EFFECT OF PROGESTERONE ON COLLAGEN BREAKDOWN AND TISSUE COLLAGENOLYTIC ACTIVITY IN THE INVOLUTING RAT UTERUS

1975 ◽  
Vol 66 (3) ◽  
pp. 357-362 ◽  
Author(s):  
JOUKO HALME ◽  
J. F. WOESSNER
Keyword(s):  
Post Partum ◽  
Collagenolytic Activity ◽  
Rat Uterus ◽  
Uterine Involution ◽  
Wet Weight

SUMMARY Progestational agents were studied for their effects on collagenolytic activity and loss of collagen and wet weight from the involuting post-partum rat uterus. Administration of very large doses of progesterone (80–150 mg/day) significantly retarded uterine involution and loss of collagen. This was accompanied by a significant reduction in uterine collagenolytic activity. By 72 h post partum, uteri of rats treated with 150 mg progesterone/day had wet weights 30% above, collagen 85% above, and collagenolytic activity 45% below, those of the control uteri. Similar effects were produced by 17α-acetoxy-6α-methylprogesterone at the same dosage levels. However, the progestational agent 6α-chloro-17α-acetoxypregna-4,6-diene-3,20dione acetate had no effect in this system.

Increase in plasminogen activator in the involuting uterus of the postpartum rat

1985 ◽  
Vol 104 (2) ◽  
pp. 295-298 ◽  
Author(s):  
H. Shimada ◽  
H. Okamura ◽  
L. L. Espey ◽  
T. Mori
Keyword(s):  
Plasminogen Activator ◽  
Indirect Method ◽  
Post Partum ◽  
Control Level ◽  
Collagenolytic Activity ◽  
Tissue Remodelling ◽  
Rat Uterus ◽  
Wet Weight ◽  
Plasmin Inhibitors ◽  
Hydrolysis Of

ABSTRACT Plasminogen activator (PA) activity in the rat uterus was measured at fixed intervals post partum in order to determine whether this serine protease increases during the acute remodelling of tissue which occurs in the involuting uterus. Plasminogen activator activity was measured by an indirect method based on the hydrolysis of the chromogenic substrate S-2251 by PA-generated plasmin. At the time of parturition the control level of PA activity was 0·033 ± 0·018 (s.d.) μmol/4 mg uterine wet weight per 30 min. This activity increased fourfold to a peak of 0·131 ±0·036 at 3 days post partum, and then it declined steadily towards the control level during the next 7 days. Concomitantly, uterine weight decreased to 25% of the control weight by 3 days post partum, and it continued to decrease until day 15. In the 30 days post partum during which PA activity was monitored there was no significant change in plasmin inhibitors in the uterine extracts. The results suggest a correlation between PA activity and the process of tissue remodelling which occurs during involution of the rat uterus. This increase in PA might serve to activate a latent collagenase since the measured peak in PA activity happens to coincide with a reported increase in collagenolytic activity in the involuting rat uterus. J. Endocr. (1985) 104, 295–298

EFFECT OF OESTRADIOL ON THE POST-PARTUM RAT UTERUS: PEROXIDASE ACTIVITY AND COLLAGEN BREAKDOWN

1980 ◽  
Vol 85 (3) ◽  
pp. 387-391 ◽  
Author(s):  
J. F. WOESSNER ◽  
JANET N. RYAN
Keyword(s):  
Epithelial Cells ◽  
Peroxidase Activity ◽  
Post Partum ◽  
Cell Type ◽  
Rat Uterus ◽  
Uterine Epithelial Cells ◽  
Uterine Involution ◽  
Tenfold Increase

Treatment of parturient rats with 100 μg oestradiol/day caused a significant retardation of uterine involution and collagen breakdown. The post-partum uterus had a peroxidase activity of 180 μmol H2O2 reduced/min per uterus. Treatment with oestradiol caused an eight- to tenfold increase in this activity within 3–4 days. Treatment of rats with 4 mg cortisol/day commencing 3 days prepartum had no effect on uterine peroxidase activity but it blocked the oestradiol-induced increase in peroxidase. Cortisol had no effect on collagen breakdown and did not reverse the inhibition of collagen breakdown produced by oestradiol. It is postulated that the effects of oestradiol on peroxidase activity are mediated by uterine epithelial cells but that oestradiol effects on collagen breakdown may be mediated by another cell type.

