scholarly journals Case Report: False-Positive Dengue Non-Structural Protein 1 Antigen in a Patient with COVID-19 Infection

2020 ◽  
Vol 26 (2) ◽  
Author(s):  
Yohanes Firmansyah ◽  
Jessica Elizabeth ◽  
Hendsun Hendsun ◽  
Darren Gosal
Keyword(s):  
Case Report ◽  
Early Diagnosis ◽  
Dengue Fever ◽  
False Positive ◽  
Dengue Infection ◽  
Antigen Test ◽  
Structural Protein ◽  
Positive Events ◽  
Test Strips ◽  
Endemic Areas

Abstract: Early diagnosis of dengue fever and COVID-19 is made very easy due to technological advancements. The  non-structural protein 1 antigen test strips are widely used in various regions; however, false-positive events have begun to be reported in the dengue-endemic areas with the COVID-19 pandemic, even though statistically non-structural protein 1 antigens are very specific to dengue infection. We reported a case of the false-positive non-structural protein 1 test in a patient with COVID-19 infection.

Study on Clinical Spectrum, Laboratory Profile and Outcome of Dengue Fever in Adults

Mediscope ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 33-39
Author(s):  
Muhammad Ashraful Kabir ◽  
Syed Didarul Haque ◽  
Baishakhi Islam ◽  
Hasan Imam
Keyword(s):  
Abdominal Pain ◽  
Pleural Effusion ◽  
Dengue Fever ◽  
Laboratory Finding ◽  
Dengue Infection ◽  
Febrile Illness ◽  
Clinical Spectrum ◽  
Structural Protein ◽  
Careful Clinical Examination ◽  
Laboratory Profile

Background: Dengue infection is a major health burden, which can result in mild self-limited febrile illness to highly fatal haemorrhagic disease. Infection is caused by Dengue virus, which is transmitted by the Aedes aegypti mosquito. Objective: The aim of the study was to see the clinical spectrum, laboratory profile and outcome of dengue fever in adult. Methods: This prospective observational study was carried out on 75 patients in the department of medicine of Bangabandhu Sheikh Mujib Medical University, Dhaka from 11/07/2019 to 20/10/2019. All patients above 18 years with confirmed dengue, who were either hospitalized or managed as outdoor patients with NS1 (non-structural protein) antigen and/ or IgM dengue antibody positive were included in the study. The patients with concomitant malaria, typhoid and leptospirosis were excluded from the study. Detailed history and careful clinical examination were performed on each patient. Results: In this study, all patients (100%) had fever. Among them, 70 (93.3%) was suffering from headache, 66 (88.0%) myalgia, 32 (42.7%) abdominal pain, 30 (40.0%) conjuctival suffusion, 29 (38.7%) nausea/vomiting, 27 (36.0%) skin rashes, 14 (18.7%) pleural effusion, 13 (17.3%) ascites, 13 (17.3%) retro-orbital pain, 11 (14.7%) itching, 8 (10.7%) hepatomegaly, 7 (9.3%) splenomegaly. Death occurred in 3 (4.0%) patients. Rate of cure was 58 (77.3%) and ICU admission was required in 14 (18.7%), they were ultimately cured. Conclusion: Fever associated with headache and myalgia were the most common symptoms. Other common clinical features were abdominal pain, conjunctival suffusion, nausea/vomiting, skin rashes and pleural effusion. Regarding laboratory finding, platelet count has little role in management of dengue patients. Mediscope Vol. 8, No. 1: January 2021, Page 33-39

scholarly journals Salivary Detection of Dengue Virus NS1 Protein with a Label-Free Immunosensor for Early Dengue Diagnosis

Sensors ◽  
2018 ◽  
Vol 18 (8) ◽  
pp. 2641 ◽  
Author(s):  
Daniel Wasik ◽  
Ashok Mulchandani ◽  
Marylynn Yates
Keyword(s):  
Early Diagnosis ◽  
Dengue Virus ◽  
Dengue Infection ◽  
Denv Infection ◽  
Human Saliva ◽  
Diagnostic Methods ◽  
Label Free ◽  
Ns1 Protein ◽  
Structural Protein ◽  
Blood Draw

