scholarly journals Cultivation of Cypripedium calceolus L. ex vitro seedlings in outdoor conditions: Short communication

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 162-170
Author(s):  
J. Obdržálek
Keyword(s):  
Average Length ◽  
Additional Treatment ◽  
Ex Vitro ◽  
Private Laboratory ◽  
Cypripedium Calceolus ◽  
Pumice Sand ◽  
Bare Root ◽  
Lime Powder

The paper presents first positive results of the experiment with three-year outdoor cultivation of Cypripedium calceolus L. ex vitro seedlings in the Czech Republic. They were propagated in vitro from mature seeds of Carpathian provenance in a Prague private laboratory. In April 2006 after three months cool refrigerating at 4°C the seedlings were prepared for planting. The rhizomes with 4 to 12 roots and visible dormant buds were used. They were planted in two types of substrates: mixture AN on the basis of liadrain (burned clay pebbles) and mixture BN on the basis of granodiorite. Both mixtures were amended with perlite, pumice, sand, zeolite and dolomite lime powder. The mineral substrates proved to be stable and convenient for transfer and cultivation of ex vitro seedlings. Additional treatment with lignohumate in other two variants of the experiment did not improve the effect. The seedlings were grown outdoors on a shaded bed till the retracting leaves. They overwintered in a cold glasshouse with temperature close to zero from late November to March. The substrates did not visually influence the phase of sprouting, the phase of growth and retracting of the plants. At the end of the third growing season the yield of 4-year-old seedlings with two to four leaves ranged from 83% to 98% in four variants. In November 2008 seedlings were taken up from the mixes and were evaluated as bare root plants. The number of the living plants with visible new buds and the quality of root system were recorded and evaluated. The average length of roots in mixture A on the basis of liadrain and B on the basis of granodiorite was 14.5 cm and 12.1 cm, respectively. The rhizomes were planted into new mixtures immediately. These seedlings will be able to grow up to the blooming size during two or three seasons. Seven year-old potted seedlings of C. calceolus will be planted into gene resource area of the Silva Tarouca Research Institute for Landscape and Ornamental Gardening at Průhonice.

scholarly journals Effects of Hormonal and Basal Nutrient Medium on In vitro Regeneration of an Ornamental Plant - Muscari armeniacum Leichtlin. ex Baker

2013 ◽  
Vol 22 (2) ◽  
pp. 113-126 ◽  
Author(s):  
Mustafa Abul Kalam Azad ◽  
Muhammad Nurul Amin
Keyword(s):  
In Vitro Propagation ◽  
Average Length ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Leaf Sheath ◽  
Ornamental Plant ◽  
Garden Soil ◽  
Ex Vitro ◽  
Auxin Concentration

In vitro propagation system has been developed for an important ornamental and medicinal plant, Muscari armeniacum Leichtil. ex Bak. A range of a cytokinin and auxin concentration has been investigated for axillary bulblet proliferation, and direct and indirect adventitious bulblet regeneration from the explants whole bulb, one fourth part of bulb, bulb-scale of ex vitro (field grown mature bulb), and only leaf-sheath explants of in vitro grown bulblet. Axillary bulblet regeneration occurred on MS containing 2.0 - 8.0 ?M BAP or Kn. Direct adventitious bulblets were induced successfully on MS basal medium supplemented with various concentrations of BAP or Kn (1.0 - 4.0 ?M) in combi-nation of either NAA, IBA, or 2,4-D (0.5 - 4.0 ?M). The maximum frequency of adventitious bulblets regeneration occurred from both bulb-scale and leaf-sheath explants on MS with 4.0 ?M BAP and 2.0 ?M NAA, IBA, or 2,4-D. The highest frequency (95.5%) of indirect adventitious bulblets was obtained from in vitro grown leaf-sheathderived callus on MS containing 4.0 ?M BAP with 1.0 ?M 2,4-D whereas, highest number (80.2) and average length (55.5 cm) of bulblets were obtained on MS supplemented with 4.0 ?M BAP and 1.0 ?M NAA. In vitro grown bullets was rooted successfully on MS with 0.5 - 4.0 ?M of IBA, NAA, or IAA. The rooted bulblets were transferred to garden soil and successfully established under ex vitro environment. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14200 Plant Tissue Cult. & Biotech. 22(2): 113-126, 2012 (December)

scholarly journals Pengaruh Pupuk Daun “Organik” terhadap Komposisi Kimiawi dan Kecernaan Rumput Gajah (Pennisetum purpureum CV. Hawaii)

2020 ◽  
Vol 7 (3) ◽  
pp. 179
Author(s):  
Badat Muwakhid ◽  
Usman Ali
Keyword(s):  
Average Length ◽  
Organic Fertilizer ◽  
Neutral Detergent Fiber ◽  
Pennisetum Purpureum ◽  
Elephant Grass ◽  
Acid Detergent Fiber ◽  
Dry Matter Digestibility ◽  
Randomized Design