scholarly journals Ca2+-activated proteinase in the rat. Quantification by immunoassay in the uterus during pregnancy and involution, and in other tissues

1984 ◽  
Vol 220 (2) ◽  
pp. 507-512 ◽  
Author(s):  
J S Elce ◽  
J E Baenziger ◽  
D C Young
Keyword(s):  
Skeletal Muscle ◽  
Small Intestine ◽  
Proteinase Inhibitors ◽  
Post Partum ◽  
Proteinase Activity ◽  
Immunoradiometric Assay ◽  
Protein Blotting ◽  
Uterine Involution ◽  
Wet Weight ◽  
The Mean

Rat uteri were taken at various stages of pregnancy and involution post partum, and several other tissues were taken from pregnant and non-pregnant animals. Portions of each tissue were homogenized in the presence of proteinase inhibitors, and the amounts of the high-Ca2+-requiring Ca2+-activated proteinase in the supernatants were measured by a two-site immunoradiometric assay using 125I-immunoglobulin G. The proteinase was shown, by protein blotting, to be immunologically identical in all tissues. The amounts in the various tissues, expressed in units of proteinase activity/g wet wt., were: lung, 95; kidney and small intestine, 42; liver, 20; brain, heart and skeletal muscle, 13. Uterine wet weight increased at the end of pregnancy by about 8-fold, but the amounts of proteinase per uterus increased by about 22-fold; alternatively, expressed in units of proteinase activity/g wet wt., the mean uterine values were: non-pregnant, 28.6; term-pregnant, 77.0. As the wet weight of the uterus fell rapidly during involution, the amounts of proteinase activity remained relatively high. The data suggest that the Ca2+-activated proteinase may have some role in tissue resorption during uterine involution, but the high proteinase activity present before parturition must be regulated in ways which are not yet clear.

scholarly journals Inhibition by oestrogen of collagen breakdown in the involuting rat uterus

1969 ◽  
Vol 112 (5) ◽  
pp. 637-645 ◽  
Author(s):  
J. F. Woessner
Keyword(s):  
Acid Phosphatase ◽  
Cathepsin D ◽  
Post Partum ◽  
Acid Hydrolase ◽  
Acid Hydrolases ◽  
Rat Uterus ◽  
Oestrogen Treatment ◽  
Period 2 ◽  
Normal Rat ◽  
Wet Weight

1. Both the post-partum involution of the rat uterus and the rapid breakdown of collagen that accompanies it are extensively inhibited by oestrogenic hormones. In the normal rat, 85% of the uterine collagen is degraded within 4 days after parturition; in rats treated with 100μg. of 17β-oestradiol/day, only 35% of uterine collagen is broken down in the same period. 2. Similar effects are produced by diethylstilboestrol if the dose is increased tenfold. 3. Collagen breakdown is inhibited to a greater extent than is the loss of wet weight by oestradiol but not by diethylstilboestrol. 4. The oestrogens appear to act by blocking the breakdown of collagen. There is a greatly decreased concentration of free hydroxyproline in the uterus of treated animals. 5. Acid hydrolase concentrations (β-glucuronidase, β-galactosidase, cathepsin D and acid phosphatase) in the uterus are decreased by oestrogen treatment compared with controls, but the total amounts of these enzymes in the uterus are somewhat elevated. Oestrogens do not appear to inhibit collagen breakdown by altering the concentration and total amount of acid hydrolases.

scholarly journals Oestradiol inhibits collagen breakdown in the involuting rat uterus

1972 ◽  
Vol 127 (4) ◽  
pp. 705-713 ◽  
Author(s):  
Janet N. Ryan ◽  
J. Frederick Woessner
Keyword(s):  
Cathepsin D ◽  
Density Gradient Centrifugation ◽  
Post Partum ◽  
Gradient Centrifugation ◽  
Specific Radioactivity ◽  
Rat Uterus ◽  
Oestradiol Treatment ◽  
Cathepsin D Activity ◽  
Stimulation Of

