scholarly journals Prevalence and Concentration of Escherichia coli O157:H7 in Cattle, Products, and the Environment in the United States of America: A Meta-Analysis Study

2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Woube Y ◽  
Abdella E ◽  
Faraj R ◽  
Perry R ◽  
Reddy G ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Dairy Cattle ◽  
United States Of America ◽  
Meta Analysis ◽  
The United States ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Pooled Prevalence ◽  
Significant Difference

Shiga toxin-producing Escherichia coli O157:H7 are bacterial pathogens that cause foodborne infections in humans. The objectives of this study were to find the pooled prevalence and concentration of Escherichia coli O157:H7 in cattle, hides, carcass, and the environment in the United States of America using meta-analysis. The PRISMA and MOOSE research protocols were employed in the methodology. Weighted effect size was calculated using MetaXL software. A total of 1737 publications were screened, out of which 53 were selected for the final analysis. The pooled prevalence in feedlot cattle was 10.96% (95% CI: 4.2-18.8%). In dairy cattle a pooled prevalence of 1.5% (95% CI: 0.11-3.5%) was observed. The prevalence between feedlot and dairy cattle was significantly different (p<0.05). The herd prevalence in combined feedlot and dairy cattle was 31.7% (95% CI: 10.2-55.5%). Hide and carcass samples’ pooled prevalences were 54.7% (95% CI: 41.7-67.5%) and 21.3% (95% CI: 9.7-34.2%), respectively. Prevalence of environmental samples was 8.1% for produce (95% CI: 0-29.6%), 4.6% for watershed and sediment samples (95% CI: 0-12.2%), and 2.4% for water taken from troughs (95% CI: 0.39-5.1%). Significant difference was observed in individual, herd, and environment prevalence between regions (χ2 =903.14, p=0.0000; χ2 =11.06, p=0.0039; χ2 =13.59, p=0.0004, respectively). E. coli O157:H7 concentrations were highest in feces (900- 300,000 cfu/g), followed by hides (5-9,800 cfu/100 square cm), and carcass (1-189 cfu/100 square cm). At least one supershedder exists in a herd. The findings in this study showed that Escherichia coli O157:H7 serotype is widespread in feedlots, herds, hides, and carcass in the United States of America necessitating appropriate measures to prevent human illnesses. Improving management programs in cattle herds, reduction of environmental contamination, and hygienic slaughter practices are targets of intervention.

Fecal Shedding of Escherichia coli O157:H7 in North Dakota Feedlot Cattle in the Fall and Spring

2006 ◽  
Vol 69 (5) ◽  
pp. 1154-1158 ◽  
Author(s):  
MARGARET L. KHAITSA ◽  
MARC L. BAUER ◽  
GREGORY P. LARDY ◽  
DAWN K. DOETKOTT ◽  
REDEMPTA B. KEGODE ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
North Dakota ◽  
The United States ◽  
Extreme Weather ◽  
Early Spring ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
E Coli

Cattle are an important reservoir of Escherichia coli O157:H7, which can lead to contamination of food and water, and subsequent human disease. E. coli O157:H7 shedding in cattle has been reported as seasonal, with more animals shedding during summer and early fall than during winter. North Dakota has relatively cold weather, especially in winter and early spring, compared with many other regions of the United States. The objective was to assess fecal shedding of E. coli O157: H7 in North Dakota feedlot cattle over the fall, winter, and early spring. One hundred forty-four steers were assigned randomly to 24 pens on arrival at the feedlot. Samples of rectal feces were obtained from each steer four times (October and November 2003, and March and April 2004) during finishing. On arrival (October 2003), 2 (1.4%) of 144 cattle were shedding E. coli O157:H7. The shedding increased significantly to 10 (6.9%) of 144 after 28 days (November 2003), to 76 (53%) of 143 at the third sampling (March 2004), and dropped significantly to 30 (21%) of 143 at the fourth (last) sampling (March 2004) before slaughter. Unfortunately, we were unable to sample the cattle during winter because of the extreme weather conditions. Sampling time significantly (P &lt; 0.0001) influenced variability in E. coli O157:H7 shedding, whereas herd (P = 0.08) did not. The prevalence of E. coli O157:H7 shedding in North Dakota steers in fall and early spring was comparable to what has been reported in other parts of the United States with relatively warmer weather. Further research into E. coli O157:H7 shedding patterns during extreme weather such as North Dakota winters is warranted in order to fully assess the seasonal effect on the risk level of this organism.