ABSTRAK Pupuk cair yang memanfaatkan bahan organik seperti urin yang difermentasi dan zat pengatur tumbuh dapat memberikan tambahan unsur hara yang diperlukan rumput untuk tumbuh. Upaya perbaikan kualitas rumput gajah dapat dilakukan dengan pemberian pupuk daun “organik”, dengan memperhatikan dosis pemberiannya. Tujuan penelitian ini adalah menentukan dosis pemberian pupuk daun “organik” yang tepat untuk mendapatkan kualitas hijauan yang maksimal. Materi penelitian ini meliputi pupuk daun “organik” dan bibit rumput gajah dalam keadaan pols, dengan rata-rata panjang perpols adalah 10 cm. Penelitian menggunakan metode percobaan dengan menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan masing-masing perlakuan menggunakan 5 kali ulangan. Perlakuan yang diberikan adalah P0 (kontrol), P1 (5% pupuk daun “organik”), P2 (10% pupuk daun “organik”) dan P3 (15% pupuk daun “organik”). Hasil penelitian ini menunjukkan bahwa perlakuan pemberian dosis pupuk daun “organik” yang berbeda memperikan pengaruh yang sangat nyata (P<0,01) terhadap kandungan Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), selulosa, hemiselulosa, lignin, kecernaan in vitro bahan kering dan kecernaan in vitro bahan organik rumput gajah. Berdasarkan penelitian ini pemberian dosis 15% pupuk daun “organik” menunjukkan hasil yang paling baik.Kata kunci: komposisi kimiawi. pupuk daun organik, rumput gajah, , kecernaanABSTRACTLiquid fertilizers using organic ingredients such as bio urine can provide additional nutrients to optimize the grow of grass. Efforts to improve the quality of elephant grass can be done by providing this organic fertilizer by calculating the dosage given. The purpose of this study was to determine the appropriate dosage of “organic” leaf fertilizers to obtain maximum forage quality. The research material used were “organic” leaf fertilizers and elephant grass seedlings in a state of pols, with an average length of each pols is 10 cm. The research method is experiment using a completely randomized design (CRD) which consisted of 4 treatments and 5 repetitions. The treatments given in this study were P0 (control), P1 (5% “organic” leaf fertilizers), P2 (10% “organic” leaf fertilizers) and P3 (15% “organic” leaf fertilizers). The results of this study indicate that the implementation of different dosages of “organic” leaf fertilizers had significant effect (P <0.01) on the content of Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), cellulose, hemicellulose, lignin, dry matter digestibility (in vitro) and organic matter digestibility (in vitro) of elephant grass. Based on this study, 15% dose of “organic” leaf fertilizers showed the best results.Keywords: chemical compounds, digestibility, elephant grass, organic leaf fertilizers

scholarly journals Studi Kualitas Regeneran Phalaenopsis Hasil Kultur In Vitro dari Eksplan Tangkai Infloresen, Tunas Pucuk, dan Empulur (The Quality Study of Phalaenopsis Regenerants from In Vitro Propagation of Inflorescence, Shoot Tip, and Pith Explants)

2019 ◽  
Vol 28 (1) ◽  
pp. 13
Author(s):  
Dewi Pramanik ◽  
Herni Shintiavira ◽  
Budi Winarto
Keyword(s):  
Clonal Propagation ◽  
Callus Formation ◽  
Stomatal Density ◽  
Average Length ◽  
Block Design ◽  
Shoot Tips ◽  
The Impact ◽  
In Vitro Clonal Propagation