1. The earlier observation (Woessner, 1969) of oestradiol inhibition of collagen breakdown is confirmed and extended. Administration of 100μg of oestradiol-17β/day to parturient rats strongly inhibits the loss of collagen from the involuting uterus. Three experiments show that this effect is due to an inhibition of collagen degradation rather than to a stimulation of collagen synthesis. 2. Uterine collagen was labelled with hydroxy[14C]-proline by the administration of [14C]proline near the end of pregnancy. By 3 days post partum, control uteri lost 83% of their collagen and 90% of their hydroxy[14C]proline. Uteri from oestradiol-treated rats lost only 50% of both total and labelled hydroxyproline, with no decrease in the specific radioactivity of the hydroxyproline. 3. Incorporation of [14C]proline into uterine collagen hydroxyproline in vivo was not affected by oestradiol treatment. 4. Urinary excretion of hydroxyproline was increased in post-partum control rats and decreased in oestradiol-treated rats. 5. An enzyme capable of cleaving 4-phenylazobenzyloxycarbonyl-l-prolyl-l-leucylglycyl- l-prolyl-d-arginine (a substrate for clostridial collagenase) increased in activity in the post-partum uterus and was unaffected by oestradiol treatment. 6. Uterine homogenates digested uterine collagen extensively at pH3.2. This digestion was unaffected by the oestradiol treatment. 7. Lysosomal fractions prepared by density-gradient centrifugation of uterine homogenates contained coincident peaks of cathepsin D activity and peptide-bound hydroxyproline. The cathepsin D and hydroxyproline contents of this peak were unaffected by oestradiol treatment.

GLYCOGEN IN THE DECIDUAL TISSUE OF THE RAT UTERUS

1962 ◽  
Vol 25 (1) ◽  
pp. 69-76 ◽  
Author(s):  
H. C. CECIL ◽  
J. BITMAN ◽  
M. R. CONNOLLY ◽  
T. R. WRENN
Keyword(s):  
Glycogen Content ◽  
Endometrial Tissue ◽  
Rat Uterus ◽  
Glycogen Concentration ◽  
Decidual Tissue ◽  
Wet Weight ◽  
Glycogen Deposition

SUMMARY Glycogen was investigated in uteri of intact and progesterone-treated spayed rats with and without deciduomata. Samples of whole uterus, endometrium and myometrium were analysed. With development of deciduoma in intact animals the glycogen concentration of whole uterus increased from 68 to 125 mg./100 g. wet weight. There was no change in the myometrial glycogen concentration; i.e. 74 mg./100 g. without deciduoma and 73 mg./100 g. wet weight in the decidual myometrium. The endometrial glycogen content of decidual tissue was 221 mg./100 g. wet weight. Since myometrial glycogen was constant, the increases observed in the decidual tissue of whole uteri must be due to an increase in the amount of endometrium and/or an increase in the concentration of glycogen in the endometrium. As the deciduoma developed the proportion of endometrium increased from 9% in the uninjured horn to 34% in the injured horn. Thus, an increase in the amount of endometrium contributes to the increase in the glycogen concentration. Similar changes were observed in whole uterus, myometrium and endometrium of the spayed animals treated with progesterone. Previous work on uterine glycogen in rats indicated that oestrogens cause glycogen deposition and this occurs only in the myometrium, while progesterone exhibits no effect. The present results demonstrate that progesterone is responsible for the glycogen increase by stimulating the growth of endometrium—a glycogen-rich tissue. Since no endometrial tissue could be obtained from horns without decidual development, this study could not determine whether progesterone had any effect on glycogen deposition.