Dengue virus (DENV) is a highly pathogenic, arthropod-borne virus transmitted between people by Aedes mosquitoes. Despite efforts to prevent global spread, the potential for DENV epidemics is increasing world-wide. Annually, 3.6 billion people are at risk of infection. With no licensed vaccine, early diagnosis of dengue infection is critical for clinical management and patient survival. Detection of DENV non-structural protein 1 (NS1) is a clinically accepted biomarker for the early detection of DENV infection. Unfortunately, virtually all of the laboratory and commercial DENV NS1 diagnostic methods require a blood draw for sample analysis, limiting point-of-care diagnostics and decreases patient willingness. Alternatively, NS1 in human saliva has been identified for the potential early diagnosis of DENV infection. The collection of saliva is simple, non-invasive, painless, and inexpensive, even by minimally trained personnel. In this study, we present a label-free chemiresistive immunosensor for the detection of the DENV NS1 protein utilizing a network of single-walled carbon nanotubes functionalized with anti-dengue NS1 monoclonal antibodies. NS1 was successfully detected in adulterated artificial human saliva over the range of clinically relevant concentrations with high sensitivity and selectivity. It has potential application in clinical diagnosis and the ease of collection allows for self-testing, even within the home.

scholarly journals Neonatal dengue

2017 ◽  
Vol 4 (6) ◽  
pp. 2234 ◽  
Author(s):  
Jessy Thomas ◽  
Priya Thomas ◽  
Chinnu Reeba George
Keyword(s):  
Case Report ◽  
Birth Weight ◽  
Dengue Fever ◽  
Perinatal Death ◽  
Early Recognition ◽  
Dengue Infection ◽  
Perinatal Transmission ◽  
Emergency Caesarean Section ◽  
Supportive Treatment ◽  
Female Baby

Dengue infection is possible in all three trimesters of pregnancy. Dengue during pregnancy is associated with several neonatal complications such as perinatal death, miscarriage, low birth weight and preterm delivery. This is a case report of perinatal transmission of dengue in a baby who was born by an emergency caesarean section. The female baby was diagnosed with dengue fever as her dengue NS1 and ELISA tests were positive like her mother. Early recognition of congenital dengue will significantly reduce maternal and infant mortality. Proper management and supportive treatment is required for thrombocytopenia and hemorrhagic manifestations in dengue fever. 

scholarly journals Co-infection with Legionella and SARS-CoV-2: a case report

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Masaru Shimizu ◽  
Yusuke Chihara ◽  
Sakiko Satake ◽  
Astuko Yone ◽  
Mari Makio ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Case Report ◽  
Early Diagnosis ◽  
Tracheal Intubation ◽  
Early Treatment ◽  
Antibacterial Agents ◽  
Multidisciplinary Care ◽  
Antigen Test ◽  
Laboratory Findings ◽  
Positive Urine

Abstract Introduction We report a case of COVID-19 with Legionella co-infection that was treated successfully. Case report A 73-year-old man presented to the hospital with symptoms of fatigue that continued for the next 5 days. The patient was receiving docetaxel and prednisolone chemotherapy for prostate cancer. Laboratory findings on admission showed positive urine Legionella antigen test and SARS-CoV-2 test. He was administered antiviral and antibacterial agents, and a corticosteroid. Pneumonia exacerbated on day 2 of hospitalization. The patient underwent tracheal intubation and began receiving multidisciplinary care. On day 8 of hospitalization, his oxygenation improved, and the patient was extubated. He discharged on day 27 of hospitalization. Conclusions The patient had a favorable outcome with early diagnosis and early treatment of both diseases. Patients with severe COVID-19 disease need to be evaluated for co-infection. Further, early diagnosis and early treatment of the microbial bacteria causing the co-infection are important.

Clinical and Laboratory Profile of Dengue Fever in the Paediatric Age Group at a Tertiary Care Hospital of West Bengal - A Comparison of Two Subsequent Years

2021 ◽  
Vol 8 (25) ◽  
pp. 2162-2167
Author(s):  
Shankar Sikdar ◽  
Ujjwal Bandyopadhyay ◽  
Sutapa Das ◽  
Mala Bhattacharya
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Warning Signs ◽  
Health Concern ◽  
Care Hospital ◽  
Age Group ◽  
Structural Protein ◽  
Asian Countries ◽  
Paediatric Age

BACKGROUND Dengue is a major public health concern in tropical Asian countries and a most rapidly spreading mosquito borne viral disease with a 30 fold increase in global incidence over the last five decades. Dengue is among the ten leading causes of death in children ranging from 1 to 15 years of age in tropical Asian countries. Complications of dengue fever are common in children. But very limited studies are available on paediatric populations. The virus or non-structural protein (NS1) interacts with platelets and coagulation factors. A combined effect of mild disseminated intravascular coagulation, liver damage and platelet dysfunction results in bleeding in dengue fever. With this scientific knowledge background, the present study was undertaken to identify and document the changing pattern of dengue infection in paediatric age group with a special emphasis on laboratory findings of dengue cases in paediatric age group. METHODS The present descriptive study was conducted for two years in a state run paediatric referral hospital. The sample was purposive by design and consisted of patients who have been diagnosed as having dengue fever with warning signs and admitted. RESULTS Fever was typically high grade (> 103 F) and most patients presented with warning signs within 5 days of illness (64.2 % in year 2016 and 76.6 % in year 2017). Persistent vomiting and abdominal pain were the most common warning signs in both the years. Thrombocytopenia was the commonest laboratory finding. Bleeding in study subjects lead to < 50000 / cu mm platelet count. Most common bleeding was gastrointestinal bleeding (39.2 % of study subjects with platelet count < 50000 / cu mm presented with G. I bleeding). CONCLUSIONS Dengue illness comes with varied presentation. Atypical presentations can delay the diagnosis. Early recognition of warning signs keeping the atypical presentation in mind is important. KEYWORDS Dengue Fever, Paediatric Age Group, Non-Structural Protein (NS1), Thrombocytopenia

