scholarly journals Expression of NR3C1, INSR and SLC2A4 genes in skeletal muscles and CBG in liver depends on age and breed of pigs

2019 ◽  
Vol 64 (No. 8) ◽  
pp. 343-351
Author(s):  
Dawid Szczepankiewicz ◽  
Marek Skrzypski ◽  
Ewa Pruszyńska-Oszmałek ◽  
Sławomir Sadkowski ◽  
Maciej Sassek ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Skeletal Muscles ◽  
Glucose Transporter ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Longissimus Dorsi Muscle ◽  
Muscle Tissues ◽  
Genes Encoding ◽  
Intramuscular Fat Content

The genes encoding glucose transporter 4 (SLC2A4, GLUT4), insulin receptor (INSR) and glucocorticoid receptor (NR3C1) are considered regulators of energy metabolism that may influence fat content in skeletal muscle at different ages and breeds of pigs. In the study we performed analysis of expression of NR3C1, INSR and SLC2A4 genes in two skeletal muscle tissues: semimembranosus and longissimus dorsi muscle in gilts from three breeds of pigs that differed in intramuscular fat content: Duroc (DU), Pulawska (PUL) and Polish Large White (PLW) at 60, 90, 120, 150, 180 and 210 days after weaning. We also analyzed the expression of cortisol binding globulin (CBG) in the liver. Expression was analyzed with real time PCR (qPCR) using a relative quantification method. In blood, the concentration of cortisol, insulin and leptin were evaluated with radioimmunoassay. The concentration of metabolites (triglycerides and glucose) related to the changes of lipids content was also measured. We observed a significant relationship between the breed and the age of pigs and the expression of analyzed genes in muscle tissues (P < 0.01 for all analyzed genes) as well as with two hormones (for insulin P = 0.001; for cortisol P < 0.0001). Leptin level and metabolites in serum were not significant in the model (P > 0.05). The expression of analyzed genes (SLC2A4, INSR and NR3C1 in muscles and CBG in liver) strongly correlates with intramuscular fat content in pigs and is associated with age and breed.

scholarly journals Variability in the leptin, leptin receptor and heart fatty acid binding protein genes in relationship with meat quality traits in pigs

2005 ◽  
Vol 53 (4) ◽  
pp. 87-94
Author(s):  
Renata Mikolášová ◽  
Tomáš Urban
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Binding Protein ◽  
Leptin Receptor ◽  
Binding Protein ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Intramuscular Fat Content

The leptin (LEP-HinfI), leptin receptor (LEPR-HpaII) and heart fatty acid binding protein (H-FABP-HinfI) genes and their genotypes combination (LEP-HinfI *LEPR-HpaII) were tested for associations with the pH1, pH24, myoglobin content (mg/100 g), intramuscular fat content (%) and remission (%). The genotypes were determined in Large White, Landrace and Duroc breeds (n = 106, 56 and 4, respectively). The allele frequencies were: LEP-HinfI: C = 0.133 T = 0.867; LEPR-HpaII: A = 0.331 B = 0.669; H-FABP-HinfI: H = 0.745 h = 0.255. The populations of breeds were in the genetic equilibrium according to the χ2 test in the tested loci. The combinations of LEP-HinfI and LEPR-HpaII were significantly associated with the pH24 and remission. The H-FABP-HinfI locus was significantly associated with intramuscular fat content.

scholarly journals Data from proteomic analysis of bovine Longissimus dorsi muscle associated with intramuscular fat content

Data in Brief ◽  
2018 ◽  
Vol 19 ◽  
pp. 1314-1317 ◽  
Author(s):  
Mirele D. Poleti ◽  
Luciana C.A. Regitano ◽  
Gustavo H.M.F. Souza ◽  
Aline S.M. Cesar ◽  
Rosineide C. Simas ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Intramuscular Fat Content

scholarly journals Muscle transcriptome analysis identifies genes involved in ciliogenesis and the molecular cascade associated with intramuscular fat content in Large White heavy pigs

PLoS ONE ◽  
2020 ◽  
Vol 15 (5) ◽  
pp. e0233372 ◽  
Author(s):  
Martina Zappaterra ◽  
Silvia Gioiosa ◽  
Giovanni Chillemi ◽  
Paolo Zambonelli ◽  
Roberta Davoli
Keyword(s):  
Transcriptome Analysis ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Intramuscular Fat Content

