scholarly journals Determination of Bacterial Cell Number to Differentiate Campylobacter jejuni from C. coli by the Simple Hippurate Hydrolysis Test

2012 ◽  
Vol 2 (9) ◽  
Author(s):  
Miyuki Fujioka ◽  
Yoshimitsu Otomo
Keyword(s):  
Campylobacter Jejuni ◽  
Bacterial Cell ◽  
Cell Number ◽  
Bacterial Cell Number

Antibacterial activity of the seminal plasma of Paracentrotus lividus

1994 ◽  
Vol 72 (7) ◽  
pp. 1211-1216 ◽  
Author(s):  
Loredana Stabili ◽  
Calogero Canicattì
Keyword(s):  
Antibacterial Activity ◽  
Seminal Plasma ◽  
Bacterial Growth ◽  
Bacterial Cell ◽  
Inhibitory Action ◽  
Paracentrotus Lividus ◽  
Cell Number ◽  
Morphological Examination ◽  
Bacterial Cell Number ◽  
Antibacterial Factor

Seminal plasma from Paracentrotus lividus exerted an inhibitory action on the growth of bacterial colonies. The antibacterial reaction took 30 min to reach full expression and depended on both the dose of seminal plasma and the bacterial cell number. Heating at 56 °C for 60 min did not lower the antibacterial power of the seminal plasma. Morphological examination of bacteria treated with seminal plasma revealed a conspicuous alteration of their surface and suggested a lytic mode of action for the antibacterial factor(s). Lysozyme could be involved in this process. In fact, inhibition of bacterial growth strongly decreased when this hydrolase was inactivated by heating at a basic pH.

scholarly journals Consideration of Influential Factors on Bioleaching of Gold Ore Using Iodide-Oxidizing Bacteria

Minerals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 274 ◽  
Author(s):  
San Yee Khaing ◽  
Yuichi Sugai ◽  
Kyuro Sasaki ◽  
Myo Min Tun
Keyword(s):  
Potassium Iodide ◽  
Bacterial Cell ◽  
Cell Number ◽  
Culture Solution ◽  
Gold Dissolution ◽  
Marine Broth ◽  
Gold Leaching ◽  
Temperature Range ◽  
Gold Ore ◽  
Bacterial Cell Number

Iodide-oxidizing bacteria (IOB) oxidize iodide into iodine and triiodide which can be utilized for gold dissolution. IOB can be therefore useful for gold leaching. This study examined the impact of incubation conditions such as concentration of the nutrient and iodide, initial bacterial cell number, incubation temperature, and shaking condition on the performance of the gold dissolution through the experiments incubating IOB in the culture medium containing the marine broth, potassium iodide and gold ore. The minimum necessary concentration of marine broth and potassium iodide for the complete gold dissolution were determined to be 18.7 g/L and 10.9 g/L respectively. The initial bacterial cell number had no effect on gold dissolution when it was 1 × 104 cells/mL or higher. Gold leaching with IOB should be operated under a temperature range of 30–35 °C, which was the optimal temperature range for IOB. The bacterial growth rate under shaking conditions was three times faster than that under static conditions. Shaking incubation effectively shortened the contact time compared to the static incubation. According to the pH and redox potential of the culture solution, the stable gold complex in the culture solution of this study could be designated as gold (I) diiodide.

scholarly journals Efficacy Bacteriophage in Removal Pseudomonas aeruginosa from Infectious Surfaces

2021 ◽  
Author(s):  
Golnar Rahimzadeh ◽  
Mohammad Sadegh Rezai
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nosocomial Infection ◽  
Nosocomial Infections ◽  
Bacterial Cell ◽  
Cell Number ◽  
Biocontrol Agents ◽  
Hospital Environment ◽  
Bacterial Cells ◽  
Bacterial Cell Number

