scholarly journals Electronmicroscopic study of Beet soilborne pomovirus

2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 255-257
Author(s):  
P. Kudláčková ◽  
M. Zouhar ◽  
P. Ryšánek
Keyword(s):  
Colloidal Gold ◽  
Polyclonal Antibodies ◽  
Chenopodium Quinoa ◽  
Acrylic Resin ◽  
Virus Particles ◽  
Electronmicroscopic Study ◽  
Lr White ◽  
Local Lesions ◽  
Number Of Particles

Beet soilborne pomovirus (BSBV) was observed both in the sap and in tissues from local lesions on Chenopodium quinoa leaves after their embedding into acrylic resin LR White. Immunocapturing with polyclonal antibodies was used to enhance number of particles on grids and immunolabelling by colloidal gold was used for better visibility of virus particles in tissues. BSBV has rod-like particles of various length and it forms inclusions of several particles adhering side to side each to another.

scholarly journals AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF A MOUSE HEPATITIS VIRUS IN TISSUE CULTURE CELLS

1965 ◽  
Vol 24 (1) ◽  
pp. 57-78 ◽  
Author(s):  
J. F. David-Ferreira ◽  
R. A. Manaker
Keyword(s):  
Electron Microscope ◽  
Inclusion Bodies ◽  
Hepatitis Virus ◽  
Mouse Hepatitis Virus ◽  
Dense Material ◽  
Virus Particles ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Tubular Body ◽  
Number Of Particles

Samples taken at different intervals of time from suspension cultures of the NCTC 1469 line of mouse liver—derived (ML) cells infected with a mouse hepatitis virus have been studied with the electron microscope. The experiments revealed that the viruses are incorporated into the cells by viropexis within 1 hour after being added to the culture. An increasing number of particles are found later inside dense cytoplasmic corpuscles similar to lysosomes. In the cytoplasm of the cells from the samples taken 7 hours after inoculation, two organized structures generally associated and never seen in the controls are observed: one consists of dense material arranged in a reticular disposition (reticular inclusion); the other is formed by small tubules organized in a complex pattern (tubular body). No evidence has been found concerning their origin. Their significance is discussed. With the progression of the infection a system of membrane-bounded tubules and cisternae is differentiated in the cytoplasm of the ML cells. In the lumen of these tubules or cisternae, which are occupied by a dense material, numerous virus particles are observed. The virus particles which originate in association with the limiting membranes of tubules and cisternae are released into their lumen by a "budding" process. The virus particles are 75 mµ in diameter and possess a nucleoid constituted of dense particles or rods limiting an electron transparent core. The virus limiting membrane is sometimes covered by an outer layer of a dense material. In the cells from the samples taken 14 to 20 hours after inoculation, larger zones of the cell cytoplasm are occupied by inclusion bodies formed by channels or cisternae with their lumens containing numerous virus particles. In the samples taken 20 hours or more after the inoculation numerous cells show evident signs of degeneration.

scholarly journals Coat protein and RNAs of Cole latent virus are not present within chloroplasts of Chenopodium quinoa-infected cells

2003 ◽  
Vol 28 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Priscila Belintani ◽  
José O. Gaspar
Keyword(s):  
Electron Microscopy ◽  
Coat Protein ◽  
Chenopodium Quinoa ◽  
Latent Virus ◽  
Percoll Gradient ◽  
Northern Blots ◽  
Virus Particles ◽  
Ultrastructural Studies ◽  
Particle Aggregates ◽  
Infected Cells

Cole latent virus (CoLV), genus Carlavirus, was studied by electron microscopy and biochemical approaches with respect both to the ultrastructure of the Chenopodium quinoa infected cells and to its association with chloroplasts. The CoLV was observed to be present as scattered particles interspersed with membranous vesicles and ribosomes or as dense masses of virus particles. These virus particles reacted by immunolabelling with a polyclonal antibody to CoLV. Morphologically, chloroplasts, mitochondria and nuclei appeared to be unaltered by virus infection and virus particles were not detected in these organelles. However, virus particle aggregates were frequently associated with the outer membrane of chloroplasts and occasionally with peroxisomes. Chloroplasts were purified by Percoll gradient, and the coat protein and virus-associated RNAs were extracted and analyzed by Western and Northern blots respectively. Coat protein and CoLV-associated RNAs were not detected within this organelle. The results presented in this work indicate that the association CoLV/chloroplasts, observed in the ultrastructural studies, might be a casual event in the host cell, and that the virus does not replicate inside the organelle.

