Correlation of serological markers with haematological parameters in early diagnosis of dengue infection in dengue prone areas

Abstract: Early diagnosis of dengue fever and COVID-19 is made very easy due to technological advancements. The non-structural protein 1 antigen test strips are widely used in various regions; however, false-positive events have begun to be reported in the dengue-endemic areas with the COVID-19 pandemic, even though statistically non-structural protein 1 antigens are very specific to dengue infection. We reported a case of the false-positive non-structural protein 1 test in a patient with COVID-19 infection.

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A Proposal of Early Diagnosis and Early Management in Dengue Infection and Possible COVID-19

Exploratory Research and Hypothesis in Medicine ◽

10.14218/erhm.2020.00059 ◽

2020 ◽

Vol 000 (000) ◽

pp. 1-11

Author(s):

Manote Arpornsuwan ◽

Matinun Arpornsuwan

Keyword(s):

Early Diagnosis ◽

Dengue Infection ◽

Early Management

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STUDY OF NS1 ANTIGEN DETECTION AND ASSOCIATION OF PLATELET COUNT WITH VARIOUS DENGUE SEROLOGICAL MARKERS IN DENGUE INFECTION: AN INSTITUTIONAL EXPERIENCE"

SRMS Journal of Medical Science ◽

10.21761/jms.v1i2.7357 ◽

2016 ◽

Vol 1 (2) ◽

Author(s):

Neelam Gupta ◽

Rahul Kumar Goyal

Keyword(s):

Platelet Count ◽

Dengue Infection ◽

Dengue Shock Syndrome ◽

Accurate Diagnosis ◽

Serological Markers ◽

Ns1 Protein ◽

New Approach ◽

Ns1 Antigen ◽

Institutional Experience ◽

Initiation Of Therapy

Introduction: Dengue is a mosquito-borne disease caused by dengue virus. Annually, it affects up millions of people worldwide. An early and accurate diagnosis of dengue in the acute phase of illness is important for identifying an epidemic and for initiation of therapy. Detection of the secreted NS1 protein is a new approach that aid in the early diagnosis. Platelet count is the only non-dengue parameter that can support the diagnosis of the dengue shock syndrome and dengue. This study was done to detect dengue parameters and correlate them with the platelet count. Material and Methods: This study was conducted from September 2016 to November 2016. Total 205 blood samples were collected from clinically suspected dengue cases. Serum was separated and tested for NS1 antigen, IgM and IgG using the immunochromatography kit. The platelet count was also recorded in all samples. Results: Out of 205 samples tested total 92(44.9%) specimens were positive for one or more dengue parameters. Among 92 specimens 45(49%) were positive for only NS1 antigen and 20 were positive for IgM only. Thrombocytopenia was recorded in 73(79%)out of 92 dengue positive cases. Conclusion: Inclusion of NS1 in the diagnosis of dengue increases the detection rate significantly in early phase of the infection.

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Haematological Parameters in Early Diagnosis of Clinically Suspected Neonatal Septicemia

Myanmar Health Sciences Research Journal ◽

10.34299/mhsrj.00924 ◽

2019 ◽

Vol 31 (1) ◽

pp. 65-71

Keyword(s):

Early Diagnosis ◽

Late Onset ◽

Cost Effective ◽

Haematological Parameters ◽

Predictive Values ◽

Neonatal Septicemia ◽

Mature Neutrophil ◽

Life Threatening ◽

Low Sensitivity ◽

Sensitivity Specificity

Neonatal septicemia is a life-threatening yet treatable condition. It is one of the major health problems throughout the world. Blood culture is the gold standard for diagnosis of neonatal septicemia and should be performed in all cases of suspected septicemia prior to starting antibiotics. But in the present study, the use of the haematological parameters was evaluated to determine the early diagnosis of neonatal septicemia. It was carried out in the neonatal unit of Mandalay Children’s Hospital from September 2012 to August 2013. Out of 68 cases of clinically suspected neonatal septicemia, bacterial pathogens were isolated from 33 cases. Early onset neonatal septicemia was more common than late onset. Among 33 cases of culture-proven neonatal septicemia, septicemia is not likely in 1 case (3%) while borderline for septicemia and septicemia in 6 cases (18.2%) is very likely in 26 cases (78.8%) according to haematological scoring system (HSS). HSS assigns a score of one for each of seven haematological parameters. Out of the seven haematological parameters, immature to total neutrophil ratio (I:T) and immature to mature neutrophil ratio (I:M) were the most reliable test for the neonatal septicemia. Thrombocytopenia had low sensitivity. The haematolgical parameters are simple, quick, cost effective and readily available. The sensitivity, specificity and the predictive values of haematological parameters could be enhanced by combination of these parameters rather than the use of single parameter.

