scholarly journals Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptáme and growth of pepper and cucumber

2011 ◽  
Vol 52 (No. 3) ◽  
pp. 130-137 ◽  
Author(s):  
H.S. Han ◽  
Supanjani ◽  
K.D. Lee
Keyword(s):  
Plant Growth ◽  
Bacillus Megaterium ◽  
Direct Application ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Bacillus Mucilaginosus ◽  
Bacterial Inoculum ◽  
Phosphate Solubilizing ◽  
Potassium Solubilizing Bacteria ◽  
Potential Use

Biofertilizers have been used as sources to improve plant nutrients in sustainable agriculture. Experiments were conducted to evaluate the potential of phosphate solubilizing bacteria (PSB) Bacillus megaterium var. phosphaticum and potassium solubilizing bacteria (KSB) Bacillus mucilaginosus inoculated in nutrient limited soil planted with pepper and cucumber. Results showed that rock P and K applied either singly or in combination did not significantly enhance soil availability of P and K, indicating their unsuitability for direct application. PSB was a more potent P-solubilizer than KSB, and co-inoculation of PSB and KSB resulted in consistently higher P and K availability than in the control without bacterial inoculum and without rock material fertilizer. Integrated rock P with inoculation of PSB increased the availability of P and K in soil, the uptake of N, P and K by shoot and root, and the growth of pepper and cucumber. Similar but less pronounced results were obtained when rock K and KSB were added concomitantly. Combined together, rock materials and both bacterial strains consistently increased further mineral availability, uptake and plant growth of pepper and cucumber, suggesting its potential use as fertilizer.

scholarly journals Selected Rhizosphere Bacteria Help Tomato Plants Cope with Combined Phosphorus and Salt Stresses

2020 ◽  
Vol 8 (11) ◽  
pp. 1844
Author(s):  
Gylaine Vanissa Tchuisseu Tchakounté ◽  
Beatrice Berger ◽  
Sascha Patz ◽  
Matthias Becker ◽  
Henri Fankem ◽  
...  
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Tomato Plant ◽  
Stress Conditions ◽  
Soil Conditions ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Salt Affected Soils ◽  
Phosphate Solubilizing

Plants are often challenged by multiple abiotic stresses simultaneously. The inoculation of beneficial bacteria is known to enhance plant growth under these stresses, such as phosphorus starvation or salt stress. Here, for the first time, we assessed the efficiency of selected beneficial bacterial strains in improving tomato plant growth to better cope with double stresses in salty and P-deficient soil conditions. Six strains of Arthrobacter and Bacillus with different reservoirs of plant growth-promoting traits were tested in vitro for their abilities to tolerate 2–16% (w/v) NaCl concentrations, and shown to retain their motility and phosphate-solubilizing capacity under salt stress conditions. Whether these selected bacteria promote tomato plant growth under combined P and salt stresses was investigated in greenhouse experiments. Bacterial isolates from Cameroonian soils mobilized P from different phosphate sources in shaking culture under both non-saline and saline conditions. They also enhanced plant growth in P-deficient and salt-affected soils by 47–115%, and their PGP effect was even increased in higher salt stress conditions. The results provide valuable information for prospective production of effective bio-fertilizers based on the combined application of local rock phosphate and halotolerant phosphate-solubilizing bacteria. This constitutes a promising strategy to improve plant growth in P-deficient and salt-affected soils.

scholarly journals Profiling of Plant Growth-Promoting Metabolites by Phosphate-Solubilizing Bacteria in Maize Rhizosphere

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1071
Author(s):  
Minchong Shen ◽  
Jiangang Li ◽  
Yuanhua Dong ◽  
Hong Liu ◽  
Junwei Peng ◽  
...  
Keyword(s):  
Plant Growth ◽  
Microbial Control ◽  
Agricultural Practices ◽  
Phosphate Solubilizing Bacteria ◽  
Mean Values ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Phosphate Solubilizing ◽  
Microbial Treatments