scholarly journals Addition of phosphotungstic acid to ethanol for dehydration improves both the ultrastructure and antigenicity of pituitary tissue embedded in LR White acrylic resin

2005 ◽  
Vol 68 (5) ◽  
pp. 337-347 ◽  
Author(s):  
Yuko Sakai ◽  
Masahiro Hosaka ◽  
Yoshiki Hira ◽  
Tsuyoshi Watanabe
Keyword(s):  
Phosphotungstic Acid ◽  
Acrylic Resin ◽  
Pituitary Tissue ◽  
Lr White

Obtainment of Polyclonal Antibodies to Clenbuterol with the Use of Colloidal Gold

2007 ◽  
Vol 29 (3-4) ◽  
pp. 563-568 ◽  
Author(s):  
O.A. Vasilenko ◽  
S.A. Staroverov ◽  
D.N. Yermilov ◽  
D.V. Pristensky ◽  
S. Yu. Shchyogolev ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Polyclonal Antibodies

TWO VIRUS DISEASES OF RHUBARB IN EASTERN ONTARIO

1960 ◽  
Vol 40 (1) ◽  
pp. 104-109 ◽  
Author(s):  
D. S. MacLachlan
Keyword(s):  
Nicotiana Tabacum ◽  
Phaseolus Vulgaris ◽  
Phosphate Buffer ◽  
Datura Stramonium ◽  
Vigna Sinensis ◽  
Virus Diseases ◽  
Virus Particles ◽  
Local Lesions ◽  
Necrotic Spots ◽  
Virus Strains

Two viruses were isolated from Ruby Red rhubarb growing in the Ottawa district. Isolate I produced a severe rugosity and mottle on rhubarb, a systemic mottle in Nicotiana tabacum, N. glutinosa and Datura stramonium, and local lesions on Phaseolus vulgaris, Vigna sinensis and Beta vulgaris. The virus was inactivated after being heated for 10 minutes at 65 °C., and became non-infective after the sap was diluted to 1:2000. Isolate II incited the formation of chlorotic and necrotic spots on rhubarb, and produced a systemic mottle in N. tabacum, N. glutinosa, and D. stramonium. The virus was inactivated by being heated at 75 °C. for ten minutes, and became non-infective after the sap was diluted 1:8000. Both isolates were mechanically transmitted by diluting crude sap 1;100 in 0.1 M. phosphate buffer (pH 8–9) and rubbing on leaves dusted with carborundum. The success in isolating these viruses or virus strains was dependent on the season in which isolations were attempted. The size of the virus particles of both isolates was 478 × 15μm.

Electron Microscopic Study of the Graffi Virus in Bone Marrow of Leukemic Mice.

1966 ◽  
Vol 52 (1) ◽  
pp. 35-59 ◽  
Author(s):  
Natale Pennelli ◽  
Luciano Fiore-Donati ◽  
Luigi Chieco-Bianchi ◽  
Giuseppe Tridente
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Cells ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Negative Stain ◽  
Cytoplasmic Vesicles ◽  
C57bl Mice ◽  
Ultrathin Sections ◽  
Number Of Particles ◽  
Marrow Cells

Bone marrow from C57BL mice with myeloid leukemia induced by Graffi virus has been studied with the electron microscope by ultrathin section and negative stain techniques. Virus particles were usually found in different types of bone marrow cells as well as in extracellular spaces. However, the highest number of particles in various stages of maturation was observed in the cytoplasm of megakaryocytes. Two main types of virus particle were found: the immature Al particle and the mature C particle. They were morphologically indistinguishable from other murine leukemogenic viruses. In partially purified preparations studied by negative staining, some of the particles which were not penetrated by PTA, frequently showed a tail-like structure of variable length. In ultrathin sections, particles were found to originate by budding from the cell membranes. Budding of particles was particularly evident in megakaryocytes and especially within the granules and cytoplasmic vesicles or in connection with the platelet demarcating membranes. The findings of a high number of virus particles in all stages of maturation in megakaryocytes together with a certain degree of megakaryocytosis observed in the bone marrow suggest that this type of cell is possibly one of the main source of production of the virus. A few particles resembling morphologically mycoplasma were detected within the cytoplasm of some immature bone marrow cells.