Epidemiology of Escherichia coli O157 in Feedlot Cattle

1997 ◽  
Vol 60 (5) ◽  
pp. 462-465 ◽  
Author(s):  
DALE D. HANCOCK ◽  
DANIEL H. RICE ◽  
LEE ANN THOMAS ◽  
DAVID A. DARGATZ ◽  
THOMAS E. BESSER
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
The United States ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Samples ◽  
E Coli ◽  
Geographical Regions ◽  
Flagellar Antigen ◽  
Low Prevalence

Fecal samples from cattle in 100 feedlots in 13 states were bacteriologically cultured for Escherichia coli O157 that did not ferment sorbitol, lacked beta-glucuronidase, and possessed genes coding for Shiga-like toxin. In each feedlot 30 fresh fecal-pat samples were collected from each of four pens: with the cattle shortest on feed, with cattle longest on feed, and with cattle in two randomly selected pens. E. coli O157 was isolated from 210 (1.8%) of 11,881 fecal samples. One or more samples were positive for E. coli O157 in 63 of the 100 feedlots tested. E. coli O157 was found at roughly equal prevalence in all the geographical regions sampled. The prevalence of E. coli O157 in the pens with cattle shortest on feed was approximately threefold higher than for randomly selected and longest on feed pens. Of the E. coli O157 isolates found in this study, 89.52% expressed the H7 flagellar antigen. E. coli O157 was found to be widely distributed among feedlot cattle, but at a low prevalence, in the United States.

Escherichia coli O157:H7 Lineages in Healthy Beef and Dairy Cattle and Clinical Human Cases in Alberta, Canada

2009 ◽  
Vol 72 (3) ◽  
pp. 601-607 ◽  
Author(s):  
RANJANA SHARMA ◽  
KIM STANFORD ◽  
MARIE LOUIE ◽  
KRYSTY MUNNS ◽  
S. JACOB JOHN ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Tetracycline Resistance ◽  
Geographic Location ◽  
Feedlot Cattle ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Strain Diversity ◽  
Significant Difference ◽  
Southern Alberta ◽  
Northern Alberta

The aim of this study was to examine the prevalence and distribution of Escherichia coli O157:H7 lineage-specific polymorphism assay (LSPA) 6 genotypes from cattle (n = 313) and clinical human (n = 203) isolates from northern and southern Alberta, Canada, to understand possible associations of genotypes with host and geographic location. The majority of cattle isolates (feedlot and dairy) typed as LSPA-6 111111 (72.2%), with proportionately higher LSPA-6 222222 (19.4%) than other LSPA-6 genotypes (10.7%). Clinical human isolates also typed primarily as LSPA-6 111111 (90.1%), but a higher percentage of genotypes (6.8%) other than LSPA-6 222222 (3.1%) was observed. A significantly higher frequency of LSPA-6 111111 in southern Alberta cattle (P &lt; 0.0001) and a significant difference in LSPA-6 genotypes between human versus feedlot cattle from northern Alberta (P &lt; 0.0001) were detected. LSPA-6 211111 genotype was third and second most common in cattle and humans, respectively, and several new LSPA-6 genotypes (n = 19) were also discovered. Despite avoiding over-representation of isolates from specific farms or outbreaks, higher strain diversity among cattle by pulsed-field gel electrophoresis (PFGE; 50 genotypes) in contrast to human (9 PFGE genotypes) isolates was observed. The majority of cattle (74.4%) and human (90.6%) isolates were susceptible to the antimicrobials tested. Within resistant cattle isolates, sulfisoxazole-tetracycline resistance was common (62.5%) and was accounted for by the presence of sul1 and sul2, and tet(A) and tet(B) determinants. An association between LSPA-6 and PFGE genotypes but not between geographic location and PFGE genotype for both hosts was evident.

scholarly journals Comparison of the Reveal 20-Hour Method and the BAM Culture Method for the Detection of Escherichia coli O157:H7 in Selected Foods and Environmental Swabs: Collaborative Study

