scholarly journals The RAPD analysis of several cultivars of grapevine (Vitis viniferaL.) and their clones

2011 ◽  
Vol 31 (No. 4) ◽  
pp. 136-139 ◽  
Author(s):  
H. Vlastníková ◽  
K. Moravcová ◽  
M. Pidra
Keyword(s):  
Rapd Markers ◽  
Rapd Analysis ◽  
Pinot Noir ◽  
The Other ◽  
Banding Patterns ◽  
Rapd Profile ◽  
Other Hand ◽  
Rapd Pattern

Nine identification RAPD markers (Moravcová et al. 2003) were used to distinguish 24 clones and grapevine cultivars. No polymorphism was detected among all the tested clones of Chardonnay, Pinot gris and Zweigeltrebe from Polešovice. Pinot noir, Pinot gris, Pinot blanc and Pinot Meunier were indistinguishable within clones, they also showed the identical RAPD profile within cultivars (except discussed sample No. 26). On the other hand, Auxerrois as a relative to cultivars of Pinot group showed unique patterns and may be classified as a different cultivar. Some irregularities within the cultivars of Pinot family from Oblekovice were also found, several of them gave different results from those expected: Pinot blanc sample 26 has the RAPD profile typical of Chardonnay. A new abnormal RAPD pattern as a marker of typical Chardonnay and Pinot profiles was observed in two cases. While RAPD banding patterns could not distinguish between the known clones, they were useful for distinguishing between phenotypically similar cultivars and for assessing the origins of cultivars thought to have originated as sports.    

scholarly journals Comparative karyotype and RAPD analysis for characterizing three varieties of Lycopersicon esculentum Mill.

2013 ◽  
Vol 41 (2) ◽  
pp. 149-154 ◽  
Author(s):  
Sheikh Shamimul Alam ◽  
Elham Ishrat ◽  
Md Yahia Zaman ◽  
Md Ahashan Habib
Keyword(s):  
Lycopersicon Esculentum ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Karyotype Analysis ◽  
The Other ◽  
Rapd Fingerprinting ◽  
Lycopersicon Esculentum Mill ◽  
Other Hand ◽  
Agriculture Research Institute ◽  
Research Institute

Three tomato varieties (Lycopersicon esculentum Mill.) viz. BARI tomato-11, BARI tomato-2 and BARI tomato-3 released from Bangladesh Agriculture Research Institute (BARI) were characterized by karyotype analysis and RAPD markers. BARI tomato-11 and BARI tomato-2 were found to possess 2n = 24 metacentric chromosomes. On the other hand, 2n = 25 metacentric chromosomes were observed in BARI tomato-3 revealing a trisomic variety. The karyotype of the three varieties was very symmetric and each of the varieties showed specific and unique RAPD fingerprinting. Therefore, based on karyotype and RAPD analysis the three tomato varieties could be characterized. DOI: http://dx.doi.org/10.3329/bjb.v41i2.13439 Bangladesh J. Bot. 41(2): 149-154, 2012 (December)

scholarly journals Polymorphism and Discrimination Capacity of Randomly Amplified Polymorphic Markers in an Olive Germplasm Bank

2001 ◽  
Vol 126 (1) ◽  
pp. 64-71 ◽  
Author(s):  
A. Belaj ◽  
I. Trujillo ◽  
R. de la Rosa ◽  
L. Rallo ◽  
M.J. Giménez
Keyword(s):  
Gel Electrophoresis ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Geographic Origin ◽  
Group Method ◽  
Polymorphic Markers ◽  
Germplasm Bank ◽  
Banding Patterns ◽  
Olive Cultivars

