scholarly journals Anatomía de la semilla de Chenopodium berlandieri ssp. nuttalliae (Chenopodiaceae) "huauzontle"

2017 ◽  
pp. 17
Author(s):  
Aida Carrillo-Ocampo ◽  
E. Mark Engleman
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Seed Coat ◽  
Outer Integument ◽  
Three Dimensional ◽  
Mature Fruit ◽  
Dimensional Network ◽  
Chenopodium Berlandieri ◽  
Scanning Electron

The seed of huauzontle (Chenopodium berlandieri ssp. nuttalliae) was studied by light microscopy and scanning electron microscopy. When the outer integument arises around the young ovule, instead of covering the inner integument and the nucellus, it grows backwards and partially surrounds the funiculus . When the pericarp is removed from the mature fruit, the seed is straw colored, because only the tegmen covers the seed. The chalaza of this seed has the form of a truncate cone, with the elliptical base towards the nucellus. In this zone of contact between the chalaza and the nucellus. a cuticle is deposited that surrounds some cells and makes a three dimensional network. This chalazal network is in contact with a smooth nucellar cuticle that fom1s part of the seed coat. The inversion of the inner integument could represent a selected mutation during the process of domestication.

Taxonomic application of macro and micro morphological characters of seeds in Astragalus L. (Galegeae, Fabaceae) in India

Phytotaxa ◽  
2021 ◽  
Vol 502 (2) ◽  
pp. 191-207
Author(s):  
SHIVANI KASHYAP ◽  
CHANDAN KUMAR SAHU ◽  
ROHIT KUMAR VERMA ◽  
LAL BABU CHAUDHARY
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Seed Coat ◽  
Morphological Plasticity ◽  
Morphological Characters ◽  
Individual Species ◽  
Large Size ◽  
The Individual ◽  
Scanning Electron

Due to large size and enormous morphological plasticity, the taxonomy of the genus Astragalus is very complex and challenging. The identification and grouping of species chiefly based on macromorphological characters become sometimes difficult in the genus. In the present study, the micromorphology of the seeds of 30 species belonging to 14 sections of Astragalus from India has been examined applying scanning electron microscopy (SEM) along with light microscopy (LM) to evaluate their role in identification and classification. Attention was paid to colour, shape, size and surface of seeds. The overall size of the seeds ranges from 1.5–3.2 × 0.8–2.2 mm. The shape of the seeds is cordiform, deltoid, mitiform, orbicular, ovoid and reniform. The colour of seeds varies from brown to blackish-brown to black. Papillose, reticulate, ribbed, rugulate and stellate patterns were observed on the seed coat surface (spermoderm) among different species. The study reveals that the seed coat ornamentations have evolved differently among species and do not support the subgeneric and sectional divisions of the genus. However, they add an additional feature to the individual species, which may help in identification in combination with other macro-morphological features.

scholarly journals Structure and microstructure of coronary dentin in non-erupted human deciduous incisor teeth

2002 ◽  
Vol 13 (3) ◽  
pp. 170-174 ◽  
Author(s):  
Luciane R.R S. Costa ◽  
Ii-Sei Watanabe ◽  
Márcia C. Kronka ◽  
Marcelo C.P. Silva
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nitric Acid ◽  
Light Microscopy ◽  
Three Dimensional ◽  
Dentinal Tubules ◽  
Sem Study ◽  
Structure And Microstructure ◽  
Hematoxylin Eosin ◽  
Scanning Electron

The dentin structure of non-erupted human deciduous mandibular and maxillary central and lateral incisor teeth was studied employing light and scanning electron microscopy. For light microscopy, nitric-acid-demineralized and ground sections were used. The sections were stained by hematoxylin-eosin, picrosirius and azo-carmim methods, and ground specimens were prepared using a carborundum disk mounted in a handpiece. For SEM study, teeth were frozen in liquid nitrogen and fractured at longitudinal and transversal directions. Structurally, demineralization and ground methods revealed tubules with primary and secondary curvatures, canaliculi, giant tubules, interglobular dentin, predentin, and intertubular dentin. Scanning electron microscopy showed three-dimensional aspects of dentinal tubules, canaliculi, peritubular dentin, intertubular dentin, and predentin. This study contributes to knowledge about dentin morphology showing characteristics of teeth not yet submitted to mastication stress.