Microbial treatment has recently been attracting attention as a sustainable agricultural strategy addressing the current problems caused by unreasonable agricultural practices. However, the mechanism through which microbial inoculants promote plant growth is not well understood. In this study, two phosphate-solubilizing bacteria (PSB) were screened, and their growth-promoting abilities were explored. At day 7 (D7), the lengths of the root and sprout with three microbial treatments, M16, M44, and the combination of M16 and M44 (Com), were significantly greater than those with the non-microbial control, with mean values of 9.08 and 4.73, 7.15 and 4.83, and 13.98 and 5.68 cm, respectively. At day 14 (D14), M16, M44, and Com significantly increased not only the length of the root and sprout but also the underground and aboveground biomass. Differential metabolites were identified, and various amino acids, amino acid derivatives, and other plant growth-regulating molecules were significantly enhanced by the three microbial treatments. The profiling of key metabolites associated with plant growth in different microbial treatments showed consistent results with their performances in the germination experiment, which revealed the metabolic mechanism of plant growth-promoting processes mediated by screened PSB. This study provides a theoretical basis for the application of PSB in sustainable agriculture.

scholarly journals Isolation and identification of phosphate solubilizing bacteria and evaluate its effect on of Mung bean (Vigna radita [L.] R.Wilczek) growth

2019 ◽  
Vol 60 (5) ◽  
pp. 985-995
Author(s):  
Yusur Ramzi ◽  
Hutaf A. A. Alsalim
Keyword(s):  
Plant Growth ◽  
Bacillus Cereus ◽  
Mung Bean ◽  
Phosphate Solubilization ◽  
Growth Promotion ◽  
Hydrolytic Enzymes ◽  
Phosphate Solubilizing Bacteria ◽  
Biochemical Tests ◽  
Isolation And Identification ◽  
Phosphate Solubilizing

Sixteen soil samples were collected from wheat, barley and yellow corn rhizosphere in Abu-Ghraib, Aqraqof, Latifieh,Tarmiah, Jadriya and  of Agriculture in Baghdad university/ Baghdad city. The results found nine phosphate solubilizing bacteria (PSB) isolates (Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, Y9), formed clear zones on National Botanical Research Institute's (NBRIP) agar. The solubility index (SI) of PSB isolates ranged from 2.00 to 3.66. Y4 have the highest SI (3.66) followed by Y3 and Y6 (3.33). Phosphate solubilization abilities varying from (20.10-39.00 μg.ml-1), Y4 was the highest (39.00 μg.ml-1) followed by Y3 (37.00μg.ml-1). The results of hydrolytic enzymes production showed that almost all nine isolates are able to produce protease and pectinase, while Y1 and Y2 showed negative results in cellulase production. Maximum ability for hydrogen cyanide (HCN) and indole acetic acid (IAA) production were showed byY3 and Y4 isolates. The isolate Y4 was found to be the most efficient isolate, so it was selected identified as Bacillus cereus using biochemical tests confirmed by VITEC 2 compact system. The results of High performance liquid chromatography (HPLC) revealed that Bacillus cereus produce oxalic acid (2.996), citric acid (9.117) and malic acid (3.734). Bacillus cereus (Y4) enhanced the growth of mung bean plants. A significant increase in branches number (12.33), plant length (83.0cm), fresh weight (27.25 g) and dry weight (1.427g) were obtained compared with control treatments. The main objective of this study is to isolate PSB and evaluate their roles in plant growth promotion. The results showed the high phosphate solubilization efficiency of PSB isolates and the identified isolates was found to be good enough for plant growth promoting.

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from agricultural soils for a potential use in cultivating Capsicum frutescens

2020 ◽  
Vol 10 (2) ◽  
pp. 5161-5173
Keyword(s):  
Plant Growth ◽  
Agricultural Soils ◽  
Pot Experiment ◽  
Available Phosphorus ◽  
Phosphate Solubilizing Bacteria ◽  
Capsicum Frutescens ◽  
Isolation And Characterization ◽  
Insoluble Form ◽  
Phosphate Solubilizing

Phosphorus (P) is one of the essential macronutrients needed for the plant growth, other than nitrogen and potassium. Most phosphorus remains as insoluble form in soils and the plants only can uptake the phosphorus nutrient in soluble forms. Phosphate solubilizing bacteria (PSB) dissolves the phosphorus and make it available for plants. In this study, Soil samples were collected and screened for PSB on PK medium. PSB colonies with the highest phosphate solubilization ability were chosen and used for studying its rhizosphere effect on Capsicum frutescens by pot experiment.. It was evidenced that selected PSB strain could solubilize phosphate in PK medium and modified PK broth. Besides, it provided available phosphorus for plants and enhanced the plant growth in pot experiment.

