Colorimetric determination of urea v2

2021 ◽  
Author(s):  
noah.langenfeld not provided ◽  
Laurenpayne not provided ◽  
Bruce Bugbee
Keyword(s):  
Colorimetric Determination ◽  
Diacetyl Monoxime

This protocol measures the absorbance of urea in solution in complexation with diacetyl monoxime at 520 nm and is linearly proportional to concentration up to 5 mM urea.

scholarly journals Second-Derivative Spectrometric Determination of Urea in Milk Using the Diacetyl Monoxime Reagent

2010 ◽  
Vol 93 (2) ◽  
pp. 485-491 ◽  
Author(s):  
Steven A Nyanzi ◽  
Maureen Isiko ◽  
Francis Kateregga ◽  
Wolfgang Schwack
Keyword(s):  
Correlation Coefficients ◽  
Colorimetric Determination ◽  
Second Derivative ◽  
Linear Calibration ◽  
Colored Complex ◽  
Spectral Bands ◽  
Sensitivity And Selectivity ◽  
Diacetyl Monoxime ◽  
Second Derivative Method

Abstract The application of derivative spectrometry to resolve overlapping spectra and improve the sensitivity and selectivity of the colorimetric determination of urea in milk using diacetyl monoxime is presented. With first-derivative (1D) spectrometry, the max of the colored complex was established to be 525 nm. The absorption band at max 525 nm in normal absorption (0D) spectrometry was resolved into three clearly distinct spectral bands with minima at 497, 530, and 566 nm with second-derivative (2D) spectrometry. With the second-derivative (2D530) technique, the depth of the trough of the strongest signal at 530 nm was used to determine urea in milk samples. Linear calibration curves for urea were obtained over a concentration range of 0.21.4 mg/L, with correlation coefficients greater than 0.999. The LOD and LOQ of the method were 0.01 and 0.03 mg/L, respectively. Recoveries of 99100 of spiked urea in milk with RSD values below 2.0 were obtained. The second-derivative method is simple, affordable, sensitive, and selective for the determination of urea in milk.

The colorimetric determination of cyanate ion with hydroxylamine-diacetyl monoxime

1974 ◽  
Vol 23 (9) ◽  
pp. 1071-1073
Author(s):  
Keiichiro ISHII ◽  
Takeji IWAMOTO ◽  
Kazuhiko YAMANISHI
Keyword(s):  
Colorimetric Determination ◽  
Diacetyl Monoxime

A New Simple Semimicro Method for Colorimetric Determination of Urea

1963 ◽  
Vol 9 (1) ◽  
pp. 102-108 ◽  
Author(s):  
J J Coulombe ◽  
L Favreau
Keyword(s):  
Acid Medium ◽  
Rapid Method ◽  
Room Temperature ◽  
Colorimetric Determination ◽  
Red Color ◽  
Color Complex ◽  
Blood Filtrate ◽  
Diacetyl Monoxime

Abstract A new simple and rapid method for quantitative semimicro determination of blood urea is presented. Diacetyl monoxime and thiosemicarbazide form in acid medium with urea a red color complex with a maximum adsorption at 530 mµ stable at room temperature and requiring 0.2 ml. of 1:10 blood filtrate.

Optimization of conditions for the colorimetric determination of citrulline, using diacetyl monoxime

1980 ◽  
Vol 107 (2) ◽  
pp. 424-431 ◽  
Author(s):  
T.R.C. Boyde ◽  
Mohammed Rahmatullah
Keyword(s):  
Colorimetric Determination ◽  
Diacetyl Monoxime

scholarly journals Colorimetric determination of urea using diacetyl monoxime with strong acids

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259760
Author(s):  
Noah James Langenfeld ◽  
Lauren Elizabeth Payne ◽  
Bruce Bugbee
Keyword(s):  
Measurement Techniques ◽  
Color Stability ◽  
Colorimetric Determination ◽  
Diacetyl Monoxime ◽  
Simple Measurement ◽  
Wastewater Recycling ◽  
Strong Acids ◽  
Direct And Indirect Methods