2001 ◽  
Vol 84 (3) ◽  
pp. 737-751 ◽  
Author(s):  
Charles B Bird ◽  
Rebecca J Hoerner ◽  
Lawrence Restaino ◽  
G Anderson ◽  
W Birbari ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Collaborative Study ◽  
Culture Method ◽  
The United States ◽  
Escherichia Coli O157 ◽  
Private Industry ◽  
E Coli ◽  
Different Levels

Abstract Four different food types along with environmental swabs were analyzed by the Reveal for E. coli O157:H7 test (Reveal) and the Bacteriological Analytical Manual (BAM) culture method for the presence of Escherichia coli O157:H7. Twenty-seven laboratories representing academia and private industry in the United States and Canada participated. Sample types were inoculated with E. coli O157:H7 at 2 different levels. Of the 1095 samples and controls analyzed and confirmed, 459 were positive and 557 were negative by both methods. No statistical differences (p &lt;0.05) were observed between the Reveal and BAM methods.

How Does Escherichia coli O157:H7 Testing in Meat Compare with What We Are Seeing Clinically?

2000 ◽  
Vol 63 (6) ◽  
pp. 819-821 ◽  
Author(s):  
DAVID W. K. ACHESON
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Shiga Toxin ◽  
Food Supply ◽  
The United States ◽  
Escherichia Coli O157 ◽  
Current Policy ◽  
E Coli ◽  
Different Types ◽  
Uremic Syndrome

Escherichia coli O157:H7 is but one of a group of Shiga toxin-producing E. coli (STEC) that cause both intestinal disease such as bloody and nonbloody diarrhea and serious complications like hemolytic uremic syndrome (HUS). While E. coli O157: H7 is the most renowned STEC, over 200 different types of STEC have been documented in meat and animals, at least 60 of which have been linked with human disease. A number of studies have suggested that non-O157 STEC are associated with clinical disease, and non-O157 STEC are present in the food supply. Non-O157 STEC, such as O111 have caused large outbreaks and HUS in the United States and other countries. The current policy in the United States is to examine ground beef for O157:H7 only, but restricting the focus to O157 will miss other important human STEC pathogens.

P760Clinical profile of African-American and non-Hispanic caucasian patients with Takotsubo cardiomyopathy syndrome in a large urban hospital in the United States of America

2019 ◽  
Vol 40 (Supplement_1) ◽  
Author(s):  
P Westman ◽  
K Ravindra ◽  
J Chiabrando ◽  
D Kadariya ◽  
G Maehara ◽  
...  
Keyword(s):  
United States ◽  
African American ◽  
Hospital Mortality ◽  
United States Of America ◽  
Troponin I ◽  
The United States ◽  
Stress Cardiomyopathy ◽  
Urban Hospital ◽  
Significant Difference ◽  
Caucasian Patients

Abstract Background Takotsubo (stress) cardiomyopathy is an acute reversible heart failure syndrome initially described in Japanese patients, but now well characterized in Caucasians patients in Europe or of European descent. An initial observation has suggested a lower incidence of Takotsubo in non-Caucasian subjects, particularly in the African-American (AA) population in the United States of America. The purpose of this study was to assess whether epidemiologic and clinical differences were present in Takotsubo in a large urban hospital in Virginia, USA. Methods We used an informatics-based system to query electronic health records (TriNetX, Cambridge, MA, USA) to search for cases of Takotsubo between 2010 and 2018 and a corresponding cohort of patients with non-ST segment elevation acute myocardial infarction (NSTEMI). We then performed a chart-level review of 160 cases and obtained additional clinical information including symptoms, risk factors, co-morbidities, and in-hospital outcomes. This retrospective study was approved by the Institutional Review Board of our institution. Results We identified 260 cases of Takotsubo and 6,270 of NSTEMI in the same time period (1:24, 4.2%). Being AA was associated with an odds ratio of Takotsubo versus NSTEMI of 0.38 [0.29–0.50] (P=0.0001). With further evaluation of patients with Takotsubo (N=160), AA (N=44, 27.2%) and Non-Hispanic Caucasian (C) (N=110, 67.9%) had no differences in age and sex. AA patients with Takotsubo however were more likely than C patients to be affected by type II diabetes mellitus (38.6% versus 14.5%, P=0.002, OR 3.70 [1.65–8.28]), have history of drug abuse (27.3% versus 9.1%, P=0.009, OR 3.75 [1.48–9.49]) and of cocaine use in particular (9.1% versus 0.9%, P=0.024, OR 11.0 [1.19–101.4]). The pattern of wall motion abnormality was not different between the 2 groups. AA patients presented with a lower ratio of brain natriuretic peptide (BNP) to troponin I (41.9 [12.7–258] pg./ml versus 281 [42–890] pg/ml, P=0.022). There was no significant difference of in-hospital mortality between the AA and C groups (9.1% versus 25%, respectively, OR 0.40 [0.13–1.24], P=0.11). Conclusions The incidence and clinical characteristics of Takotsubo (stress) cardiomyopathy appear to be different between African-American and Non-Hispanic Caucasian patients. African-American patients are more likely to have diabetes and illicit drug usage, but have a lower BNP/troponin I ratio. Both AA and Non-Hispanic Caucasian patients have similar in-hospital mortality.

