scholarly journals CHARACTERISTICS OF BACTERIA ISOLATED FROM THE MINER OF THE VERKHNEKAMSKY SALT DEPOSIT (PERM KRAI)

2020 ◽  
pp. 312-320
Author(s):  
A. A. Pyankova ◽  
◽  
D. I. Usanina ◽  
V. S. Aleev ◽  
S. M. Blinov ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
New Taxon ◽  
Salt Deposit ◽  
Decomposition Products ◽  
Clay Deposits ◽  
Perm Krai ◽  
High Level ◽  
The 16S Rrna Gene

29 strains of halophilic/halotolerant bacteria were isolated from clay deposits of brine-diverting workings and brine pits of the mine of the Verkhnekamsky salt deposit (Perm krai). As a result of phylogenetic analysis based on a comparison of the 16S rRNA gene sequences, it was found that the isolated cultures are representatives of the classes Gammaproteobacteria (Halomonadaceae and Salinisphaeraceae fami-lies) and Bacilli (family Bacillaceae). Three halophilic strains SHV2, RV14, and SWV1 were similar to the closest type strain of the Salinisphaera hydrothermalis species at the level of 95.94-96.62% (16S rRNA gene), which indicates that these strains belong to a new taxon. Most of the isolated bacteria of the families Halomonadaceae and Bacillaceae are extremophiles: they grow at pH 9-10 and high sa-linity (up to 250-270 g/L NaCl). Two halophilic strains of the genus Halomonas have been found to de-grade salicylic and benzoic acids (decomposition products of polyaromatic compounds), which makes them promising for use in biotechnologies for the restoration of contaminated areas with a high level of mineralization.

scholarly journals GENETIC AND ECOLOGICAL-PHYSIOLOGICAL CHARACTERISTICS OF BACTERIA OF THE GENUS SALINISPHAERA (CLASS GAMMAPROTEOBACTERIA) ISOLATED FROM THE VERKHNEKAMSKY SALT MINER

2021 ◽  
Author(s):  
Vladislav S. Aleev ◽  
◽  
Elena G. Plotnikova ◽  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
High Salinity ◽  
Halophilic Bacteria ◽  
Rrna Gene ◽  
New Taxon ◽  
16S Rrna Gene Sequences ◽  
Clay Deposits ◽  
The 16S Rrna Gene

5 strains of halophilic bacteria were isolated from clay deposits of brine-diverting workings and brine tanks of the mine of the Verkhnekamskoye salt deposit (Solikamsk, Perm Territory). Because of phylogenetic analysis based on a comparison of the 16S rRNA gene sequences, it was found that the isolated cultures are members of the Salinisphaeraceae family. Three halophilic strains SHV2, RV14, and SWV1 had a similarity with the closest type strain of the Salinisphaera hydrothermalis species at the level of 95.94-96.62% (16S rRNA gene), which indicates that these strains belong to a new taxon. All isolated bacteria are halophiles: they grow at high salinity (up to 270-300 g / l NaCl).

Desmonostoc danxiaense sp. nov. (Nostocales, Cyanobacteria) from Danxia mountain in China based on polyphasic approach

Phytotaxa ◽  
2018 ◽  
Vol 367 (3) ◽  
pp. 233 ◽  
Author(s):  
FANGFANG CAI ◽  
YIMING YANG ◽  
QIANZHI WEN ◽  
RENHUI LI
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Southern China ◽  
Molecular Data ◽  
Rrna Gene ◽  
New Taxon ◽  
Cyanobacterial Species ◽  
Internally Transcribed Spacer ◽  
The 16S Rrna Gene

The recently established genus Desmonostoc was characterized by forming macroscopic colonies with diffluent mucilaginous envelopes embedding long vegetative filaments and possessing long chains of akinetes. The establishment of this new genus was further supported by the clustering of the 16S rRNA gene, which have a distinctive phylogenetic placement outside of Nostoc. In this study, a new cyanobacterial species was isolated from a wet rocky wall in Danxia mountain, Guangdong province, southern China, and the novel strains of this new species were evaluated by combining morphological characteristic and molecular data on the 16S rRNA gene and 16S-23S internally transcribed spacer (ITS). This new taxon was found to be closest to Desmonostoc species. The separation of the new species described here as Desmonostoc danxiaense, using morphological and molecular traits, was based on differences in phenotypic, 16S rRNA gene, ITS sequence and its secondary structure.

scholarly journals Molecular and biological characteristics of streptomyces diversity in the soils of the Saxaul forest in Mongolia

2021 ◽  
Vol 22 (1) ◽  
pp. 85-92
Author(s):  
J. Norovsuren ◽  
Liu Shao-Wei ◽  
Sun Cheng-Hang ◽  
B. Altansukh ◽  
Ch. Dorjsuren
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Isolation Methods ◽  
High Level ◽  
Type Strains ◽  
The 16S Rrna Gene ◽  
Ecological Range ◽  
Streptomyces Genus

Saxaul (Haloxylon ammodendron) is a plant with a wide ecological range that forms forests in Mongolia often used as pastures for camels. Actinomycetes were isolated from the soils of the saxaul forest using selective isolation methods. According to the results of phylogenetic analysis based on the sequences of the 16S rRNA gene fragment, it has been established that the obtained isolates belong to the Streptomyces genus. According to the taxonomic position, strains (M41; M42; M 43; C 4-46; C 4-47; C 4-50; M44; C 5-54; C5-60 and C 5-63) demonstrate a high level of similarity (99.20- 100 %) of 16S rRNA gene sequences with type strains of the following species: S. fradiae, S. huasconensis, S. coeruleoprunus, S. tendae, S. rubrogriseus, S. malachitofuscus, S. flavoviridis S. pilosus, S. caelestis, S. azureus, S. fulvissimus, S. microflavus, S. griseussubsp. griseus, S. anulatus, S. cyaneofuscatus, S. luridiscabiei, S. halstedii, S. fulvorobeus, S. pratensis, S. setonii, S. anulatus, S. pratensis, S. caelestis and S. azureus.