Random amplified polymorphic DNA (RAPD) analysis was performed on the main Mediterranean cultivars of olive (Olea europaea L.) from the Germplasm Bank of the Centro de Investigación y Formación Agraria “Alameda del Obispo” in Cordoba, Spain. One hundred and ninety reproducible amplification fragments were identified using 46 random primers followed by agarose gel electrophoresis. Some 63.2% of the amplification products were polymorphic, with an average of 2.6 RAPD markers obtained for each primer. The combination of polymorphic markers resulted in 244 banding patterns. The high degree of polymorphism detected made identification of all the cultivars (51) possible by combining the RAPD banding patterns of just only four primers: OPA-01, OPK-08, OPX-01, and OPX-03. Cultivar-specific RAPD markers and banding patterns were also found. A dendrogram based on unweighted pair-group method cluster analysis was constructed using a similarity matrix derived from the RAPD amplification products generated by the 46 primers. Three major groups of cultivars could be distinguished by RAPD analysis: 1) cultivars from east and northeast Spain, 2) Turkish, Syrian, and Tunisian cultivars, and 3) the majority of common olive cultivars in Spain. The dendrogram thus showed a good correlation between the banding patterns of olive cultivars and their geographic origin. A higher level of polymorphism was observed when polyacrylamide gel electrophoresis was used to separate the amplification products. Thus, adequate use of RAPD technology offers a valuable tool to distinguish between olive cultivars.

scholarly journals Random amplified polymorphic DNA (RAPD) analysis of Ornithobacterium rhinotracheale strains isolated from chickens in Turkey

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 526-530 ◽  
Author(s):  
G. Ozbey ◽  
Ertas HB ◽  
A. Muz
Keyword(s):  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Random Primer ◽  
Serotype A ◽  
Dna Assay ◽  
Rapd Profile ◽  
Rapd Pattern ◽  
Serotype B ◽  
Dna Profiles ◽  
Ornithobacterium Rhinotracheale

Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT strains were found to have different RAPD profiles. In addition, the RAPD assay showed almost similar DNA profiles among the tested strains of the serotypes A, B, D and E. The strain of serotype C did give a different RAPD profile. Within strains of the same serotype (A), different profiles were found but the strain of serotype (B) had an identical profile as strains of serotype A. This study suggests that more genotypes of ORT strains are present within the same serotype and thus that no relationship exists between the RAPD pattern of ORT and their serotype.

Use of Randomly Amplified Polymorphic Dna Markers as a Tool to Study Variation in Lichen-Forming Fungi

1999 ◽  
Vol 31 (3) ◽  
pp. 257-267 ◽  
Author(s):  
G. J. Murtagh ◽  
P. S. Dyer ◽  
P. C. McClure ◽  
P. D. Crittenden
Keyword(s):  
Dna Polymerase ◽  
Dna Markers ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Pcr Amplification ◽  
Randomly Amplified Polymorphic Dna ◽  
Banding Patterns ◽  
Rapd Pcr ◽  
Key Features ◽  
Axenic Cultures

AbstractA protocol is described to enable the production of reliable genetic fingerprints of lichen-forming fungi using randomly amplified polymorphic DNA (RAPD) markers. Key features of the method are the use of mycobiont DNA extracted from axenic cultures by a phenol-chloroform procedure, and PCR amplification using DyNAzyme II DNA polymerase. RAPD-PCR fingerprints of Graphis scripta, G. elegans and Phacographis dendritica were successfully generated using this protocol and individual isolates could be identified on the basis of differences in banding patterns produced. DNA extracted from whole thalli of G. scripia was also subjected to RAPD-PCR but the fingerprints produced differed from those given by axenic cultures of the mycobiont. Therefore difficulties of interpretation may arise when whole thalli are used in RAPD analysis.

scholarly journals Using Randomly Amplified Polymorphic DNA (RAPD) Markers to Identify Annona Cultivars

1995 ◽  
Vol 120 (5) ◽  
pp. 726-729 ◽  
Author(s):  
C.M. Ronning ◽  
R.J. Schnell ◽  
S. Gazit
Keyword(s):  
Native American ◽  
Efficient Method ◽  
Interspecific Hybrids ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Randomly Amplified Polymorphic Dna ◽  
Banding Patterns ◽  
Polymorphic Loci ◽  
Edible Fruit ◽  
Custard Apple

