scholarly journals Use of Detached Seedling Leaf Test to Evaluate Wheat Resistance to Septoria Tritici Blotch

2011 ◽  
Vol 41 (No. 3) ◽  
pp. 112-116 ◽  
Author(s):  
L. Věchet ◽  
M. Vojáčková

Two experimental sets of selected winter wheat cultivars, breeding lines, old Czech and Slovak landraces and wheat wild relatives were infected with three isolates (R-116, UH-105, BR-331) of Mycosphaerella graminicola (anamorph Septoria tritici) isolated in the Czech Republic. Groups of cultivars with different disease severity to all three isolates of the pathogen were found. Differences between old Czech and Slovak landraces and wild wheat relatives were larger than between modern wheat cultivars and breeding materials. In experiment one the isolate BR-331 differed significantly from the other two isolates in virulence to the tested modern wheat cultivars and breeding lines. The method is considered as suitable for preliminary screening in wheat resistance breeding or phytopathological testing and as a complement to field tests.    

2013 ◽  
Vol 50 (1-2) ◽  
pp. 11-26
Author(s):  
Czesław Zamorski ◽  
Bogdan Nowicki ◽  
Ewa Mirzwa-Mróz

The aim of the work was to study an occurrence and intensity of septoria tritici blotch on wheat in different regions of Poland as well as reaction of winter wheat cultivars and breeding lines to infection by <em>Mycosphaerella graminicola</em> (Fuckel) Schroeter (an. <em>Septoria tritici</em> ( Rob. ex Desm .) under field conditions. Observation conducted during 1994-1996 indicated on occurrence of septoria tritici blotch each year and on important infection degree of winter wheat by <em>Mycosphaerella gruminicola</em> in some regions of the country. The highest infection was noted in 1995 and the lowest in 1994. Infection degree for majority of cultivars was quite high. Some cultivars and lines were characterized by very high infection. Among them were the old (Kujawianka Więcławska and Wysokolitewka Sztywnosłoma) and the newest (Tercja) cultivars. On some old cultivars (Leszczyńska Wczesna and Żelazna) even no traces of infection were observed although favourable weather condition existance and the presence of neighbourhood diseased plants. Field observation of cultivars and breeding lines showed different reaction of tested wheat genotypes to <em>Mycosphaerella graminicola</em> infection.


2013 ◽  
Vol 55 (1) ◽  
pp. 233-246
Author(s):  
Ewa Mirzwa-Mróz ◽  
Czesław Zamorski

The response of Polish winter wheat genotypes to <i>M.graminicola</i> (preliminary experiments and cultivar collections) was observed in different regions of Poland. Observations were carried out in 1995-1999. The winter wheat genotypes showed a broad spectrum of reaction to this pathogen. Between 1997 and 1999 the highest degree of infection on winter wheat breeding lines was noted in Kończewice. During this time no genotypes free from infection were observed (preliminary breeding experiments). Cultivars with no symptoms of <i>Septoria tritici</i> blotch (Leszczyńska Wczesna and Żelazna) were found among old genotypes in Słupia Wielka only in earlier experiments (1995-1996). In the years 1997-1999 the winter wheat cultivars were classified into groups on the basis of their response to the pathogen. The degree of infection for the majority cultivars was quite high.


2012 ◽  
Vol 58 (1) ◽  
pp. 97-100
Author(s):  
Lubomir Věchet ◽  
Eliška Sychrová

Reaction of six winter wheat cultivars and lines (Vlasta, Šárka, Charger, 00ST022, SG-U8044C a SG-U2113B) to <i>Mycosphaerella graminicola</i> isolate BR-331 and UH-05 on leaf segments of the detached second seedling leaf of cultivars placed on water agar with bezimidazole in clear plastic box were tested. The isolate BR-331 produced high occurrence of the disease in the cultivar Šárka, middle occurrence (the percentage covered by lesions bearing pycnidia) in the cultivar Vlasta and SG-U8044C and low occurrence in the line 00ST0022. The isolate UH-105 produced high occurrence in the cultivar 00ST022 and middle occurrence in the cultivar Vlasta. The cultivar Charger was resistant. The cultivar Šárka was attacked at least. Results show on different virulence of <i>M. graminicola</i> isolates to wheat cultivars.


