Molecularly imprinted sorbent for biological fluids sample preparation in forensic toxicology

2021 ◽  
Vol 64 (6) ◽  
pp. 39
Author(s):  
A.V. Voronin ◽  
T.L. Malkova ◽  
I.V. Synbulatov
Keyword(s):  
Sample Preparation ◽  
Biological Fluids ◽  
Forensic Toxicology ◽  
Molecularly Imprinted

Molecularly Imprinted Sensor for Ascorbic Acid Based on Gold Nanoparticles and Multiwalled Carbon Nanotubes

2020 ◽  
Vol 16 (7) ◽  
pp. 905-913
Author(s):  
Youyuan Peng ◽  
Qingshan Miao
Keyword(s):  
Carbon Nanotubes ◽  
Gold Nanoparticles ◽  
Ascorbic Acid ◽  
Multiwalled Carbon Nanotubes ◽  
Ph Value ◽  
Biological Fluids ◽  
Water Soluble ◽  
Multiwalled Carbon ◽  
Experimental Conditions ◽  
Molecularly Imprinted

Background: L-Ascorbic acid (AA) is a kind of water soluble vitamin, which is mainly present in fruits, vegetables and biological fluids. As a low cost antioxidant and effective scavenger of free radicals, AA may help to prevent diseases such as cancer and Parkinson’s disease. Owing to its role in the biological metabolism, AA has also been utilized for the therapy of mental illness, common cold and for improving the immunity. Therefore, it is very necessary and urgent to develop a simple, rapid and selective strategy for the detection of AA in various samples. Methods: The molecularly imprinted poly(o-phenylenediamine) (PoPD) film was prepared for the analysis of L-ascorbic acid (AA) on gold nanoparticles (AuNPs) - multiwalled carbon nanotubes (MWCNTs) modified glass carbon electrode (GCE) by electropolymerization of o-phenylenediamine (oPD) and AA. Experimental parameters including pH value of running buffer and scan rates were optimized. Scanning electron microscope (SEM), fourier-transform infrared (FTIR) spectra, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were utilized for the characterization of the imprinted polymer film. Results: Under the selected experimental conditions, the DPV peak currents of AA exhibit two distinct linear responses ranging from 0.01 to 2 μmol L-1 and 2 to 100 μmol L-1 towards the concentrations of AA, and the detection limit was 2 nmol L-1 (S/N=3). Conclusion: The proposed electrochemical sensor possesses excellent selectivity for AA, along with good reproducibility and stability. The results obtained from the analysis of AA in real samples demonstrated the applicability of the proposed sensor to practical analysis.

Synthesis and characterization of a new magnetic restricted access molecularly imprinted polymer for biological sample preparation

2020 ◽  
Vol 24 ◽  
pp. 101002 ◽  
Author(s):  
Tássia Venga Mendes ◽  
Lidiane Silva Franqui ◽  
Mariane Gonçalves Santos ◽  
Célio Wisniewski ◽  
Eduardo Costa Figueiredo
Keyword(s):  
Sample Preparation ◽  
Molecularly Imprinted Polymer ◽  
Biological Sample ◽  
Synthesis And Characterization ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Restricted Access

scholarly journals The Use of Infrared Spectrophotometry for Measuring Body Water Spaces

1999 ◽  
Vol 45 (7) ◽  
pp. 1077-1081 ◽  
Author(s):  
Graham Jennings ◽  
Leslie Bluck ◽  
Antony Wright ◽  
Marinos Elia
Keyword(s):  
Sample Preparation ◽  
Total Body Water ◽  
Biological Fluids ◽  
Deuterium Oxide ◽  
Body Water ◽  
The Body ◽  
Infrared Spectrophotometry ◽  
Significant Difference ◽  
Total Body ◽  
Novel Algorithm

