Staphilococcus Aureus Sampling v2

Author(s):  
Pol Roca Cugat
Keyword(s):  

This protocol is intended to study the affectation of Staphilococcus Aureus, including the MRSA variant. It outlines the basic protocol for a multi-subject study.

2012 ◽  
Vol 3 (2) ◽  
pp. 419-423
Author(s):  
JARUPULA RAJESHWAR ◽  
Dr G NARSIMHA

A freely moving nodes forming as group to communicate among themselves are called as Mobile AdHoc Networks (MANET). Many applications are choosing this MANET for effective commutation due to its flexible nature in forming a network. But due to its openness characteristics it is posing many security challenges. As it has highly dynamic network topology security for routing is playing a major role. We have very good routing protocols for route discovery as well as for transporting data packers but most of them lack the feature of security like AODV. In this paper we are studying the basic protocol AODV and identify how it can be made secure. We are studying a protocol S-AODV which is a security extension of AODV which is called Secure AODV (S-AODV) and we are studying enhanced version of S-AODV routing protocol a Adaptive Secure AODV (A-SAODV). Finally we have described about the parameter to be taken for performance evaluation of different secure routing protocols


2014 ◽  
Vol 21 (11) ◽  
pp. 1581-1588 ◽  
Author(s):  
Piotr Kardas ◽  
Mohammadreza Sadeghi ◽  
Fabian H. Weissbach ◽  
Tingting Chen ◽  
Lea Hedman ◽  
...  

ABSTRACTJC polyomavirus (JCPyV) can cause progressive multifocal leukoencephalopathy (PML), a debilitating, often fatal brain disease in immunocompromised patients. JCPyV-seropositive multiple sclerosis (MS) patients treated with natalizumab have a 2- to 10-fold increased risk of developing PML. Therefore, JCPyV serology has been recommended for PML risk stratification. However, different antibody tests may not be equivalent. To study intra- and interlaboratory variability, sera from 398 healthy blood donors were compared in 4 independent enzyme-linked immunoassay (ELISA) measurements generating >1,592 data points. Three data sets (Basel1, Basel2, and Basel3) used the same basic protocol but different JCPyV virus-like particle (VLP) preparations and introduced normalization to a reference serum. The data sets were also compared with an independent method using biotinylated VLPs (Helsinki1). VLP preadsorption reducing ≥35% activity was used to identify seropositive sera. The results indicated that Basel1, Basel2, Basel3, and Helsinki1 were similar regarding overall data distribution (P= 0.79) and seroprevalence (58.0, 54.5, 54.8, and 53.5%, respectively;P= 0.95). However, intra-assay intralaboratory comparison yielded 3.7% to 12% discordant results, most of which were close to the cutoff (0.080 < optical density [OD] < 0.250) according to Bland-Altman analysis. Introduction of normalization improved overall performance and reduced discordance. The interlaboratory interassay comparison between Basel3 and Helsinki1 revealed only 15 discordant results, 14 (93%) of which were close to the cutoff. Preadsorption identified specificities of 99.44% and 97.78% and sensitivities of 99.54% and 95.87% for Basel3 and Helsinki1, respectively. Thus, normalization to a preferably WHO-approved reference serum, duplicate testing, and preadsorption for samples around the cutoff may be necessary for reliable JCPyV serology and PML risk stratification.


PEDIATRICS ◽  
1989 ◽  
Vol 83 (2) ◽  
pp. A42-A42
Author(s):  
Student

[There are] two policy choices: we can abandon attempts to justify use of anencephalic infants as organ donors because there is currently no clinically accepted means to declare brain death in these infants; or we can carry out the research necessary to establish a clinically valid procedure for doing so. A Canadian group has decided to take the second route and experiment on methods to use as organ donors anencephalic newborns who can be validly declared brain-dead on classic criteria. The group has developed a basic protocol that calls for the parents to agree, prior to birth, that: (1) the infant will be resuscitated; (2) periodic testing will be done to determine brain death (removal from the ventilator at six-to-twelve-hour intervals for a ten-minute period to determine ability to breathe spontaneously; (3) organ donation is acceptable; and (4) a definite time limit (to be determined by the parents but not more than fourteen days) after which the infant will be removed from the ventilator and permitted to die. Low-dose morphine is administered to prevent potential suffering on the part of the infant, although whether anencephalic newborns can suffer is unknown.


