greenhouse experiment
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2022 ◽  
Vol 82 ◽  
Author(s):  
D. D. da Silva ◽  
S. M. Mendes ◽  
D. F. Parreira ◽  
R. C. Pacheco ◽  
R. C. Marucci ◽  
...  

Abstract We report the discovery that the earwig predator Doru luteipes (Scudder, 1876) (Dermaptera: Forficulidae) feed on Puccinia polysora Underw uredospore, the causal agent of Southern Rust of Corn (SRC), which is a primary disease affecting the maize crop in Brazil. We performed experiments in laboratory and greenhouse to test the effect of D. luteipes (1st/2nd and 3rd/4th instars, and adults) fungivory on the P. polysora uredospore concentration. All trials showed a significant reduction of the initial concentration of uredospore. There was a reduction in uredospore concentration with increase in number of D. luteipes feeding on them. We also tested the uredospore consumption by quantifying its percentage in the feces of D. luteipes. Nymphs of the 2nd, 4th instar and adults fed 88%, 85%, and 83.8% of the uredospore, respectively. For nymphs of the 3rd instar, the percentage of uredospore consumption (75.6%) was statistically significant compared with the other groups. In greenhouse experiment, at twenty-eight days after plant inoculation with 9.9 x 104 uredospores, the percentage of uredospore consumption was 81.7%. Our results confirmed the fungivory of D. luteipes on P. polysora uredospore. This is the first report of D. luteipes fungivory, which may play an important role in the biological control of P. polysora in corn.


Agriculture ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1264
Author(s):  
Urszula Wachowska ◽  
Edyta Kwiatkowska ◽  
Wioletta Pluskota

Background: Sida hermaphrodita (Virginia fanpetals) was introduced to Poland nearly 70 years ago as a potential fodder plant, and it is gaining importance as an energy crop. Alternaria alternata transmitted by seeds may exert a negative effect on the health of Virginia fanpetals plants. Methods: The virulence of the A. alternata pathogen, isolated from Virginia fanpetals seeds, was tested on detached leaves of Virginia fanpetals plants. The isolates were identified as A. alternata based on partial sequence analysis of Alta1, TEF1a and gdp genes and the ITS 1–5.8SrDNA–ITS 2 region. Pathogen transmission from seeds to seedlings and the influence of seed dressing with a suspension of Aureobasidium pullulans on seedling health were analyzed in a greenhouse experiment. Results: Three of the nine analyzed A. alternata isolates were highly pathogenic for S. hermaphrodita. The initial symptoms of leaf infection were small, round dark brown or black spots which grew into larger dark brown spots surrounded by a chlorotic halo. Alternaria alternata was re-isolated from inoculated plants and was identified as the causal agent of Alternaria leaf spot disease. In the greenhouse experiment, S. hermaphrodita seeds dressed with a suspension of A. pullulans and inoculated with A. alternata produced a higher number of seedlings with a higher health status than non-dressed seeds. Conclusions: The study demonstrated that A. alternata is transmitted from infected S. hermaphrodita seeds to developing plants and biological control limits this phenomenon.


2021 ◽  
Vol 937 (2) ◽  
pp. 022131
Author(s):  
N Bagnavets ◽  
A Zhevnerov ◽  
M Grigoryeva ◽  
T Pshenichkina

Abstract Phosphate fertilizers are widely used for growing crops. To obtain phosphoric fertilizers, phosphoric acid purified using organic solvents, one of which is 100% tributyl phosphate, is used. The authors of the paper have chosen this solvent to obtain purified phosphoric acid. Purification from impurities included the stages of extraction and reextraction. On the basis of the re-extract and chemically pure phosphoric acid, a phosphoric fertilizer magnesium-ammonium phosphate (MgNH4PO4) was synthesized and used in the greenhouse experiment. The greenhouse experiment consisted in growing a tomato crop included three options for fertilization. A comparative analysis of the stimulating effect of magnesium-ammonium phosphate, synthesized with the use of phosphoric acid of varying degrees of purification, on the yield of tomato culture has been carried out.The paper proposes a blister-colorimetric version of the determination of phosphate ions in a blister cell containing a mixture of dry reagents in the form of a bulk. The optimal conditions for carrying out colorimetry have been selected. The proposed method makes it possible to semi-quantitatively determine phosphorus in various objects without preparing reagent solutions, using a bulk packed in an ampoule or blister, and can be used to control phosphorus consumption by plants, which, in turn, allows regulating the introduction of nutrients in the form of dressings.


Author(s):  
Prerna Bungla ◽  
S. P. Pachauri ◽  
P. C. Srivastava ◽  
Anand Pathak ◽  
Anil Kumar Shukla

A greenhouse experiment was conducted to study the effect of effect of varying levels of boron and sulphur on yield and nutrient uptake of linseed (Linum usitatissimum L.) grown in a Mollisol. The experiment was conducted under factorial completely randomized design with three replications and consisted of six levels of each of boron (0, 0.5, 1.0, 1.5, 2.0 mg kg−1soil and two foliar sprays of 0.2% boron) and sulphur (0, 10, 20, 30, 40 and 60 mg kg−1soil). The different doses of sulphur and boron and their interaction had significant effect on seed and stover yields, B and S concentrations and uptake by linseed crop indicating a differential requirement of boron and sulphur by the crop.  The highest seed yield was recorded under the combined application of 2 mg B kg−1 soil and 60 mg S kg−1 soil.


Author(s):  
Xorla Kanfra ◽  
Ahmed Elhady ◽  
Hendrik Thiem ◽  
Sven Pleger ◽  
Markus Höfer ◽  
...  