Abstract Background: The conventional method of measuring total body water by the deuterium isotope dilution method uses gas isotope ratio mass spectrometry (IRMS), which is both expensive and time-consuming. We investigated an alternative method, using Fourier transform infrared spectrophotometry (FTIR), which uses less expensive instrumentation and requires little sample preparation. Method: Total body water measurements in human subjects were made by obtaining plasma, saliva, and urine samples before and after oral dosing with 1.5 mol of deuterium oxide. The enrichments of the body fluids were determined from the FTIR spectra in the range 1800–2800 cm−1, using a novel algorithm for estimation of instrumental response, and by IRMS for comparison. Results: The CV (n = 5) for repeat determinations of deuterium oxide in biological fluids and calibrator solutions (400–1000 μmol/mol) was found to be in the range 0.1–0.9%. The use of the novel algorithm instead of the integration routines supplied with the instrument gave at least a threefold increase in precision, and there was no significant difference between the results obtained with FTIR and those obtained with IRMS. Conclusion: This improved infrared method for measuring deuterium enrichment in plasma and saliva requires no sample preparation, is rapid, and has potential value to the clinician.

scholarly journals Chemical and Toxicological Analysis of Antiretroviral Drugs

2019 ◽  
Vol 8 (4) ◽  
pp. 53-60
Author(s):  
T. N. Komarov ◽  
M. V. Belova ◽  
D. D. Stolyarova ◽  
I. E. Shohin ◽  
D. S. Bogdanova ◽  
...  
Keyword(s):  
Side Effects ◽  
Sample Preparation ◽  
Highly Active Antiretroviral Therapy ◽  
High Performance ◽  
Biological Fluids ◽  
Screening Method ◽  
Acute Poisoning ◽  
Antiretroviral Drugs ◽  
Precipitation Method ◽  
Relevant Today

Introduction. Human Immunodeficiency Virus (HIV) is one of the main socially significant infection all over the world. HIV-positive patients take medical care, including antiretroviral drugs (ARVs) pharmacotherapy. Like all drugs, ARVs have lots of side effects that should be taken when prescribing drugs as part of highly active antiretroviral therapy. There are many cases when side effects of ARVs caused patients to enter the toxicology department. Therefore, the development of new methods for the analysis of ARV in biological fluids for the timely diagnosis of treatment of poisoning of this group of drugs is relevant today.Aim. The aim of this study is development of screening analysis of atazanavir, abacavir, nevirapine, ritonavir, lopinavir, zidovudine, darunavir and efavirenz in the urine to identify these drugs as possible toxicants for poisoning by high-performance liquid chromatography with tandem massselective detection (HPLC-MS/MS).Materials and methods. Identification of ARV was performed by HPLC-MS/MS. Methanol precipitation method was used as a sample preparation.Results and discussion. The optimal conditions for sample preparation, chromatographic separation, and mass-spectrometric detection were selected to determine the studied ARVs. This method was tested on urine samples from patients in the Department of Acute Poisoning and Somatopsychiatric Disorders (OOSPD) with acute ARV poisoning.Conclusion. This screening method for analyse atazanavir, abacavir, nevirapine, ritonavir, lopinavir, zidovudine, darunavir and efavirenz in human urine has been developed by HPLC-MS/MS. The developed method can be used to identify these drugs as possible toxicants in case of poisoning. The prospect for the development of the topic is the inclusion of new molecules in the method and quantitative determination of the studied ARVs. 

Computational design, synthesis and utilization of a magnetic molecularly imprinted polymer on graphene oxide nanosheets for highly selective extraction and determination of buprenorphine in biological fluids and tablets

2018 ◽  
Vol 10 (43) ◽  
pp. 5214-5226 ◽  
Author(s):  
Farideh Ganjavi ◽  
Mehdi Ansari ◽  
Maryam Kazemipour ◽  
Leila Zeidabadinejad
Keyword(s):  
Biological Fluids ◽  
Computational Design ◽  
Selective Extraction ◽  
Graphene Oxide Nanosheets ◽  
Imprinted Polymer ◽  
Design Synthesis ◽  
Molecularly Imprinted ◽  
Plasma And Urine Samples ◽  
Computational Design Synthesis

A magnetic MIP for the selective extraction of buprenorphine (BUP) from real plasma and urine samples and tablets based on computational design as a novel procedure has been developed.

Sign in / Sign up

Export Citation Format

Share Document