scholarly journals Isolation and Characterization of Phosphate-Solubilizing Bacteria from Mushroom Residues and their Effect on Tomato Plant Growth Promotion

2017 ◽  
Vol 66 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Jian Zhang ◽  
Peng Cheng Wang ◽  
Ling Fang ◽  
Qi-An Zhang ◽  
Cong Sheng Yan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Wood Flour ◽  
Dry Weight ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Phosphate Solubilizing Bacteria ◽  
Sequence Comparisons ◽  
Isolation And Characterization ◽  
Phosphate Solubilizing

Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Highly efficient phosphate-solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. In this study, 13 isolates were obtained from waste mushroom residues, which were composed of cotton seed hulls, corn cob, biogas residues, and wood flour. NBRIP solid medium was used for isolation according to the dissolved phosphorus halo. Eight isolates produced indole acetic acid (61.5%), and six isolates produced siderophores (46.2%). Three highest phosphate-dissolving bacterial isolates, namely, M01, M04, and M11, were evaluated for their beneficial effects on the early growth of tomato plants (Solanum lycopersicum L. Wanza 15). Strains M01, M04, and M11 significantly increased the shoot dry weight by 30.5%, 32.6%, and 26.2%, and root dry weight by 27.1%, 33.1%, and 25.6%, respectively. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, strains M01 and M04 belonged to the genus Acinetobacter, and strain M11 belonged to the genus Ochrobactrum. The findings suggest that waste mushroom residues are a potential resource of plant growth-promoting bacteria exhibiting satisfactory phosphate-solubilizing for sustainable agriculture.

scholarly journals Diseño de un medio para la producción de un co-cultivo de bacterias fosfato solubilizadoras con actividad fosfatasa

2012 ◽  
Vol 17 (1) ◽  
pp. 43 ◽  
Author(s):  
Jimena Paola Angulo-Cortés ◽  
Anamaría García-Díaz ◽  
Aura Marina Pedroza ◽  
María Mercedes Martínez-Salgado ◽  
Viviana Gutiérrez-Romero
Keyword(s):  
Phosphatase Activity ◽  
Microbial Growth ◽  
Culture Media ◽  
Biomass Concentration ◽  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Bacterial Strains ◽  
Box Behnken Design ◽  
Phosphate Solubilizing ◽  
Complex Culture

<strong>Objective</strong>. To design a complex culture media for the production of biomass and acid phosphatases from phosphate-solubilizing bacteria isolated from soil. <strong>Materials</strong> <strong>and methods</strong>. Phosphate-solubilizing bacteria were isolated from oil palm crop soil samples and selected on SMRS1 agar, which were then assessed with antagonism tests to verify their aptitude to form a co-culture. A Box-Behnken experimental design was applied to<br />evaluate the effect of each one of the culture media components on the production of biomass and phosphatase enzymes at a laboratory scale. Finally, microbial growth and enzyme production curves were carried out in order to determine their production times. <strong>Results</strong>. Five phosphate-solubilizing bacterial strains were isolated and three of them were selected based on their solubilization indices.These Gram negative strains with bacillus morphology were identified as A, B and C; their solubilization indices were 2.03, 2.12, and 2.83, respectively. According to the ANOVA analyses for the Box-Behnken design, the only factor which had a significant effect on the phosphatase activity (p&lt;0.01) was hydrolyzed yeast, and the formulation that generated the highest biomass concentration and phosphatase activity (p&lt;0.01) contained 10, 15 and 2.5 gL-1 of phosphoric rock, sucrose and hydrolyzed yeast, respectively. After 24 hours of incubation at 100 rpm, the highest values of biomass and phosphatase activity were obtained: 11.8 logarithmic units of CFU and 12.9 phosphatase units. <strong>Conclusion</strong>. We determined that the culture media based on phosphoric rock 10 gL-1, hydrolyzed yeast 2.5 gL-1 and commercial sucrose 15 gL-1 was ideal for the production of biomass and phosphatases by the strains evaluated; likewise, we proved that the hydrolyzed yeast was the only factor significantly influential for the production of phosphatases.<br /><br /><strong>Key words</strong>: bio-inoculants, phosphate solubilizing microorganisms, phosphatase activity, Box Behnken design.

