  
Robert F. Gillespie ◽  
Lorraine J. Gudas
Keyword(s):  
Stem Cells ◽  
Retinoic Acid ◽  
Target Genes ◽  
Retinoic Acid Receptor ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Response Elements ◽  
Acid Receptor ◽  
Group Protein ◽  
F9 Teratocarcinoma

The retinoic acid receptor (RAR) α, β2, and γ isotypes each regulate specific subsets of target genes in F9 teratocarcinoma stem cells. We used chromatin immunoprecipitation assays to monitor the association of RARγ, retinoic X receptor (RXR) α, and coregulators with the RARβ2, Hoxa1, and Cyp26A1 retinoic acid response elements (RAREs) in F9 wild type and RARα, -β2, and -γ null cells. Additionally we quantitatively monitored expression of the corresponding mRNAs. We demonstrated that the association of RARγ and/or RXRα with a RARE was not sufficient for retinoic acid (RA)-mediated transcription of the corresponding target gene. However, the ability of RARγ and/or RXRα to recruit pCIP (AIB1/ACTR/RAC-3/TRAM-1/SRC-3) and p300 to a RARE did correlate with RA-associated transcription of target mRNAs. Therefore, the specific functions of the RAR isotypes do not manifest at the level of their DNA binding but rather from a differential ability to recruit specific components of the transcriptional machinery. We also demonstrated that RA-mediated displacement of the polycomb group protein SUZ12 from a RARE was inhibited in the absence of RARγ. Thus, transcriptional components of the RAR signaling pathway are specifically required for displacement of SUZ12 from RAREs during RA-mediated differentiation of F9 cells.

scholarly journals The polycomb group protein Yaf2 regulates the pluripotency of embryonic stem cells in a phosphorylation-dependent manner

2018 ◽  
Vol 293 (33) ◽  
pp. 12793-12804 ◽  
Author(s):  
Wukui Zhao ◽  
Mengjie Liu ◽  
Haijing Ji ◽  
Yaru Zhu ◽  
Congcong Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Polycomb Group ◽  
Dependent Manner ◽  
Polycomb Group Protein ◽  
Group Protein

scholarly journals Survival of skin cancer stem cells requires the Ezh2 polycomb group protein

2015 ◽  
Vol 36 (7) ◽  
pp. 800-810 ◽  
Author(s):  
Gautam Adhikary ◽  
Daniel Grun ◽  
Sivaprakasam Balasubramanian ◽  
Candace Kerr ◽  
Jennifer M. Huang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Skin Cancer ◽  
Cancer Stem Cells ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Group Protein

scholarly journals Loss of Polycomb Group Protein Pcgf1 Severely Compromises Proper Differentiation of Embryonic Stem Cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yun Yan ◽  
Wukui Zhao ◽  
Yikai Huang ◽  
Huan Tong ◽  
Yin Xia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Group Protein

scholarly journals The Ezh2 polycomb group protein drives an aggressive phenotype in melanoma cancer stem cells and is a target of diet derived sulforaphane

2015 ◽  
Vol 55 (12) ◽  
pp. 2024-2036 ◽  
Author(s):  
Matthew L. Fisher ◽  
Gautam Adhikary ◽  
Dan Grun ◽  
David M. Kaetzel ◽  
Richard L. Eckert
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Polycomb Group ◽  
Polycomb Group Protein ◽  
Aggressive Phenotype ◽  
Melanoma Cancer ◽  
Group Protein
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