EFFECT OF PROGESTERONE ON SURVIVAL OF BLASTOCYSTS IN UTERI OF OVARIECTOMIZED MICE

1971 ◽  
Vol 51 (2) ◽  
pp. 375-NP ◽  
Author(s):  
H. M. WEITLAUF
Keyword(s):  
Time Interval ◽  
Ovariectomized Mice ◽  
Vaginal Plug

SUMMARY Mice were ovariectomized on day 4 of pregnancy and injected daily with either progesterone or oil vehicle (day 1 = the day of vaginal plug). Blastocysts were recovered on day 10, 20 or 30, counted and transferred to ovariectomized recipients. Implantation was induced and pregnancy maintained by daily injections of oestradiol and progesterone. Viability of the blastocysts was assessed by determining the percentage that developed into normal foetuses. A similar number of eggs was recovered from both groups at each time-interval; thus progesterone did not influence the retention of blastocysts by ovariectomized mice. In contrast, progesterone did influence the viability of the embryos. The percentage of blastocysts developing normally from the oil-treated animals was: 39% on day 10, 19% on day 20 and less than 1% on day 30. Corresponding percentages for the progesterone-treated animals were: 40% on day 10, 35% on day 20 and 35% on day 30.

ENDOCRINE CONTROL OF ENDOMETRIAL SENSITIVITY DURING THE INDUCTION OF THE DECIDUAL CELL REACTION IN THE MOUSE

1966 ◽  
Vol 36 (3) ◽  
pp. 239-248 ◽  
Author(s):  
C. A. FINN
Keyword(s):  
Time Interval ◽  
Decidual Cell ◽  
Cell Reaction ◽  
Endocrine Control ◽  
Optimum Time ◽  
Ovariectomized Mice

SUMMARY The decidual cell reaction (DCR) of ovariectomized mice treated with hormones and stimulated by the intrauterine injection of oil was investigated to obtain information about the control of uterine sensitivity during implantation. Three factors were studied; oestrogen given at the time of oestrus, progesterone and oestrogen given at the time of the nidatory surge. For the induction of the DCR by oil both 'oestrous' oestrogen and 'nidatory surge' oestrogen were essential, whereas neither were necessary for the traumatic DCR. The quantity of nidatory surge oestrogen was very critical; 0·01 μg. were effective, 0·0625 μg. inhibitory. There was no quantitative interaction between nidatory surge oestrogen and progesterone, indicating that in this situation the two hormones are acting independently. The optimum time interval between initiating the oestrogen surge and injecting the oil was between 4 and 8 hr. No response was obtained when the oestrogen surge was produced and the stimulus applied on the second day of treatment with progesterone; maximal responses were obtained on the fourth and fifth day and a reduced response on the seventh day. After an oestrogen surge on the fourth day, it was not possible to elicit an oil DCR to a further dose of oestrogen on the fifth day, indicating that the period of sensitivity induced by the surge on the fourth day is followed by a period of refractoriness.

scholarly journals EFEK EKSTRAK TESTIS TERHADAP JUMLAH IMPLANTASI DAN JUMLAH ANAK PADA MENCIT (Mus musculus)

2004 ◽  
Vol 10 (1) ◽  
pp. 67-70
Author(s):  
Sri Puji Astuti Wahyuningsih ◽  
Listijani Suhargo ◽  
Kushendarsasi Kushendarsasi
Keyword(s):  
Immune Responses ◽  
Mus Musculus ◽  
Effective Concentration ◽  
Time Interval ◽  
Male Mice ◽  
Random Design ◽  
Vaginal Plug ◽  
Specific Proteins

Extract of testis contains the testis specific proteins. The testis proteins may raise immune responses. The immune responses are the antibodies against testis specific anti-protein. The reaction between the antibodies and the sperms can inhibit fertilization. This process has contraception effects. This research used 24 female and 20 male mice strain Balb/c. The experiment used the complete random design with 4 concentration treatments (0, 500, 1000, and 2000 μg extracts of testis) and 3 replications. Mice were injected 3 times with time interval 21 days. After 15 days from the rising of vaginal plug, 3 mice were dissected and counted the implantation. The others mice were waited until delivered of the children. The datas were analyzed by ANOVA and LSD (a = 5 percent). The results showed that extract of testis did not influence the amount of implantation at the uterus endometrial, but it decreased the amount of children. The effective concentration of extract testis is 2000 μg. So, this study showed that the intraperitoneum injection of the extract of testis had contraception effects.

scholarly journals Secreted phosphoprotein 1 (osteopontin) is expressed by stromal macrophages in cyclic and pregnant endometrium of mice, but is induced by estrogen in luminal epithelium during conceptus attachment for implantation

