scholarly journals IMMOBILIZATION OF CELLS OF NITRILE-HYDROLYZING BACTERIA RHODOCOCCUS ERYTHROPOLIS 4-1 AND ALCALIGENES FAECALIS 2 USING THERMOTROPIC AND IONOTROPIC GELS

2020 ◽  
pp. 26-32
Author(s):  
E. M. Mochalova ◽  
◽  
Yu. G. Maksimova ◽  
◽  
Keyword(s):  
Acrylic Acid ◽  
Bacterial Cell ◽  
Rhodococcus Erythropolis ◽  
Cell Immobilization ◽  
Alcaligenes Faecalis ◽  
Operational Stability ◽  
Stable Operation ◽  
Bacterial Cell Immobilization

Cells of amidase containing bacteria of Rhodococcus erythropolis 4-1 and Alcaligenes faecalis 2 were immobilized using carriers such as barium alginate, agarose, chitosan and κ-carrageenan. The aim of the work was to study the effect of bacterial cell immobilization methods on the operational stability of a bio-catalyst. It has been determined that the best carrier for the stable operation of the biocatalyst was aga-rose. The highest amount of acrylic acid was obtained during multi-cycle transformation of acrylamide by immobilized A. faecalis 2 cells.

scholarly journals Immobilization of Aeromonas bivalvium PT2 Cells with Alginate and Measurement of Chitinolytic Activities

2020 ◽  
Vol 147 ◽  
pp. 03020
Author(s):  
Dita P. Saputri ◽  
Ustadi
Keyword(s):  
Bacterial Cell ◽  
Cell Immobilization ◽  
Chitinase Activity ◽  
Nutrient Broth ◽  
Chitinolytic Activity ◽  
Immobilized Bacteria ◽  
Chitinolytic Bacteria ◽  
Bacterial Immobilization ◽  
The Stability ◽  
Bacterial Cell Immobilization

Aeromonas bivalvium is one of the chitinolytic bacteria that able to degrade chitin into its derivatives. These bacteria can only be used once during the fermentation process, which is less profitable to be applied in industrial scale. This limitation can be solved by bacterial immobilization method. This study aimed to determine the effect of bacterial cell immobilization on chitinolytic activity and to determine the stability of the immobilized bacteria during repeated usage. Bacterial cell immobilization was carried out by entrapment method with 1% sodium alginate matrix. Immobilized bacteria was cultured in two different mediums, namely nutrient broth (NB) and nutrient broth (NB) added with colloidal chitin (NB + K). Tests for chitinolytic activity were carried out in bacteria. In addition, the stability of immobilized bacteria was also tested for chitinolytic activity with repeated removal and use. The result shows that the effectiveness of immobilization on average is 91.8%. Immobilization did not significantly affect chitinolytic activity when compared with bacteria without immobilization. Immobilized bacteria in this study has similar performance as bacteria without immobilization. The results of the stability tests including chitinase activity and NAG released indicated a significant decline during repeated usage with maximum usage of three times.

Removal of environmental estrogens by bacterial cell immobilization technique

Chemosphere ◽  
2016 ◽  
Vol 144 ◽  
pp. 607-614 ◽  
Author(s):  
Cong Ma ◽  
Dan Qin ◽  
Qian Sun ◽  
Fangfang Zhang ◽  
Heqin Liu ◽  
...  
Keyword(s):  
Bacterial Cell ◽  
Cell Immobilization ◽  
Environmental Estrogens ◽  
Bacterial Cell Immobilization

Biocatalysts Based on Bacterial Strain Cells with Amidase Activity for Synthesis of Acrylic Acid from Acrylamide

2019 ◽  
Vol 19 (1) ◽  
pp. 73-79
Author(s):  
Yu. G. Maksimova ◽  
M. S. Yakimova ◽  
A. Yu. Maksimov
Keyword(s):  
Acrylic Acid ◽  
Bacterial Strain ◽  
Rhodococcus Erythropolis ◽  
Alcaligenes Faecalis ◽  
Optimal Concentration ◽  
Optimal Temperature ◽  
Amidase Activity ◽  
Glutaric Aldehyde ◽  
Optimal Ph ◽  
Biocatalytic Process

A biocatalytic process for synthesis of acrylic acid was studied in the presence of Rhodococcus erythropolis 4-1 and Alcaligenes faecalis 2 strains with the pronounced amidase activity. The optimal pH of the process was 6–7 for R. erythropolis 4-1 and 7–7.5 for A. faecalis 2, optimal temperature 20–50 °C for both strains, optimal concentration of acrylamide 150 mM for R. erythropolis 4-1 and 250 mM for A. faecalis 2. At the stepwise addition of the substrate, the synthesis was more effective with A. faecalis 2 than with R. erythropolis 4-1. Freezing at –20 °C was shown preferable for storing the biocatalysts. The amidase activity of both humid and dry stored A. faecalis 2 cells immobilized on activated glutaric aldehyde and non-activated chitosan was not decreased.