Macroscopic uterine involution in the post-partum Boer goat

1991 ◽  
Vol 4 (3) ◽  
pp. 277-283 ◽  
Author(s):  
J.P.C. Greyling ◽  
C.H. van Niekerk
Keyword(s):  
Post Partum ◽  
Boer Goat ◽  
Uterine Involution

scholarly journals Clinical, Ultrasonographic, Bacteriological, Cytological and Histopathological Findings of Uterine Involution in Ewes with Uterine Infection

Pathogens ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 54 ◽  
Author(s):  
Katerina S. Ioannidi ◽  
Natalia G. C. Vasileiou ◽  
Marianna S. Barbagianni ◽  
Denise C. Orfanou ◽  
George Mantziaras ◽  
...  
Keyword(s):  
Reproductive Performance ◽  
Post Partum ◽  
Vaginal Swab ◽  
Post Inoculation ◽  
Tissue Samples ◽  
E Coli ◽  
Uterine Infection ◽  
Uterine Involution ◽  
Group I ◽  
Significant Difference

The objectives of the study were (a) to study the characteristics of uterine involution in ewes that had developed subclinical uterine infection in the immediately post-partum period and (b) to evaluate effects of the infection in the subsequent reproductive performance of ewes. Uterine infection was induced in ewes (I, n = 10) by intrauterine inoculation of Escherichia coli; uninoculated controls were included (C, n = 12). Animals were examined at regular intervals before and post-inoculation. Clinical and ultrasonographic examinations were performed. Vaginal swab samples and biopsy uterine tissue samples were collected for bacteriological, cytological and histological examination. Finally, ewes were put to rams and reproductive performance was monitored. After challenge, it was ultrasonographically found that caruncular dimensions, myometrial thickness and diameter of uterine lumen were greater in I ewes. In these ewes, particular reduction of dimensions occurred during the second week post-partum, whilst in C ewes during the first week. The uterine artery diameter and the blood flow into the uterus were also greater in I than in C ewes. E. coli infection was more frequent and of longer duration in I than in C ewes: in 68.1% and 50.0% of ewes and 19.5 and 14 days, respectively. There was lower proportion of neutrophils and higher of lymphocytes in group I than in C. In inoculated ewes, there was histological evidence of uterine epithelial destruction, increased cellular infiltration, hyperaemia and extracasation, which persisted up to 42 days post-partum. During the subsequent reproductive season, all ewes in group I lambed normally and produced healthy and viable lambs. No significant difference in reproductive performance parameters were seen in I comparison to C ewes. It is concluded that the innate immunity of the uterus sufficed to counteract the bacterial infection, although the process of involution took longer than in healthy animals; moreover, the ultrasonographic examination is a useful means for assessment of the genital tract of ewes post-partum; finally, no adverse effects were noted in the subsequent reproductive performance of ewes.

A note on the effect of suckling stimulus on uterine involution, post-partum ovarian activity and fertility in Nili-Ravi buffaloes

1985 ◽  
Vol 41 (1) ◽  
pp. 119-122 ◽  
Author(s):  
R. H. Usmani ◽  
N. Ullah ◽  
S. K. Shah
Keyword(s):  
Post Partum ◽  
Follicular Development ◽  
Reproductive Organs ◽  
Ovarian Activity ◽  
Corpora Lutea ◽  
Limited Data ◽  
Uterine Involution ◽  
Negative Effect ◽  
The Difference ◽  
Larger Sample

ABSTRACTNineteen pluriparous buffaloes of Nili-Ravi breed which calved during the months of October and November 1983 were studied for the effects of sucking stimulus on the uterine involution, post-partum ovarian functions and fertility. On the day of calving, buffaloes were assigned to either a limited-suckling (LS) or non-suckling (NS) group. Changes in reproductive organs were monitored by rectal palpations at weekly intervals. Buffaloes were observed for oestrus twice daily (04.00 and 18.00 h) with the help of a teaser bull, and were artificially inseminated at the first post-partum and each subsequent oestrus. LS buffaloes had a shorter period to uterine involution (20 days) than NS buffaloes (28 days). Intervals to regression of the corpora lutea of pregnancy and to resumption of post-partum follicular development did not differ in the two groups. LS buffaloes had longer intervals to first post-partum oestrus and conception (54 and 88 days respectively) than NS buffaloes (39 and 68 days respectively). However, the difference in services per conception of LS and NS buffaloes was non-significant (2-05 v. 1·62). These limited data reveal that the suckling stimulus has a negative effect on the post-partum resumption of oestrous activity, and that conception is delayed. Further studies are indicated to verify these observations in a larger sample size and during all seasons of the year.

Sign in / Sign up

Export Citation Format

Share Document