scholarly journals OVERVIEW OF NUCLEAR FACTOR-KB (NF-KB) AND NON-STRUCTURAL PROTEIN 1 (NS1) IN PATIENTS WITH DENGUE FEVER IN PREMIER HOSPITAL, SURABAYA

2019 ◽  
Vol 7 (5) ◽  
pp. 109
Author(s):  
Ni Nyoman Budiutari ◽  
Yoes Prijatna Dachlan ◽  
Jusak Nugraha
Keyword(s):  
Transcription Factors ◽  
Viral Replication ◽  
Dengue Fever ◽  
Interleukin 1 ◽  
Dengue Infection ◽  
Sandwich Elisa ◽  
Rapid Test ◽  
Host Cells ◽  
Ns1 Protein ◽  
Structural Protein

Dengue fever (DF) is an acute viral fever caused by RNA virus that is transmitted by Aedes aegypti and Aedes albopictus mosquitoes. DF is also called viral arthropod-borne disease and is accompanied by headaches, joint and muscle pain. The main target of dengue infection is macrophages or monocytes and dendritic cells (DC). Infected DC is caused the viral replication and the endocytosis into endosomal, easier, thus inducing the activation of NF-ĸB transcription factor to produce proinflammatory cytokines such as Tumor Necrosis Factor-α (TNF-α), Interleukin-1 (IL-1), IL-6, IL-12 and chemokine. NF-kB is one of the transcription factors involved in the regulation of the expression of various cytokines, chemokines and anti/pro-apoptotic proteins during infection and act as indicator of disease severity. Infected DC cells are secreted NS1 protein which is the co-factor needed for viral replication and can be detected in the first eight days. The level will be higher in the initial phase of fever. The purpose of this study was to analyze the description of NF-kB and NS1 levels in the serum of patients with dengue fever through observational analytic studies through a cross-sectional approach. This study was done on 40 patients with dengue fever and 10 healthies people as negative controls. NS1 was analyzed in serum of Panbio rapid test and NF-kB level were measured by sandwich ELISA. The results are showed positive and negative NS1 results in dengue fever patients. The average NF-kB serum level in dengue fever patients was found to be higher than the control. NF-ĸB level in negative NS1 was higher than the NS1 positive group. It is showed that NS1 is detected both in the acute phase. The detection of NF-ĸB is showed the involvement of transcription factors in the development of dengue virus infection and has a protective role for host cells.

scholarly journals THE STUDY OF DETECTION OF DENGUE CASES BY NS1 ANTIGEN AND IGM ANTIBODY IN RIMS, ADILABAD, INDIA.

2019 ◽  
Vol 3 (11) ◽  
Author(s):  
Dr. Tanajee Zade ◽  
Dr. K. Srinivas ◽  
Dr. Akshay Berad
Keyword(s):  
Early Diagnosis ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Tertiary Care ◽  
High Morbidity ◽  
Care Hospital ◽  
Serum Samples ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen

Dengue fever is an acute febrile arboviral disease affecting tropical & subtropical regions of the world. Dengue infection produces a spectrum of clinical illness, ranging from an asymptomatic to its most severe form like dengue haemorrhagic fever and dengue shock syndrome. In view of high morbidity and mortality, it is imperative to have a rapid and sensitive laboratory assay for early detection of the dengue infection. The newer parameter NS1 antigen has gained a lot of interest for early diagnosis of the disease. Detection of non-structural antigen (NS1 Ag), IgM and IgG antibody may help in the early diagnosis. The present study was conducted in a RIMS Adilabad, tertiary care hospital & medical college in the Department of  General Medicine. A total of 100 serum samples were processed from suspected cases of dengue fever by using dengue test for detection of NS1 antigen and IgG antibodies. Platelet counts of all these cases were noted. . Of these 100 subjects 85 were serologically proved to have dengue illness, 57 patients were NS1 antigen positive, 28 patients were IgM antibody positive patients. As the NS1 antigen is detectable in blood from day one after onset of fever, its assay is an effective tool for early diagnosis of dengue infection so as to avoid complications. Key words:  Dengue, NS1 Antigen, IgM antibody, Platelet

scholarly journals Correlation of C-reactive protein and neutrophil counts as early indicators of severe dengue in children

