scholarly journals Optimisation of ultrasonic-assisted protein extraction from brewer’s spent grain

2010 ◽  
Vol 28 (No. 1) ◽  
pp. 9-17 ◽  
Author(s):  
D.-S. Tang ◽  
Y.-J. Tian ◽  
Y.-Z. He ◽  
L. Li ◽  
S.-Q. Hu ◽  
...  
Keyword(s):  
Protein Extraction ◽  
Response Surfaces ◽  
Protein Yield ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Liquid Ratio ◽  
Brewer’S Spent Grain ◽  
Ultrasonic Assisted ◽  
Spent Grain ◽  
Solid Liquid

Response surface methodology was employed to optimise the ultrasonic-assisted extraction of protein from brewer’s spent grain. Three variables, namely the extraction time (min), ultrasonic power (W/100 ml of extractant), and solid-liquid ratio (g/100 ml) were investigated. Optimal conditions were determined and tri-dimensional response surfaces were plotted using mathematical models. The ANOVA analysis indicated that all the quantities determined, i.e. the extraction time, ultrasonic power, and solid-liquid ratio, had significant positive linear and negative quadratic effects on the protein yield. Optimum conditions for the extraction of protein were found to be: the extraction time of 81.4 min, ultrasonic power of 88.2 W/100 ml of extractant, and solid-liquid ratio of 2.0 g/100 ml. The optimal predicted protein yield obtained was 104.2 mg/g BSG while the experimental yield of protein was in agreement with the predicted value.

Study on Ultrasonic Assisted - Precipitation Method Combined Purification of Tea Saponin

2013 ◽  
Vol 634-638 ◽  
pp. 1552-1556 ◽  
Author(s):  
Yu Qiong Wu ◽  
Wei Dong Man
Keyword(s):  
Ethanol Concentration ◽  
Extraction Time ◽  
Precipitation Method ◽  
Optimum Operating ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Tea Saponin ◽  
Ultrasonic Assisted ◽  
Solid Liquid

Commercial crude tea saponin as raw material, ultrasonic assisted extraction technology of tea saponin was investigated. Five influence factors of ethanol concentration, extraction temperature, extraction time, solid-liquid ratio, ultrasonic power on the extraction of tea saponin were studied in detail. Orthogonal experiment through the single factor experiment design, the experimental results show that the optimum operating conditions for the ethanol concentration of 80%, solid-liquid ratio 1: 40, extraction temperature 40°C, ultrasonic power of 300W, ultrasonic extraction time 45 minutes. Under the optimum conditions obtained tea saponin extract with ethanol- acetone precipitation method is further purified, tea saponin content increased from 52.1% to 93.6%. The result indicated that the purity of the tea saponin could be effectively improved through the ultrasonic assisted- precipitation combined method.

Optimization of Ultrasound Extraction of Nitrite Nitrogen in Sediment by Response Surface Methodology

2014 ◽  
Vol 955-959 ◽  
pp. 1466-1470 ◽  
Author(s):  
Dan Liu ◽  
Fang Yu ◽  
Wei Tan ◽  
Gui Zhen Li ◽  
Min Yang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Response Surface ◽  
Process Condition ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Liquid Ratio ◽  
Ultrasound Extraction ◽  
Nitrite Nitrogen ◽  
Solid Liquid

Response surface methodology based on single factors was used to optimize the process condition for extraction of nitrite nitrogen in sediment . The results indicated that the extraction amount of nitrite nitrogen was 9.5μg /g under the optimum condition of extraction time of 44min, ultrasonic power of 280W, solid-liquid ratio of 1:19,which was closed to the predicated yield of 9.3μg/g. The process can be used for the extraction of nitrite nitrogen in sediments.

scholarly journals Optimization of Malted Sorghum Protein Extraction by Response Surface Methodology

2013 ◽  
Vol 6 ◽  
pp. 53-58
Author(s):  
Irakoze Pierre Claver ◽  
Sindayigaya Ernest
Keyword(s):  
Protein Extraction ◽  
Extraction Process ◽  
Protein Yield ◽  
Extraction Time ◽  
Coefficient Of Determination ◽  
Ultrasonic Power ◽  
Order Model ◽  
Independent Variables ◽  
Sorghum Flour