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Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Sensors ◽

10.3390/s18082641 ◽

2018 ◽

Vol 18 (8) ◽

pp. 2641 ◽

Cited By ~ 6

Author(s):

Daniel Wasik ◽

Ashok Mulchandani ◽

Marylynn Yates

Keyword(s):

Early Diagnosis ◽

Dengue Virus ◽

Dengue Infection ◽

Denv Infection ◽

Human Saliva ◽

Diagnostic Methods ◽

Label Free ◽

Ns1 Protein ◽

Structural Protein ◽

Blood Draw

Dengue virus (DENV) is a highly pathogenic, arthropod-borne virus transmitted between people by Aedes mosquitoes. Despite efforts to prevent global spread, the potential for DENV epidemics is increasing world-wide. Annually, 3.6 billion people are at risk of infection. With no licensed vaccine, early diagnosis of dengue infection is critical for clinical management and patient survival. Detection of DENV non-structural protein 1 (NS1) is a clinically accepted biomarker for the early detection of DENV infection. Unfortunately, virtually all of the laboratory and commercial DENV NS1 diagnostic methods require a blood draw for sample analysis, limiting point-of-care diagnostics and decreases patient willingness. Alternatively, NS1 in human saliva has been identified for the potential early diagnosis of DENV infection. The collection of saliva is simple, non-invasive, painless, and inexpensive, even by minimally trained personnel. In this study, we present a label-free chemiresistive immunosensor for the detection of the DENV NS1 protein utilizing a network of single-walled carbon nanotubes functionalized with anti-dengue NS1 monoclonal antibodies. NS1 was successfully detected in adulterated artificial human saliva over the range of clinically relevant concentrations with high sensitivity and selectivity. It has potential application in clinical diagnosis and the ease of collection allows for self-testing, even within the home.

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Clinical and haematological parameters for the early diagnosis of pneumonia in foals

Pferdeheilkunde Equine Medicine ◽

10.21836/pem20180307 ◽

2018 ◽

Vol 34 (3) ◽

pp. 260-266

Author(s):

R Thomé ◽

K Rohn ◽

M Venner

Keyword(s):

Early Diagnosis ◽

Haematological Parameters

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DENGUE FEVER: CLINICO-PATHOLOGICAL CORRELATION WITH SEROLOGICAL MARKERS AND PLATELET COUNT

10.36106/ijar/9102416 ◽

2021 ◽

pp. 1-2

Author(s):

Gunjan Jain ◽

Saloni Savani ◽

Hardik Jain

Keyword(s):

Platelet Count ◽

Dengue Fever ◽

Rna Virus ◽

Dengue Infection ◽

White Blood Cells ◽

Hemorrhagic Fever ◽

Serological Markers ◽

Platelet Counts ◽

Microbiological Laboratory ◽

Viral Illness

Dengue is an acute viral illness caused by RNA virus of the family Flaviviridae and spread by Aedes mosquitoes. Presenting features may range from asymptomatic fever to dreaded complications such as hemorrhagic fever and shock. A decreased number of white blood cells (leukopenia) accompanied by a decreased number of platelet count (thrombocytopenia) and metabolic acidosis are the initial changes on laboratory examinations. Microbiological laboratory testing conrms the diagnosis of Dengue Fever. Virus segregation in cell cultures, nucleic acid demonstration by polymerase chain reaction (PCR), and serological detection of viral antigens (such as NS1) or particular antibodies are the preferred microbiological assays. This study focuses on the importance of platelet counts and other platelet parameters in dengue infection. Also platelet counts are compared with clinical, radiological and other serological markers.

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