Reproduction ◽  
2006 ◽  
Vol 132 (6) ◽  
pp. 919-929 ◽  
Author(s):  
Frankie J White ◽  
Robert C Burghardt ◽  
Jianbo Hu ◽  
Margaret M Joyce ◽  
Thomas E Spencer ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Immune Cells ◽  
Luminal Epithelium ◽  
Apical Surface ◽  
Wild Type ◽  
Mouse Uterus ◽  
Ovariectomized Mice ◽  
Vaginal Plug ◽  
Secreted Phosphoprotein 1

Secreted phosphoprotein 1 (SPP1, osteopontin) is the most highly upregulated extracellular matrix/adhesion molecule/cytokine in the receptive phase human uterus, and Spp1 null mice manifest decreased pregnancy rates during mid-gestation as compared with wild-type counterparts. We hypothesize that Spp1 is required for proliferation, migration, survival, adhesion, and remodeling of cells at the conceptus–maternal interface. Our objective was to define the temporal/spatial distribution and steroid regulation of Spp1 in mouse uterus during estrous cycle and early gestation.In situhybridization localizedSpp1to luminal epithelium (LE) and immune cells. LE expression was prominent at proestrus, decreased by estrus, and was nearly undetectable at diestrus. During pregnancy,Spp1mRNA was not detected in LE until day 4.5 (day 1 = vaginal plug).Spp1-expressing immune cells were scattered within the endometrial stroma throughout the estrous cycle and early pregnancy. Immunoreactive Spp1 was prominent at the apical LE surface by day 4.5 of pregnancy and Spp1 protein was also co-localized with subsets of CD45-positive (leukocytes) and F4/80-positive (macrophages) cells. In ovariectomized mice, estrogen, but not progesterone, inducedSpp1mRNA, whereas estrogen plus progesterone did not induceSpp1in LE. These results establish that estrogen regulates Spp1 in mouse LE and are the first to identify macrophages that produce Spp1 within the peri-implantation endometrium of any species. We suggest that Spp1 at the apical surface of LE provides a mechanism to bridge conceptus to LE during implantation, and that Spp1-positive macrophages within the stroma may be involved in uterine remodeling for conceptus invasion.

Focusing of Visuospatial Attention

2001 ◽  
Vol 15 (4) ◽  
pp. 256-274 ◽  
Author(s):  
Caterina Pesce ◽  
Rainer Bösel
Keyword(s):  
Reaction Times ◽  
Visual Space ◽  
Spatial Relation ◽  
Stimulus Onset ◽  
Visuospatial Attention ◽  
Task Demands ◽  
Time Interval ◽  
Behavioral Studies ◽  
Task Conditions ◽  
Simple Rt

Abstract In the present study we explored the focusing of visuospatial attention in subjects practicing and not practicing activities with high attentional demands. Similar to the studies of Castiello and Umiltà (e. g., 1990) , our experimental procedure was a variation of Posner's (1980) basic paradigm for exploring covert orienting of visuospatial attention. In a simple RT-task, a peripheral cue of varying size was presented unilaterally or bilaterally from a central fixation point and followed by a target at different stimulus-onset-asynchronies (SOAs). The target could occur validly inside the cue or invalidly outside the cue with varying spatial relation to its boundary. Event-related brain potentials (ERPs) and reaction times (RTs) were recorded to target stimuli under the different task conditions. RT and ERP findings showed converging aspects as well as dissociations. Electrophysiological results revealed an amplitude modulation of the ERPs in the early and late Nd time interval at both anterior and posterior scalp sites, which seems to be related to the effects of peripheral informative cues as well as to the attentional expertise. Results were: (1) shorter latency effects confirm the positive-going amplitude enhancement elicited by unilateral peripheral cues and strengthen the criticism against the neutrality of spatially nonpredictive peripheral cueing of all possible target locations which is often presumed in behavioral studies. (2) Longer latency effects show that subjects with attentional expertise modulate the distribution of the attentional resources in the visual space differently than nonexperienced subjects. Skilled practice may lead to minimizing attentional costs by automatizing the use of a span of attention that is adapted to the most frequent task demands and endogenously increases the allocation of resources to cope with less usual attending conditions.