Caracterización microestructural del queso de Burgos mediante diferentes técnicas microscópicas / Microstructural characterization of Burgos cheese using different microscopy techniques

2000 ◽  
Vol 6 (2) ◽  
pp. 151-157 ◽  
Author(s):  
I. Hernando ◽  
I. Pérez-Munuera ◽  
M.A. Lluch
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Three Dimensional ◽  
Microstructural Characterization ◽  
Transmission Electron ◽  
Dimensional Network ◽  
Lining Structure ◽  
Cheese Microstructure ◽  
Microscopy Techniques ◽  
Scanning Electron

Electron microscopy has made a significant contribution to our knowledge of the structure of foods and the interaction among their components. In this paper, several electron microscopy techniques are applied to study the Burgos cheese microstructure. Burgos cheese samples fixed in glutaraldehyde and observed by scanning electron microscopy showed a continuous three-dimensional network of protein, with roundish empty spaces, which probably contained fat, whey or air in the original sample. Fixation in osmium tetroxide showed the distribution of fat, which is organized in globules (1-3 ltm in diameter). Water closely and uniformly interacting with proteins and the protein shells deposited around the fat globule membranes (0.2 pm thick) can be observed by cryo-scanning electron microscopy. Samples observed by transmission electron microscopy showed loosely or ' strongly aggregated proteins forming the continuous network. Furthermore, a core and lining structure were distinguished; this structure could be related to the presence of B-lactoglobulin. Finally, this technique allows observations of individual casein grains and the interstitial spaces among them.

Application of Scanning Electron Microscopy to paraffin-embedded plant tissues to study invasive processes of plant-pathogenic fungi

1986 ◽  
Vol 44 ◽  
pp. 282-283
Author(s):  
E. G. Kokko ◽  
D. A. Gaudet
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Pathogenic Fungus ◽  
Three Dimensional ◽  
Pathogenic Fungi ◽  
Great Depth ◽  
Development Processes ◽  
Wheat Leaves ◽  
Scanning Electron

Scanning electron microscopy (SEM) applied to paraffin-embedded tissue section is compared with the traditional techniques of light microscopy (LM) and surface SEM for the study of invasion by a plant-pathogenic fungus. SEM of paraffin-embedded sections of wheat leaves infected by Coprinus psychromorbidus consistently yielded high-quality micrographs showing three-dimensional views of both internal and external disease development processes. When the orientation of the specimen in the SEM is manipulated, the specimen can be viewed from different perspectives. The technique is simple and inexpensive and combines the advantages of great depth of focus and high resolution of the SEM with the simple preparatory techniques employed for light microscopy.

scholarly journals Microscopic study of edema in hydatidiform mole

2014 ◽  
Vol 14 (3) ◽  
pp. 261-268
Author(s):  
Olivar C. Castejón ◽  
Aury Caraballo ◽  
Oliver Castejón ◽  
Elizabeth Cedeño
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basement Membrane ◽  
Light Microscopy ◽  
Hydatidiform Mole ◽  
Three Dimensional ◽  
Wide Range ◽  
Observation Protocol ◽  
Microscopy Techniques ◽  
Scanning Electron

Objectives: the purpose of this study is to use light microscopy and scanning electron microscopy to determine the effect of edema on the structure of the molar vesicle. Methods: samples were taken from the complete hydatidiform mole and processed using conventional light and scanning electron microscopy techniques and an observation protocol that identified four variables: factors underlying the development of edema; the condition of the trophoblast basement membrane, development of the villi, accumulation and degeneration of sulphated mucosubstances at stromal level. Results: light microscopy showed a permeable trophoblastic basement membrane, a swollen syncytium, edematous regions disorganizating the stromal region and causing ischemic necrosis of cells. Using scanning electron microscopy, the basement membrane was found to be distended and thickened, with large irregular holes for the entry and movement of liquid, leaving a wide range of fluids during the influx process and depriving stromal cells of nutrition. Conclusions: a new three-dimensional view of the changes brought about by the entry of fluids into the stroma of molar hydropic vesicles was provided by scanning electron microscopy and confirmed by light microscopy, thereby explaining the changes occurring at the level of the stroma as an effect of the edema.

