THE EFFECTS OF INGESTION OF HYPERTONIC SALINE ON THE PITUITARY GLAND IN THE RAT: A MORPHOLOGICAL STUDY OF THE PARS INTERMEDIA AND POSTERIOR LOBE

1962 ◽  
Vol 25 (2) ◽  
pp. 161-NP ◽  
Author(s):  
L. W. DUCHEN
Keyword(s):  
Sodium Chloride ◽  
Pituitary Gland ◽  
Mitotic Activity ◽  
Hypertonic Saline ◽  
Morphological Study ◽  
Tap Water ◽  
Neurosecretory Material ◽  
Pars Intermedia ◽  
Posterior Lobe ◽  
Neurohypophysial Hormones

SUMMARY The microscopic changes occurring in the pars intermedia and posterior lobe of the pituitary gland were studied in rats given 2% sodium chloride in place of tap water for periods ranging from 1 to 21 days. In the pars intermedia small, strongly PAS-positive cells which are normally few in number became very numerous during the first few days of treatment with saline. After longer periods on saline these changes appeared to regress. Mitotic activity in the pars intermedia and posterior lobe was studied using colchicine. Mitoses were present in the pars intermedia of control rats but not in that of salt-loaded rats. In the posterior lobe mitoses were not present in control rats but were numerous in rats drinking saline, and the greatest numbers were seen after 4 days. The neurosecretory material (NSM) was lost from the posterior lobe within the first 8 days, and it is suggested that there is a relationship between the loss of NSM and mitotic activity in the posterior lobe. The significance of the changes in the pars intermedia is not apparent at present but may be related to the removal of neurohypophysial hormones.

CHANGES IN THE VOLUME OF THE LOBES OF THE PITUITARY GLAND AND IN THE WEIGHT AND WATER CONTENT OF ORGANS OF RATS GIVEN HYPERTONIC SALINE

1968 ◽  
Vol 41 (4) ◽  
pp. 593-600 ◽  
Author(s):  
L. W. DUCHEN
Keyword(s):  
Body Weight ◽  
Sodium Chloride ◽  
Pituitary Gland ◽  
Water Content ◽  
Hypertonic Saline ◽  
Marked Reduction ◽  
Anterior Lobe ◽  
Pars Intermedia ◽  
Posterior Lobe

SUMMARY The volume of the various lobes of the pituitary gland were measured in rats which were given 2% sodium chloride to drink instead of water. The volume of the posterior lobe was increased by 60% after 11 days on saline. The volume of the anterior lobe was slightly decreased but the volume of the pars intermedia was not changed. There was a marked loss of body weight in animals which were given saline and a marked reduction in the weight of the whole pituitary, liver and thyroid.

EFFECTS OF PIMOZIDE AND BROMOCRIPTINE ON THE PROLIFERATION OF RAT PITUITARY PARS INTERMEDIA CELLS

1977 ◽  
Vol 75 (3) ◽  
pp. 443-444 ◽  
Author(s):  
J. RYCHTER ◽  
H. STEPIEŃ
Keyword(s):  
Pituitary Gland ◽  
Dopamine Receptor ◽  
Mitotic Activity ◽  
Secretory Cells ◽  
Pars Intermedia ◽  
Dopamine Receptor Agonist ◽  
Intermediate Lobe ◽  
Melanocyte Stimulating Hormone ◽  
Medical Academy ◽  
Rat Pituitary

Department of Experimental Endocrinology, Institute of Endocrinology, Medical Academy of Łódź, Dr. Sterling str. 3, 91-425 Łódź, Poland (Received 31 May 1977) The secretory function of the intermediate lobe of the pituitary gland is under hypothalamic control (Howe, 1973; Hadley & Bagnara, 1975). Penny, Thody, Tilders & Smelik (1977) have suggested that the synthesis and release of melanocyte-stimulating hormone (MSH) is mediated by dopaminergic neurones which make synaptic contact with secretory cells in the pars intermedia (Björklund, Moore, Nobin & Stenevi, 1973). We have attempted to examine whether the dopaminergic mechanism is also involved in the control of the mitotic activity of the pars intermedia cells and have studied the effects of pimozide, a dopamine receptor blocker (Anden, Butcher, Corrodi, Fuxe & Ungerstedt, 1970) and 2-bromo-α-ergocriptine (bromo-criptine), a dopamine receptor agonist (Loew, Vigouret & Jaton, 1976) on the mitotic activity of the pars intermedia of the rat pituitary gland. Twenty-three