Attention Deficit and Disruptive Behavior Disorder Symptoms

2001 ◽  
Vol 17 (1) ◽  
pp. 25-35 ◽  
Author(s):  
G. Leonard Burns ◽  
James A. Walsh ◽  
David R. Patterson ◽  
Carol S. Holte ◽  
Rita Sommers-Flanagan ◽  
...  
Keyword(s):  
Attention Deficit ◽  
Rating Scales ◽  
Behavior Disorder ◽  
Disruptive Behavior Disorder ◽  
Subjective Rating ◽  
Time Interval ◽  
Assessment Procedure ◽  
Frequency Count ◽  
Hyperactivity Disorder ◽  
Oppositional Defiant

Summary: Rating scales are commonly used to measure the symptoms of attention deficit/hyperactivity disorder (ADHD), oppositional defiant disorder (ODD), and conduct disorder (CD). While these scales have positive psychometric properties, the scales share a potential weakness - the use of vague or subjective rating procedures to measure symptom occurrence (e. g., never, occasionally, often, and very often). Rating procedures based on frequency counts for a specific time interval (e. g., never, once, twice, once per month, once per week, once per day, more than once per day) are less subjective and provide a conceptually better assessment procedure for these symptoms. Such a frequency count procedure was used to obtain parent ratings on the ADHD, ODD, and CD symptoms in a normative (nonclinical) sample of 3,500 children and adolescents. Although the current study does not provide a direct comparison of the two types of rating procedures, the results suggest that the frequency count procedure provides a potentially more useful way to measure these symptoms. The implications of the results are noted for the construction of rating scales to measure the ADHD, ODD, and CD symptoms.

An Adaptive and Learning System for Feedback-Controlled Evoked Response Studies

1981 ◽  
Vol 20 (03) ◽  
pp. 169-173
Author(s):  
J. Wagner ◽  
G. Pfurtscheixer
Keyword(s):  
Brain Activity ◽  
Evoked Response ◽  
Learning System ◽  
Time Interval ◽  
Electrical Brain Activity ◽  
Long Time ◽  
The Subject ◽  
Eeg Power ◽  
Stimulation System ◽  
Background Eeg

The shape, latency and amplitude of changes in electrical brain activity related to a stimulus (Evoked Potential) depend both on the stimulus parameters and on the background EEG at the time of stimulation. An adaptive, learnable stimulation system is introduced, whereby the subject is stimulated (e.g. with light), whenever the EEG power is subthreshold and minimal. Additionally, the system is conceived in such a way that a certain number of stimuli could be given within a particular time interval. Related to this time criterion, the threshold specific for each subject is calculated at the beginning of the experiment (preprocessing) and adapted to the EEG power during the processing mode because of long-time fluctuations and trends in the EEG. The process of adaptation is directed by a table which contains the necessary correction numbers for the threshold. Experiences of the stimulation system are reflected in an automatic correction of this table. Because the corrected and improved table is stored after each experiment and is used as the starting table for the next experiment, the system >learns<. The system introduced here can be used both for evoked response studies and for alpha-feedback experiments.

Effect of Dipyridamole on Spontaneous Platelet Aggregation in Whole Blood Decreases with the Time After Venepuncture: Evidence for the Role of ADP

1987 ◽  
Vol 58 (02) ◽  
pp. 744-748 ◽  
Author(s):  
A R Saniabadi ◽  
G D O Lowe ◽  
J C Barbenel ◽  
C D Forbes
Keyword(s):  
Platelet Aggregation ◽  
Correlation Coefficient ◽  
Whole Blood ◽  
Blood Platelet ◽  
Inhibitory Effect ◽  
Time Interval ◽  
Adp Receptor ◽  
Spontaneous Platelet Aggregation

SummarySpontaneous platelet aggregation (SPA) was studied in human whole blood at 3, 5, 10, 20, 30, 40 and 60 minutes after venepuncture. Using a whole blood platelet counter, SPA was quantified by measuring the fall in single platelet count upon rollermixing aliquots of citrated blood at 37° C. The extent of SPA increased with the time after venepuncture, with a correlation coefficient of 0.819. The inhibitory effect of dipyridamole (Dipy) on SPA was studied: (a) 10 μM at each time interval; (b) 0.5-100 μM at 3 and 30 minutes and (c) 15 μM in combination with 100 μM adenosine, 8 μM 2-chloroadenosine (2ClAd, an ADP receptor blocker) and 50 μM aspirin. There was a rapid decrease in the inhibitory effect of Dipy with the time after venepuncture; the correlation coefficient was -0.533. At all the concentrations studied, Dipy was more effective at 3 minutes than at 30 minutes after venepuncture. A combination of Dipy with adenosine, 2ClAd or aspirin was a more effective inhibitor of SPA than either drug alone. However, when 15 μM Dipy and 10 μM Ad were added together, the inhibitory effect of Dipy was not increased significantly, suggesting that Dipy inhibits platelet aggregation independent of Ad. The increase in SPA with the time after venepuncture was abolished when blood was taken directly into the anticoagulant containing 5 μM 2ClAd. It is suggested that ADP released from the red blood cells is responsible for the increased platelet aggregability with the time after venepuncture and makes a serious contribution to the artifacts of in vitro platelet function studies.

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