scholarly journals Purification and Properties of a New Virus from Black Currant, Its Affinities with Nepoviruses, and Its Close Association with Black Currant Reversion Disease

1997 ◽  
Vol 87 (4) ◽  
pp. 404-413 ◽  
Author(s):  
A. Lemmetty ◽  
S. Latvala ◽  
A. T. Jones ◽  
P. Susi ◽  
W. J. McGavin ◽  
...  
Keyword(s):  
Close Association ◽  
Chenopodium Quinoa ◽  
Severe Form ◽  
Causal Agent ◽  
Rt Pcr ◽  
Black Currant ◽  
Virus Particles ◽  
Virus Rna ◽  
Purification And Properties ◽  
Line Patterns

Black currant reversion is a virus-like disease whose causal agent has not been identified. In rooted cuttings of a black currant plant affected with the severe form of the disease, pronounced chlorotic line patterns and ringspots developed in newly emerging leaves. From such symptom-bearing leaves, a virus was mechanically transmitted with difficulty to Chenopodium quinoa and, from this host, to other herbaceous test plants. The virus was purified and partially characterized, and the purified viri-ons were used for antiserum production. Virus particles were isometric, approximately 27 nm in diameter, and sedimented as two nucleoprotein components. They contained a protein species with a molecular mass of 55 kDa, which was readily degraded into a 54-kDa protein and two major RNA components of about 6,700 and 7,700 nucleotides (nt), each with a poly(A) tail. Most of these properties are shared by nepoviruses, but the virus was serologically unrelated to 14 nepoviruses or putative nepovi-ruses tested. However, the deduced sequence of 1,260 nt at the 3′ end of one of the viral RNA species was distinct from any known viral sequence, except that it contained short regions of homology to the 3′ terminal sequences of RNAs of seven other nepoviruses and two comovi-ruses. To detect this virus in Ribes plants, primers were designed from the known sequence to amplify a 210-nt region of the cDNA of the virus RNA using an immunocapture reverse transcriptase polymerase chain reaction (IC-RT-PCR) protocol. Using this assay for the virus, we associated its presence with two recognized forms of black currant reversion disease occurring in Finland, Scotland, or New Zealand. We also detected the virus in vector gall mites from reverted plants and in black currant plants on which such vector mites had fed. However, the virus was not detected by IC-RT-PCR in known healthy Ribes plants; in Ribes plants free from reversion, but affected by three other distinct virus-like diseases of Ribes; or in plants infected with arabis mosaic, strawberry latent ringspot, or raspberry ringspot nepoviruses. These data suggest that this virus may be the causal agent of reversion disease, and it is tentatively called black currant reversion associated virus.

scholarly journals Natural Infection of Alstroemeria brasiliensis with Lily Mottle Virus

Plant Disease ◽  
2000 ◽  
Vol 84 (1) ◽  
pp. 103-103 ◽  
Author(s):  
I. Bouwen ◽  
R. A. A. van der Vlugt
Keyword(s):  
Polymerase Chain Reaction Amplification ◽  
Natural Infection ◽  
Tobacco Rattle Virus ◽  
Chenopodium Quinoa ◽  
Flower Color ◽  
Systemic Vein ◽  
Leaf Chlorosis ◽  
Local Lesions ◽  
Funded Project ◽  
Reaction Amplification