THE PITUITARY GLAND OF THE FEMALE FERRET

1960 ◽  
Vol 20 (1) ◽  
pp. 48-55 ◽  
Author(s):  
R. L. HOLMES
Keyword(s):  
Pituitary Gland ◽  
Alcian Blue ◽  
Periodic Acid ◽  
Performic Acid ◽  
Neurosecretory Material ◽  
Pars Intermedia ◽  
Pars Tuberalis ◽  
Neural Process ◽  
Positive Material ◽  
Orange G

SUMMARY Six distinct types of cell can be distinguished in the pars distalis of the pituitary gland of the female ferret after staining by performic acid-Alcian blue followed by periodic acid-Schiff (PAS)-orange G. The first of these types contains coarsely granular PAS-positive material; the second contains granules which stain with Alcian blue, with or without PAS-positive material; while the third contains finely granular or diffuse PAS-positive material. The fourth type of cell, apparently equivalent to the 'carminophil' of other species, stains deep orange due to a combination of staining with orange G and a weakly positive PAS reaction. The fifth type is the ordinary acidophil, and the sixth the chromophobe. The pars tuberalis is extensive, and may be compact or lobulated. The pars intermedia contains two types of cell, but shows no obvious secretory characteristics. The neural process consists of a central zone which resembles the stalk in structure, and a peripheral, more vascular zone which contains the bulk of the neurosecretory material. A nucleus of small nerve cells lies in the junctional region where the stalk joins the main neural process.

Isolation from the posterior lobe of the pituitary gland of a protein specifically but loosely binding oxytocin and vasopressin

1968 ◽  
Vol 170 (1018) ◽  
pp. 3-5 ◽  
Keyword(s):  
Pituitary Gland ◽  
Medical Research ◽  
Special Interest ◽  
Peking Union Medical College ◽  
Pars Intermedia ◽  
Posterior Lobe ◽  
Columbia University ◽  
Medical College ◽  
The University ◽  
Important Constituent

It is indeed a pleasure to write, in response to the invitation of Dr Blaschko, a brief background account of early work on the protein which is an important constituent of the posterior lobe of the pituitary. My special interest in this complex gland is a debt, contracted more than 40 years ago, to Paul Trendelenburg, in whose active laboratory in Freiburg i. B. I investigated the distribution of oxytocic, vasopressor and antidiuretic activities in the neurohypophysis of the ox (van Dyke 1926). Subsequent research dealt with various aspects of the biochemistry and physiology of the principal divisions of the pituitary: the pars glandularis, the pars intermedia and the pars neuralis while I worked as a pharmacologist in the University of Chicago, in the Peking Union Medical College, in the Squibb Institute for Medical Research and in the College of Physicians and Surgeons of Columbia University.

REPLETION OF VASOPRESSIN AND OXYTOCIN IN THE POSTERIOR LOBE OF THE PITUITARY GLAND OF THE RAT

1968 ◽  
Vol 40 (3) ◽  
pp. 337-342 ◽  
Author(s):  
T. K. YOUNG ◽  
H. B. VAN DYKE
Keyword(s):  
Drinking Water ◽  
Pituitary Gland ◽  
Free Access ◽  
Posterior Lobe ◽  
Neurohypophysial Hormones ◽  
Depletion Rate ◽  
Access To Water ◽  
The Mean

SUMMARY Rats deprived of drinking water for 7 days showed a striking depletion of neurohypophysial hormones from the posterior lobe of the pituitary gland. The average daily depletion rate was estimated to be 93 m-u. for vasopressin and 97 m-u. for oxytocin. When rats were allowed free access to water, dehydration was rapidly corrected as shown by normal haematocrit values and plasma osmolarities. Repletion of neurohypophysial hormones, rapid in the first 24 hr., continued gradually thereafter. The mean calculated repletion rate was 41 m-u./day for vasopressin and 42 m-u./day for oxytocin. Repletion was completed about 14 days after rehydration.

