Microfluidics 4: PDMS Chip Soft Lithography v2

2021 ◽  
Author(s):  
Serhat Sevli ◽  
not provided C. Yunus Sahan
Keyword(s):  
Soft Lithography ◽  
Cost Effective ◽  
Cnc Machining ◽  
3D Printed ◽  
Low Volume ◽  
Pdms Chip ◽  
Application Specific ◽  
Lithography Technique

Microfluidics materials are of various types and application-specific. PDMS is one of the most preferred and cost-effective solutions for research and low-volume manufacturing. After having the mold, PDMS replicas are generated by a technique called soft-lithography. This protocol describes the preparation of PDMS microchannels using SU8 molds, 3D Printed resin molds, and/or metal molds by the soft lithography technique, SLA printing, or CNC machining.

Microfluidics 4: PDMS Chip Soft Lithography v2

2021 ◽  
Author(s):  
Serhat Sevli ◽  
not provided C. Yunus Sahan
Keyword(s):  
Soft Lithography ◽  
Cost Effective ◽  
Cnc Machining ◽  
3D Printed ◽  
Low Volume ◽  
Pdms Chip ◽  
Application Specific ◽  
Lithography Technique

Microfluidics materials are of various types and application-specific. PDMS is one of the most preferred and cost-effective solutions for research and low-volume manufacturing. After having the mold, PDMS replicas are generated by a technique called soft-lithography. This protocol describes the preparation of PDMS microchannels using SU8 molds, 3D Printed resin molds, and/or metal molds by the soft lithography technique, SLA printing, or CNC machining.

scholarly journals 3D printed microfluidic lab-on-a-chip device for fiber-based dual beam optical manipulation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Haoran Wang ◽  
Anton Enders ◽  
John-Alexander Preuss ◽  
Janina Bahnemann ◽  
Alexander Heisterkamp ◽  
...  
Keyword(s):  
Optical Fibers ◽  
Single Cells ◽  
Lab On A Chip ◽  
Cost Effective ◽  
Optical Manipulation ◽  
Hydrodynamic Focusing ◽  
Trapping Region ◽  
Dual Beam ◽  
3D Printed ◽  
On Chip

Abstract3D printing of microfluidic lab-on-a-chip devices enables rapid prototyping of robust and complex structures. In this work, we designed and fabricated a 3D printed lab-on-a-chip device for fiber-based dual beam optical manipulation. The final 3D printed chip offers three key features, such as (1) an optimized fiber channel design for precise alignment of optical fibers, (2) an optically clear window to visualize the trapping region, and (3) a sample channel which facilitates hydrodynamic focusing of samples. A square zig–zag structure incorporated in the sample channel increases the number of particles at the trapping site and focuses the cells and particles during experiments when operating the chip at low Reynolds number. To evaluate the performance of the device for optical manipulation, we implemented on-chip, fiber-based optical trapping of different-sized microscopic particles and performed trap stiffness measurements. In addition, optical stretching of MCF-7 cells was successfully accomplished for the purpose of studying the effects of a cytochalasin metabolite, pyrichalasin H, on cell elasticity. We observed distinct changes in the deformability of single cells treated with pyrichalasin H compared to untreated cells. These results demonstrate that 3D printed microfluidic lab-on-a-chip devices offer a cost-effective and customizable platform for applications in optical manipulation.

scholarly journals 3D Printed Imaging Platform for Portable Cell Counting

The Analyst ◽  
2021 ◽  
Author(s):  
Diwakar M. Awate ◽  
Cicero C. Pola ◽  
Erica Shumaker ◽  
Carmen L Gomes ◽  
Jaime Javier Juarez
Keyword(s):  
3D Printing ◽  
Point Of Care ◽  
Cost Effective ◽  
Cell Counting ◽  
Biomedical Sciences ◽  
Widespread Application ◽  
Resource Limited ◽  
Cost Effective Approach ◽  
3D Printed