scholarly journals Association between 16S rRNA gene mutations and susceptibility to amikacin in Mycobacterium avium Complex and Mycobacterium abscessus clinical isolates

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Su-Young Kim ◽  
Dae Hun Kim ◽  
Seong Mi Moon ◽  
Ju Yeun Song ◽  
Hee Jae Huh ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Mycobacterium Avium ◽  
Inhibitory Concentration ◽  
Gene Mutations ◽  
Mycobacterium Abscessus ◽  
Clinical Isolates ◽  
Rrna Gene ◽  
High Level ◽  
Culture Conversion

AbstractWe evaluated the association between 16S rRNA gene (rrs) mutations and susceptibility in clinical isolates of amikacin-resistant nontuberculous mycobacteria (NTM) in NTM-pulmonary disease (PD) patients. Susceptibility was retested for 134 amikacin-resistant isolates (minimum inhibitory concentration [MIC] ≥ 64 µg/ml) from 86 patients. Amikacin resistance was reconfirmed in 102 NTM isolates from 62 patients with either Mycobacterium avium complex-PD (MAC-PD) (n = 54) or M. abscessus-PD (n = 8). MICs and rrs mutations were evaluated for 318 single colonies from these isolates. For the 54 MAC-PD patients, rrs mutations were present in 34 isolates (63%), comprising all 31 isolates with amikacin MICs ≥ 128 µg/ml, but only three of 23 isolates with an MIC = 64 µg/ml. For the eight M. abscessus-PD patients, all amikacin-resistant (MIC ≥ 64 µg/ml) isolates had rrs mutations. In amikacin-resistant isolates, the A1408G mutation (n = 29) was most common. Two novel mutations, C1496T and T1498A, were also identified. The culture conversion rate did not differ by amikacin MIC. Overall, all high-level and 13% (3/23) of low-level amikacin-resistant MAC isolates had rrs mutations whereas mutations were present in all amikacin-resistant M. abscessus isolates. These findings are valuable for managing MAC- and M. abscessus-PD and suggest the importance of phenotypic and genotypic susceptibility testing.

scholarly journals Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 781-786 ◽  
Author(s):  
Maximo Sánchez ◽  
Martha-Helena Ramírez-Bahena ◽  
Alvaro Peix ◽  
María J. Lorite ◽  
Juan Sanjuán ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Lotus Corniculatus ◽  
Culture Conditions ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The 16S Rrna Gene

Strain S658T was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).

scholarly journals Diagnosis of Fulminant Pneumonia Caused by Legionella pneumophila Serogroup 8 with the Sequence Analysis of the 16S rRNA Gene

2011 ◽  
Vol 225 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Toshinori Kawanami ◽  
Kazuhiro Yatera ◽  
Kazumasa Fukuda ◽  
Kei Yamasaki ◽  
Masamizu Kunimoto ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Legionella Pneumophila ◽  
Rrna Gene ◽  
The 16S Rrna Gene

scholarly journals The Western Progression of Lyme Disease: Infectious and NonclonalBorrelia burgdorferi Sensu LatoPopulations in Grand Forks County, North Dakota

2014 ◽  
Vol 81 (1) ◽  
pp. 48-58 ◽  
Author(s):  
Brandee L. Stone ◽  
Nathan M. Russart ◽  
Robert A. Gaultney ◽  
Angela M. Floden ◽  
Jefferson A. Vaughan ◽  
...  
Keyword(s):  
Lyme Disease ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
North Dakota ◽  
Intergenic Spacer ◽  
Rrna Gene ◽  
Content Type ◽  
Intergenic Spacer Region ◽  
Grand Forks ◽  
The 16S Rrna Gene

ABSTRACTScant attention has been paid to Lyme disease,Borrelia burgdorferi,Ixodes scapularis, or reservoirs in eastern North Dakota despite the fact that it borders high-risk counties in Minnesota. Recent reports ofB. burgdorferiandI. scapularisin North Dakota, however, prompted a more detailed examination. Spirochetes cultured from the hearts of five rodents trapped in Grand Forks County, ND, were identified asB. burgdorferi sensu latothrough sequence analyses of the 16S rRNA gene, the 16S rRNA gene-ileTintergenic spacer region,flaB,ospA,ospC, andp66. OspC typing revealed the presence of groups A, B, E, F, L, and I. Two rodents were concurrently carrying multiple OspC types. Multilocus sequence typing suggested the eastern North Dakota strains are most closely related to those found in neighboring regions of the upper Midwest and Canada. BALB/c mice were infected withB. burgdorferiisolate M3 (OspC group B) by needle inoculation or tick bite. Tibiotarsal joints and ear pinnae were culture positive, andB. burgdorferiM3 was detected by quantitative PCR (qPCR) in the tibiotarsal joints, hearts, and ear pinnae of infected mice. Uninfected larvalI. scapularisticks were able to acquireB. burgdorferiM3 from infected mice; M3 was maintained inI. scapularisduring the molt from larva to nymph; and further, M3 was transmitted from infectedI. scapularisnymphs to naive mice, as evidenced by cultures and qPCR analyses. These results demonstrate that isolate M3 is capable of disseminated infection by both artificial and natural routes of infection. This study confirms the presence of unique (nonclonal) and infectiousB. burgdorferipopulations in eastern North Dakota.

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