DIRECT ANTIPROLIFERATIVE EFFECT OF DOPAMINE AGONISTS ON THE ANTERIOR PITUITARY GLAND IN ORGAN CULTURE

1978 ◽  
Vol 79 (2) ◽  
pp. 245-246 ◽  
Author(s):  
M. PAWLIKOWSKI ◽  
J. KUNERT-RADEK ◽  
H. STĘPIEŃ
Keyword(s):  
Pituitary Gland ◽  
Dopamine Receptor ◽  
Mitotic Activity ◽  
Anterior Pituitary ◽  
Anterior Pituitary Gland ◽  
Antiproliferative Effect ◽  
Male Rats ◽  
Pars Intermedia ◽  
Pituitary Tumours ◽  
Rat Pituitary

Department of Experimental Endocrinology, Institute of Endocrinology, Medical Academy of Łódź, Dr Sterling sir. 3, 91–425 Łódź, Poland (Received 22 May 1978) Lloyd, Meares & Jacobi (1975) observed inhibition of mitotic activity in the anterior pituitary gland by the dopamine receptor agonist, bromocriptine, in oestrogen-treated male rats. This observation has been confirmed in our laboratory (Stępień, Wolaniuk & Pawlikowski, 1978). Suppression of mitotic activity in the pars intermedia of the rat pituitary gland by bromocriptine has also been observed (Rychter & Stępień, 1977). Furthermore, it has been found that the dopamine receptor blocker, pimozide, enhances mitotic activity in the rat anterior pituitary gland (Stępień et al. 1978). By the use of various ergot alkaloids, MacLeod & Lehmeyer (1973) succeeded in inhibiting the growth of transplantable rat pituitary tumours. There have also been observations suggesting an antiproliferative effect of bromocriptine on human pituitary tumours (Wass, Thorner, Morris, Rees, Mason, Jones &

The functional significance of glycosylation of proopiomelanocortin in melanotrophs of the mouse pituitary gland

1985 ◽  
Vol 107 (3) ◽  
pp. 365-374 ◽  
Author(s):  
B. G. Jenks ◽  
A. G. H. Ederveen ◽  
J. H. M. Feyen ◽  
A. P. van Overbeeke
Keyword(s):  
Pituitary Gland ◽  
Functional Significance ◽  
Peptide Hormones ◽  
Terminal Region ◽  
Pars Intermedia ◽  
Apparent Effect ◽  
Glycoprotein Precursor ◽  
Tunicamycin Treatment ◽  
Mouse Pituitary

ABSTRACT Pro-opiomelanocortin (POMC) is a glycoprotein precursor for a number of neuropeptides and peptide hormones. The functional significance of the glycosylation of POMC has never been established. Using the antibiotic tunicamycin to block glycosylation of the prohormone in the mouse pars intermedia, we have compared processing of non-glycosylated prohormone with that of glycosylated prohormone in pulse-chase experiments. The peptides produced from non-glycosylated prohormone were shown to be correct cleavage products. Therefore it was concluded that, with the possible exception of peptides from the N-terminal region of the prohormone, the carbohydrate on POMC plays no role in directing cleavage or in protecting the prohormone from random proteolysis. Tunicamycin treatment retarded N-terminal acetylation of melanotrophin but had no apparent effect on acetylation of β-endorphin. The mouse pars intermedia synthesizes two forms of POMC which differ in their degree of glycosylation. Our results indicated that, during secretion, the melanotrophs make no distinction between peptides derived from the two prohormones. J. Endocr. (1985) 107, 365–374

scholarly journals The oxytocic hormone of the posterior lobe of the pituitary gland

1935 ◽  
Vol 29 (2) ◽  
pp. 378-390.2 ◽  
Author(s):  
John Masson Gulland ◽  
Sydney Stewart Randall
Keyword(s):  
Pituitary Gland ◽  
Posterior Lobe

On the content of prolan in the pituitary gland

1934 ◽  
Vol 30 (6) ◽  
pp. 634-634
Author(s):  
P. Badul
Keyword(s):  
Pituitary Gland ◽  
Histological Examination ◽  
Anterior Lobe ◽  
Posterior Lobe ◽  
Posterior Pituitary ◽  
Posterior Pituitary Lobe

The posterior lobe of the pituitary gland in a bull is free of prolan, while in a human it contains prolan. Only here it can be found in that part of the posterior pituitary lobe adjacent to the anterior lobe. In the bull, too, this part of the pituitary gland is completely free of prolan content. Histological examination shows that in humans, this part of the posterior lobe is crossed by bands of cells from the anterior lobe, which consist exclusively of basophilic cells.

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