2017 ◽  
Vol 4 (2) ◽  
pp. 450
Author(s):  
Sandhya Rani Atukuri ◽  
Praveen Nayak
Keyword(s):  
Early Diagnosis ◽  
Dengue Fever ◽  
Dengue Infection ◽  
Predictive Markers ◽  
Warning Signs ◽  
Severe Neutropenia ◽  
Severe Dengue ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Study Population

Background: Dengue has broad clinical presentation with unpredictable clinical evolution and outcome while most patients recover following a self-limiting non-severe clinical course, a small proportion progress to severe disease. As early diagnosis of dengue infection remains a challenge around the world in areas of limited resources, laboratory parameters like CRP, Neutrophil counts may serve as predictive markers to promote early diagnosis. The objectives of this study were to stratify the levels of C-reactive protein and Neutrophil counts in children with dengue fever and to determine the correlation of C-reactive protein and Neutrophil counts with the severity of dengueMethods: This was an Observational chart based descriptive study done in all pediatric dengue children (aged 1-15 years) admitted at Father Muller medical college hospital, Mangalore during the period of June 2014 to 2016. Total sample size was 100. Data collection was done by using purposive sampling based on inclusion and exclusion criteria from case records. Controls (n = 20), children with diagnosis of viral fever were considered in the study.Results: Out of the 100 children studied, 16% were <5 years ,33% between 6-10 years and 51% above 10 years of age. Mean CRP levels in NS1, IgM and both are 6.2, 6.9, 6.3. CRP values >5 were considered as positive. Mean CRP values in dengue fever with warning signs (DF1), without warning signs (DF2) and Severe Dengue (DF3) were 6.3, 6.2, 11.4 respectively. Absolute Neutropenia was observed in 52% of the study population of dengue (DF1) of which severe neutropenia was observed in 19% and 15% OF DF2 (with no warning signs) also showed neutropenia. CRP values are significant in the study when compared to controls and absolute neutropenia was observed in 52% of dengue with warning signs. Hence CRP and neutropenia may be helpful as early predictors of severe dengue.Conclusions: Our study was an attempt to correlate CRP and neutropenia for early prediction of severe dengue. Mean CRP values in the population were significant statically but not as markedly elevated in other bacterial illness as the study population have no enrolled cases of severe dengue. But neutropenia (<1,500/cmm) and positive CRP (>5) may serve as predictive markers in a resource limited setting. 

scholarly journals Study of clinical and laboratory profile of dengue fever in a tertiary care hospital

2020 ◽  
Vol 7 (2) ◽  
pp. 222
Author(s):  
Sangram S. Mangudkar ◽  
Sachin K. Shivnitwar ◽  
Atiullah I. Malik
Keyword(s):  
Dengue Fever ◽  
Biochemical Parameters ◽  
Tertiary Care Hospital ◽  
Dengue Infection ◽  
Tertiary Care ◽  
Care Hospital ◽  
Conjunctival Injection ◽  
Structural Protein ◽  
Considerable Morbidity ◽  
Morbilliform Rash

Background: Dengue fever is one of the most common arboviral mediated outbreaks reported with increased prevalence over the last few years with considerable morbidity and mortality. This study was designed to study the clinical and biochemical parameters in dengue fever patients.Methods: Prospective observational study was undertaken among adult patients in a tertiary care hospital. fifty patients were studied and analysed. All patients who were NS1 (Non-Structural Protein 1) antigen or IgM dengue positive were included in the study. Clinical features, haematological and biochemical parameters were noted.Results: Of the 50 patients studied, majority were males (68%). Fever was the major symptom (100%) followed by Body ache (84%), Headache (64%), Retro-orbital pain (52%), Myalgia (48%), conjunctival injection (40%), Itching (40%), abdominal pain (36%), Bradycardia (34%), Rash (30%), pleural effusion and ascites both seen in (28%). Significant derangements in platelet (76%), leucocyte counts (84%) and serum transaminases (58%) were noted.Conclusions: Fever associated with headache, retroorbital pain, erythematous morbilliform rash, conjunctival injection and itching over palms and soles along with thrombocytopenia, leukopenia, elevated liver transaminases should prompt a clinician on the possibility of dengue infection. Platelet transfusions have little role in management of dengue patients unless patients having active bleeding secondary to thrombocytopenia due to dengue fever.

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