Bacterial isolation and genomic DNA extraction of Mycobacterium avium from pig lymph nodes v1

2021 ◽  
Author(s):  
Tetsuya Komatsu ◽  
Kenji Ohya ◽  
Justice Opare Odoi ◽  
Shota Suganuma ◽  
Kotaro Sawai ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Dna Extraction ◽  
Mycobacterium Avium ◽  
Genome Analysis ◽  
Genomic Dna ◽  
Mycobacterial Infection ◽  
Bacterial Isolation ◽  
Genomic Dna Extraction ◽  
Source Of Infection ◽  
Potential Source

Mycobacterium avium subsp. hominissuis (MAH) is one of the most important agents causing non-tuberculosis mycobacterial infection in human and pigs. Genome analysis on MAH of human isolates has been proceeding, however, those of pigs are limited despite its potential source of infection to human. Here, we isolated MAH from pig lymph nodes or livers and obtained their genomes.

Genomic DNA extraction from Mycobacterium avium v1 (protocols.io.bupvnvn6)

protocols.io ◽  
2021 ◽  
Author(s):  
Tetsuya Komatsu ◽  
Kenji not provided ◽  
Justice not provided ◽  
Shota not provided ◽  
Kotaro not provided ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Mycobacterium Avium ◽  
Genomic Dna ◽  
Genomic Dna Extraction

scholarly journals Genomic features of Mycobacterium avium subsp. hominissuis isolated from pigs in Japan.

2021 ◽  
Author(s):  
Fumito MARUYAMA ◽  
Tetsuya Komatsu ◽  
Kenji Ohya ◽  
Atsushi Ota ◽  
Yukiko Nishiuchi ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Virulence Genes ◽  
Draft Genome ◽  
Mycobacterial Infection ◽  
Epidemiological Studies ◽  
Genomic Features ◽  
Source Of Infection ◽  
Potential Source ◽  
Short Palindromic Repeat ◽  
Restriction Modification

Mycobacterium avium subsp. hominissuis (MAH) is one of the most important agents causing non- tuberculosis mycobacterial infection in humans and pigs. Genome analysis on MAH of human isolates has been proceeding, however, those of pigs are limited despite its potential source of infection to human. In the current study, we obtained 30 draft genome sequences of MAH of pigs reared in Japan. The 30 draft genomes consisted of 4,848,678 _ 5,620,788 bp length, 4,652 _ 5,388 coding genes and 46 _ 75 (Med: 47) tRNAs. All isolates had restriction modification associated genes and 185 _ 222 predicted virulence genes. Two isolates had tRNA arrays and one isolate had a clustered regularly interspaced short palindromic repeat (CRISPR) region. Our results will be useful for evaluation of the ecology of MAH by providing a foundation for genome-based epidemiological studies.

Optimization of genomic DNA extraction from formalin-fixed fish specimens

2013 ◽  
Vol 19 (6) ◽  
pp. 1068-1073
Author(s):  
Xiaolan KONG ◽  
Zuozhi CHEN ◽  
Lin LIN ◽  
Chunhou LI ◽  
Peiwen LIANG
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction ◽  
Formalin Fixed

Optimization of genomic DNA extraction from grapevine cultivars

2012 ◽  
Vol 29 ◽  
pp. S220
Author(s):  
Karlygash Aubakirova ◽  
Madina Omasheva ◽  
Natalya Ryabushkina ◽  
Laura Yerbolova ◽  
Tolepbergen Tazhibaev ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction

scholarly journals Optimization of BY2 cell suspension as a stable transformable system

2014 ◽  
Vol 42 (2) ◽  
pp. 472-477 ◽  
Author(s):  
Zhou SHUMIN ◽  
Chu YANXIA ◽  
Zheng BANG ◽  
Zhang WEI
Keyword(s):  
Cell Suspension ◽  
Dna Extraction ◽  
Genomic Dna ◽  
35S Promoter ◽  
Gfp Expression ◽  
Genomic Dna Extraction ◽  
Agrobacterium Mediated Transformation ◽  
Culture Cells ◽  
Low Efficiency

Tobacco (Nicotiana tabacum) cv. ‘Bright Yellow 2’ (BY2) cell suspension is a useful system to study the structure and function of plant cell. However, low efficiency of Agrobacterium-mediated transformation, and transgene silencing during subculture limit its application. Here we present optimization of the traditional protocols of Agrobacterium-mediated transformation and genomic DNA extraction. The transforming efficiency and recovery ratio of genomic DNA extraction were substantially increased by these improvements. Southern assay demonstrated that copy number of transgene could be determined unambiguously. Meanwhile by monitoring the GFP fluorescence we found that the GFP expression can keep stable in suspension culture cells for at least 20 days in liquid medium. Finally, applicability of constitutive promoters of Arabidopsis thaliana UBIQUITIN10 (AtUBQ10) and ARABIDOPSISSKP1 HOMOLOGUE1 (AtASK1) also can drive stable GFP expression in vivo of BY2 cells like CaMV 35S promoter in this plant system./span>

Genomic DNA extraction from herbarium samples of Cunila D. Royen ex L. (Lamiaceae) and Polygala L. (Polygalaceae)

2010 ◽  
Vol 3 (1) ◽  
pp. 37-39 ◽  
Author(s):  
Gustavo Agostini ◽  
Raquel Lüdtke ◽  
Sergio Echeverrigaray ◽  
Tatiana Teixeira de Souz-Chies
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Genomic Dna Extraction
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