Four independent variables viz. ultrasonic power, pH, extraction time and solvent/meal ratio were selected. The extraction process was evaluated by a selected response like protein yield and the second-order model obtained revealed 96.7% of coefficient of determination. Selected response, which evaluated the extraction process, was protein yield and the secondorder model obtained for protein yield revealed a coefficient of determination of 96.7%. The optimal extraction conditions for protein were determined as follows; Ultrasonic power, pH, extraction time and solvent/meal ratio were 400W, 8.0, 40min, and 20:1 (v/w) respectively. Protein yield was primarily affected by Ultrasonic power, pH and solvent/meal ratio. These conditions resulted in protein yield of 5.43g of soluble protein from extract/100 g malted sorghum flour, which was agreed closely with the predicted value 5.36%. The adequacy of the model was confirmed by extracting the protein under optimum values using the model. These results may help in designing the process of optimal protein extraction from malted sorghum flour. J. Food Sci. Technol. Nepal, Vol. 6 (53-58), 2010 DOI: http://dx.doi.org/10.3126/jfstn.v6i0.8260

Ultrasonic-Assisted Extraction and Antibacterial Properties of Polyphenols of Sweet Corncob

2014 ◽  
Vol 1028 ◽  
pp. 374-379
Author(s):  
Xin Wang ◽  
Li Ping Xu
Keyword(s):  
Volume Fraction ◽  
Orthogonal Experiment ◽  
Antibacterial Properties ◽  
Methanol Concentration ◽  
Extraction Time ◽  
Liquid Ratio ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Solid Liquid ◽  
Ultrasound Power

This paper reported the extraction conditions of polyphenols compounds by ultrasonic wave and its bacteriostasis from sweet corncob. The yield of Polyphenol was selected as the index which determined by the Folin-Ciocalteu assay, single factor experiment and orthogonal experiment of ultrasound power, the extraction time, the methanol concentration (volume fraction) and solid-liquid ratio had been taken out. The result of orthogonal experiment showed that the best ultrasound power, the extraction time, the methanol concentration (volume fraction) and solid-liquid ratio were 520W, 20min, 1:10 (g/mL) and 80%, respectively. Calculation showed that the yield of Polyphenols up to 2.85%.The extract of Polyphenols from sweet corncob showed that it had bacteriostasis to EscherichiaColi,BacillusSubtilisandStaphylococcusAlbus.

Study on Extraction of Dioscin from D.zingiberensis

2011 ◽  
Vol 189-193 ◽  
pp. 863-866
Author(s):  
Xiang Li ◽  
Jian Zhong Ma ◽  
Jing Xia
Keyword(s):  
Volume Fraction ◽  
Extraction Yield ◽  
Extraction Rate ◽  
Extraction Time ◽  
Ultrasonic Frequency ◽  
Liquid Ratio ◽  
Ethanol Extraction ◽  
Ultrasonic Assisted ◽  
Solid Liquid

The effects of extraction conditions on Extraction of Dioscin from D.zingiberensis was studied in this paper. It was shown that when the ultrasonic frequency was 35.74KHz, solid-liquid ratio(D.zingiberensis:ethanol)was 1:10, ethanol volume fraction was 75%, and extracted for 30 minutes and for 3 times, the extraction rate of dioscin from D.zingiberensis reached the highest. The method of ultrasonic-assisted ethanol extraction can not only cut down extraction time, but also increase extraction yield.

Ultrasonic Assisted Extraction Process Optimization of Malabar Spinach Seed Oil and GC-MS Analysis

2015 ◽  
Vol 713-715 ◽  
pp. 2997-3000
Author(s):  
Li Na Liu
Keyword(s):  
Extraction Time ◽  
Optimum Process ◽  
Process Conditions ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Power Extraction ◽  
Ms Analysis ◽  
Assisted Extraction ◽  
Solid Liquid

This paper selects petroleum ether as an extraction solvent, use ultrasonic to assisted extraction of Malabar spinach oil, research of extraction temperature, ultrasonic power extraction, extraction time, solid-liquid ratio on Malabar spinach oil extraction rate by orthogonal, extract rate was used as the main criterion to determine the optimum process conditions. The results showed that: the optimum conditions was ultrasonic power 135W, extraction temperature 40 °C, extraction time 25min, solid-liquid ratio 1:10. Identified 19 kinds of main ingredientby GC-MS analysis.

ULTRASONIC-ASSISTED EXTRACTION OF OIL FROM CALOPHYLLUM INOPHYLLUM SEEDS: OPTIMIZATION OF PROCESS PARAMETERS

2016 ◽  
Vol 78 (10) ◽  
Author(s):  
Faiznur Mohd Fuad ◽  
Mashitah Mat Don
Keyword(s):  
Solvent Extraction ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Calophyllum Inophyllum ◽  
Power Extraction ◽  
Optimization Of Process Parameters ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Ultrasonic Assisted Extraction

Calophyllum inophyllum (C. inophyllum) is a plant known as “Penaga Laut” in Malaysia. Its seeds contain valuable oil that serve as a potential alternative sources for vegetable oil. In this study, C. inophyllum seeds oil was extracted using an ultrasonic-assisted extraction (UAE) technique. The optimization of extraction parameters namely different types of solvent, extraction time, ultrasonic power, extraction temperature and liquid to solid (L/S) ratio were performed using one-factor-at-a-time (OFAT) method. The optimum extraction conditions obtained were n-hexane as a solvent, extraction time 20 min, ultrasonic power 210 W, extraction temperature 40°C and L/S ratio 20 ml/g, with an oil yield 55.44 ± 0.53 %.

