scholarly journals Expression of fibroblast growth factors and their receptors during full-thickness skin wound healing in young and aged mice

2005 ◽  
Vol 186 (2) ◽  
pp. 273-289 ◽  
Author(s):  
Akiko Komi-Kuramochi ◽  
Mitsuko Kawano ◽  
Yuko Oda ◽  
Masahiro Asada ◽  
Masashi Suzuki ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Full Thickness ◽  
Healthy Skin ◽  
Aged Mice ◽  
Full Thickness Skin ◽  
Thickness Skin

The highly ordered process of wound healing involves the coordinated regulation of cell proliferation and migration and tissue remodeling, predominantly by polypeptide growth factors. Consequently, the slowing of wound healing that occurs in the aged may be related to changes in the activity of these various regulatory factors. To gain additional insight into these issues, we quantified the absolute copy numbers of mRNAs encoding all the fibroblast growth factors (FGFs), their receptors (FGFRs) and two other growth factors in the dorsal skin of young and aged mice during the healing of full-thickness skin excisional wounds. In young adult mice (8 weeks old), FGF7, FGF10 and FGF22 mRNAs were all strongly expressed in healthy skin, and levels of FGF7 and 10 but not 22 increased 2- to 3.5-fold over differing time courses after wounding. The levels of FGF9, 16, 18 and especially 23 mRNAs were moderate or low in healthy skin but increased 2- to 33-fold after wounding. Among the four FGFRs, expression of only FGFR1 mRNA was augmented during wound healing. Expression of transforming growth factor-β and hepatocyte growth factor was also high in healthy skin and was upregulated during healing. Notably, in aged mice (35 weeks old), where healing proceeded more slowly than in the young, both the basal and wound-induced mRNA expression of most of these genes was reduced. While these results confirm the established notion that FGFR2 IIIB ligands (FGF7 and FGF10) are important for wound healing, they also suggest that decreased expression of multiple FGF ligands contributes to the slowing of wound healing in aged mice and indicate the potential importance of further study of the involvement of FGF9, 16, 18 and 23 in the wound healing process.

scholarly journals Full-thickness skin reconstruction with basic fibroblast growth factor-impregnated collagen-gelatin sponge

2019 ◽  
Vol 11 ◽  
pp. 81-87 ◽  
Author(s):  
Hajime Matsumine ◽  
Hiroshi Fujimaki ◽  
Mika Takagi ◽  
Satoko Mori ◽  
Tomohiro Iwata ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Gelatin Sponge ◽  
Fibroblast Growth ◽  
Full Thickness ◽  
Full Thickness Skin ◽  
Thickness Skin ◽  
Skin Reconstruction

scholarly journals The Antimicrobial Peptide Human β-Defensin-3 Accelerates Wound Healing by Promoting Angiogenesis, Cell Migration, and Proliferation Through the FGFR/JAK2/STAT3 Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Miho Takahashi ◽  
Yoshie Umehara ◽  
Hainan Yue ◽  
Juan Valentin Trujillo-Paez ◽  
Ge Peng ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Growth Factors ◽  
Growth Factor ◽  
Antimicrobial Peptide ◽  
Fibroblast Growth Factor ◽  
Human Fibroblasts ◽  
Janus Kinase ◽  
Fibroblast Growth ◽  
Angiogenic Growth Factors

In addition to its antimicrobial activity, the skin-derived antimicrobial peptide human β-defensin-3 (hBD-3) promotes keratinocyte proliferation and migration to initiate the wound healing process; however, its effects on fibroblasts, which are the major cell type responsible for wound healing, remain unclear. We investigated the role of hBD-3 in cell migration, proliferation and production of angiogenic growth factors in human fibroblasts and evaluated the in vivo effect of hBD-3 on promoting wound healing and angiogenesis. Following hBD-3 treatment, the mouse wounds healed faster and showed accumulation of neutrophils and macrophages in the early phase of wound healing and reduction of these phagocytes 4 days later. hBD-3-treated wounds also displayed an increased number of fibroblasts and newly formed vessels compared to those of the control mice. Furthermore, the expression of various angiogenic growth factors was increased in the hBD-3-treated wounds. Additionally, in vitro studies demonstrated that hBD-3 enhanced the secretion of angiogenic growth factors such as fibroblast growth factor, platelet-derived growth factor and vascular endothelial growth factor and induced the migration and proliferation of human fibroblasts. The hBD-3-mediated activation of fibroblasts involves the fibroblast growth factor receptor 1 (FGFR1)/Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathways, as evidenced by the inhibitory effects of pathway-specific inhibitors. We indeed confirmed that hBD-3 enhanced the phosphorylation of FGFR1, JAK2 and STAT3. Collectively, the current study provides novel evidence that hBD-3 might be a potential candidate for the treatment of wounds through its ability to promote wound healing, angiogenesis and fibroblast activation.

scholarly journals Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

2018 ◽  
Vol 27 (10) ◽  
pp. 1535-1547 ◽  
Author(s):  
Niann-Tzyy Dai ◽  
Wen-Shyan Huang ◽  
Fang-Wei Chang ◽  
Lin-Gwei Wei ◽  
Tai-Chun Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Plasma ◽  
Three Dimensional ◽  
Skin Substitute ◽  
Strength Analysis ◽  
Full Thickness ◽  
Skin Substitutes ◽  
Full Thickness Skin ◽  
Post Surgery ◽  
Thickness Skin

Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay. Experimental studies in mouse full-thickness skin wound models showed that the pre-vascularized gel scaffold significantly accelerated wound healing 7 days after surgery, and resembled normal skin structures after 14 days post-surgery. Histological analysis revealed that pre-vascularized gel scaffolds were well integrated in the host skin, resulting in the vascularization of both the epidermis and dermis in the wound area. Moreover, mechanical strength analysis demonstrated that the healed wound following the implantation of the pre-vascularized gel scaffolds exhibited good tensile strength. Taken together, this novel pre-vascularized human plasma gel scaffold has great potential in skin tissue engineering.

Cytokines and Growth Factors Involved in Peritoneal Fibrosis of Peritoneal Dialysis Patients

2005 ◽  
Vol 28 (2) ◽  
pp. 129-134 ◽  
Author(s):  
K.-H. Oh ◽  
P.J. Margetts
Keyword(s):  
Peritoneal Dialysis ◽  
Growth Factors ◽  
Growth Factor ◽  
Transforming Growth Factor ◽  
Fibroblast Growth Factors ◽  
Tissue Growth ◽  
Platelet Derived Growth Factor ◽  
Fibroblast Growth ◽  
Dialysis Patients ◽  
Peritoneal Fibrosis

Peritoneal fibrosis is initiated by exposure of peritoneal tissues to numerous harmful agents encountered during peritoneal dialysis. These agents interact with cells within the peritoneum to induce growth factors and cytokines that are important in the initiation, progression and maintenance of fibrosis. Some of the mediators implicated in the pathogenesis of peritoneal fibrosis include transforming growth factor (TGF) ß, connective tissue growth factor (CTGF), fibroblast growth factors (FGF), and platelet derived growth factor (PDGF).

Differential Effects of Fibroblast Growth Factors on Expression of Genes of the Plasminogen Activator and Insulin-like Growth Factor Systems by Human Breast Fibroblasts

2002 ◽  
Vol 87 (04) ◽  
pp. 674-683 ◽  
Author(s):  
John Martens ◽  
Lambert Dorssers ◽  
Jan Klijn ◽  
John Foekens ◽  
Anieta Sieuwerts
Keyword(s):  
Protein Synthesis ◽  
Growth Factors ◽  
Growth Factor ◽  
Plasminogen Activator ◽  
De Novo ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Human Breast ◽  
Short Term ◽  
De Novo Protein Synthesis

SummaryIn breast stroma urokinase plasminogen activator (uPA) is predominantly expressed by fibroblasts located in the near vicinity of tumor cells, and fibroblast-derived insulin-like growth factor-1 (IGF-1) may be involved in inhibiting the expression of uPA in these fibroblasts. To investigate a possible role for fibroblast growth factors (FGFs), we evaluated the expression of components of the PA system and the IGF system in normal and tumor-tissue-derived human breast fibroblasts exposed to various FGFs in vitro. mRNA analysis revealed that FGF-1, FGF-2 and FGF-4 induced the mRNA expression levels of uPA, tPA, uPAR, PAI-1 and PAI-2, and reduced those of IGF-1, IGF-1R, IGF-2R and IGFBP-4, without significantly affecting the levels of IGFBP-3, IGFBP-5 and IGFBP-6 mRNA. Concerning the expression of IGF-2 mRNA, the effects mediated by FGF-1, FGF-2 and FGF-4 were divergent. In general, the effects elicited by FGF-1 on the various mRNA levels studied were rapid and short-term. Those mediated by FGF-2 overall lagged behind but were longer-lasting. For FGF-4 an in between pattern was observed. Blocking transcription and translation demonstrated that a) both the FGF-1 and FGF-2 induced effects were the result of altered gene transcription or mRNA stability, b) the short-term effects mediated by FGF-1 and FGF-2 required de novo protein synthesis, and c) the long-term effects elicited by FGF-2 did not depend on de novo protein synthesis during the first 24 h, but were triggered by proteins produced or made available thereafter. The data presented propose that of the FGFs studied (FGF-1, -2, -4, -5, and -7), FGF-2 is the most attractive target for therapeutical strategies aimed at diminishing the contribution of stromal fibroblasts in the PA-directed breast tumor proteolysis.

scholarly journals Pharmaceutical application of frog skin on full-thickness skin wound healing in mice

2013 ◽  
Vol 51 (12) ◽  
pp. 1600-1606 ◽  
Author(s):  
Mahere Rezazade Bazaz ◽  
Mohammad Mashreghi ◽  
Nasser Mahdavi Shahri ◽  
Mansour Mashreghi ◽  
Ahmad Asoodeh ◽  
...  
Keyword(s):  
Wound Healing ◽  
Frog Skin ◽  
Skin Wound ◽  
Full Thickness ◽  
Skin Wound Healing ◽  
Full Thickness Skin ◽  
Pharmaceutical Application ◽  
Thickness Skin
Sign in / Sign up

Export Citation Format

Share Document