Despite having widespread application in the biomedical sciences, flow cytometers have several limitations that prevent their application to point-of-care (POC) diagnostics in resource-limited environments. 3D printing provides a cost-effective approach...

scholarly journals Effects of Rice Straw Powder (RSP) Size and Pretreatment on Properties of FDM 3D-Printed RSP/Poly(Lactic Acid) Biocomposites

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3234
Author(s):  
Wangwang Yu ◽  
Lili Dong ◽  
Wen Lei ◽  
Yuhan Zhou ◽  
Yongzhe Pu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Rice Straw ◽  
Fused Deposition Modeling ◽  
Flexural Modulus ◽  
Tensile Modulus ◽  
Cost Effective ◽  
Poly Lactic Acid ◽  
Fused Deposition ◽  
Interfacial Compatibility ◽  
3D Printed

To develop a new kind of environment-friendly composite filament for fused deposition modeling (FDM) 3D printing, rice straw powder (RSP)/poly(lactic acid) (PLA) biocomposites were FDM-3D-printed, and the effects of the particle size and pretreatment of RSP on the properties of RSP/PLA biocomposites were investigated. The results indicated that the 120-mesh RSP/PLA biocomposites (named 120#RSP/PLA) showed better performance than RSP/PLA biocomposites prepared with other RSP sizes. Infrared results showed that pretreatment of RSP by different methods was successful, and scanning electron microscopy indicated that composites prepared after pretreatment exhibited good interfacial compatibility due to a preferable binding force between fiber and matrix. When RSP was synergistically pretreated by alkaline and ultrasound, the composite exhibited a high tensile strength, tensile modulus, flexural strength, and flexural modulus of 58.59, 568.68, 90.32, and 3218.12 MPa, respectively, reflecting an increase of 31.19%, 16.48%, 18.75%, and 25.27%, respectively, compared with unmodified 120#RSP/PLA. Pretreatment of RSP also improved the thermal stability and hydrophobic properties, while reducing the water absorption of 120#RSP/PLA. This work is believed to provide highlights of the development of cost-effective biocomposite filaments and improvement of the properties of FDM parts.

Fabrication of Tunable Silk Materials Through Microfluidic Mixers

2016 ◽  
Author(s):  
Joseph R. Nalbach ◽  
Dave Jao ◽  
Douglas G. Petro ◽  
Kyle M. Raudenbush ◽  
Shibbir Ahmad ◽  
...  
Keyword(s):  
Soft Lithography ◽  
Mixing Processes ◽  
Microfluidic Mixing ◽  
Microfluidic Mixer ◽  
Continuous Mixing ◽  
Mixing Performance ◽  
3D Printed ◽  
Distribution Uniformity ◽  
Mixing Patterns ◽  
Microfluidic Mixers

A common method to precisely control the material properties is to evenly distribute functional nanomaterials within the substrate. For example, it is possible to mix a silk solution and nanomaterials together to form one tuned silk sample. However, the nanomaterials are likely to aggregate in the traditional manual mixing processes. Here we report a pilot study of utilizing specific microfluidic mixing designs to achieve a uniform nanomaterial distribution with minimal aggregation. Mixing patterns are created based on classic designs and then validated by experimental results. The devices are fabricated on polydimethylsiloxane (PDMS) using 3D printed molds and soft lithography for rapid replication. The initial mixing performance is validated through the mixing of two solutions with colored dyes. The microfluidic mixer designs are further analyzed by creating silk-based film samples. The cured film is inspected with scanning electron microscopy (SEM) to reveal the distribution uniformity of the dye particles within the silk material matrix. Our preliminary results show that the microfluidic mixing produces uniform distribution of dye particles. Because the microfluidic device can be used as a continuous mixing tool, we believe it will provide a powerful platform for better preparation of silk materials. By using different types of nanomaterials such as graphite (demonstrated in this study), graphene, carbon nanotubes, and magnetic nanoparticles, the resulting silk samples can be fine-tuned with desired electrical, mechanical, and magnetic properties.