Ultrasonic Extraction Chuanxiong Polysaccharide of Influencing Factors

2012 ◽  
Vol 550-553 ◽  
pp. 1071-1075
Author(s):  
Yong Guang Bi ◽  
Chun Chun Liu
Keyword(s):  
Influencing Factors ◽  
Extraction Process ◽  
Extraction Rate ◽  
Ultrasonic Extraction ◽  
Ultrasonic Power ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Ultrasonic Time ◽  
Solid Liquid

In order to study the the Chuanxiong polysaccharide ultrasonic extraction process, examine the ultrasonic time, ultrasonic power, solid-liquid ratio and extraction temperature on the Chuanxiong polysaccharide extract rate. The results show that the role of time at 30min Polysaccharide better, the extraction rate is 26.63%. when the ultrasonic power is 320W, Polysaccharide extraction rate is 26.15%.When the solid-liquid ratio is 1:20, polysaccharide extraction rate is 27.73%. When the extraction temperature is 60°C, the polysaccharide extraction rate is 27.73%.

Optimization of Pectin Extraction from Ramie by Orthogonal Methodology and Its Partial Physicochemical Properties

2019 ◽  
Vol 11 (11) ◽  
pp. 1607-1611
Author(s):  
Lifeng Cheng ◽  
Shengwen Duan ◽  
Xiangyuan Feng ◽  
Ke Zheng ◽  
Qi Yang ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Orthogonal Experiment ◽  
Ammonium Oxalate ◽  
Scientific Basis ◽  
Extraction Time ◽  
Single Element ◽  
Extraction Temperature ◽  
Optimal Conditions ◽  
Liquid Ratio ◽  
Solid Liquid

Pectin was extracted from Ramie [Boehmeria nivea (L.) Gaud] through ammonium oxalate extraction method. The influences of single factors (ammonium oxalate concentration, extraction time, solid–liquid ratio, and reaction temperature) on the yield of ramie pectin were studied. Orthogonal experiment was then performed to optimize the combination of the given conditions using pectin yield as the measuring indicator. Finally, the partial physicochemical properties of ramie pectin extracted in the optimal conditions were analyzed. Results showed that the optimal conditions for single-element experiment were 3.5 h (extraction time), 1:25 (solid–liquid ratio), 0.85% (concentration of ammonium oxalate) and 95 °C (extraction temperature), and that the optimal combination conditions for orthogonal experiment was 4 h, 1:30, 0.95% and 90 °C. The ramie pectin yield extracted in optimal conditions reached 15.81%, and its protein content, galacturonic acid content, and esterification degree were 0.659 mg/g, 49.92%, and 53.11%, respectively. Therefore, the proposed method was successfully applied to obtain ramie pectin and may provide scientific basis for improving the multi-utilization value of ramie.

Optimization of Extracting Condition of Active Components in Perilla by Ultrasonic

2014 ◽  
Vol 1033-1034 ◽  
pp. 713-716
Author(s):  
Dan Huang ◽  
You Qing Liu ◽  
Yuan Liang ◽  
Xiang Mao
Keyword(s):  
Rosmarinic Acid ◽  
Extraction Rate ◽  
Ultrasonic Power ◽  
Single Factor ◽  
Liquid Ratio ◽  
Active Components ◽  
Extraction Technology ◽  
Extraction Solvent ◽  
Solid Liquid ◽  
Temperature Time

Using ultrasonic-extraction technology, the condition of extracting the active compounds-flavonoids and rosmarinic acid ofPerillawas studied. The effects of four main factors include ultrasonic power, temperature, time and solid-liquid ratio on the extraction-rate of the flavonoids and the rosmarinic acid ofPerillawere reviewed by single factor experiments,and the flavonoids and the rosmarinic acid was determined by NaNO2-Al (NO3)3-NaOH method and FeSO4method. On the base of single factor experiments, the extraction technique was optimized in the orthogonal test. The result showed that temperature was the most important factor in the influencing factors on the extraction-rate. The subsequence of importance was temperature > time > solid-liquid ratio > ultrasonic. The optimal extraction conditions were: extraction solvent 3%borax, solid-liquid ratio 1:50(w/v), ultrasonic power 300W, time 90min , temperature 75°C. Under the optimum technological conditions, 34.64mg/g of flavonoids and rosmarinic acid fromPerillacould be obtained.

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