Urea is a byproduct of the urea cycle in metabolism and is excreted through urine and sweat. Ammonia, which is toxic at low levels, is converted to the safe storage form of urea, which represents the largest efflux of nitrogen from many organisms. Urea is an important nitrogen source in agriculture, is added to many industrial products, and is a large component in wastewater. The enzyme urease hydrolyzes urea to ammonia and bicarbonate. This reaction is microbially mediated in soils, hydroponic solutions, and wastewater recycling and is catalyzed in vivo in plants using native urease, making measurement of urea environmentally important. Both direct and indirect methods to measure urea exist. This protocol uses diacetyl monoxime to directly determine the concentration of urea in solution. The protocol provides repeatable results and stable reagents with good color stability and simple measurement techniques for use in any lab with a spectrophotometer. The reaction between diacetyl monoxime and urea in the presence of sulfuric acid, phosphoric acid, thiosemicarbazide, and ferric chloride produces a chromophore with a peak absorbance at 520 nm and a linear relationship between concentration and absorbance from 0.4 to 5.0 mM urea in this protocol. The lack of detectable interferences makes this protocol suitable for the determination of millimolar levels of urea in wastewater streams and hydroponic solutions.

A modified diacetyl monoxime method for colorimetric determination of urea in soil extracts1

1979 ◽  
Vol 10 (8) ◽  
pp. 1163-1170 ◽  
Author(s):  
R. L. Mulvaney ◽  
J. M. Bremner
Keyword(s):  
Colorimetric Determination ◽  
Diacetyl Monoxime

A Method for the Colorimetric Determination of β-Glucuronidase in Urine, Serum, Tissue; Assay of Enzymatic Activity in Health and Disease

1959 ◽  
Vol 36 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Julius A. Goldbarg ◽  
Esteban P. Pineda ◽  
Benjamin M. Banks ◽  
Alexander M. Rutenburg
Keyword(s):  
Enzymatic Activity ◽  
Colorimetric Determination ◽  
Health And Disease ◽  
Urine Serum

Colorimetric Determination of Fluoride in Drinking Groundwater using a Polymeric Zirconium Complex of 5-(2-Carboxyphenylazo)-8-Hydroxyquinoline

2013 ◽  
Vol 12 (7) ◽  
pp. 460-465
Author(s):  
Sameer Amereih ◽  
Zaher Barghouthi ◽  
Lamees Majjiad
Keyword(s):  
Drinking Water ◽  
Detection Limit ◽  
Complex Formation ◽  
Molar Absorptivity ◽  
Colorimetric Determination ◽  
Zirconium Complex ◽  
Reliable Determination ◽  
Percentage Recovery ◽  
Quantitation Limit

A sensitive colorimetric determination of fluoride in drinking water has been developed using a polymeric zirconium complex of 5-(2-Carboxyphenylazo)-8-Hydroxyquinoline as fluoride reagents. The method allowed a reliable determination of fluoride in range of (0.0-1.5) mg L-1. The molar absorptivity of the complex formation is 7695 ± 27 L mol-1 cm-1 at 460 nm. The sensitivity, detection limit, quantitation limit, and percentage recovery for 1.0 mg L-1 fluoride for the proposed method were found to be 0.353 ± 0.013 μg mL-1, 0.1 mg L-1, 0.3 mg L-1, and 101.7 ± 4.1, respectively.

Colorimetric Determination of Amoxicillin in Pure and some Pharmaceutical Formulations Via Reaction with Potassium Permanganate as Oxidant Reagent

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Abbas Shebeeb Al-kadumi ◽  
Sahar Rihan Fadhel ◽  
Mohammed Abdullah Ahmed ◽  
Luma Amer Musa
Keyword(s):  
Potassium Permanganate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Atomic Emission ◽  
Basic Medium ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Spectrophotometric Methods ◽  
Label Claim

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

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