Otta Seals and Gravseals as Low-Cost Surfacing Alternatives for Low-Volume Roads: Experiences in South Africa

2003 ◽  
Vol 1819 (1) ◽  
pp. 338-342 ◽  
Author(s):  
Simon Oloo ◽  
Rob Lindsay ◽  
Sam Mothilal
Keyword(s):  
South Africa ◽  
Low Cost ◽  
Cost Savings ◽  
Cost Effective ◽  
Field Trials ◽  
Low Volume Roads ◽  
Kwazulu Natal ◽  
Traffic Volumes ◽  
Low Volume ◽  
To Come

The geology of the northeastern part of the province of KwaZulu–Natal, South Africa, is predominantly alluvial with vast deposits of sands. Suitable gravel sources are hard to come by, which results in high graveling and regraveling costs brought about by long haul distances and accelerated gravel loss. Most gravel roads carry fewer than 500 vehicles per day of which less than 10% are heavy vehicles. The high cost of regraveling has led to consideration of upgrading such roads to surfaced standard, even though traffic volumes do not justify upgrading. Traditional chip seals are expensive and cannot be economically justified on roads that carry fewer than 500 vehicles per day. The KwaZulu–Natal Department of Transport is actively involved in efforts to identify cost-effective alternative surfacing products for low-volume roads. Field trials were conducted with Otta seals and Gravseals, which have been used successfully in other countries, as low-cost surfacing products for low-volume roads. The Otta seal is formed by placing graded aggregates on a relatively thick film of soft binder that, because of traffic and rolling, works its way through the aggregates. Gravseal consists of a special semipriming rubberized binder that is covered by a graded aggregate. Both Otta seals and Gravseals provide relatively flexible bituminous surfaces suitable for low-volume roads. Cost savings are derived mainly from the broad aggregate specifications, which allow for the use of marginal materials.

A Single Chip Integrated Navigation System

1993 ◽  
Vol 46 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Eric Aardoom ◽  
André Nieuwland
Keyword(s):  
Navigation System ◽  
System Integration ◽  
Cost Effective ◽  
Integrated System ◽  
Integrated Navigation ◽  
Single Chip ◽  
Integrated Navigation System ◽  
System Integrity ◽  
Cost Effective Approach ◽  
Application Specific

Recently, integration of different radionavigation systems has become very popular, since it improves system integrity, availability, accuracy and reliability. This paper discusses a new, flexible and cost-effective approach to system integration, centred on a single-chip application specific processor (ASP). An overview of this integrated system is presented and the application of the ASP for the implementation of a six-channel GPS, OMEGA, Loran-C and MLS receiver is given. The ASP is currently being implemented on a 180000 transistor 1·6μ, m CMOS Sea of Gates chip, and is expected to run at 100 MHz clock speed.

Fixation and staining of Drosophila L1 larval brains for immunofluorescence microscopy and preparation for live imaging

2020 ◽  
Author(s):  
Phuong Thao Ly ◽  
John D. Thompson ◽  
Sharyn A. Endow
Keyword(s):  
Immunofluorescence Microscopy ◽  
Cost Effective ◽  
Live Imaging ◽  
Permeable Membrane ◽  
Antibody Staining ◽  
First Instar ◽  
Stem Cell Quiescence ◽  
Cell Quiescence ◽  
3D Printed ◽  
Entire Procedure

Abstract Drosophila first instar (L1) larval brains (LBs) contain frequent quiescent neural stem cells (qNSCs) as well as activated neuroblasts, making them favorable for studying stem cell quiescence and activation. However, the small size of LBs at the L1 stage necessitates the use of modified methods to prepare the LBs for immunofluorescence microscopy (IFM). The protocol described here allows efficient collection of embryos and maturation of larvae to the mid-L1 stage, followed by dissection, fixation and processing of LBs through the antibody staining steps for IFM. The entire procedure can be completed in ~3-5 days. Methods are also described for use in preparing L1 brains for live imaging experiments, including a file to create accessible and cost-effective 3D printed slides that can be fit with an O2-permeable membrane for live imaging.

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