scholarly journals Isolation of Phosphate Solubilizing Bacteria and their Use for Plant Growth Promotion in Tomato Seedling and Plant

2018 ◽  
Vol 13 (2) ◽  
pp. 61-70
Author(s):  
Rajiv Pathak ◽  
Vipassana Paudel ◽  
Anupama Shrestha ◽  
Janardan Lamichhane ◽  
Dhurva. P. Gauchan
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
In Vitro Study ◽  
Dry Weight ◽  
Greenhouse Experiment ◽  
Phosphate Solubilizing Bacteria ◽  
Tomato Seedling ◽  
Phosphate Solubilizing ◽  
Phosphorous Compounds

Phosphorous (P) is an essential macronutrient and most soils contain high levels of P. However, its availability to plant is limited by rapid immobilization of phosphorous compounds to insoluble forms and hence plant available forms of P in soils are found in low amounts. Phosphate solubilizing bacteria provide an eco-friendly alternative to convert insoluble phosphates into plant available forms. In the present study, three phosphate solubilizing bacterial isolates (PB-1, PB-4 and VC-01) with visually significant phosphate solubilizing abilities were isolated from tomato rhizosphere soil. In-vitro study in pikovskaya’s agar revealed that isolate PB-1 had the highest phosphate solubilizing ability with a phosphate solubilizing index of 2.08±0.07 followed by isolate VC-01 (1.31±0.09) and PB-4 (1.24±0.08). Isolates were used as bacterial inoculum to assess their ability to promote tomato (Lycopersicon esculentum var. Srijana) seedling and plant growth in in-vitro and greenhouse experiment respectively. Isolate PB-4 showed best growth promotion in seedling assay whereas isolate PB-1 and VC-01 also promoted seedling growth compared to control. In greenhouse experiment however, isolates VC-01 and PB-1 significantly enhanced all parameters (shoot length, root length, shoot and root dry weight) compared to uninoculated control whereas isolate PB-4 had a positive effect on all parameters except root length.Kathmandu University Journal of Science, Engineering and TechnologyVol. 13, No. 2, 2017, page: 61-70

scholarly journals Integrated Effects of Co-Inoculation with Phosphate-Solubilizing Bacteria and N2-Fixing Bacteria on Microbial Population and Soil Amendment Under C Deficiency

2019 ◽  
Vol 16 (13) ◽  
pp. 2442 ◽  
Author(s):  
Zhikang Wang ◽  
Ziyun Chen ◽  
Xiangxiang Fu
Keyword(s):  
Plant Growth ◽  
Soil Properties ◽  
Soil Amendment ◽  
Growth Promotion ◽  
Synergistic Effects ◽  
Available P ◽  
P Availability ◽  
Phosphate Solubilizing Bacteria ◽  
Unsterilized Soil ◽  
Phosphate Solubilizing

The inoculation of beneficial microorganisms to improve plant growth and soil properties is a promising strategy in the soil amendment. However, the effects of co-inoculation with phosphate-solubilizing bacteria (PSB) and N2-fixing bacteria (NFB) on the soil properties of typical C-deficient soil remain unclear. Based on a controlled experiment and a pot experiment, we examined the effects of PSB (M: Bacillus megaterium and F: Pseudomonas fluorescens), NFB (C: Azotobacter chroococcum and B: Azospirillum brasilence), and combined PSB and NFB treatments on C, N, P availability, and enzyme activities in sterilized soil, as well as the growth of Cyclocarya Paliurus seedlings grow in unsterilized soil. During a 60-day culture, prominent increases in soil inorganic N and available P contents were detected after bacteria additions. Three patterns were observed for different additions according to the dynamic bacterial growth. Synergistic effects between NFB and PSB were obvious, co-inoculations with NFB enhanced the accumulation of available P. However, decreases in soil available P and N were observed on the 60th day, which was induced by the decreases in bacterial quantities under C deficiency. Besides, co-inoculations with PSB and NFB resulted in greater performance in plant growth promotion. Aimed at amending soil with a C supply shortage, combined PSB and NFB treatments are more appropriate for practical fertilization at intervals of 30–45 days. The results demonstrate that co-inoculations could have synergistic interactions during culture and application, which may help with understanding the possible mechanism of soil amendment driven by microorganisms under C deficiency, thereby providing an alternative option for amending such soil.

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