Differential expression profiling of estrogen receptor in the ovaries of two egg duck (Anas platyrhynchos) breeds

In the present study, quantitative real-time PCR was employed to investigate expression profiling and expression difference of ESR1 and ESR2 in ovaries of Shaoxing duck and Jingjiang duck during three laying stages. Results showed the expression levels of ESR1 and ESR2 in ovaries were increased from the age of the first egg to the age of 500 days in both duck breeds. The expression of ESR1 in Shaoxing duck was lower than that in Jingjiang duck for the age of the first egg and of 180 days, and for the age of 500 days it was higher in Shaoxing duck than in Jingjiang duck. The ESR2 showed converse expression profiling in the two duck breeds. The results suggest that ESR1 and ESR2 mediate the process of egg laying in ducks, and that ESR2 may play a more important role for the ovary during egg-laying stages and may be closely related to the laying performance of the ducks.    

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Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR

Cancer Cell International ◽

10.1186/1475-2867-10-2 ◽

2010 ◽

Vol 10 (1) ◽

pp. 2 ◽

Cited By ~ 31

Author(s):

Takashi Watanabe ◽

Tomohiro Miura ◽

Yusuke Degawa ◽

Yuna Fujita ◽

Masaaki Inoue ◽

...

Keyword(s):

Gene Expression ◽

Lung Cancer ◽

Gene Expression Profiling ◽

Real Time ◽

Cell Lines ◽

Cancer Cell ◽

Real Time Pcr ◽

Expression Profiling ◽

Quantitative Real Time Pcr ◽

Lung Cancer Cell

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Comparing MicroRNA Profilings of Purified HER-2-Negative and HER-2-Positive Cells Validates miR-362-5p/Sema3A as Characteristic Molecular Change in Triple-Negative Breast Cancers

Disease Markers ◽

10.1155/2019/6057280 ◽

2019 ◽

Vol 2019 ◽

pp. 1-12 ◽

Cited By ~ 1

Author(s):

Xiaoqing Zhang ◽

Qi He ◽

Leiqin Sun ◽

Yanfei Zhang ◽

Shengying Qin ◽

...

Keyword(s):

Breast Cancer ◽

Protein Expression ◽

Real Time ◽

Differential Expression ◽

Real Time Pcr ◽

Therapeutic Target ◽

Triple Negative ◽

Quantitative Real Time Pcr ◽

Her 2 ◽

Tnbc Subtype

Background. HER-2 is a key molecule serving as the therapeutic target, prognostic biomarker, and classification marker in breast cancer. Accurate microRNA profilings had not been conducted in purified tumor cells of HER-2-negative and HER-2-positive tissue specimens obtained from breast cancer patients. Methods. (i) Differential expression microRNA discovery using laser capture microdissection- (LCM-) assisted specimen preparation and microRNA array chips on HER-2 overexpressing and triple-negative breast carcinoma (TNBC) subtype tissues, (ii) differential expression microRNA validation by quantitative real-time PCR, and (iii) independent validation on tissue microarray. Results. Five microRNAs (miR-20a-5p, miR-221-3p, miR-362-5p, miR-502-3p, and miR-222-3p) were screened and validated as upregulated microRNAs in TNBC cells comparing to HER-2 overexpressing cells using a microRNA array (5 cases in each group) and quantitative real-time PCR (20 cases in each group). The expression difference of miR-362-5p had the most significant statistical significance (p=0.0016) among the five microRNAs. The expression of miR-362-5p and its target gene Sema3A was further analyzed using in situ hybridization (ISH) and immunohistochemistry on standard tissue sections (n=150). 70.8% of HER-2-negative cells showed moderate expression of miR-362-5p whereas 20.4% HER-2-negative cells correlated with strong expression of miR-362-5p (p<0.0001). The proportion of patients with moderate/strong miR-362-5p expression in luminal, HER-2 overexpressing, and TNBC subtypes were 53.2%, 22.2%, and 74.3%, respectively (p=0.0002). High miR-362-5p expressers had shorter overall survival in the univariate analysis (p=0.046). There was a significant negative correlation between miR-362-5p and Sema3A expression (p<0.0001). The patients with negative/weak Sema3A protein expression had poorer prognosis than those with moderate (HR: 3.723, p=0.021) or strong (HR: 3.966, p=0.013) Sema3A protein expression in the multivariate analysis. Conclusions. miR-362-5p/Sema3A might provide a promising therapeutic pathway and represents a candidate therapeutic target of the TNBC subtype.

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Discrimination of genotoxic and non-genotoxic hepatocarcinogens by statistical analysis based on gene expression profiling in the mouse liver as determined by quantitative real-time PCR

Mutation Research/Genetic Toxicology and Environmental Mutagenesis ◽

10.1016/j.mrgentox.2012.04.011 ◽

2012 ◽

Vol 747 (2) ◽

pp. 164-175 ◽

Cited By ~ 25

Author(s):

Takashi Watanabe ◽

Takayoshi Suzuki ◽

Masakatsu Natsume ◽

Madoka Nakajima ◽

Kazunori Narumi ◽

...

Keyword(s):

Gene Expression ◽

Statistical Analysis ◽

Gene Expression Profiling ◽

Real Time ◽

Real Time Pcr ◽

Expression Profiling ◽

Mouse Liver ◽

Quantitative Real Time Pcr

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Expression Profiling of MicroRNAs by Quantitative Real-Time PCR

PCR Technology ◽

10.1201/b14930-29 ◽

2013 ◽

pp. 307-322 ◽

Cited By ~ 2

Author(s):

Mirco Castoldi ◽

Paul Collier ◽

Tania Nolan ◽

Vladimir Benes

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Expression Profiling ◽

Quantitative Real Time Pcr

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Circular RNA circDUS2 Is a Potential Biomarker for Intracranial Aneurysm

Frontiers in Aging Neuroscience ◽

10.3389/fnagi.2021.632448 ◽

2021 ◽

Vol 13 ◽

Author(s):

Xin Chen ◽

Shuzhe Yang ◽

Junhua Yang ◽

Qingyuan Liu ◽

Maogui Li ◽

...

Keyword(s):

Smooth Muscle ◽

Intracranial Aneurysm ◽

Vascular Smooth Muscle ◽

Real Time ◽

Differential Expression ◽

Real Time Pcr ◽

Superficial Temporal Artery ◽

Quantitative Real Time Pcr ◽

Temporal Arteries ◽

Potential Biomarker

Background: CircRNAs have been found to play a crucial role in the pathological process of various kinds of diseases. However, the role of circRNAs in the formation and rupture of intracranial aneurysm is still unknown.Methods: Differentially expressed circRNAs profiles between superficial temporal arteries (n = 5) and intracranial aneurysms (n = 5) were analyzed using the Arraystar human circRNAs microarray. Quantitative real-time PCR was utilized to validate the differential expression of circDUS2. Fluorescence in situ hybridization (FISH) was meant for the location of circDUS2 in human brain vascular smooth muscle cell (HBVSMC). Structural analysis was used to speculate on the function of circDUS2.Results: Five hundred forty-three upregulated and 397 downregulated significantly in intracranial aneurysm as compared to superficial temporal arteries. Quantitative real-time PCR verified the elevated expression of the upregulated circDUS2. The FISH test revealed that circDUS2 is located in the cytoplasm of brain vascular smooth muscle cells.Conclusion: This study showed differential expression data of circRNAs between superficial temporal artery and intracranial aneurysm and revealed that circDUS2 is a potential molecular marker for intracranial aneurysm.

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