2013 ◽  
Vol 28 ◽  
pp. 1
Author(s):  
M.A. Gonzalez-Torres ◽  
S. Bustamante ◽  
E. Vivanco ◽  
K. Martinez ◽  
S. Alvarez de Eulate ◽  
...  

2022 ◽  
Author(s):  
Pol Roca Cugat
Keyword(s):  

This protocol is intended to study the affectation of Staphilococcus Aureus, including the MRSA variant. It outlines the basic protocol for a multi-subject study.


2022 ◽  
Author(s):  
Jasmine Amirzadegan ◽  
tunc.kayikcioglu not provided ◽  
hugh.rand not provided ◽  
Ruth E Timme ◽  
Maria Balkey

PURPOSE: Step-by-step instructions for checking sequence quality for SARS-CoV-2 wastewater samples using SSQuAWK: SARS - CoV - 2 Sequence Quality Assurance Workflow and Kontraption. The SSQuAWK workflow, implemented in a custom Galaxy instance, will produce quality assessments for raw reads (Illumina MiSeq paired-end fastq files). SCOPE: This protocol covers the following tasks: 1. Set up an account in GalaxyTrakr 2. Create a new history 3. Upload data 4. Execute the SSQuAWK workflow 5. Interpret the results Version history: V1: Basic protocol steps with screenshots V2: Addition of a detailed 12 minute video tutorial


Author(s):  
Che-Hung Lin ◽  
Fang-Yan Dong ◽  
Kaoru Hirota

Abstract A protocol, called common driving notification protocol (CDNP), is proposed based on the classified driving behavior for intelligent autonomous vehicles, and it defines a standard with common messages and format for vehicles. The common standard format and definitions of CDNP packet make the autonomous vehicles have a common language to exchange more detail driving decision information of various driving situations, decrease the identification time for one vehicle to identify the driving decisions of other vehicles before or after those driving decisions are performed. The simulation tools, including NS- 3 and SUMO, are used to simulate the wireless data packet transmission and the vehicle mobility; the experiment results present that the proposed protocol, CDNP, can increase the reaction preparing time with maximum value 250 seconds, decrease the identification time and the average travel time. Prospectively, it is decided to implement the CDNP as a protocol stack in the Linux kernel to provide the basic protocol capability for real world transmission testing.


2007 ◽  
Vol 06 (02) ◽  
pp. 301-313 ◽  
Author(s):  
SEOKYUN JEONG ◽  
SUN HUR ◽  
JUNG-YONG KIM

Distributed coordination function (DCF) is a primary random access mechanism of IEEE 802.11, which is the basic protocol of wireless LAN based on the CSMA/CA protocol. It enables fast installation with minimal management and maintenance costs and is a very robust protocol for the best effort service in wireless medium. The current DCF, however, is known to be unsuitable for real-time applications such as voice message transmission. In this paper, we focus on the performance issues of IEEE 802.11 which accommodates the prioritized messages. Existing results use the initial window size and backoff window-increasing factor as tools to handle the priority of the messages. Instead, we introduce a novel scheme which chooses the backoff timer with arbitrary probabilities. By this, one can greatly reduce the backoff delay of the lower priority messages without degrading the performance of higher priority. Additionally, we provide a step-by-step procedure which determines the system parameters such as the initial window size, backoff window-increasing factor, and the backoff timer choosing probabilities, guaranteeing the quality of service (QoS).


2013 ◽  
Vol 28 ◽  
pp. 1
Author(s):  
M.A. Gonzalez-Torres ◽  
S. Bustamante ◽  
E. Vivanco ◽  
B. Trojaola ◽  
A. Fernandez-Rivas

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