AbstractPhytonematodes cause severe yield losses in horticulture, partly because they are difficult to manage. Compact, energy-efficient generators that electrochemically produce ozonated water by utilizing diamond-coated electrodes have become available. In this study, the application of on-site generated ozonated water to inactivate soil nematodes and to mitigate nematode-mediated apple replant disease was tested. Pratylenchus penetrans was highly susceptible to dissolved ozone (LC50 0.6 mg L−1). In one greenhouse experiment, treatment of P. penetrans in soil with ozonated water (0.27 mg ozone L−1 soil) reduced subsequent invasion of the nematodes into roots by 60%. Growth of apple saplings in soil that was affected by apple replant disease (ARD) was significantly improved following a treatment with 1/10 volume ozonated water compared to the control. In a second greenhouse experiment, one-time drenching of ARD soil with ozonated water was followed by improved growth of apple plants similar to that in autoclaved soil. A second application of ozonated water did not further improve plant growth. The number of active nematodes in replanted soil that moved through a Baermann filter was significantly reduced by all tested concentrations of ozone (0.12–0.75 mg L−1 soil). A fraction of 19–36% of the nematodes survived and slightly recovered after four weeks. In conclusion, on-site generated ozonated water has potential to mitigate nematode problems in horticulture and to expand management options.


2021 ◽  
Author(s):  
Yongge Yuan ◽  
Huifei Jin ◽  
Junmin Li

Abstract Aims There is an increasing likelihood that invasive plants are again exposed to their co-evolved specialist herbivores in the non-native range. However, whether there is a latitudinal pattern associated with the resistance of an invasive plant to its co-evolved herbivores and how soil microbes affect resistance has been little explored. We hypothesized that the resistance of invasive Solidago canadensis to its co-evolved insect herbivore Corythucha marmorata could increase with latitude, and that local rhizosphere microbes could facilitate invasive plants to become resistant to their co-evolved herbivores. Methods We conducted a field survey and a greenhouse experiment to examine whether there was a latitudinal pattern in the abundance of C. marmorata and in the damage it caused to S. canadensis in China. We tested whether local rhizosphere microbes of invasive plants can promote the resistance of S. canadensis to C. marmorata herbivory. Important findings In the field survey, both density of C. marmorata and damage level of S. canadensis were positively correlated with latitude, and with S. canadensis plant growth, indicating a latitudinal pattern in the resistance of S. canadensis to C. marmorata. However, in the greenhouse experiment, S. canadensis from different latitudes did not suffer significantly from different levels of damage from C. marmorata. Additionally, the damage level of S. canadensis was lower when rhizosphere soil and rhizomes originated from field S. canadensis with same damage level than with different damage levels. This result indicates that local rhizosphere soil microbes promote the adaptation of S. canadensis to resistance of C. marmorata.


Horticulturae ◽  
2021 ◽  
Vol 7 (9) ◽  
pp. 263
Author(s):  
Joshua B. Tebow ◽  
Lauren L. Houston ◽  
Ryan W. Dickson

The objective of this study was to evaluate silicon (Si) foliar spray and substrate drench effects on plant growth and morphology for container-grown edible crops during greenhouse production, as well as resistance to plant wilting during post-production. In the first greenhouse experiment, basil received Si foliar sprays at 0, 50, 100, 200, and 400 mg∙L–1 Si. In the second greenhouse experiment, Si was applied as either a foliar spray (500 mg∙L–1 Si) or substrate drench (100 mg∙L–1 Si) with six edible crop species. Supplemental Si increased shoot Si levels but had minimal effects on plant growth and morphology, except for parsley, which resulted in distorted growth and phytotoxicity. In the first experiment, 200 and 400 mg∙L–1 Si foliar sprays increased plant resistance to wilt by 2.2 and 2.5 d, respectively; however, this was not observed in the second experiment. All species accumulated Si with the control (no Si) treatments, indicating trace amounts of Si were taken up from the substrate, fertilizer, spray surfactant, and irrigation water. Only cucumber was classified as a Si “accumulator” with a high capacity for Si uptake. Results emphasize the need to conduct preliminary trials with supplemental Si to avoid issues of phytotoxicity.


Agriculture ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 688
Author(s):  
Ana Pintar ◽  
Zlatko Svečnjak ◽  
Josip Lakić ◽  
Ivan Magdić ◽  
Dragojka Brzoja ◽  
...  

Variations in soil pH have been shown to affect mesotrione adsorption, which in turn, may have an impact on crop susceptibility. Therefore, a greenhouse experiment was conducted to evaluate the effect of simulated mesotrione residues on pea crop grown in the typical agricultural soil (gleysol) of north-western Croatia. The soil pH was manipulated to obtain neutral (pH 7.0) and acidic (pH 5.0) values. Simulated mesotrione residues were 1.1, 2.3, 4.5, 9.0, 18, 36 and 72 g a.i. ha−1. Crop visual injuries as well as reductions in chlorophyll fluorescence and aboveground dry biomass were higher at pH 7.0 than at pH 5.0. With increasing mesotrione residues, the reductions in chlorophyll fluorescence ranged from 38.8% to 89.7% at pH 5.0 and from 63.7% to 99.3% at pH 7.0. Compared to chlorophyll fluorescence, the reductions in dry biomass were smaller and ranged from 49.2% to 96.8% at pH 7.0 and from 32.0% to 82.6% at pH 5.0 for the mesotrione residues from 1.1 to 72 g a.i. ha−1. These results indicate that soil pH is an important factor determining the susceptibility of pea crop to simulated mesotrione residues.


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