Bacterial Decolorization of Acid Orange 7 in the Presence of Ionic and Non-Ionic Surfactants

2007 ◽  
Vol 62 (1-2) ◽  
pp. 87-92 ◽  
Author(s):  
Tatyana Avramova ◽  
Lilyana Stefanova ◽  
Blaga Angelova ◽  
Sava Mutafov
Keyword(s):  
Cationic Surfactant ◽  
Anionic Surfactant ◽  
Rhodococcus Erythropolis ◽  
Reaction Medium ◽  
Alcaligenes Faecalis ◽  
Ionic Surfactant ◽  
Acid Orange 7 ◽  
Prolonged Incubation ◽  
Acid Orange ◽  
Triton X

The effects of the non-ionic surfactant Triton® X-100, the cationic surfactant cetyltrimethylammonium bromide (CTAB) and the anionic surfactant sodium N-lauroyl sarcosinate (SLS) on the decolorization of the reaction medium containing the monoazo dye Acid Orange 7 (AO7) by Alcaligenes faecalis and Rhodococcus erythropolis were studied. It was found that the surfactants influenced in different ways the rate of decolorization. At all concentrations tested the non-ionic surfactant Triton X-100 decreased the decolorization rate of R. erythropolis. At concentrations above the critical micelle concentration (CMC) Triton X-100 upset the usually observed exponential decay of the dye with A. faecalis due probably to the existence of an outer membrane in this organism. In concentrations above the CMC the anionic surfactant SLS inhibited the decolorization and, at prolonged incubation, caused partial release of the bound dye. The cationic surfactant CTAB in concentrations above and below the CMC accelerated drastically the binding of AO7 to the cells causing a rapid staining of the biomass and complete decolorization of the reaction medium. An attempt was made for explanation of the observed differences by the negative electrostatic charge of the living bacterial cell.

Acrylamide and Acrylic Acid Biodegradation by Alcaligenes faecalis 2 Planktonic Cells and Biofilms

2018 ◽  
Vol 54 (2) ◽  
pp. 173-178 ◽  
Author(s):  
Yu. G. Maksimova ◽  
D. M. Vasil’ev ◽  
A. S. Zorina ◽  
G. V. Ovechkina ◽  
A. Yu. Maksimov
Keyword(s):  
Acrylic Acid ◽  
Alcaligenes Faecalis ◽  
Planktonic Cells

Operational Stability of a Hydropower Plant with a Pipe-Shaped Air-Cushion Surge Chamber

2020 ◽  
Author(s):  
Tingyu Xu ◽  
Jian Zhang ◽  
Sheng Chen ◽  
Wei He ◽  
xiaodong Yu ◽  
...  
Keyword(s):  
Volume Ratio ◽  
Operational Stability ◽  
Hydropower Plant ◽  
Water Volume ◽  
Stable Operation ◽  
Initial Water ◽  
Hydropower Plants ◽  
Small Load ◽  
Air Cushion ◽  
Horizontal Area

Abstract This paper theoretically analyzed the design and operating parameters of a pipe-shaped air-cushion surge chamber (PS-ACSC). A mathematical model for a small load disturbance in a hydropower plant containing the PS-ACSC was established to analyze the effects of the sensitivity of its initial horizontal area and the air-water volume ratio on the operational stability of the plant. The results showed that the PS-ACSC should occupy a critical horizontal area, and its initial water level and the initial air-water volume ratio should be within a certain range to ensure its own stable operation as well as that of the turbine units. The results of a case study showed that a hydropower plant containing the PS-ACSC is most stable when the initial air-water volume ratio ranges from 2.90:1 to 6.68:1. In addition, a hydropower plant containing the PS-ACSC delivers better performance than a horseshoe-shaped air-cushion surge chamber under the same conditions. This study contributes to the design and operational control of hydropower plants containing the PS-ACSC.

Soft ionic liquid based resistive memory characteristics in a two terminal discrete polydimethylsiloxane cylindrical microchannel

2020 ◽  
Vol 8 (38) ◽  
pp. 13368-13374
Author(s):  
Muhammad Umair Khan ◽  
Gul Hassan ◽  
Jinho Bae
Keyword(s):  
Ionic Liquid ◽  
Acrylic Acid ◽  
Sodium Hydroxide ◽  
Sodium Salt ◽  
Resistive Memory ◽  
Cylindrical Microchannel ◽  
Memory Characteristics ◽  
Poly Acrylic Acid

This paper proposes a novel soft ionic liquid (IL) electrically functional device that displays resistive memory characteristics using poly(acrylic acid) partial sodium salt (PAA-Na+:H2O) solution gel and sodium hydroxide (NaOH) in a thin polydimethylsiloxane (PDMS) cylindrical microchannel.

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