Three-Dimensional Morphology of Microdamage in Peri-Screw Bone: A Scanning Electron Microscopy of Methylmethacrylate Cast Replica

2012 ◽  
Vol 18 (5) ◽  
pp. 1106-1111 ◽  
Author(s):  
Lei Wang ◽  
Jin Shao ◽  
Tingjun Ye ◽  
Lianfu Deng ◽  
Shijing Qiu
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fluorescence Microscopy ◽  
Light Microscopy ◽  
Three Dimensional ◽  
Basic Fuchsin ◽  
Bright Field ◽  
Different Types ◽  
Surrounding Bone ◽  
Scanning Electron

AbstractScrew implantation inevitably causes microdamage in surrounding bone. However, little is known about the detailed characteristics of microdamage in peri-screw bone. In this study, we developed a method to construct microdamage cast with methylmethacrylate (MMA) and observed the cast using scanning electron microscopy (SEM). In basic fuchsin stained bone sections observed by bright-field and fluorescence microscopy, diffuse damage, cross-hatched damage, and linear cracks were all presented in peri-screw bone. Using MMA casting/SEM method, we found numerous densely packed microcracks in the areas with diffuse damage. The osteocyte canaliculi and the microcracks consisting of diffuse damage had a similar diameter (or width), usually <0.5 μm, but their morphology was largely different. In the area with cross-hatched damage, the orientation of microcracks was similar to that in diffuse damage, but the number was significantly decreased. Many microcracks were thicker than 1 μm and associated with a rough surface. Large linear cracks (∼10 μm in diameter) occurred in different areas. Plenty of microcracks were present on the surface of some linear cracks. In conclusion, the MMA casting/SEM method can demonstrate the three-dimensional morphology of different types of microdamage, particularly the microcracks in diffuse damage, which are unable to be shown by light microscopy.

scholarly journals Description of some characteristics of flowers and seeds of Arabidopsis thaliana - ecotype landsberg erecta and mutant NW4

2014 ◽  
Vol 65 (1-2) ◽  
pp. 171-174
Author(s):  
Leszek Trząski ◽  
Jagna Karcz ◽  
Alicja Miszta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Arabidopsis Thaliana ◽  
Light Microscopy ◽  
Seed Coat ◽  
Variable Number ◽  
Characteristic Features ◽  
Seed Coat Morphology ◽  
Scanning Electron

Flowers and seeds of Landsberg <em>erecta</em> (Ler) ecotype and NW4 mutant were studied by light microscopy and scanning electron microscopy to reveal characteristic features of their structure. The NW4 mutant flowers differ from Ler mainly in presence of two bract-like sepals with complicated vasculature and a variable number of secondary flowers. In the two outer whorls of NW4 flower, variable number of transformed stamen-, petal-, sepal- and style-like elements also occur. The NW4 mutant seeds are characterized by the absence of mucilage around the surface and a deviating seed coat morphology.

scholarly journals Synthesis of Antibacterial Gelatin/Sodium Alginate Sponges and Their Antibacterial Activity

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1926
Author(s):  
Yanyi Wen ◽  
Bing Yu ◽  
Zhongjie Zhu ◽  
Zhuoran Yang ◽  
Wei Shao
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Antibacterial Activity ◽  
Three Dimensional ◽  
Gram Negative Bacteria ◽  
High Porosity ◽  
Gram Negative ◽  
Good Antibacterial Activity ◽  
Dimensional Network ◽  
Scanning Electron

In the present study, sponges with the antibiotic tetracycline hydrochloride (TCH) loaded into alginate incorporated with gelatin (G/SA) were fabricated. The G/SA sponges were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric (TG) analysis. G/SA sponges show a three-dimensional network structure with high porosity. An excellent swelling behavior and a controlled TCH release performance are observed from G/SA sponges. Moreover, they exhibit good antibacterial activity against both Gram-positive and Gram-negative bacteria.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

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