2001 ◽  
Vol 50 (3) ◽  
pp. 325-338 ◽  
Author(s):  
J. K. M. Brown ◽  
G. H. J. Kema ◽  
H.-R. Forrer ◽  
E. C. P. Verstappen ◽  
L. S. Arraiano ◽  
...  

2014 ◽  
Vol 50 (No. 3) ◽  
pp. 201-207 ◽  
Author(s):  
V. Dumalasová ◽  
L. Leišová-Svobodová ◽  
P. Bartoš

Winter wheat cultivars recently registered in the Czech Republic were tested in three-year field tests for resistance to common bunt. Seeds were inoculated with a mixture of local strains of Tilletia tritici and T. laevis. None of the cultivars displayed a higher level of resistance compared with the resistant checks. The mean percentage of bunted ears in the three test series including checks was 39%. Mean bunt infection in resistant and susceptible checks was 2% and 63%, respectively. In the European Tilletia cooperative test performed in Prague-Ruzyně, thirty-five winter wheat cultivars from six countries were tested during 2007&ndash;2013. The cultivars Bill, Nadro, Quebon, Samurai, Stava and Tommi exhibited infection levels below 10% in the respective years of the test. Additionally, 75 breeding lines from six countries were tested. Infection levels below 1% were recorded in 56% of the lines and 1&ndash;10% levels in 19% of the lines. A close relationship between the resistant cvs. Tommi and Globus was confirmed using SSR allelic markers.


2011 ◽  
Vol 123 (5) ◽  
pp. 741-754 ◽  
Author(s):  
Seyed Mahmod Tabib Ghaffary ◽  
Olivier Robert ◽  
Valerie Laurent ◽  
Philippe Lonnet ◽  
Eric Margalé ◽  
...  

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1075
Author(s):  
Mateusz Przyborowski ◽  
Sebastian Gasparis ◽  
Maciej Kała ◽  
Wacław Orczyk ◽  
Anna Nadolska-Orczyk

Wheat (Triticum aestivum L.) grain hardness is determined mainly by variations in puroindoline genes (Pina-D1 and Pinb-D1), which are located on the short arm of chromosome 5D. This trait has a direct effect on the technological properties of the flour and the final product quality. The objective of the study was to analyze the mutation frequency in both Pin genes and their influence on grain hardness in 118 modern bread wheat cultivars and breeding lines cultivated in Poland, and 80 landraces from Poland. The PCR products containing the Pin gene coding sequences were sequenced by the Sanger method. Based on detected the SNPs (single-nucleotide polymorphisms) we designed CAPS (cleaved amplified polymorphic sequence) markers for the fast screening of Pinb alleles in a large number of genotypes. All analyzed cultivars, breeding lines, and landraces possess the wild-type Pina-D1a allele. Allelic variation was observed within the Pinb gene. The most frequently occurring allele in modern wheat cultivars and breeding lines (over 50%) was Pinb-D1b. The contribution of the remaining alleles (Pinb-D1a, Pinb-D1c, and Pinb-D1d) was much less (approx. 15% each). In landraces, the most frequent allele was Pinb-D1a (over 70%), followed by Pinb-D1b (21% frequency). Pinb-D1c and Pinb-D1g were found in individual varieties. SKCS (single-kernel characterization system) analysis revealed that grain hardness was strictly connected with Pinb gene allelic variation in most tested cultivars. The mean grain hardness values were significantly greater in cultivars with mutant Pinb variants as compared to those with the wild-type Pinb-D1a allele. Based on grain hardness measured by SKCS, we classified the analyzed cultivars and lines into different classes according to a previously proposed classification system.


Sign in / Sign up

Export Citation Format

Share Document