The native American genus Annona contains many species that are cultivated for their edible fruit, including the custard apple (A. reticuluta L.), soursop (A. muricata L.), cherimoya (A. cherimola L.), sugar apple (A. squamosa L.), and interspecific hybrids, the atemoyas. RAPD analysis of A. cherimola. `Campa' and `Jete,' A. squamosa `Lessard,' and the atemoyas `Ubranitzki,' `Malali,' and `Kaspi' resulted in very distinctive patterns, indicating that RAPD markers, may be an efficient method of fingerprinting genotypes within and between Annona species. All 15 primers used generated repeatable, polymorphic patterns. An F1 population of `Jete' × `Lessard' was analyzed to determine the inheritance of the RAPD banding patterns. Fifty-two polymorphic loci were identified, which segregated in an expected Mendelian fashion.

scholarly journals Analysis of genetic relationships between populations of cashew (Anacardium occidentale L.) by using morphological characterisation and RAPD markers

2011 ◽  
Vol 49 (No. 4) ◽  
pp. 176-182 ◽  
Author(s):  
S. Samal ◽  
G.R. Rout ◽  
P.C. Lenka
Keyword(s):  
Rapd Markers ◽  
Genetic Relationships ◽  
Morphological Characters ◽  
The Other ◽  
Similarity Coefficients ◽  
Phenotypic Characters ◽  
Rapd Profile ◽  
Major Cluster ◽  
Similarity Indices ◽  
New Varieties

In the present paper genetic relationships of twenty varieties of cashew are described on the basis of morphological characters and RAPD (Randomly Amplified Polymorphic DNA) markers. Results obtained for the phenotypic characters based on similarity coefficient were divided into four clusters with 70% similarity. By means of similarity coefficients (SG), cluster I was found to consist of twelve varieties. Cluster II consisted of a single variety, NRCC-1, cluster III consisted of six varieties and cluster IV had only one variety, Vridhachalam-2. The analysis started by using RAPD markers that allowed us to distinguish 20 varieties. A total of 80 distinct DNA fragments ranging from 0.2 to 3.0 kb were amplified by using 11 selected random 10-mer primers. Genetic similarity analysis was conducted for the presence or absence of bands in the RAPD profile. Cluster analysis clearly showed that 20 varieties of cashew grouped into two major clusters based on similarity indices. The first major cluster comprised one minor cluster. The other major cluster was divided into two sub-minor clusters, one sub-minor cluster having three varieties and the other sub-minor cluster was represented by 15 varieties. Among the 20 varieties, Ullal-3 and Dhana (H-1608) showed the highest similarity indices (87%). It was noted that Vengurla-2 and Vengurla-3 were not grouped into a single cluster but Vengurla-4 has 82% similarity to Vengurla-3. The variety Vengurla-2 has very close similarity (85%) with variety Vridhachalam-3 (M-26/2). The analysis of genetic relationships in cashew using morphological traits and RAPD banding data can be useful for plant improvement, descriptions of new varieties and also for assessment of variety purity in plant certification programmes.  

scholarly journals Molecular characterization of Canna indica L. based on random amplified polymorphic DNA markers

2021 ◽  
Vol 28 (1) ◽  
pp. 75-81
Author(s):  
Sabiha Sultana Akhi ◽  
Bivas Kumar Sarkar ◽  
Nahid Sultana ◽  
Zakya Sultana Jui ◽  
RH Sarker ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Dna Markers ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
The Other ◽  
Canna Indica ◽  
Plant Taxon ◽  
The Relationship