Nosocomial infections can be transmitted by contaminated hospital surfaces with resistant pathogens. conventional sanitations are not efficiently contributing to removing resistant pathogens. Bacteriophages suggest as decontaminating agents, safe, their selective ability to kill specific bacteria. This work aimed to assess the efficiency of a phage in removing Pseudomonas aeruginosa from different hard surfaces. The decontamination ability of phages w was tested in vitro against Pseudomonas aeruginosa strain. Cystoviridae Phages with titer (2 × 1012 PFU/mL) can efficiently reduce viable bacterial cells on contaminated surfaces. The treated surfaces with alcohol 70% and phage showed an evident drop of bacterial cell number from 1 h to 24 h. These results suggest that bacteriophages are biocontrol agents removing nosocomial infection pathogens transmitted by contaminated surfaces in the hospital environment.

scholarly journals Estimation of lactic acid bacterial cell number by DNA quantification

2018 ◽  
Vol 12 (2) ◽  
pp. 88-91 ◽  
Author(s):  
Masaki Ishii ◽  
Yasuhiko Matsumoto ◽  
Kazuhisa Sekimizu
Keyword(s):  
Lactic Acid ◽  
Bacterial Cell ◽  
Cell Number ◽  
Dna Quantification ◽  
Bacterial Cell Number

Development of a bioluminescent ATP assay to quantify mammalian and bacterial cell number from a mixed population

Biomaterials ◽  
2003 ◽  
Vol 24 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Sarah J. Dexter ◽  
Miguel Cámara ◽  
Martyn Davies ◽  
Kevin M. Shakesheff
Keyword(s):  
Bacterial Cell ◽  
Cell Number ◽  
Mixed Population ◽  
Atp Assay ◽  
Bacterial Cell Number

scholarly journals Development of biosensor for the determination of total viable bacterial cell count.

1991 ◽  
Vol 57 (2) ◽  
pp. 281-285 ◽  
Author(s):  
Masakazu Hoshi ◽  
Hideaki Nishi ◽  
Tetsuhito Hayashi ◽  
Masayo Okuzumi ◽  
Etsuo Watanabe
Keyword(s):  
Cell Count ◽  
Bacterial Cell ◽  
Viable Bacterial Cell

Advantage of menadione-catalyzed chemiluminescent assay for the determination of viable mammalian cell number

2012 ◽  
Vol 421 (2) ◽  
pp. 428-432 ◽  
Author(s):  
Shiro Yamashoji ◽  
Naoko Yoshikawa ◽  
Masayuki Kirihara ◽  
Toshihiro Tsuneyoshi
Keyword(s):  
Mammalian Cell ◽  
Cell Number

scholarly journals The cryo-EM structure of the bacterial flagellum cap complex suggests a molecular mechanism for filament elongation

2019 ◽  
Author(s):  
Natalie S. Al-Otaibi ◽  
Aidan J. Taylor ◽  
Daniel P. Farrell ◽  
Svetomir B. Tzokov ◽  
Frank DiMaio ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Campylobacter Jejuni ◽  
Bacterial Cell ◽  
Molecular Basis ◽  
Molecular Model ◽  
Molecular Motor ◽  
Terminal Region ◽  
Bacterial Flagellum ◽  
Filament Assembly ◽  
Oligomeric Assembly

AbstractThe bacterial flagellum is a remarkable molecular motor, present at the surface of many bacteria, whose primary function is to allow motility through the rotation of a long filament protruding from the bacterial cell. A cap complex, consisting of an oligomeric assembly of the protein FliD, is localized at the tip of the flagellum, and is essential for filament assembly, as well as adherence to surfaces in some bacteria. However, the structure of the intact cap complex, and the molecular basis for its interaction with the filament, remains elusive. Here we report the cryo-EM structure of the Campylobacter jejuni cap complex. This structure reveals that FliD is pentameric, with the N-terminal region of the protomer forming an unexpected extensive set of contacts across several subunits, that contribute to FliD oligomerization. We also demonstrate that the native C. jejuni flagellum filament is 11-stranded and propose a molecular model for the filament-cap interaction.

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