<p>Anggrek <em>Phalaenopsis</em> memiliki nilai komersial yang tinggi, karena keindahannya dapat dinikmati sepanjang tahun. Hal tersebut berdampak pada kebutuhan benih tanaman yang semakin meningkat. Salah satu cara penyediaan benih secara massal adalah melalui perbanyakan klonal secara in vitro sehingga perlu dilakukan studi kualitas regeneran hasil perbanyakan klonal untuk menjamin ketersediaan benih dengan kualitas baik. Penelitian bertujuan menguji kualitas regeneran yang dihasilkan dari perbanyakan klonal secara in vitro beberapa varietas Phalaenopsis dengan menggunakan eksplan yang berbeda. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Kebun Percobaan Segunung, Balai Penelitian Tanaman Hias (Balithi) sejak bulan Januari 2014 hingga Mei 2015. Penelitian menggunakan dua faktor, yaitu varietas (Ayu Lestari, Ayu Pratiwi, dan Karindra) dan jenis eksplan (tangkai infloresen, tunas pucuk, dan empulur). Percobaan disusun menggunakan rancangan acak kelompok pola faktorial dan setiap perlakuan diulang tiga kali. Hasil penelitian menunjukan tidak terjadi interaksi yang nyata antara faktor jenis eksplan dan varietas yang diujikan pada semua tahap percobaan. Respon terbaik diperoleh pada eksplan empulur dengan 42,85% eksplan berhasil membentuk kalus pada minggu ke-8 dan hampir 100% kalus tersebut dapat beregenerasi menjadi tunas pada minggu ke-24 dengan tingkat multiplikasi tunas 1,87 kali. Pada minggu ke-32 terbentuk rata-rata 3,13 daun per planlet dengan 2,47 cm panjang daun, 1,36 cm lebar daun, 1,52 akar per planlet, dan panjang akar per planlet mencapai 1,26 cm. Kerapatan stomata memiliki korelasi negatif dengan tingkat abnormalitas planlet. Planlet dengan kerapatan stomata tertinggi dan abnormalitas yang rendah diperoleh pada var. Karindra dan planlet yang berasal dari eksplan empulur dan tunas pucuk. Setelah 8 minggu tahap aklimatisasi, tingkat keberhasilan hidup tertinggi (92%) diperoleh pada tunas yang berasal dari eksplan empulur. Penelitian membuktikan bahwa perbedaan varietas tidak memiliki pengaruh nyata pada tingkat abnormalitas regeneran dan dari eksplan empulur diperoleh jumlah regeneran tertinggi dengan kualitas baik (tingkat abnormalitas rendah).</p><p><strong>Keywords</strong></p><p>Kultur jaringan; Kualitas regeneran; <em>Phalaenopsis</em>; Jenis eksplant</p><p><strong>Abstract</strong></p><p><em>Phalaenopsis</em> orchids have a high commercial value, because of its beauty and it can be enjoyed throughout the year. This condition gives the impact on the increasing demand of the seeds. One of the ways of providing mass seeds is through in vitro clonal propagation. However, it is necessary to study the quality of regenerants of clonal propagation products to ensure the availability of qualified seeds. The aimed of this study was to test the quality of regenerants obtained from in vitro clonal propagation of Phalaenopsis using inflorescence stalk, shoot tips, and pith explants. This research was conducted at Tissue Culture Laboratory, Segunung Experimental Station, Indonesian Ornamental Crops Research Institute (IOCRI) from January 2014 to May 2015. The study used two treatments, varieties (Ayu Lestari, Ayu Pratiwi, and Karindra) and type of explant (inflorescence stalk, shoot tips, and pith). Experiments were prepared using a randomized complete block design with two factors and each treatment was replicated three times. The results showed there were no significant interaction between types of explants and varieties tested in all experiment stages. The best response was obtained using pith explants with 42.85% callus formation in the week eighth and nearly 100% callus can regenerate into shoots at week 24th with the rate of shoot multiplication up to 1.87 times. At week 32th the cultures formed planlets with an average number of leaves of 3.13 and an average size of 2.47 cm x 1.36 cm (length x width) and an average number of roots of 1.52 with average length reached 1.26 cm. Stomatal density has negative correlation with plantlet abnormality rate. Plantlets with the highest stomatal density and low abnormality were obtained in var. Karindra and plantlet derived from explant pith and shoot buds. After 8 weeks of acclimatization stage, the highest survival rate (92%) was obtained on the shoot originating from pith explant. This study proved that varietal differences did not have a significant effect on regenerant abnormalities, and the highest number of regenerant with good quality (low abnormality rate) was obtained from pith explant.</p>

Patrones de crecimiento de Cinchona officinalis in vitro y ex vitro; respuestas de plántulas micropropagadas y de semillas.

2017 ◽  
Vol 35 (1-2) ◽  
pp. 73-82
Author(s):  
Carlos Iván Espinosa ◽  
Gabriel Ríos
Keyword(s):  
Ex Vitro

El uso de herramientas biotecnológicas como la micropropagación se constituye en una alternativa de reproducción de especies amenazadas y con tamaños poblacionales reducidos. Sin embargo, uno de los problemas críticos en el uso de la micropropagación como herramienta de reproducción es la calidad de las plántulas resultantes en cuanto a su crecimiento y vigor. En el presente trabajo se evalua los efectos de la micropropagación sobre los patrones de crecimiento y sobrevivencia de plán­tulas in vitro de Cinchona officinalis L., una especie que ha sido fuertemente impactada por procesos de tala dentro de bosques naturales durante la época de la colonia. Se realizó un monitoreo de un total de 120 plántulas in vitro y 1988 plántulas ex vitro por 8 meses a partir del último repique. Adi­cionalmente, en cada plántula se contabilizó la cantidad de brotes axilares. Los resultados obtenidos mostraron un efecto remanente de los procesos de micropropagación, los cuales inicialmente inciden en la cantidad de brotes de las plántulas y en el crecimiento; sin embargo, este efecto no influye de forma negativa en la sobrevivencia de las plántulas durante la fase ex vitro

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