Random amplified polymorphic DNA (RAPD) markers were employed for characterization, assessment of genetic variation and inferring relationships among six variants of Canna indica L. A total of 198 RAPD bands ranging from 200 bp to 3 kbp were generated by all the six variants. Among them, most of the bands were found to be polymorphic, four band were unique of which two bands (OPA022000 and OPA043000) were observed in the variant 2 (small red) and the other two (OPA013000 and OPA053000) were noticed in the variant 4 (orange), and the remaining bands were found to be monomorphic. The pair-wise genetic distance was determined among the six variants that ranged from 0.1446 to 0.6554. A dendogram was constructed based on the RAPD profiling to infer the relationship among the six variants of C. indica that resulted in two major clusters: the first one contained two variants, viz. variant 1 (local red) and variant 2 (small red), while the second cluster composed of the remaining four variants. The results as revealed from the RAPD analysis were found congruent with those of morphological and anatomical investigation of the species. Bangladesh J. Plant Taxon. 28(1): 75-81, 2021 (June)

scholarly journals RAPD Analysis of Hosta Species and Cultivars

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 811E-811
Author(s):  
Jing-Tian Ling ◽  
Nick Gawel ◽  
Roger J. Sauve
Keyword(s):  
Principal Components Analysis ◽  
Principal Components ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
The Other ◽  
Herbaceous Plant ◽  
Phylogenic Analysis ◽  
Components Analysis

The genus of Hosta (plantain lily) is a shade-loving herbaceous plant with attractive foliage. Confusion exists in the genus regarding nomenclature and taxonomy. In this study, the possibility of application of RAPD markers to characterize Hosta species and cultivars was investigated. DNA was extracted from 28 Hosta species and cultivars. Thirty-six of 37 primers generated RAPD markers. Phylogenic analysis and principal components analysis showed groupings among cultivars. Results indicated that H. plantaginea and H. ventricosa were the most distant from the other tested species and cultivars. These results suggest RAPDs may be useful in the identification and analysis of relationships among Hosta.

A study of cometary eruptions through OH radio-lines

1999 ◽  
Vol 173 ◽  
pp. 249-254
Author(s):  
A.M. Silva ◽  
R.D. Miró
Keyword(s):  
Short Duration ◽  
Growth Curves ◽  
The Other ◽  
Initial Mass ◽  
Radio Lines ◽  
Other Hand ◽  
Sudden Rise

AbstractWe have developed a model for theH2OandOHevolution in a comet outburst, assuming that together with the gas, a distribution of icy grains is ejected. With an initial mass of icy grains of 108kg released, theH2OandOHproductions are increased up to a factor two, and the growth curves change drastically in the first two days. The model is applied to eruptions detected in theOHradio monitorings and fits well with the slow variations in the flux. On the other hand, several events of short duration appear, consisting of a sudden rise ofOHflux, followed by a sudden decay on the second day. These apparent short bursts are frequently found as precursors of a more durable eruption. We suggest that both of them are part of a unique eruption, and that the sudden decay is due to collisions that de-excite theOHmaser, when it reaches the Cometopause region located at 1.35 × 105kmfrom the nucleus.

Closing the GAP—A 5Å Scanning Microscope

1969 ◽  
Vol 27 ◽  
pp. 6-7
Author(s):  
A. V. Crewe
Keyword(s):  
Normal Form ◽  
The Other ◽  
Resolving Power ◽  
Scanning Microscope ◽  
Conventional Microscope ◽  
Other Hand ◽  
Closing The Gap ◽  
Thermal Sources ◽  
Better Than ◽  
Transmission Microscope

We have become accustomed to differentiating between the scanning microscope and the conventional transmission microscope according to the resolving power which the two instruments offer. The conventional microscope is capable of a point resolution of a few angstroms and line resolutions of periodic objects of about 1Å. On the other hand, the scanning microscope, in its normal form, is not ordinarily capable of a point resolution better than 100Å. Upon examining reasons for the 100Å limitation, it becomes clear that this is based more on tradition than reason, and in particular, it is a condition imposed upon the microscope by adherence to thermal sources of electrons.

Sign in / Sign up

Export Citation Format

Share Document