During a survey for a European Union-funded project on viruses of Alstroemeria, two A. brasiliensis plants were found expressing virus-like symptoms, including leaf chlorosis with deep-green oval spots and flower color breaking. In enzyme-linked immunosorbent assays (ELISA), no positive reaction was obtained with antisera to Alstroemeria mosaic, Alstroemeria carla, Cucumber mosaic, Freesia mosaic, or Tobacco rattle virus or potyvirus-specific monoclonal antibodies (Agdia, Elkhart, IN). ELISA reactions were positive with antisera to Lily mottle (LMoV) and Rembrandt tulip breaking viruses (1). In electron microscopy preparations of A. brasiliensis, potyvirus-like particles were observed. Using sap-inoculation, the virus was transferred to a range of host species. Chenopodium quinoa, Nicotiana occidentalis accession 37B, and N. occidentalis subsp. obliqua (P1) expressed local lesions; N. clevelandii expressed local and systemic mottle; and N. benthamiana expressed local lesions, systemic vein yellowing, and leaf crinkling. Isolated total RNA from infected N. benthamiana was used for initial cDNA synthesis and polymerase chain reaction amplification with a potyvirus-specific primer set (2). The amplicon (≈670 bp) was cloned and sequenced. The sequence showed 92% homology with the corresponding region of LMoV RNA (GenBank accession no. S44147). The results confirm the infection of A. brasiliensis with LMoV. This is the first report of natural infection of Alstroemeria by LMoV. References: (1) E. L. Dekker et al. J. Gen. Virol. 74:881, 1993. (2) R. A. A. van der Vlugt et al. Phytopathology 89:148, 1999.

Aggregation Studies: MR and MA With Poxvirus

1967 ◽  
Vol 25 ◽  
pp. 90-91
Author(s):  
D. G. Sharp ◽  
Kwang Soo Kim
Keyword(s):  
Cell Interaction ◽  
Actual State ◽  
New Host ◽  
Virus Particles ◽  
Particle Level ◽  
Dilute Suspension ◽  
The Individual ◽  
Quality Factor Q ◽  
Made In ◽  
Number Of Particles

The only instrument that gives direct information about virus-cell interaction at the individual particle level is the electron microscope. The counting of virus particles sedimented from dilute suspension for electron microscopy showed first that rather few of them produce plaques. Soon it was shown that the quality factor (Q=PFU/particles) was not a constant. It changed during the repeated passages required for adaptation of a virus to growth in a new host cell. More recently changes in Q have been observed when changes are made in the state of aggregation of the virus particles. These changes, their measurement and their meaning are the substance of this paper.Virus particles sedimented from dilute suspension fall upon a flat surface producing a pattern that shows coincidence pairs, triplets, etc., produced by chance falling together of separate particles. The number of these is small and it can be calculated. Aggregation in excess of this amount is true aggregation. It is the actual state of the virus particle population that encounters the cells of the monolayer on which plaques appear. From electron micrographs the frequency Ni of groups containing i particles is determined together with the total number of particles.

The Occurrence of “A,” “C” and “Spoked” Virus Particles in Hamster Tumors

1971 ◽  
Vol 29 ◽  
pp. 370-371
Author(s):  
H. Saito ◽  
B. G. Uzman
Keyword(s):  
Tumor Cells ◽  
Human Tumor ◽  
Outer Diameter ◽  
Kidney Cells ◽  
Human Tumor Cells ◽  
Virus Particles ◽  
Type C ◽  
Murine Leukemia ◽  
Baby Hamster Kidney Cells ◽  
Number Of Particles

Several passages of five different hamster tumor lines were surveyed by electron microscopy for the presence of virus particles. Four of these tumors originated and were serially transplanted in adult Syrian hamsters. One line was derived by implantation of cultured baby hamster kidney cells. In all 5 tumor lines examined, “spoked virus” particles, first described by Bernhard and Tournier, were observed. These particles (Fig. 1) have an outer diameter of 90-110 mµ with a central nucleoid of 40-50 mµ and are always found in cisternae of rough endoplasmic reticulum or within the outer nuclear envelope. They are easily distinguishable from the murine leukemia type C particles by the presence of characteristic electron-dense radial structures which emanate from the nucleoid. In the cell line which has been most extensively studied (H-Sul-1-SC), the number of particles per cell appeared to increase in later passages of the tumor. Tumor cells from this line were grown in culture and were found to carry the same particles. While the “spoked virus” has been found by many investigators in a variety of neoplastic hamster cells, it has not been observed in this laboratory in an extensive survey of human tumor cells serially transplanted in newborn hamsters.

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