Economic Cost of Illness Due to Escherichia coli O157 Infections in the United States

2005 ◽  
Vol 68 (12) ◽  
pp. 2623-2630 ◽  
Author(s):  
PAUL D. FRENZEN ◽  
ALISON DRAKE ◽  
FREDERICK J. ANGULO ◽  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Medical Care ◽  
Cost Of Illness ◽  
Severity Of Illness ◽  
Economic Cost ◽  
The United States ◽  
Escherichia Coli O157 ◽  
Emerging Infections ◽  
Surveillance Network

The Centers for Disease Control and Prevention (CDC) has estimated that Shiga toxin–producing Escherichia coli O157 (O157 STEC) infections cause 73,000 illnesses annually in the United States, resulting in more than 2,000 hospitalizations and 60 deaths. In this study, the economic cost of illness due to O157 STEC infections transmitted by food or other means was estimated based on the CDC estimate of annual cases and newly available data from the Foodborne Diseases Active Surveillance Network (FoodNet) of the CDC Emerging Infections Program. The annual cost of illness due to O157 STEC was $405 million (in 2003 dollars), including $370 million for premature deaths, $30 million for medical care, and $5 million in lost productivity. The average cost per case varied greatly by severity of illness, ranging from $26 for an individual who did not obtain medical care to $6.2 million for a patient who died from hemolytic uremic syndrome. The high cost of illness due to O157 STEC infections suggests that additional efforts to control this pathogen might be warranted.

Evaluation of a Direct-Fed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle†

2010 ◽  
Vol 73 (2) ◽  
pp. 366-371 ◽  
Author(s):  
TERRANCE M. ARTHUR ◽  
JOSEPH M. BOSILEVAC ◽  
NORASAK KALCHAYANAND ◽  
JAMES E. WELLS ◽  
STEVEN D. SHACKELFORD ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intervention Strategy ◽  
Feedlot Cattle ◽  
Subtilis Strain ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Microbial Product ◽  
Significant Difference ◽  
Antimicrobial Intervention ◽  
And Control

Direct-fed microbials (DFM) have been identified as potential preharvest interventions for the reduction of foodborne bacterial pathogens such as Escherichia coli O157:H7. This study evaluated the efficacy of a DFM consisting of Bacillus subtilis strain 166 as an antimicrobial intervention strategy for the reduction of prevalence and load of E. coli O157:H7 in feces and on hides of feedlot cattle. Cattle (n = 526) were divided among 16 feedlot pens. Half of the pens received the DFM, and the other half did not. Hide and fecal samples were collected from each animal on days 28, 63, and 84 of the feeding trial. Over the course of the 84-day feeding period, there were no significant differences observed between treatments for either hide or fecal prevalence of E. coli O157:H7, or for the percentage of animals that were shedding E. coli O157:H7 at high levels (≥200 CFU/g) in their feces or harboring E. coli O157:H7 at high levels (≥40 CFU/cm2) on their hides. In addition, there was no significant difference between the average daily gains for the treated and control groups, with both groups averaging 1.3 kg/day. We concluded that the DFM tested would not be an effective preharvest intervention against E. coli O157:H7.

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