Ectopic overexpression of porcine DGAT1 increases intramuscular fat content in mouse skeletal muscle

2012 ◽  
Vol 22 (1) ◽  
pp. 187-194 ◽  
Author(s):  
Ting Li ◽  
Dequan Xu ◽  
Bo Zuo ◽  
Minggang Lei ◽  
Yuanzhu Xiong ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Mouse Skeletal Muscle ◽  
Ectopic Overexpression ◽  
Intramuscular Fat Content

scholarly journals Haplotype analysis within quantitative trait locus affecting intramuscular fat content on porcine chromosome

2011 ◽  
Vol 56 (No. 12) ◽  
pp. 521-528
Author(s):  
S. Sato ◽  
C. Ohnishi ◽  
Y. Uemoto ◽  
E. Kobayashi
Keyword(s):  
Quantitative Trait ◽  
Haplotype Analysis ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Porcine Chromosome ◽  
Large White ◽  
Positional Candidate ◽  
Synonymous Substitutions ◽  
Trait Locus ◽  
Intramuscular Fat Content

Previous results of fine mapping for quantitative trait loci affecting intramuscular fat content identified a 3.0-Mb chromosome interval on porcine chromosome 7, which contains at least 9 genes, based on the pig genome assembly. Therefore, we proposed these nine genes (LOC100154481, LOC100155711, LOC100155276, SPATA7, PTPN21, ZCH14, EML5, TTC8, and FOXN3) as positional candidate genes. The coding exons of the nine genes were characterized, and 45 polymorphisms were detected in F<sub>2</sub> Duroc &times; Meishan population. Within the nine genes, 10 non-synonymous substitutions and 1 insertion were genotyped among three European breeds (Landrace, Large White, and Duroc) and 1 Chinese breed (Meishan). Genotyping data was used to perform the haplotype analysis. Polymorphisms were found in all the studied genes, except ZCH14. We surveyed the frequency of 33 haplotypes that formed non-synonymous substitutions in four breeds. One of them was distributed widely in the Landrace, Large White, and Meishan breeds, but not in Duroc. Each breed had different major haplotypes. &nbsp;

scholarly journals Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc × Large White cross

BMC Genetics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 55 ◽  
Author(s):  
Marie-Pierre Sanchez ◽  
Nathalie Iannuccelli ◽  
Benjamin Basso ◽  
Jean-Pierre Bidanel ◽  
Yvon Billon ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Large White ◽  
Intramuscular Fat Content

scholarly journals Comparative Microbial Profiles of Colonic Digesta between Ningxiang Pig and Large White Pig

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1862
Author(s):  
Linfeng Lei ◽  
Zhaobin Wang ◽  
Jianzhong Li ◽  
Huansheng Yang ◽  
Yulong Yin ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Fatty Acid Biosynthesis ◽  
16S Rrna Gene Sequencing ◽  
Short Chain Fatty Acids ◽  
Intramuscular Fat ◽  
Fat Content ◽  
Rrna Gene ◽  
Scaling Analysis ◽  
Large White ◽  
Intramuscular Fat Content

Sixteen 35-day-old piglets, including eight Large White (LW) piglets (a lean-type pig breed) and eight Ningxiang (NX) piglets (a fatty-type Chinese Indigenous pig breed), were fed the same diet for 105 days. NX pigs had higher intramuscular fat content than LW pigs (p < 0.05). According to 16S rRNA gene sequencing, the relative abundances of the genera Ruminococcaceae_NK4A214_group, Parabacteroides, Christensenellaaceae_R-7_group and Ruminiclostridium were higher, whereas the abundances of Prevotellaceae_NK3B31_group, Prevotella, Subdoligranulum and Faecalibacterium were lower, in the colon of NX pigs compared to that of LW pigs. Nonmetric multidimensional scaling analysis revealed that the microbiota of the two pig breeds clustered separately along the principal coordinate axis. Furthermore, functional prediction of the bacterial communities suggested higher fatty acid biosynthesis in NX pigs. NX pigs also exhibited lower concentrations of total short-chain fatty acids, propionate and butyrate in the colon (p < 0.05). These findings suggest that NX pigs exhibited higher intramuscular fat content and backfat thickness than LW pigs. The bacterial communities in the colon of NX pigs were also more diverse than those in the colon of LW pigs, which might be used as a potential metabolomics mechanism to research different breeds of pigs.

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